LOCUS       sequence-49403-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Chen JK, Taipale J, Cooper MK, Beachy PA
  TITLE     Inhibition of Hedgehog signaling by direct binding of cyclopamine to
            Smoothened.
  JOURNAL   Genes Dev. 2002 Nov 1. 16(21):2743-8.
  PUBMED    12414725
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2002 Nov 1. 16(21):2743-8."
COMMENT     Sequence Label: pGE-mSmo-YFP
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(206..457)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(248..269)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     597..616
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag ttatctagat
      121 ccggtggatc ccgggcccgc ggtaccgtcg actacgacct aattcctgca gaattcgaag
      181 cttgagctcg agatctgagt ccggacttgt acagctcgtc catgccgaga gtgatcccgg
      241 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
      301 gggcggactg gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
      361 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
      421 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
      481 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      541 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      601 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
      661 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag cactgcaggc
      721 cgtagccgaa ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtg
//
