LOCUS       sequence-494103-       14058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses split luciferase fusion cLUC-NLRa in planta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14058)
  AUTHORS   Chen R, Chen J, Outram MA, Powell OR, Arndell T, Gajendiran K, Wang 
            YL, Ayliffe MA, Blundell C, Figueroa M, Sperschneider J, Vanhercke 
            T, Tang D, Xu Y, Zhong G, Gardener C, Yu G, Gourdoupis S, Jaremko Ł,
            Matny O, Steffenson BJ, Boshoff WHP, Meyer WB, Arold ST, Dodds PN, 
            Wulff BBH
  TITLE     A wheat tandem kinase sensor activates an NLR helper to trigger 
            immunity
  JOURNAL   bioRxiv 2024.08.30.610287
REFERENCE   2  (bases 1 to 14058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.08.30.610287"
COMMENT     Sequence Label: pCambia1300_cLUC-NLRa
FEATURES             Location/Qualifiers
     source          1..14058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        501..846
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      4512..5153
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(5161..5178)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5161..5177)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5170..5192)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5380..5404
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6705..7334
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7763..8836
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8902..9096
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9332..9354)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9440..9580
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9495..9514
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9595..9612)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9766..10354)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9846..9865)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10441..11235)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11660..11684
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11762..11936
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11977..13002)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(13069..13745)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    13936..13957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13972..13977,13978..13995,13996..14002)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14010..14026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14015..14037
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14034..14050
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14034..14050
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaattcggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt
       61 agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa
      121 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
      181 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
      241 caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
      301 cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc
      361 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc
      421 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc
      481 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc
      541 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc
      601 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
      661 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
      721 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
      781 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
      841 agaacacggg ggacgagctc atgtccggtt atgtaaacaa tccggaagcg accaacgcct
      901 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
      961 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc
     1021 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag
     1081 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
     1141 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
     1201 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
     1261 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgg
     1321 gaggtggctc atctggcgga ggtcagatct cgtacgcgtc ccggggcggt accggcatgg
     1381 aggagatcgt gcttggactg tccaagacgg tggtggaggg gacgctggtc aaggtgaagg
     1441 cggcgattga cgaggagtcg aggctgaagc tcgcggtgca gagcgacctg gtgttcatca
     1501 cgggggagtt cgagatgatg cagtcgttcc tcaacgtcgc cgacgcggag cgcatcaaga
     1561 acaacgccgt caagacgtgg gtgaggcagc tccgcgacct ggcctacgac accgaggact
     1621 gcatcgagct ggtcgtccac ctggatccca agcccagatg gtggcgccgc ctgctggtcc
     1681 tgccttgcct gccggcggtg tcgctgccaa tggatgacgc ggcggcggag ataaaggagc
     1741 tcaagggcag ggtggagttc gtgagccagc ggaatatgcg ctacaacctc atcaccgact
     1801 tcggcggcgc caagtccgcc gcgcagcagc agcttgaccg ggccgccgga gctctcgaca
     1861 tcgccgtgga ggcggcggcg aggaaagggc gtagcttggt ggatctgacc gagttgatcc
     1921 ccaaaatgga ggaccgccct gagctcggcg tgatctcggt gtggggcaca ggcggcgacc
     1981 ttggggtggc gtccatcgtc aggaaggcct acgacgaccc tgaaatacgc aagaacttcc
     2041 atagtcgtgg ctgggccaag ttgacccacc ctttcgatcc gcggaagatc ctccggagct
     2101 tgctcattca gttctgcact aacactgctg ctccacagaa gcaaggggag actctggatg
     2161 tagattcgtt gctgaagatg gaaaagacag tggtggaaga aggggagctc gtcaagcagt
     2221 ttgtgagtca tgtgagcacc cacaggttcc tcgtcgtcct cgaaggcgtg tcaacgatgg
     2281 cggaatggga tgccctccgg atgtacctgc cagacatggg taatgggagt cagatcatcg
     2341 tatccacaca gcagttcgac atcgcgagct tgtgcaccgg gcaactgcac aaagtttcag
     2401 agttgagaaa attcacacct gaccactctg tttgtgtgtt cttcaaggag gtttctggag
     2461 gtgatgatcc tggaaatgca atgctggagg gagatgacaa agctgaactt attgggcgtg
     2521 acacagaaga ggaggagctt atggagcaga ttgatgaggt ttcccgtgtg atctcggtat
     2581 gggggattgc tggtgtagga aaatcggcac ttgtcagaag tgcctactgc aagtatctcc
     2641 aggaccgccc caactttgct gggttaaatc tgacctacat acacgggcgc gtgaccgtgc
     2701 cccatccctt taatctgagg gagttctgcc gcagcttgct cctgaacttg tcctctgagc
     2761 ctgctaaaac caaagaggac gccgtcgctg aactggcaaa catgaccaat ccaactgatg
     2821 tctgccgtaa gcttcttagc gacgatcaac aacagaagca gtgccttatt gttattgatg
     2881 acctccagtc cactgaagaa tgggacagaa taaaacaggc cttgtcattt ggtaaagaat
     2941 gttgtatcat agttgttacg aatgattcaa gtgttgccaa atattgttca gggcaagatc
     3001 agaaactcat gttcaacgtc aaaggtcttg gtgacgagca tgcccttgac ctcttcaaca
     3061 cggtatatga cggcatgcct aaccacatca cacgggatga ggacatgatc aatcagtcga
     3121 agctaatgtt aaacaaatgc ggcggactac ccaaggtgat agttgcaatt ggtcgtttct
     3181 tggccaagag tctgaattgg gaggccatga atactaactt tattcaccag ttggaaaaca
     3241 acccagagct ggctagtata catagcctat ttagttggct ggactcctac ttccacaact
     3301 gtcctgatga actcaagcca tgcatcttct atttgtcaat cttccctcga cgccacggca
     3361 ttcgtcggag gcgtctggtg aggcggtgga tcgccgaggg ctactcaagg gacaccgacg
     3421 ggaatctcgc ggaagtgaac ggggaaaatt acttctccag gcttgtcaac ctcagcatgc
     3481 tcacagatgc tgagcgtggt gtcaacgcgg ctgggacagg gaggaaaatg gccatgtgca
     3541 acatcaacga tttcttccgg gagtacattg tgtcgcggcg aatggaggag aaccatgtgt
     3601 ttgcactgga ggggaggtgc agccagacca cacggcgcac tgggcggcac ctggtcatca
     3661 accaaagctg ggacggagat gagaacgtgt tcaacaggat cgagttctcg cgactgcggt
     3721 cggtgacagt gttcggaccc tggaagccat tcctggcctc cgacaagatg agggtgctcc
     3781 gcgtgcttga tctggaaggc actgaaggtc tgacagacga cgacatcaag agcatcgtca
     3841 aacggctgcc tcgactgaag ttcctctccc tgcgaaaatg caaaaacatc tttcggctgc
     3901 cgaagtccct gggtcgcctg aggcagctcc agaccctgga tgtgaggcac actgccatag
     3961 cccagctgcc agcgaccgtc gtgaagctac agaagttgca gtatgttcgt gccggtaaca
     4021 caccagagga atatgccggc agcgacagca cggaatctag cacctcggtg cccgcctgtt
     4081 tctcatgtgg ccctgttggc ggtcacattg tcggcgttga ggtgcctcgc ggggtcgaga
     4141 atctgaccaa cctgcacaca ctgggtgtca tcaaagccac tgtggcggga ctcaaggagc
     4201 tcaaaaagct tacccagctg cgcaagctcg gtgtgtctgg catcaaccgg agaaaccaca
     4261 aagagctctg tgccgtcgtc tccggacatg gtcatctgga atccctgtcg atatggcttg
     4321 acaaggacac cgaggggatt gctgaccctt gttcgccgag caatgatgac ttcaagcctc
     4381 cagagaagct acggaggctc aaactccacg ggcacatcgg caatttgcca gcatgggtta
     4441 gcactctcac catgctggat ttggtggaag tatccaagtc tgctgctcaa gatccatgag
     4501 tcgacctgca gagctttcgt tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc
     4561 atcagtttca ttgcgcacac accagaatcc tactgagttt gagtattatg gcattgggaa
     4621 aactgttttt cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt attcggtttt
     4681 cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat atggtccttt
     4741 tgttcattct caaattaata ttatttgttt tttctcttat ttgttgtgtg ttgaatttga
     4801 aattataaga gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct
     4861 aatgaccgaa gttaatatga ggagtaaaac acttgtagtt gtaccattat gcttattcac
     4921 taggcaacaa atatattttc agacctagaa aagctgcaaa tgttactgaa tacaagtatg
     4981 tcctcttgtg ttttagacat ttatgaactt tcctttatgt aattttccag aatccttgtc
     5041 agattctaat cattgcttta taattatagt tatactcatg gatttgtagt tgagtatgaa
     5101 aatatttttt aatgcatttt atgacttgcc aattgattga caacatgcat caagcttggc
     5161 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     5221 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     5281 cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca
     5341 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
     5401 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     5461 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
     5521 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     5581 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     5641 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     5701 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     5761 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     5821 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     5881 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     5941 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     6001 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     6061 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     6121 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     6181 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     6241 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     6301 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     6361 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     6421 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     6481 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     6541 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     6601 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     6661 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     6721 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     6781 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     6841 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     6901 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     6961 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     7021 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     7081 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     7141 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     7201 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     7261 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     7321 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     7381 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     7441 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     7501 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     7561 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     7621 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     7681 cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa
     7741 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     7801 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     7861 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     7921 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     7981 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     8041 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     8101 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     8161 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     8221 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     8281 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     8341 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     8401 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     8461 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     8521 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     8581 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     8641 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     8701 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     8761 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     8821 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     8881 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     8941 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     9001 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     9061 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     9121 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     9181 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     9241 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     9301 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     9361 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     9421 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     9481 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     9541 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     9601 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9661 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9721 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9781 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9841 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9901 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9961 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10021 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10081 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10141 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10201 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10261 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10321 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10381 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta
    10441 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
    10501 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
    10561 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
    10621 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
    10681 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
    10741 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
    10801 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
    10861 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
    10921 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
    10981 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
    11041 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
    11101 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
    11161 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
    11221 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
    11281 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
    11341 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
    11401 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
    11461 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
    11521 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
    11581 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
    11641 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
    11701 aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg
    11761 ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt
    11821 attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc
    11881 ctataggaac cctaattccc ttatctggga actactcaca cattattatg gagaaactcg
    11941 agcttgtcga tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt
    12001 gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc
    12061 cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc
    12121 gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata
    12181 gagttggtca agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc
    12241 cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca
    12301 gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat
    12361 gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac
    12421 gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agatcatcga gagcctgcgc
    12481 gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc
    12541 aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg
    12601 gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac
    12661 cggttgtaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtga
    12721 acggcgggag atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg
    12781 aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc
    12841 ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg
    12901 agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag
    12961 aaacttctcg acagacgtcg cggtgagttc aggctttttc atatctcatt gccccccgga
    13021 tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct
    13081 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
    13141 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
    13201 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
    13261 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
    13321 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
    13381 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
    13441 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc
    13501 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
    13561 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
    13621 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
    13681 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
    13741 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
    13801 tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc
    13861 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    13921 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    13981 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    14041 cagctatgac atgattac
//
