LOCUS       sequence-494137-       20845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and 
            phleomycin selection marker. The CBE contains a ssDNA-DBD from the 
            H. sapiens RAD51 protein. This plasmid is transfected as episome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20845)
  AUTHORS   Herrmann May N, Cao A, Schmid A, Link F, Arias-Del-Angel J, Meiser 
            E, Beneke T
  TITLE     Improved base editing and functional screening in Leishmania via 
            co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, 
            and a cytosine base editor.
  JOURNAL   eLife. 2025 Feb 24;13:RP97437. doi: 10.7554/eLife.97437.
  PUBMED    39991929
REFERENCE   2  (bases 1 to 20845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2025-02-24"; volume: "13:RP97437. doi"; pages: " 
            10.7554/eLife.97437"
COMMENT     Sequence Label: pTB107-Phleo
FEATURES             Location/Qualifiers
     source          1..20845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      171..759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     660..679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1800..1820
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1821..2504
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             join(3039..3062,3063..3065,3066..7139)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3039..7139
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7170..7418
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7449..7697
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7710..7757
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7773..7790
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8692..8712
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8737..12654
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQRPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFLFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      12374..12383
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12655..12681
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             12691..12717
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             12727..12753
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             12763..12789
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             12799..12825
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             12835..12861
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             12871..12936
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             12946..13002
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             13045..13065
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             17070..17444
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(18613..18629)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18613..18629)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(18626..18648)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    18637..18653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(18661..18667,18668..18685,18686..18691))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      19143..19598
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(19230..19249)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     19440..19461
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(19615..19634)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     19734..19756
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(19794..19812)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        19880..19984
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(19985..20053,20054..20845)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(20203..20222)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
       61 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      121 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      181 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      241 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      301 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      361 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      421 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      481 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      541 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      601 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      721 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      781 ttacggttcc tggccttttg ctggcctttt gctcacatgt aagcttttaa ttaaggtacc
      841 caaagaaacg gcgcttcctc gtccgcatgt gtttgcggga catgacgccg cgccactgcc
      901 acgccttttt cttgcctgct gtgaggagcg gccgcggccc ttggcgctcc cccccccccc
      961 cgcctgcgtc tccgccagcg gcggccacct gtgtcacacc ccatctcccc cgctctctct
     1021 cccccgtctc tcctggtctc gaaacaaccc gacattcgga aatatgtttg cacggcgttg
     1081 cgcgcgtgtc acacacgcac acacacaccg accagtaacc gtcccccaac accccctcct
     1141 ccgcacaaag catacccttt cgtttcgcct ttcgccactc tgccatcctc gagaccgaca
     1201 agaccagaat agcgcttatg tgtgtgtgtt tgtgtgtggg tgtgtgtgcc catgtgtgac
     1261 aaagaggcca gtgctttctc taaagcaaga gcgcgaggtt gctggctaag gactctaagt
     1321 ccctcacagt gatggcgtgt tgcattttct aaaccgtcaa tccaacacgt cggtagcata
     1381 tcggttttgt aggctcttct ggataccttc cccagtaaga ccgccactgc ttcgtgactg
     1441 aagcactgta aatatcactg aaaccgtcgc gttgctttgt ctaattatca ccgcctttgc
     1501 cagctatcgc ctcaggctgc gctctttgac taatccactc accacttctc tgccttcttc
     1561 ctccggttgt tgttgtgtcc tgcgtgtaca tggcgcgtgt ccttttcgag caaacagctg
     1621 tcttttcttt cacgataaca cactcatatt aaacgcgagt attactcatc agtcaacgtc
     1681 acattccgct ctgtccactt cgaccttaca cctctacttg tccaactatc ttccacttgt
     1741 caagcactag taccggtgcc accatgaaac ggacagccga cggaagcgag ttcgagtcac
     1801 caaagaagaa gcggaaagtc tcctcagaga ctgggcctgt cgccgtcgat ccaaccctgc
     1861 gccgccggat tgaacctcac gagtttgaag tgttctttga cccccgggag ctgagaaagg
     1921 agacatgcct gctgtacgag atcaactggg gaggcaggca ctccatctgg aggcacacct
     1981 ctcagaacac aaataagcac gtggaggtga acttcatcga gaagtttacc acagagcggt
     2041 acttctgccc caataccaga tgtagcatca catggtttct gagctggtcc ccttgcggag
     2101 agtgtagcag ggccatcacc gagttcctgt ccagatatcc acacgtgaca ctgtttatct
     2161 acatcgccag gctgtatcac cacgcagacc caaggaatag gcagggcctg cgcgatctga
     2221 tcagctccgg cgtgaccatc cagatcatga cagagcagga gtccggctac tgctggcgga
     2281 acttcgtgaa ttattctcct agcaacgagg cccactggcc taggtaccca cacctgtggg
     2341 tgcgcctgta cgtgctggag ctgtattgca tcatcctggg cctgccccct tgtctgaata
     2401 tcctgcggag aaagcagccc cagctgacct tctttacaat cgccctgcag tcttgtcact
     2461 atcagaggct gccaccccac atcctgtggg ccacaggcct gaagagcgga ggatctagcg
     2521 gaggatcaag cggaagcgag actcctggaa ccagcgaaag cgcaacccca gaaagcagcg
     2581 gaggaagtag cggaggaagc atggcaatgc agatgcagct tgaagcaaat gcagatactt
     2641 cagtggaaga agaaagcttt ggcccacaac ccatttcacg gttagagcag tgtggcataa
     2701 atgccaacga tgtgaagaaa ttggaagaag ctggattcca tactgtggag gctgttgcct
     2761 atgcgccaaa gaaggagcta ataaatatta agggaattag tgaagccaaa gctgataaaa
     2821 ttctggctga ggcagctaaa ttagttccaa tgggtttcac cactgcaact gaattccacc
     2881 aaaggcggtc agagatcata cagattacta ctggctccaa agagcttgac aaactacttc
     2941 aatctggcgg atctagcggt ggatctagcg gctctgagac ccctggaaca tccgaatccg
     3001 ccactccaga gagcagcgga ggctcttctg gaggatcaga caagaagtac agcatcggcc
     3061 tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca
     3121 gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg
     3181 gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca
     3241 gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg
     3301 agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag
     3361 aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc
     3421 acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg
     3481 ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc
     3541 tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg
     3601 tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca
     3661 aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc
     3721 tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga
     3781 cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg
     3841 acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc
     3901 tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga
     3961 acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc
     4021 accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag
     4081 agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc
     4141 aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac
     4201 tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca
     4261 gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt
     4321 tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc
     4381 cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga
     4441 gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc
     4501 agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc
     4561 ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat
     4621 acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg
     4681 tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact
     4741 tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg
     4801 cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca
     4861 atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca
     4921 gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga
     4981 agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg
     5041 gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg
     5101 ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc
     5161 aaaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg
     5221 gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga
     5281 aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga
     5341 ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca
     5401 aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg
     5461 agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg
     5521 acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg
     5581 acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca
     5641 acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg
     5701 ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga
     5761 gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa
     5821 agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc
     5881 tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg
     5941 atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc
     6001 tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg
     6061 tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa
     6121 tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg
     6181 agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa
     6241 ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca
     6301 tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt
     6361 ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta
     6421 agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag
     6481 tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca
     6541 tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag
     6601 aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg
     6661 gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc
     6721 cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc
     6781 ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga
     6841 tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca
     6901 aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata
     6961 tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca
     7021 ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc
     7081 accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggtgaca
     7141 gcggcgggag cggcgggagc ggggggagca ctaatctgag cgacatcatt gagaaggaga
     7201 ctgggaaaca gctggtcatt caggagtcca tcctgatgct gcctgaggag gtggaggaag
     7261 tgatcggcaa caagccagag tctgacatcc tggtgcacac cgcctacgac gagtccacag
     7321 atgagaatgt gatgctgctg acctctgacg cccccgagta taagccttgg gccctggtca
     7381 tccaggattc taacggcgag aataagatca agatgctgag cggaggatcc ggaggatctg
     7441 gaggcagcac caacctgtct gacatcatcg agaaggagac aggcaagcag ctggtcatcc
     7501 aggagagcat cctgatgctg cccgaagaag tcgaagaagt gatcggaaac aagcctgaga
     7561 gcgatatcct ggtccatacc gcctacgacg agagtaccga cgaaaatgtg atgctgctga
     7621 catccgacgc cccagagtat aagccctggg ctctggtcat ccaggattcc aacggagaga
     7681 acaaaatcaa aatgctgtct ggcggctcaa aaaggccggc ggccacgaaa aaggccggcc
     7741 aggcaaaaaa gaaaaagtaa gaattcctcg agcaccacca ccaccaccac tgagaattct
     7801 gtattacgcc gttttaagag ctccttcgac gcctttttgt ccttcagctg ctccacggtg
     7861 actcgcttct ctctctttcc acagtgtctc ttttttcttt tcactctcta tacaaatgtg
     7921 agcgacctcc ttttctgtac aacggccttc cggcgtgtgc ttttctcatc gacctctcct
     7981 cgcttcttgg gcactccttc atcgagcaaa caagacgagg gaggtgacgc gattggtgag
     8041 accactacga gtatggacgg agctgtctga taccccctgt tttttatctt gtaccccgct
     8101 ctgcagtgac ggtagcccgc tgctgtgctt cggtatcgcc gcttcatacg cttctctctt
     8161 ttttcaacgt gcggcgctga ttcaaaggtt acctcaacgc gacccgccgc tgcatccttt
     8221 gttgctcctc ttgtcgcaaa caaacaaaaa acgtgctgtg ctttttcctt tatcgtgtct
     8281 ttttgcaaag tctagacgac acacgaagcg tgtgatgaat gaaaaggagg agacactcga
     8341 cacttgaata tttctcccta tcaagccctt tcaggcctgc gtgctccctc aacccctttc
     8401 tcgataacgt gcgacgtaat gttacatgca cacacgtcgc acatctcctg tgaatggcac
     8461 tgccttctcg tgccgtcacc atgcccgcct ttcagcactg gctcttcgtt tcttcaccgc
     8521 ttcttcgttc tgtgaacccg cgcgcacgca cgcatcgaag ggattcatcg ccattgccct
     8581 ctccgcctct ctcgctcgct gcacattgac tctccacagc cccccctccc tcgcacagcc
     8641 agccccaccc acccacccac cgctctacac acctccacag cagccatggc cccaaagaag
     8701 aagcggaagg tcggtatcca cggagtccca gcagccacac agttcgaggg ctttaccaac
     8761 ctgtatcagg tgagcaagac actgcggttt gagctgatcc cacagggcaa gaccctgaag
     8821 cacatccagg agcagggctt catcgaggag gacaaggccc gcaatgatca ctacaaggag
     8881 ctgaagccca tcatcgatcg gatctacaag acctatgccg accagtgcct gcagctggtg
     8941 cagctggatt gggagaacct gagcgccgcc atcgactcct atagaaagga gaaaaccgag
     9001 gagacaagga acgccctgat cgaggagcag gccacatatc gcaatgccat ccacgactac
     9061 ttcatcggcc ggacagacaa cctgaccgat gccatcaata agagacacgc cgagatctac
     9121 aagggcctgt tcaaggccga gctgtttaat ggcaaggtgc tgaagcagct gggcaccgtg
     9181 accacaaccg agcacgagaa cgccctgctg cggagcttcg acaagtttac aacctacttc
     9241 tccggctttt atgagaacag gaagaacgtg ttcagcgccg aggatatcag cacagccatc
     9301 ccacaccgca tcgtgcagga caacttcccc aagtttaagg agaattgtca catcttcaca
     9361 cgcctgatca ccgccgtgcc cagcctgcgg gagcactttg agaacgtgaa gaaggccatc
     9421 ggcatcttcg tgagcacctc catcgaggag gtgttttcct tcccttttta taaccagctg
     9481 ctgacacaga cccagatcga cctgtataac cagctgctgg gaggaatctc tcgggaggca
     9541 ggcaccgaga agatcaaggg cctgaacgag gtgctgaatc tggccatcca gaagaatgat
     9601 gagacagccc acatcatcgc ctccctgcca cacagattca tccccctgtt taagcagatc
     9661 ctgtccgata ggaacaccct gtctttcatc ctggaggagt ttaagagcga cgaggaagtg
     9721 atccagtcct tctgcaagta caagacactg ctgagaaacg agaacgtgct ggagacagcc
     9781 gaggccctgt ttaacgagct gaacagcatc gacctgacac acatcttcat cagccacaag
     9841 aagctggaga caatcagcag cgccctgtgc gaccactggg atacactgag gaatgccctg
     9901 tatgagcgga gaatctccga gctgacaggc aagatcacca agtctgccaa ggagaaggtg
     9961 cagcgcagcc tgaagcacga ggatatcaac ctgcaggaga tcatctctgc cgcaggcaag
    10021 gagctgagcg aggccttcaa gcagaaaacc agcgagatcc tgtcccacgc acacgccgcc
    10081 ctggatcagc cactgcctac aaccctgaag aagcaggagg agaaggagat cctgaagtct
    10141 cagctggaca gcctgctggg cctgtaccac ctgctggact ggtttgccgt ggatgagtcc
    10201 aacgaggtgg accccgagtt ctctgcccgg ctgaccggca tcaagctgga gatggagcct
    10261 tctctgagct tctacaacaa ggccagaaat tatgccacca agaagcccta ctccgtggag
    10321 aagttcaagc tgaactttca gaggcctaca ctggcctctg gctgggacgt gaataaggag
    10381 aagaacaatg gcgccatcct gtttgtgaag aacggcctgt actatctggg catcatgcca
    10441 aagcagaagg gcaggtataa ggccctgagc ttcgagccca cagagaaaac cagcgagggc
    10501 tttgataaga tgtactatga ctacttccct gatgccgcca agatgatccc aaagtgcagc
    10561 acccagctga aggccgtgac agcccacttt cagacccaca caacccccat cctgctgtcc
    10621 aacaatttca tcgagcctct ggagatcaca aaggagatct acgacctgaa caatcctgag
    10681 aaggagccaa agaagtttca gacagcctac gccaagaaaa ccggcgacca gaagggctac
    10741 agagaggccc tgtgcaagtg gatcgacttc acaagggatt ttctgtccaa gtataccaag
    10801 acaacctcta tcgatctgtc tagcctgcgg ccatcctctc agtataagga cctgggcgag
    10861 tactatgccg agctgaatcc cctgctgtac cacatcagct tccagagaat cgccgagaag
    10921 gagatcatgg atgccgtgga gacaggcaag ctgtacctgt tccagatcta taacaaggac
    10981 tttgccaagg gccaccacgg caagcctaat ctgcacacac tgtattggac cggcctgttt
    11041 tctccagaga acctggccaa gacaagcatc aagctgaatg gccaggccga gctgttctac
    11101 cgccctaagt ccaggatgaa gaggatggca caccggctgg gagagaagat gctgaacaag
    11161 aagctgaagg atcagaaaac cccaatcccc gacaccctgt accaggagct gtacgactat
    11221 gtgaatcaca gactgtccca cgacctgtct gatgaggcca gggccctgct gcccaacgtg
    11281 atcaccaagg aggtgtctca cgagatcatc aaggataggc gctttaccag cgacaagttc
    11341 ttgttccacg tgcctatcac actgaactat caggccgcca attccccatc taagttcaac
    11401 cagagggtga atgcctacct gaaggagcac cccgagacac ctatcatcgg catcgatcgg
    11461 ggcgagagaa acctgatcta tatcacagtg atcgactcca ccggcaagat cctggagcag
    11521 cggagcctga acaccatcca gcagtttgat taccagaaga agctggacaa cagggagaag
    11581 gagagggtgg cagcaaggca ggcctggtct gtggtgggca caatcaagga tctgaagcag
    11641 ggctatctga gccaggtcat ccacgagatc gtggacctga tgatccacta ccaggccgtg
    11701 gtggtgctgg agaacctgaa tttcggcttt aagagcaaga ggaccggcat cgccgagaag
    11761 gccgtgtacc agcagttcga gaagatgctg atcgataagc tgaattgcct ggtgctgaag
    11821 gactatccag cagagaaagt gggaggcgtg ctgaacccat accagctgac agaccagttc
    11881 acctcctttg ccaagatggg cacccagtct ggcttcctgt tttacgtgcc tgccccatat
    11941 acatctaaga tcgatcccct gaccggcttc gtggacccct tcgtgtggaa aaccatcaag
    12001 aatcacgaga gccgcaagca cttcctggag ggcttcgact ttctgcacta cgacgtgaaa
    12061 accggcgact tcatcctgca ctttaagatg aacagaaatc tgtccttcca gaggggcctg
    12121 cccggcttta tgcctgcatg ggatatcgtg ttcgagaaga acgagacaca gtttgacgcc
    12181 aagggcaccc ctttcatcgc cggcaagaga atcgtgccag tgatcgagaa tcacagattc
    12241 accggcagat accgggacct gtatcctgcc aacgagctga tcgccctgct ggaggagaag
    12301 ggcatcgtgt tcagggatgg ctccaacatc ctgccaaagc tgctggagaa tgacgattct
    12361 cacgccatcg acaccatggt ggccctgatc cgcagcgtgc tgcagatgcg gaactccaat
    12421 gccgccacag gcgaggacta tatcaacagc cccgtgcgcg atctgaatgg cgtgtgcttc
    12481 gactcccggt ttcagaaccc agagtggccc atggacgccg atgccaatgg cgcctaccac
    12541 atcgccctga agggccagct gctgctgaat cacctgaagg agagcaagga tctgaagctg
    12601 cagaacggca tctccaatca ggactggctg gcctacatcc aggagctgcg caaccctgct
    12661 gctaaacgtg ttaagcttga tgggggtagc ccggcagcca agagagtcaa actcgacggc
    12721 ggctccccag cggcaaaaag ggtgaaacta gacgggggta gccccgccgc gaagcgtgta
    12781 aagctggatg gaggatcgcc tgcggctaag cgagtcaaat tagacggtgg gtcaccggct
    12841 gcaaagcgcg tcaaattgga tggcagtgga gactataagg accacgacgg agactacaag
    12901 gatcatgata ttgattacaa agacgatgac gataagggat ccggcgcaac aaacttctct
    12961 ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggagac gattaacatc
    13021 gctaagaacg aattcctcga gcctccaaaa aagaagagaa aggtcgaatt ctctgacatc
    13081 gaactggctg ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc
    13141 gaacagttgg cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg
    13201 tttgagcgtc aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc
    13261 actaccctac tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct
    13321 aagcgcggca agcgcccgac agccttccag ttcctgcaag aaatcaagcc ggaagccgta
    13381 gcgtacatca ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt
    13441 caggctgtag caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc
    13501 cgtgaccttg aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta
    13561 gggcacgtct acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt
    13621 ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta
    13681 cgctgcatcg agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct
    13741 ggcgtagtag gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc
    13801 gcaacccgtg caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct
    13861 cctaagccgt ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg
    13921 gcgctggtgc gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct
    13981 gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc
    14041 ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg
    14101 attgagcgtg aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc
    14161 accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc
    14221 cgtatcagcc ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc
    14281 tggttccctt acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg
    14341 caaggtaacg atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag
    14401 gaaggttact actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg
    14461 ttccctgagc gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag
    14521 tctccactgg agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc
    14581 tgctttgagt acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg
    14641 gcgtttgacg ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta
    14701 ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt
    14761 gctaagaaag tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta
    14821 gttaccgtga ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag
    14881 gcactggctg gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc
    14941 atgacgctgg cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc
    15001 attcagccag ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct
    15061 ggatacatgg ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa
    15121 gcaatgaact ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag
    15181 actggagaga ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct
    15241 gtgtggcagg aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag
    15301 ttccgcttac agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag
    15361 gagtctggta tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact
    15421 gtagtgtggg cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc
    15481 ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac
    15541 acatatgagt cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac
    15601 gagtctcaat tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac
    15661 atcttagagt cggacttcgc gttcgcgtag gcggccgccc tcctcctcct ttcttgttcc
    15721 tttcacgtcg ccttctcggt tgtagctggc agacgacgag tcttactttt acgtgtactt
    15781 ctctatagat gatgtatgat ctctctgcat gcgtgttcgt gcatgtgtcc gtgtgttgtg
    15841 tacgcgtgcg tctcgcctca gctctccgcg tgaaagggtt tgactgccca tgatgcgtgt
    15901 gtatatccac gcgcaggcac gcacacacac acacacacac acacacacag gcacacacag
    15961 gcacacaaac gcatctcagg ccgagccgca tacgtctctc gcacggtctc gtttatttga
    16021 tcatgtagtt gagtattaaa ttgggaagac aaaaacataa tagcacgaag agtcgggcac
    16081 gaaaagcccg atctctctct ctctctctct cgtgcgcgca ggggcgtgtg ggtgcacgac
    16141 gacgaggacg gaggggggaa gggaggcata aacggatccc ccatcgcaca tgcgggtacg
    16201 agcattatct gctgtgtctg gtcctttatc atatcgctac ccgcccgccc cccgcccccc
    16261 yccccccccc ccccccgcgg ctcctgtcac tgtcgttcct gcgactcccc acacacccgc
    16321 gcaacgctca tgtcacgaag agaaaagtag aaggcgtggc gatgcgttgc gcggccccct
    16381 gtccgctggc atgcagacgt cgagccgtgg aacggatggt agcgagagac aacgcggcga
    16441 tgcaggaaag gagatttact gcaggacgtc tacacacgcg cgcacaccac tgcgaatggc
    16501 accgtgctga ggaaaattgg gggggagggc gcacgggggc ggggcaggaa acggttggat
    16561 cagcagcact ctcaacttgt gtccgtattg cgaaggaggg agaggagcct gcccgtatta
    16621 tgggcgctga aacgtcggaa ccacctatga atcccacttc tgcgtcgcgg aggcgtactg
    16681 cttccgacca cctccgcagc tcacgcgcgt cgagctccct ccctttcccc ttctttccgt
    16741 tgtgtgtgtg tgtgtgtgtg tgtcttgtca cgatgcatgg ccatctctcc tccaaccctc
    16801 cacgccttcc tctcccccgc ccatcaaacg cgctacggcc acaaccattt cttcaagtat
    16861 cacatccacc accactctta cccacctacc ctcttcgact ctgacacagc cactgacgcc
    16921 ctctccgcct ctctcgctcg ctgcacattg actctccaca gccccccctc cctcgcacag
    16981 ccagccccac ccacccaccc acccacccac cgctctacac acctccacag cagccagatc
    17041 ctagtatgaa gatttcggtg atcgtcgaca tggccaagtt gaccagtgcc gttccggtgc
    17101 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
    17161 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
    17221 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
    17281 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
    17341 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
    17401 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgaggagga tcccgtatct
    17461 cgaggatcta gagatccagc aggcggagaa agagggaagt ataaggcgga cgcataggtc
    17521 ggagtatcga gaaaaagagg cagagatggg tggtggcgga gcgcccctct ctgcccttct
    17581 ctgttttact gttcccgcca cgtcgccaac tcctcttttt gtttgctcta atgtcggtgc
    17641 tatcctcttc ctctttttac tctccgtttt tcttccttcc gttttgtctt gttatcaccg
    17701 ttatttttct tcttctttct tagccgattt gggtctcctg cctacggcag cggtgatgag
    17761 tcgcacttcc gtcccccttt cctctccgta agtactccct cgatgcctca ggcgcttcta
    17821 ttttgcggca ctgtgctgac cacctcccac gtgtgcagtg agagcgccag agacattcag
    17881 gagaagaggg aagaggggaa gtaaatacca aagcgaggaa gatgtctttc tcgctgcttc
    17941 tcatcctgtt gaccggtgtg cacggcggtg tgtcctctcg gcttccctcc tccttccctc
    18001 gctccccttt ttctgtgttt tttctttcta acatgattgc gcctgctctt tttgccccgc
    18061 agcgtcgcag tgggtgtcac ctgcgaccgc cgtacgtttt actatataca tatttgtttc
    18121 tgtgctgatg ccatttgtcc gctactccat atcgtgcgtg tttgttttcc agtggtgacg
    18181 ccttccgcat cattttaatc aattcgaggc gaaaagggtg atttgagtgt tggagtgggc
    18241 ttttttattt atttgactcc aaacatcttt tcttttcccg gtatcttcgg gtccgtcacg
    18301 caggtcggtg ggtgtgtccg agtgcgcttc tttactcacg tgccgctgcg cacatacaca
    18361 gattttaaaa gcacgcatac acgatctgtg ccttcaaaac taatcaacaa aaacaaaaat
    18421 acaaaaaaga caagaataag aggtcagcaa cgcacccacg gctcctttct tcctgatcca
    18481 cgtcgtggcc gctgtacgct ctcaaacacg ctttggcgct gatgcgtgcc tttatacaaa
    18541 gaacaaagaa gaagcgaaaa acgggagcgt gttgctttgg ggatgtatgc gtgccacagt
    18601 tctgcgaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    18661 caacatacga gccggaagca taaagtgtaa atgcatgcgg ccgcacagtg tgtgggtgag
    18721 gtgcgcgaag gtgtgtctgt cggtggggga gctcgcgcgg tgtgtgcgtt ggcggctcag
    18781 cggcccccct cgcgtctctc acgccggccc gcctgtcccc ctcccccaca taaaaggacg
    18841 cagcgcgctc agcttttgtg tgcatgtatg ctatcgctgg tcgcgtgtgc tgcgcctctg
    18901 tatcttcttt tgaatctttc cctttttttc gctttctttt atcttcctcc cgtgtaccgc
    18961 agttgatgga tcaggcgccc tgttgcaact tgggccgccg acggtgctgg tgcgaggcag
    19021 caggccagca tgttgccgag atcttaatta aaatccctag cctgcagggg cgcctgatgc
    19081 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
    19141 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    19201 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    19261 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    19321 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
    19381 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    19441 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
    19501 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
    19561 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
    19621 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
    19681 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
    19741 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
    19801 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
    19861 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    19921 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    19981 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    20041 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    20101 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    20161 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    20221 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    20281 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    20341 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    20401 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    20461 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    20521 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    20581 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    20641 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    20701 aagccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    20761 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    20821 tgcctcactg attaagcatt ggtaa
//
