LOCUS       sequence-494138-       21609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and 
            hygromycin selection marker. The CBE contains a ssDNA-DBD from the 
            L. major RAD51 protein. This plasmid is transfected as episome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21609)
  AUTHORS   Herrmann May N, Cao A, Schmid A, Link F, Arias-Del-Angel J, Meiser 
            E, Beneke T
  TITLE     Improved base editing and functional screening in Leishmania via 
            co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, 
            and a cytosine base editor.
  JOURNAL   eLife. 2025 Feb 24;13:RP97437. doi: 10.7554/eLife.97437.
  PUBMED    39991929
REFERENCE   2  (bases 1 to 21609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2025-02-24"; volume: "13:RP97437. doi"; pages: " 
            10.7554/eLife.97437"
COMMENT     Sequence Label: pTB106-Hyg
FEATURES             Location/Qualifiers
     source          1..21609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      171..759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     660..679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1799..1819
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1820..2503
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             join(3149..3172,3173..3175,3176..7249)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3149..7249
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7280..7528
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7559..7807
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7820..7867
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7883..7900
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8802..8822
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8847..12764
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQRPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFLFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      12484..12493
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12765..12791
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             12801..12827
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             12837..12863
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             12873..12899
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             12909..12935
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             12945..12971
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             12981..13046
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             13056..13112
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             13155..13175
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             17184..18209
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             17184..18209
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(19377..19393)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19377..19393)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(19390..19412)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    19401..19417
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(19425..19431,19432..19449,19450..19455))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      19907..20362
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(19994..20013)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     20204..20225
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(20379..20398)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     20498..20520
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(20558..20576)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        20644..20748
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(20749..20817,20818..21609)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(20967..20986)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
       61 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      121 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      181 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      241 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      301 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      361 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      421 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      481 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      541 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      601 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      721 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      781 ttacggttcc tggccttttg ctggcctttt gctcacatgt aagcttttaa ttaaggtacc
      841 caaagaaacg gcgcttcctc gtccgcatgt gtttgcggga catgacgccg cgccactgcc
      901 acgccttttt cttgcctgct gtgaggagcg gccgcggccc ttggcgcycc cccccccccc
      961 gcctgcgtct ccgccagcgg cggccacctg tgtcacaccc catctccccc gctctctctc
     1021 ccccgtctct cctggtctcg aaacaacccg acattcggaa atatgtttgc acggcgttgc
     1081 gcgcgtgtca cacacgcaca cacacaccga ccagtaaccg tcccccaaca ccccctcctc
     1141 cgcacaaagc ataccctttc gtttcgcctt tcgccactct gccatcctcg agaccgacaa
     1201 gaccagaata gcgcttatgt gtgtgtgttt gtgtgtgggt gtgtgtgccc atgtgtgaca
     1261 aagaggccag tgctttctct aaagcaagag cgcgaggttg ctggctaagg actctaagtc
     1321 cctcacagtg atggcgtgtt gcattttcta aaccgtcaat ccaacacgtc ggtagcatat
     1381 cggttttgta ggctcttctg gataccttcc ccagtaagac cgccactgct tcgtgactga
     1441 agcactgtaa atatcactga aaccgtcgcg ttgctttgtc taattatcac cgcctttgcc
     1501 agctatcgcc tcaggctgcg ctctttgact aatccactca ccacttctct gccttcttcc
     1561 tccggttgtt gttgtgtcct gcgtgtacat ggcgcgtgtc cttttcgagc aaacagctgt
     1621 cttttctttc acgataacac actcatatta aacgcgagta ttactcatca gtcaacgtca
     1681 cattccgctc tgtccacttc gaccttacac ctctacttgt ccaactatct tccacttgtc
     1741 aagcactagt accggtgcca ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc
     1801 aaagaagaag cggaaagtct cctcagagac tgggcctgtc gccgtcgatc caaccctgcg
     1861 ccgccggatt gaacctcacg agtttgaagt gttctttgac ccccgggagc tgagaaagga
     1921 gacatgcctg ctgtacgaga tcaactgggg aggcaggcac tccatctgga ggcacacctc
     1981 tcagaacaca aataagcacg tggaggtgaa cttcatcgag aagtttacca cagagcggta
     2041 cttctgcccc aataccagat gtagcatcac atggtttctg agctggtccc cttgcggaga
     2101 gtgtagcagg gccatcaccg agttcctgtc cagatatcca cacgtgacac tgtttatcta
     2161 catcgccagg ctgtatcacc acgcagaccc aaggaatagg cagggcctgc gcgatctgat
     2221 cagctccggc gtgaccatcc agatcatgac agagcaggag tccggctact gctggcggaa
     2281 cttcgtgaat tattctccta gcaacgaggc ccactggcct aggtacccac acctgtgggt
     2341 gcgcctgtac gtgctggagc tgtattgcat catcctgggc ctgccccctt gtctgaatat
     2401 cctgcggaga aagcagcccc agctgacctt ctttacaatc gccctgcagt cttgtcacta
     2461 tcagaggctg ccaccccaca tcctgtgggc cacaggcctg aagagcggag gatctagcgg
     2521 aggatcaagc ggaagcgaga ctcctggaac cagcgaaagc gcaaccccag aaagcagcgg
     2581 aggaagtagc ggaggatcca tgcagacccg ttccaaggcc aaaggtcgcc gtggtcgtcc
     2641 gtcggcgcgg ccctctgaag aggttgaggt tgtggagagc cagccgcagg aggtcctaca
     2701 gaacgaggag caggagcctc agcagcagca gcagcagcag agcaccgaca tggctgaacc
     2761 gaacgcaagt ggctttcgcg ttatccagat cttggagaac tacggcgtgg ccagctcaga
     2821 tatcaagaag ctcatggagt gcggctttta cacggtggag tcggcggcct acgcaccgaa
     2881 gaaggccatc ctggcagtga aggggatcag cgagaacaag gccgagaaaa ttatggcgga
     2941 gtgcgccaag ctggtgccga tggggttcac ttccgcggtt gcctaccacg aggcgcgcaa
     3001 ggagatcatt atggtcacta cgggcagccg cgaggtggac aagctactcg gctcgggcgg
     3061 atctagcggt ggatctagcg gctctgagac ccctggaaca tccgaatccg ccactccaga
     3121 gagcagcgga ggctcttctg gaggatcaga caagaagtac agcatcggcc tggccatcgg
     3181 caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt
     3241 caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct
     3301 gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata
     3361 caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa
     3421 ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa
     3481 gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta
     3541 ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg
     3601 gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg
     3661 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
     3721 caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct
     3781 gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga
     3841 gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt
     3901 caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga
     3961 cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc
     4021 cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat
     4081 caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct
     4141 gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt
     4201 cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt
     4261 ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa
     4321 gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca
     4381 ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt
     4441 cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt
     4501 gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac
     4561 catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat
     4621 cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag
     4681 cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga
     4741 gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct
     4801 gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat
     4861 cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg
     4921 cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa
     4981 cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat
     5041 cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa
     5101 gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga
     5161 caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa
     5221 cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca
     5281 ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc
     5341 cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg
     5401 ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa
     5461 gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg
     5521 cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta
     5581 cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg
     5641 gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat
     5701 cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc
     5761 cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat
     5821 tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga
     5881 taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc
     5941 acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga
     6001 agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt
     6061 ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
     6121 cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga
     6181 ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc
     6241 taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct
     6301 ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat
     6361 cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt
     6421 gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc
     6481 caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg
     6541 cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg
     6601 caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag
     6661 cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa
     6721 ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag
     6781 aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata
     6841 tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa
     6901 tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca
     6961 gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc
     7021 cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct
     7081 gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga
     7141 ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat
     7201 caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggtgaca gcggcgggag
     7261 cggcgggagc ggggggagca ctaatctgag cgacatcatt gagaaggaga ctgggaaaca
     7321 gctggtcatt caggagtcca tcctgatgct gcctgaggag gtggaggaag tgatcggcaa
     7381 caagccagag tctgacatcc tggtgcacac cgcctacgac gagtccacag atgagaatgt
     7441 gatgctgctg acctctgacg cccccgagta taagccttgg gccctggtca tccaggattc
     7501 taacggcgag aataagatca agatgctgag cggaggatcc ggaggatctg gaggcagcac
     7561 caacctgtct gacatcatcg agaaggagac aggcaagcag ctggtcatcc aggagagcat
     7621 cctgatgctg cccgaagaag tcgaagaagt gatcggaaac aagcctgaga gcgatatcct
     7681 ggtccatacc gcctacgacg agagtaccga cgaaaatgtg atgctgctga catccgacgc
     7741 cccagagtat aagccctggg ctctggtcat ccaggattcc aacggagaga acaaaatcaa
     7801 aatgctgtct ggcggctcaa aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa
     7861 gaaaaagtaa gaattcctcg agcaccacca ccaccaccac tgagaattct gtattacgcc
     7921 gttttaagag ctccttcgac gcctttttgt ccttcagctg ctccacggtg actcgcttct
     7981 ctctctttcc acagtgtctc ttttttcttt tcactctcta tacaaatgtg agcgacctcc
     8041 ttttctgtac aacggccttc cggcgtgtgc ttttctcatc gacctctcct cgcttcttgg
     8101 gcactccttc atcgagcaaa caagacgagg gaggtgacgc gattggtgag accactacga
     8161 gtatggacgg agctgtctga taccccctgt tttttatctt gtaccccgct ctgcagtgac
     8221 ggtagcccgc tgctgtgctt cggtatcgcc gcttcatacg cttctctctt ttttcaacgt
     8281 gcggcgctga ttcaaaggtt acctcaacgc gacccgccgc tgcatccttt gttgctcctc
     8341 ttgtcgcaaa caaacaaaaa acgtgctgtg ctttttcctt tatcgtgtct ttttgcaaag
     8401 tctagacgac acacgaagcg tgtgatgaat gaaaaggagg agacactcga cacttgaata
     8461 tttctcccta tcaagccctt tcaggcctgc gtgctccctc aacccctttc tcgataacgt
     8521 gcgacgtaat gttacatgca cacacgtcgc acatctcctg tgaatggcac tgccttctcg
     8581 tgccgtcacc atgcccgcct ttcagcactg gctcttcgtt tcttcaccgc ttcttcgttc
     8641 tgtgaacccg cgcgcacgca cgcatcgaag ggattcatcg ccattgccct ctccgcctct
     8701 ctcgctcgct gcacattgac tctccacagc cccccctccc tcgcacagcc agccccaccc
     8761 acccacccac cgctctacac acctccacag cagccatggc cccaaagaag aagcggaagg
     8821 tcggtatcca cggagtccca gcagccacac agttcgaggg ctttaccaac ctgtatcagg
     8881 tgagcaagac actgcggttt gagctgatcc cacagggcaa gaccctgaag cacatccagg
     8941 agcagggctt catcgaggag gacaaggccc gcaatgatca ctacaaggag ctgaagccca
     9001 tcatcgatcg gatctacaag acctatgccg accagtgcct gcagctggtg cagctggatt
     9061 gggagaacct gagcgccgcc atcgactcct atagaaagga gaaaaccgag gagacaagga
     9121 acgccctgat cgaggagcag gccacatatc gcaatgccat ccacgactac ttcatcggcc
     9181 ggacagacaa cctgaccgat gccatcaata agagacacgc cgagatctac aagggcctgt
     9241 tcaaggccga gctgtttaat ggcaaggtgc tgaagcagct gggcaccgtg accacaaccg
     9301 agcacgagaa cgccctgctg cggagcttcg acaagtttac aacctacttc tccggctttt
     9361 atgagaacag gaagaacgtg ttcagcgccg aggatatcag cacagccatc ccacaccgca
     9421 tcgtgcagga caacttcccc aagtttaagg agaattgtca catcttcaca cgcctgatca
     9481 ccgccgtgcc cagcctgcgg gagcactttg agaacgtgaa gaaggccatc ggcatcttcg
     9541 tgagcacctc catcgaggag gtgttttcct tcccttttta taaccagctg ctgacacaga
     9601 cccagatcga cctgtataac cagctgctgg gaggaatctc tcgggaggca ggcaccgaga
     9661 agatcaaggg cctgaacgag gtgctgaatc tggccatcca gaagaatgat gagacagccc
     9721 acatcatcgc ctccctgcca cacagattca tccccctgtt taagcagatc ctgtccgata
     9781 ggaacaccct gtctttcatc ctggaggagt ttaagagcga cgaggaagtg atccagtcct
     9841 tctgcaagta caagacactg ctgagaaacg agaacgtgct ggagacagcc gaggccctgt
     9901 ttaacgagct gaacagcatc gacctgacac acatcttcat cagccacaag aagctggaga
     9961 caatcagcag cgccctgtgc gaccactggg atacactgag gaatgccctg tatgagcgga
    10021 gaatctccga gctgacaggc aagatcacca agtctgccaa ggagaaggtg cagcgcagcc
    10081 tgaagcacga ggatatcaac ctgcaggaga tcatctctgc cgcaggcaag gagctgagcg
    10141 aggccttcaa gcagaaaacc agcgagatcc tgtcccacgc acacgccgcc ctggatcagc
    10201 cactgcctac aaccctgaag aagcaggagg agaaggagat cctgaagtct cagctggaca
    10261 gcctgctggg cctgtaccac ctgctggact ggtttgccgt ggatgagtcc aacgaggtgg
    10321 accccgagtt ctctgcccgg ctgaccggca tcaagctgga gatggagcct tctctgagct
    10381 tctacaacaa ggccagaaat tatgccacca agaagcccta ctccgtggag aagttcaagc
    10441 tgaactttca gaggcctaca ctggcctctg gctgggacgt gaataaggag aagaacaatg
    10501 gcgccatcct gtttgtgaag aacggcctgt actatctggg catcatgcca aagcagaagg
    10561 gcaggtataa ggccctgagc ttcgagccca cagagaaaac cagcgagggc tttgataaga
    10621 tgtactatga ctacttccct gatgccgcca agatgatccc aaagtgcagc acccagctga
    10681 aggccgtgac agcccacttt cagacccaca caacccccat cctgctgtcc aacaatttca
    10741 tcgagcctct ggagatcaca aaggagatct acgacctgaa caatcctgag aaggagccaa
    10801 agaagtttca gacagcctac gccaagaaaa ccggcgacca gaagggctac agagaggccc
    10861 tgtgcaagtg gatcgacttc acaagggatt ttctgtccaa gtataccaag acaacctcta
    10921 tcgatctgtc tagcctgcgg ccatcctctc agtataagga cctgggcgag tactatgccg
    10981 agctgaatcc cctgctgtac cacatcagct tccagagaat cgccgagaag gagatcatgg
    11041 atgccgtgga gacaggcaag ctgtacctgt tccagatcta taacaaggac tttgccaagg
    11101 gccaccacgg caagcctaat ctgcacacac tgtattggac cggcctgttt tctccagaga
    11161 acctggccaa gacaagcatc aagctgaatg gccaggccga gctgttctac cgccctaagt
    11221 ccaggatgaa gaggatggca caccggctgg gagagaagat gctgaacaag aagctgaagg
    11281 atcagaaaac cccaatcccc gacaccctgt accaggagct gtacgactat gtgaatcaca
    11341 gactgtccca cgacctgtct gatgaggcca gggccctgct gcccaacgtg atcaccaagg
    11401 aggtgtctca cgagatcatc aaggataggc gctttaccag cgacaagttc ttgttccacg
    11461 tgcctatcac actgaactat caggccgcca attccccatc taagttcaac cagagggtga
    11521 atgcctacct gaaggagcac cccgagacac ctatcatcgg catcgatcgg ggcgagagaa
    11581 acctgatcta tatcacagtg atcgactcca ccggcaagat cctggagcag cggagcctga
    11641 acaccatcca gcagtttgat taccagaaga agctggacaa cagggagaag gagagggtgg
    11701 cagcaaggca ggcctggtct gtggtgggca caatcaagga tctgaagcag ggctatctga
    11761 gccaggtcat ccacgagatc gtggacctga tgatccacta ccaggccgtg gtggtgctgg
    11821 agaacctgaa tttcggcttt aagagcaaga ggaccggcat cgccgagaag gccgtgtacc
    11881 agcagttcga gaagatgctg atcgataagc tgaattgcct ggtgctgaag gactatccag
    11941 cagagaaagt gggaggcgtg ctgaacccat accagctgac agaccagttc acctcctttg
    12001 ccaagatggg cacccagtct ggcttcctgt tttacgtgcc tgccccatat acatctaaga
    12061 tcgatcccct gaccggcttc gtggacccct tcgtgtggaa aaccatcaag aatcacgaga
    12121 gccgcaagca cttcctggag ggcttcgact ttctgcacta cgacgtgaaa accggcgact
    12181 tcatcctgca ctttaagatg aacagaaatc tgtccttcca gaggggcctg cccggcttta
    12241 tgcctgcatg ggatatcgtg ttcgagaaga acgagacaca gtttgacgcc aagggcaccc
    12301 ctttcatcgc cggcaagaga atcgtgccag tgatcgagaa tcacagattc accggcagat
    12361 accgggacct gtatcctgcc aacgagctga tcgccctgct ggaggagaag ggcatcgtgt
    12421 tcagggatgg ctccaacatc ctgccaaagc tgctggagaa tgacgattct cacgccatcg
    12481 acaccatggt ggccctgatc cgcagcgtgc tgcagatgcg gaactccaat gccgccacag
    12541 gcgaggacta tatcaacagc cccgtgcgcg atctgaatgg cgtgtgcttc gactcccggt
    12601 ttcagaaccc agagtggccc atggacgccg atgccaatgg cgcctaccac atcgccctga
    12661 agggccagct gctgctgaat cacctgaagg agagcaagga tctgaagctg cagaacggca
    12721 tctccaatca ggactggctg gcctacatcc aggagctgcg caaccctgct gctaaacgtg
    12781 ttaagcttga tgggggtagc ccggcagcca agagagtcaa actcgacggc ggctccccag
    12841 cggcaaaaag ggtgaaacta gacgggggta gccccgccgc gaagcgtgta aagctggatg
    12901 gaggatcgcc tgcggctaag cgagtcaaat tagacggtgg gtcaccggct gcaaagcgcg
    12961 tcaaattgga tggcagtgga gactataagg accacgacgg agactacaag gatcatgata
    13021 ttgattacaa agacgatgac gataagggat ccggcgcaac aaacttctct ctgctgaaac
    13081 aagccggaga tgtcgaagag aatcctggac cgatggagac gattaacatc gctaagaacg
    13141 aattcctcga gcctccaaaa aagaagagaa aggtcgaatt ctctgacatc gaactggctg
    13201 ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc gaacagttgg
    13261 cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc
    13321 aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc actaccctac
    13381 tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct aagcgcggca
    13441 agcgcccgac agccttccag ttcctgcaag aaatcaagcc ggaagccgta gcgtacatca
    13501 ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt caggctgtag
    13561 caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc cgtgaccttg
    13621 aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta gggcacgtct
    13681 acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt ctactcggtg
    13741 gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta cgctgcatcg
    13801 agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct ggcgtagtag
    13861 gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc gcaacccgtg
    13921 caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct cctaagccgt
    13981 ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg gcgctggtgc
    14041 gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct gaggtgtaca
    14101 aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc ctagcggtcg
    14161 ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg attgagcgtg
    14221 aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc accgcgtgga
    14281 aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc cgtatcagcc
    14341 ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc tggttccctt
    14401 acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg caaggtaacg
    14461 atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag gaaggttact
    14521 actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg ttccctgagc
    14581 gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag tctccactgg
    14641 agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc tgctttgagt
    14701 acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg gcgtttgacg
    14761 ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta ggtggtcgcg
    14821 cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt gctaagaaag
    14881 tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta gttaccgtga
    14941 ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag gcactggctg
    15001 gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc atgacgctgg
    15061 cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc attcagccag
    15121 ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct ggatacatgg
    15181 ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact
    15241 ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag actggagaga
    15301 ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct gtgtggcagg
    15361 aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag ttccgcttac
    15421 agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag gagtctggta
    15481 tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact gtagtgtggg
    15541 cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc ggtaccattc
    15601 cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac acatatgagt
    15661 cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac gagtctcaat
    15721 tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac atcttagagt
    15781 cggacttcgc gttcgcgtag gcggccgccc tcctcctcct ttcttgttcc tttcacgtcg
    15841 ccttctcggt tgtagctggc agacgacgag tcttactttt acgtgtactt ctctatagat
    15901 gatgtatgat ctctctgcat gcgtgttcgt gcatgtgtcc gtgtgttgtg tacgcgtgcg
    15961 tctcgcctca gctctccgcg tgaaagggtt tgactgccca tgatgcgtgt gtatatccac
    16021 gcgcaggcac gcacacacac acacacacac acacacacag gcacacacag gcacacaaac
    16081 gcatctcagg ccgagccgca tacgtctctc gcacggtctc gtttatttga tcatgtagtt
    16141 gagtattaaa ttgggaagac aaaaacataa tagcacgaag agtcgggcac gaaaagcccg
    16201 atctctctct ctctctctct cgtgcgcgca ggggcgtgtg ggtgcacgac gacgaggacg
    16261 gaggggggaa gggaggcata aacggatccc ccatcgcaca tgcgggtacg agcattatct
    16321 gctgtgtctg gtcctttatc atatcgctac ccgcccgccc cccgcccccc cccccccccc
    16381 cccccccgcg gctcctgtca ctgtcgttcc tgcgactccc cacacacccg cgcaacgctc
    16441 atgtcacgaa gagaaaagta gaaggcgtgg cgatgcgttg cgcggccccc tgtccgctgg
    16501 catgcagacg tcgagccgtg gaacggatgg tagcgagaga caacgcggcg atgcaggaaa
    16561 ggagatttac tgcaggacgt ctacacacgc gcgcacacca ctgcgaatgg caccgtgctg
    16621 aggaaaattg ggggggaggg cgcacggggg cggggcagga aacggttgga tcagcagcac
    16681 tctcaacttg tgtccgtatt gcgaaggagg gagaggagcc tgcccgtatt atgggcgctg
    16741 aaacgtcgga accacctatg aatcccactt ctgcgtcgcg gaggcgtact gcttccgacc
    16801 acctccgcag ctcacgcgcg tcgagctccc tccctttccc cttctttccg ttgtgtgtgt
    16861 gtgtgtgtgt gtgtcttgtc acgatgcatg gccatctctc ctccaaccct ccacgccttc
    16921 ctctcccccg cccatcaaac gcgctacggc cacaaccatt tcttcaagta tcacatccac
    16981 caccactctt acccacctac cctcttcgac tctgacacag ccactgacgc cctctccgcc
    17041 tctctcgctc gctgcacatt gactctccac agccccccct ccctcgcaca gccagcccca
    17101 cccacccacc cacccaccca ccgctctaca cacctccaca gcagccagat cctagtatga
    17161 agatttcggt gatcgtcgac gtaatgaaaa agcctgaact caccgcgacg tctgtcgaga
    17221 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
    17281 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
    17341 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
    17401 cgattccgga agtgcttgac attggggaat ttagcgagag cctgacctat tgcatctccc
    17461 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
    17521 agccggtcgc ggaggcaatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
    17581 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat tttatatgcg
    17641 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
    17701 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
    17761 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
    17821 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
    17881 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
    17941 atccggagct tgcaggatcg cctcggctcc gggcgtatat gctccgcatt ggtcttgacc
    18001 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
    18061 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
    18121 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
    18181 ccagcactcg tccgagggca aaggaatagt aggatcccgt atctcgagga tctagagatc
    18241 cagcaggcgg agaaagaggg aagtataagg cggacgcata ggtcggagta tcgagaaaaa
    18301 gaggcagaga tgggtggtgg cggagcgccc ctctctgccc ttctctgttt tactgttccc
    18361 gccacgtcgc caactcctct ttttgtttgc tctaatgtcg gtgctatcct cttcctcttt
    18421 ttactctccg tttttcttcc ttccgttttg tcttgttatc accgttattt ttcttcttct
    18481 ttcttagccg atttgggtct cctgcctacg gcagcggtga tgagtcgcac ttccgtcccc
    18541 ctttcctctc cgtaagtact ccctcgatgc ctcaggcgct tctattttgc ggcactgtgc
    18601 tgaccacctc ccacgtgtgc agtgagagcg ccagagacat tcaggagaag agggaagagg
    18661 ggaagtaaat accaaagcga ggaagatgtc tttctcgctg cttctcatcc tgttgaccgg
    18721 tgtgcacggc ggtgtgtcct ctcggcttcc ctcctccttc cctcgctccc ctttttctgt
    18781 gttttttctt tctaacatga ttgcgcctgc tctttttgcc ccgcagcgtc gcagtgggtg
    18841 tcacctgcga ccgccgtacg ttttactata tacatatttg tttctgtgct gatgccattt
    18901 gtccgctact ccatatcgtg cgtgtttgtt ttccagtggt gacgccttcc gcatcatttt
    18961 aatcaattcg aggcgaaaag ggtgatttga gtgttggagt gggctttttt atttatttga
    19021 ctccaaacat cttttctttt cccggtatct tcgggtccgt cacgcaggtc ggtgggtgtg
    19081 tccgagtgcg cttctttact cacgtgccgc tgcgcacata cacagatttt aaaagcacgc
    19141 atacacgatc tgtgccttca aaactaatca acaaaaacaa aaatacaaaa aagacaagaa
    19201 taagaggtca gcaacgcacc cacggctcct ttcttcctga tccacgtcgt ggccgctgta
    19261 cgctctcaaa cacgctttgg cgctgatgcg tgcctttata caaagaacaa agaagaagcg
    19321 aaaaacggga gcgtgttgct ttggggatgt atgcgtgcca cagttctgcg aatcatgtca
    19381 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
    19441 agcataaagt gtaaatgcat gcggccgcac agtgtgtggg tgaggtgcgc gaaggtgtgt
    19501 ctgtcggtgg gggagctcgc gcggtgtgtg cgttggcggc tcagcggccc ccctcgcgtc
    19561 tctcacgccg gcccgcctgt ccccctcccc cacataaaag gacgcagcgc gctcagcttt
    19621 tgtgtgcatg tatgctatcg ctggtcgcgt gtgctgcgcc tctgtatctt cttttgaatc
    19681 tttccctttt tttcgctttc ttttatcttc ctcccgtgta ccgcagttga tggatcaggc
    19741 gccctgttgc aacttgggcc gccgacggtg ctggtgcgag gcagcaggcc agcatgttgc
    19801 cgagatctta attaaaatcc ctagcctgca ggggcgcctg atgcggtatt ttctccttac
    19861 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
    19921 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    19981 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    20041 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    20101 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
    20161 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    20221 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
    20281 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    20341 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
    20401 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
    20461 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
    20521 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
    20581 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
    20641 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    20701 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    20761 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
    20821 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
    20881 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
    20941 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
    21001 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    21061 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
    21121 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    21181 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    21241 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
    21301 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    21361 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    21421 gctggctggt ttattgctga taaatctgga gccggtgagc gtggaagccg cggtatcatt
    21481 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    21541 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    21601 cattggtaa
//
