LOCUS       sequence-494153-       11980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11980)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 11980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-3XKD
FEATURES             Location/Qualifiers
     source          1..11980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        764..1067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1068..1271
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1222..1242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        1317..1335
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      1367..1376
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1376..1405
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1421..1450
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1469..1493
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9105..9129)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9178..9197)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9179..9197)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    9471..9592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9508..9527)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9562..9581
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9599..10054)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9736..9757)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9948..9967
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10081..10185
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10187..10544
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10187..10378
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10210..10230)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10395..10530
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10579..11373
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10579..11373
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10633..10652)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11243..11262
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11561..11580)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11605..11652
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
      721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1261 taagcagagc tggtttagtg aaccgtcaga tccgctagcg attacgccaa gctcgaaatt
     1321 aaccctcact aaagggaaca aaagctggag ctccaccgcg gtggcggccg ccatggagca
     1381 gaaactcatc tctgaagagg atctgagccc gggcggatcc gagcagaaac tcatctctga
     1441 agaggatctg ctgcaggaat tcgatatcac aagtttgtac aaaaaagcag gctccgaatt
     1501 cgcccttatg gctagtggca gctgtcaggg gtgcgaagag gacgaggaaa ctctgaagaa
     1561 gttgatagtc aggctgaaca atgtccagga aggaaaacag atagaaacgc tggtccaaat
     1621 cctggaggat ctgctggtgt tcacgtactc cgagcacgcc tccaagttat ttcaaggcaa
     1681 aaatatccat gtgcctctgt tgatcgtctt ggactcctat atgagagtcg cgagtgtgca
     1741 gcaggtgggt tggtcacttc tgtgcaaatt aatagaagtc tgtccaggta caatgcaaag
     1801 cttaatggga ccccaggatg ttggaaatga ttgggaagtc cttggtgttc accaattgat
     1861 tcttaaaatg ctaacagttc ataatgccag tgtaaacttg tcagtgattg gactgaagac
     1921 cttagatctc ctcctaactt caggtaaaat caccttgctg atattggatg aagaaagtga
     1981 tattttcatg ttaatttttg atgccatgca ctcatttcca gccaatgatg aagtccagaa
     2041 acttggatgc aaagctttac atgtgctgtt tgagagagtc tcagaggagc aactgactga
     2101 atttgttgag aacaaagatt atatgatatt gttaagtgcg ttaacaaatt ttaaagatga
     2161 agaggaaatt gtgcttcatg tgctgcattg tttacattcc ctagcgattc cttgcaataa
     2221 tgtggaagtc ctcatgagtg gcaatgtcag gtgttataat attgtggtgg aagctatgaa
     2281 agcattccct atgagtgaaa gaattcaaga agtgagttgc tgtttgctcc ataggcttac
     2341 attaggtaat tttttcaata tcctggtatt aaacgaagtc catgagtttg tggtgaaagc
     2401 tgtgcagcag tacccagaga atgcagcatt gcagatctca gcgctcagct gtttggccct
     2461 cctcactgag actattttct taaatcaaga tttagaggaa aagaatgaga atcaagagaa
     2521 tgatgatgag ggggaagaag ataaattgtt ttggctggaa gcctgttaca aagcattaac
     2581 gtggcataga aagaacaagc acgtgcagga ggccgcatgc tgggcactaa ataatctcct
     2641 tatgtaccaa aacagtttac atgagaagat tggagatgaa gatggccatt tcccagctca
     2701 tagggaagtg atgctctcca tgctgatgca ttcttcatca aaggaagttt tccaggcatc
     2761 tgcgaatgca ttgtcaactc tcttagaaca aaatgttaat ttcagaaaaa tactgttatc
     2821 aaaaggaata cacctgaatg ttttggagtt aatgcagaag catatacatt ctcccgaagt
     2881 ggctgaaagt ggctgtaaaa tgctaaatca tctttttgaa ggaagcaaca cttccctgga
     2941 tataatggca gcagtggtcc ccaaaatact aacagttatg aaacgtcatg agacatcatt
     3001 accagtgcag ctggaggcgc ttcgagctat tttacatttt atagtgcctg gcatgccaga
     3061 agaatccagg gaggatacag aatttcatca taagctaaat atggttaaaa aacagtgttt
     3121 caagaatgat attcacaaac tggtcctagc agctttgaac aggttcattg gaaatcctgg
     3181 gattcagaaa tgtggattaa aagtaatttc ttctattgta cattttcctg atgcattaga
     3241 gatgttatcc ctggaaggtg ctatggattc agtgcttcac acactgcaga tgtatccaga
     3301 tgaccaagaa attcagtgtc tgggtttaag tcttatagga tacttgatta caaagaagaa
     3361 tgtgttcata ggaactggac atctgctggc aaaaattctg gtttccagct tataccgatt
     3421 taaggatgtt gctgaaatac agactaaagg atttcagaca atcttagcaa tcctcaaatt
     3481 gtcagcatct ttttctaagc tgctggtgca tcattcattt gacttagtaa tattccatca
     3541 aatgtcttcc aatatcatgg aacaaaagga tcaacagttt ctaaacctct gttgcaagtg
     3601 ttttgcaaaa gtagctatgg atgattactt aaaaaatgtg atgctagaga gagcgtgtga
     3661 tcagaataac agcatcatgg ttgaatgctt gcttctattg ggagcagatg ccaatcaagc
     3721 aaaggaggga tcttctttaa tttgtcaggt atgtgagaaa gagagcagtc ccaaattggt
     3781 ggaactctta ctgaatagtg gatctcgtga acaagatgta cgaaaagcgt tgacgataag
     3841 cattgggaaa ggtgacagcc agatcatcag cttgctctta aggaggctgg ccctggatgt
     3901 tgccaacaat agcatttgcc ttggaggatt ttgtatagga aaagttgaac cttcttggct
     3961 tggtccttta tttccagata agacttctaa tttaaggaaa caaacaaata tagcatctac
     4021 actagcaaga atggtgatca gatatcagat gaaaagtgct gtggaagaag gaacagcctc
     4081 aggcagcgat ggaaattttt ctgaagatgt gctgtctaaa tttgatgaat ggacctttat
     4141 tcctgactct tctatggaca gtgtgtttgc tcaaagtgat gacctggata gtgaaggaag
     4201 tgaaggctca tttcttgtga aaaagaaatc taattcaatt agtgtaggag aattttaccg
     4261 agatgccgta ttacagcgtt gctcaccaaa tttgcaaaga cattccaatt ccttggggcc
     4321 catttttgat catgaagatt tactgaagcg aaaaagaaaa atattatctt cagatgattc
     4381 actcaggtca tcaaaacttc aatcccatat gaggcattca gacagcattt cttctctggc
     4441 ttctgagaga gaatatatta catcactaga cctttcagca aatgaactaa gagatattga
     4501 tgccctaagc cagaaatgct gtataagtgt tcatttggag catcttgaaa agctggagct
     4561 tcaccagaat gcactcacga gctttccaca acagctatgt gaaactctga agagtttgac
     4621 acatttggac ttgcacagta ataaatttac atcatttcct tcttatttgt tgaaaatgag
     4681 ttgtattgct aatcttgatg tctctcgaaa tgacattgga ccctcagtgg ttttagatcc
     4741 tacagtgaaa tgtccaactc tgaaacagtt taacctgtca tataaccagc tgtcttttgt
     4801 acctgagaac ctcactgatg tggtagagaa actggagcag ctcattttag aaggaaataa
     4861 aatatcaggg gtatgctccc ccttgagact gaaggaactg aagattttaa accttagtaa
     4921 gaaccacatt tcatccctat cagagaactt tcttgaggct tgtcctaaag tggagagttt
     4981 cagtgccaga atgaattttc ttgctgctat gcctttcttg cctccttcta tgacaatcct
     5041 aaaattatct cagaacaaat tttcctgtat tccagaagca attttaaatc ttccacactt
     5101 gcggtcttta gatatgagca gcaatgatat tcagtaccta ccaggtcccg cacactggaa
     5161 atctttgaac ttaagggaac tcttatttag ccataatcag atcagcatct tggacttgag
     5221 tgaaaaagca tatttatggt ctagagtaga gaaactgcat ctttctcaca ataaactgaa
     5281 agagattcct cctgagattg gctgtcttga aaatctgaca tctctggatg tcagttacaa
     5341 cttggaacta agatcctttc ccaatgaaat ggggaaatta agcaaaatat gggatcttcc
     5401 tttggatgaa ctgcatctta actttgattt taaacatata ggatgtaaag ccaaagacat
     5461 cataaggttt cttcaacagc gattaaaaaa ggctgtgcct tataaccgaa tgaaacttat
     5521 gattgtggga aatactggga gtggtaaaac caccttattg cagcaattaa tgaaaaccaa
     5581 gaaatcagat cttggaatgc aaagtgccac agttggcata gatgtgaaag actggcctat
     5641 ccaaataaga gacaaaagaa agagagatct cgtcctaaat gtgtgggatt ttgcaggtcg
     5701 tgaggaattc tatagtactc atccccattt tatgacgcag cgagcattgt accttgctgt
     5761 ctatgacctc agcaagggac aggctgaagt tgatgccatg aagccttggc tcttcaatat
     5821 aaaggctcgc gcttcttctt cccctgtgat tctcgttggc acacatttgg atgtttctga
     5881 tgagaagcaa cgcaaagcct gcatgagtaa aatcaccaag gaactcctga ataagcgagg
     5941 gttccctgcc atacgagatt accactttgt gaatgccacc gaggaatctg atgctttggc
     6001 aaaacttcgg aaaaccatca taaacgagag ccttaatttc aagatccgag atcagcttgt
     6061 tgttggacag ctgattccag actgctatgt agaacttgaa aaaatcattt tatcggagcg
     6121 taaaaatgtg ccaattgaat ttcccgtaat tgaccggaaa cgattattac aactagtgag
     6181 agaaaatcag ctgcagttag atgaaaatga gcttcctcac gcagttcact ttctaaatga
     6241 atcaggagtc cttcttcatt ttcaagaccc agcactgcag ttaagtgact tgtactttgt
     6301 ggaacccaag tggctttgta aaatcatggc acagattttg acagtgaaag tggaaggttg
     6361 tccaaaacac cctaagggaa ttatttcgcg tagagatgtg gaaaaatttc tttcaaaaaa
     6421 aaggaaattt ccaaagaact acatgtcaca gtattttaag ctcctagaaa aattccagat
     6481 tgctttgcca ataggagaag aatatttgct ggttccaagc agtttgtctg accacaggcc
     6541 tgtgatagag cttccccatt gtgagaactc tgaaattatc atccgactat atgaaatgcc
     6601 ttattttcca atgggatttt ggtcaagatt aatcaatcga ttacttgaga tttcacctta
     6661 catgctttca gggagagaac gagcacttcg cccaaacaga atgtattggc gacaaggcat
     6721 ttacttaaat tggtctcctg aagcttattg tctggtagga tctgaagtct tagacaatca
     6781 tccagagagt ttcttaaaaa ttacagttcc ttcttgtaga aaaggctgta ttcttttggg
     6841 ccaagttgtg gaccacattg attctctcat ggaagaatgg tttcctgggt tgctggagat
     6901 tgatatttgt ggtgaaggag aaactctgtt gaagaaatgg gcattatata gttttaatga
     6961 tggcgaagaa catcaaaaaa tcttacttga tgacttgatg aagaaagcag aggaaggaga
     7021 tctcttagta aatccagatc aaccaaggct caccattcca atatctcaga ttgcccctga
     7081 cttgattttg gctgacctgc ctagaaatat tatgttgaat aatgatgagt tggaatttga
     7141 acaagctcca gagtttctcc taggtgatgg cagttttgga tcagtttacc gagcagccta
     7201 tgaaggagaa gaagtggctg tggcaatttt taataaacat acatcactca ggctgttaag
     7261 acaagagctt gtggtgcttt gccacctcca ccaccccagt ttgatatctt tgctggcagc
     7321 tgggattcgt ccccggatgt tggtgatgga gttagcctcc aagggttcct tggatcgcct
     7381 gcttcagcag gacaaagcca gcctcactag aaccctacag cacaggattg cactccacgt
     7441 agctgatggt ttgagatacc tccactcagc catgattata taccgagcac tgaaacccca
     7501 caatgtgctg cttttcacac tgtatcccaa tgctgccatc attgcaaaga ttgctgcata
     7561 cggcattgct cagtactgct gtagaatggg gataaaaaca tcagagggca caccagggtt
     7621 tcgtgcgcct gaagttgcca gaggaaatgt catttataac caacaggctg atgtttattc
     7681 atttggttta ctactctatg acattttgac aactggaggt agaatagtag agggtttgaa
     7741 gtttccaaat gagtttgatg aattagaaat acaaggaaaa ttacctgatc cagttaaaga
     7801 atatggttgt gccccatggc ctatggttga gaaattaatt aaacagtgtt tgaaagaaaa
     7861 tcctcaagaa aggcctactt ctgcccaggt ctttgacatt ttgaattcag ctgaattagt
     7921 ctgtctgacg agacgcattt tattacctaa aaacgtaatt gttgaatgca tggttgctac
     7981 acatcacaac agcaggaatg caagcatttg gctgggctgt gggcacaccg acagaggaca
     8041 gctctcattt cttgacttaa atactgaagg atacacttct gaggaagttg ctgatagtag
     8101 aatattgtgc ttagccttgg tgcatcttcc tgttgaaaag gaaagctgga ttgtgtctgg
     8161 gacacagtct ggtactctcc tggtcatcaa taccgaagat gggaaaaaga gacataccct
     8221 agaaaagatg actgattctg tcacttgttt gtattgcaat tccttttcca agcaaagcaa
     8281 acaaaaaaat tttcttttgg ttggaaccgc tgatggcaag ttagcaattt ttgaagataa
     8341 gactgttaag cttaaaggag ctgctccttt gaagatacta aatataggaa atgtcagtac
     8401 tccattgatg tgtttgagtg aatccacaaa ttcaacggaa agaaatgtaa tgtggggagg
     8461 atgtggcaca aagattttct ccttttctaa tgatttcacc attcagaaac tcattgagac
     8521 aagaacaagc caactgtttt cttatgcagc tttcagtgat tccaacatca taacagtggt
     8581 ggtagacact gctctctata ttgctaagca aaatagccct gttgtggaag tgtgggataa
     8641 gaaaactgaa aaactctgtg gactaataga ctgcgtgcac tttttaaggg aggtaatggt
     8701 aaaagaaaac aaggaatcaa aacacaaaat gtcttattct gggagagtga aaaccctctg
     8761 ccttcagaag aacactgctc tttggatagg aactggagga ggccatattt tactcctgga
     8821 tctttcaact cgtcgactta tacgtgtaat ttacaacttt tgtaattcgg tcagagtcat
     8881 gatgacagca cagctaggaa gccttaaaaa tgtcatgctg gtattgggct acaaccggaa
     8941 aaatactgaa ggtacacaaa agcagaaaga gatacaatct tgcttgaccg tttgggacat
     9001 caatcttcca catgaagtgc aaaatttaga aaaacacatt gaagtgagaa aagaattagc
     9061 tgaaaaaatg agacgaacat ctgttgagaa aaagggcgaa ttcgacccag ctttcttgta
     9121 caaagtggtg atcggtacct taattaatta aggtaccagg taagtgtacc caattcgccc
     9181 tatagtgagt cgtattacaa ttcactcgat cgcccttccc aacagttgcg cagcctgaat
     9241 ggcgaatgga gatccaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     9301 gtgtatttta gattcacagt cccaaggctc atttcaggcc cctcagtcct cacagtctgt
     9361 tcatgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     9421 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     9481 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9541 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa
     9601 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     9661 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     9721 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     9781 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     9841 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     9901 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     9961 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
    10021 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
    10081 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
    10141 caataaccct gataaatgct tcaataatat tgaaaaagga agaatcctga ggcggaaaga
    10201 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
    10261 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    10321 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    10381 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    10441 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    10501 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
    10561 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
    10621 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
    10681 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
    10741 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
    10801 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
    10861 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
    10921 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
    10981 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
    11041 caggatgatc tggacgaaga acatcagggg ctcgcgccag ccgaactgtt cgccaggctc
    11101 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
    11161 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
    11221 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga acttggcggc
    11281 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
    11341 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
    11401 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
    11461 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
    11521 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
    11581 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
    11641 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
    11701 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
    11761 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
    11821 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    11881 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    11941 actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
