LOCUS       sequence-494283-       19447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Dadh (Delta-Elen_0475) in 
            Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19447)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 19447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF7.5-Dadh-Delta-Elen0475(ElA2)
FEATURES             Location/Qualifiers
     source          1..19447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(3005..3028)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      13071..13098
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      13230..13257
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(13689..14540)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(14527..15366)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(15877..16848)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            18087..18174
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      18516..18910
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(19073..19116)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            19212..19321
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttag
     1501 tacatcagga ccactgggta gcccagctgg tagatcagca cgcgaaacat cacggaggtg
     1561 gccagcgcgc cgatggcggc gacgaccatg agcgtcttgg aggcgcccac cttcttcttc
     1621 gccacgaagg cgcaggccgc agccacgatc acggagacga tggagcccca gaacatgggc
     1681 gctcccgcgc cggacaccat gacggtcatc aggctgtcga cgtgcgtggg ggccgtggtc
     1741 atcgatgtcg ggtcggcgta gtagccgaat tgcgcgatct tcgccatggc gcaggcgatg
     1801 acgaacacca cgtcggccac gatctgcacg agggccgcga tgaacgtcag ctggatgccc
     1861 gtcgcctcga cggcctcgtc cttcttcagg atggagatca gccagacggc cgcggcgccc
     1921 aggaggcacg cgttgcccag atagaacgcc acgagcgcca ggccccacgc gggacgggcc
     1981 ggcatgaggt aggagtgcgc ggtggcgacc atcatgagca cggcaaccgc gagggtcgcc
     2041 caggccagcg ccttgggaac gggcttgccg cctcgaagga ccacgaacca tgcgacgatg
     2101 acgatggcca gcgccacgca tccgatgagc tcctgggtga tgcccgacgt gatgtgcccg
     2161 aagccgttga agatgcgctc ccagtgctcg aggtgcagga aggctccgat gccgcctacc
     2221 accagcgaga cggcggacgt gatgagcgcg gtcatctgca ggttcctgcc ctggcccttg
     2281 agcgccagga tggacatgcc gcccagcgtg ccgcaggtca agcatacgaa cagcgtgaag
     2341 atgacgagag gccactgcgt ttccattact catcacctcc ttcccagtga tggtcgcgta
     2401 ggatgtagga gtacgacggc ttgttgccca cgtcgggcag cttgtgcacg tcggcgtcgg
     2461 tgaacggctc caggaactcc accatcttgc ggcgcttgcc cagcgggtcc tcggagccct
     2521 cgaacgactc gtagccggcc tcggtgtcgc cgatccagcg ggcgttgccg ccgcactggc
     2581 tgacgcactg gggcagctcg ccctgcgaca gcttctgctc gcacagcgtg cacttctcga
     2641 cgaccttctc ctcctcgttg aggtagcgga cgccgtaggg gcaggccatg acgcagaact
     2701 ggcagccgat gcacttctcc ttgtcgatct ggatggagcc gtcctcggcc acgtgcgacg
     2761 cctcggtggg gcacaccttc acgcactcgg ggttctcgca gtgctggcag ctgatgggca
     2821 ggaagtacat gtacacgtcg gggtactgcc ctgatccgcc cgggatgggg ttcggcccca
     2881 cgcgcaccac tttgttccag tagctgccga tgggcacgtt gttgaccgcc ttgcaggcga
     2941 ccgagcacgc caggcagccc acgcaccggt tgaggtcggt gatgatagcc ttcgtcatgg
     3001 ttcagtgatg gtgatggtga tggtgatgct cgagtgcggc ctccctccct tcgtaggtag
     3061 gcagccattc cttcagacgg gggtcgtcgg aggtatggat gatgggcgtg ccggtggagt
     3121 cgcacggcac ggggttgttg aacggcgagt tctcgggcgt ggccttgtag atcttcacct
     3181 ggtaggcgcg caggttggac gtgccggagt gcgggtcctg ggcgtagtgg tcgatcagct
     3241 ggttcacctg ggagaactgc cagccgtggc cggcgtcctt cacctcgggg taccaccacg
     3301 tgtgctcgag gttgatgacg tcttccttca cgccgtagta caggtcagcc acttcgcgga
     3361 tcttgcccca ctcggtttcg atccacaccc agtcgccctg ctcgatgccg tactcggcgg
     3421 ccgtcttcgg gttgatctcc acgcgcggca cgggccacag ctcgcggcac cagggaagct
     3481 ggcgatgctc ggagtggaag tacaccggga tgcgacggcc ggtggtggcg gtcagcgggt
     3541 actccttgat gcggtcgccg tccttcgggc cgtgaggcgg ctcggtgtag gtgggaagcc
     3601 tccactcggg atggtcggga tggtgcgatt ccatgacggt ggaccagatc tcctgcttca
     3661 tcgtcggggt gaaccagccc ggcttccagt cgccgatgcc cttgccggcc gtgtagtcga
     3721 ggcggcccca cacgcggtcg cgtgcgcgca tcgcgccggt ctggtagcgg cggtacgtgc
     3781 cccagttgcg cggctcgatg tccttcgcct gccaccagcc gtgctcctgg aaggccttga
     3841 cgtactcgtc ccacgtcttg tacttgggcg tgacctcggc catcggaacg ccgtggcgct
     3901 cgaacgtggg cttgccgttc tcgacgtgcc acgtggtgta ggtcagctcg tcgtcggtga
     3961 gcagcttgat ggagtcgtcc agctgccagt tgatgtcggg ccactcgttg cccggctcgt
     4021 cggtccactt ccagttcatg cggcgggcga ggtccatgac gatctcggga tcgtacttgg
     4081 cttccgcggg cggctgcacg cacttgaccg tggcgcccat ggcgccggcg gaaccttggc
     4141 tggcgcgcgg cgagttgagc tcgatccaat gggccaccgg caggatgatg tcggcggtgt
     4201 cgttctgcgg cgtgtgccac aggttgaggt ccacgtagaa gtcaagcgag cacagggcct
     4261 cccaggcgcg cgtggtgttc gtctggctca tgaagttgcc ggactcgttc cagagggcgc
     4321 gcaccgggta ggggtcgccc gtgatgacgg cgtcccacag cgaatggctg tcgcaccagt
     4381 actgccacca gcccagaagc gggaacttgt cgatgccgat ctgcttgcgg ttgacttcct
     4441 ccggcggggt ggtggcgccg ggggcccagg cgctgaagcc ctggaggtcg ccgtcgatgg
     4501 ggacgatggt cgggccgcgg ttgccgcccg gggtgtccat gttgccggtg atgcccacga
     4561 ggttgtcgaa ggcgcggcag ttctggatgg cgctgcagaa gtgctcgacc gccagcatgt
     4621 actggatgcc gccgttgccg tagccggtgg acgggtcgat gcgcgtgccg taggccgtgg
     4681 ccgccgcctc gatctcggag gcggggatgc cggtgatctc ggccgccacc tcgggcttgt
     4741 actcggcggc gcgggccttg tagtgctccc acaccggctt gaccttcacg gtcttgccgt
     4801 ccttcagcgt gatctcgaac tcgccttcca gcgcggggtc gatggcgggg tcgaacggca
     4861 tgggatcggg cagccagccc tgcgtctggc ccggcagcag gttgtcctgc gcggcctcgc
     4921 ggccttggcg cgccttctcg gagtcgtagt tctcgccctc ccagaagccg ccggtctcgt
     4981 cgatgacgcc ttcctggtcg gcgttgaacc aggtgaacgc gccgtactcg tgctcgacgc
     5041 cctcggcctt cagcttctcc cagttgttgt cgtacacgag gaacttatag gggctgccgc
     5101 cctccttgat gtcgctttcc ttgagcagcg cggttttcac gtcccagtag gaaccgtcgg
     5161 tgcgcacggt ggggaagccg gagggctcca tgtcctcgca cacgaggaac ggggcgttcg
     5221 tccacttctt cacgtacagc tcgtcgtaga gcttcttctc gatgatgacg ttcgtccagg
     5281 ccagggccag ggcgccgtcg gtgccggggc gcaggtgctg ccagtagtcg gcttccttgc
     5341 ccatgttggc catgcggggg tcgacgctga tgtgcacgtc ggcgcgcgat gccacgtcca
     5401 ccgtggtgcg gcaggagtcg tcgtagttgg acagctcgga cgcgccgccc cactgcacgt
     5461 acactttcgg gcgcgtgatg gtttccatcc acgacatggc gaactgcgac accatggtgg
     5521 tggcgaagtg gcgcgggccc ttgcagatct gccaggcctc aacgttgttc ggcgtttcca
     5581 gcatgttctt caggatggac tccgagtgca tgcaccagat gcgcgacgtg cccacctggc
     5641 aggcgatggc ctcgccgccg tgcttcgcct tgatgtccat gaagttgtcc acgatggact
     5701 gcatggcctc gtcccacgag atgcgctgcc agccgggctc ctcgcccttc gggttcgtgc
     5761 gcttcatggg atagtgcaga cggtcggggt ggtacgccgc ctggatggac gactgcgact
     5821 tcgtgcagca gttgcccatg gactggaacg ctccctcgtc gccctcgacg cgtatggccc
     5881 gcccgttctg gacgatgacc ttgacgccgc actccatctt gccgcagccg cggcagcacg
     5941 ttttcacggc cacggtgtcg ttgccggaca cgctgctgta cgtctcttcg gccagcgcgg
     6001 tgtgcgtcga agcgccgaac gccgcggctg ccgcaccggt gatggcggcg gtcttgacga
     6061 acgtgcgtcg tgacatggtc aggttaccca tactcctcct ccttctttct cctcgttctc
     6121 gtcttgctcg tcttcgttcg tgcgatcggg gaggggggct gccgggtgaa gcctcgggct
     6181 ccgaaccggt gcaaagtccc cgtgtcgctt gggccgacca tacggatgcg agggggtgca
     6241 gagcatcgtc caaaggttgg aaagcaggtt cgaccgcatc ataaattctt atgatcttgt
     6301 gatcatgaaa aattcgatag tgctttgaac tggggaaact ttaaccgtag gtcataaatt
     6361 attctgaaac tagaatagtt tcctcaaaga gggccgctca tcttttccgt ataatcgaac
     6421 ccgtcgacag aggggtcggc gcgagcgacg ttatgtacgg gctcacaggg ggtggggcat
     6481 ggaaaagctt cggagtgcat gggataccgc gttggggacg agcctttccc tcaagcgcat
     6541 cgcgggcgcg gccttgcaat ggctttggat gctcatgatg ttctacagca tcgtgccgta
     6601 cggcttctcg tcggacgtgc gcggctcgct gtacgtcagc ctgatcatat cgctggtggc
     6661 catggtggtg accatgctgt tcgtctccgt catcgtgcgg cgcgagcggg tgagcggcaa
     6721 ccgctccatc gtgctgggat cggccatcat gatgagcgcg ggcagcgtgc tcacgccgtt
     6781 ctccgatcct accacgcccg tcggcatgct ggtgctgggc ctgagcgcgg tcatgaccgg
     6841 cacgggctcg gccgtgctgt tcttgtgctg gatcgagctc gagtcggggc tgggaggccg
     6901 cttggcgctt gtggagcttg cctcgtcgct gtgcatcgcg ttcgtcgtgg ggttcttgct
     6961 gatcgtaggg ccggcgatac ccgtcatcgt gctcatcgtt gtcatgcccg tgctttcggc
     7021 ggcagctctc aggcgcagcg cgcccgatac gccgcagctg ccgcgcgagc ccgagcagcc
     7081 tttgtcgcgc catacggcga ccctgttcgc gaaggcgctt ctgggcgcgg tgctcatcgg
     7141 catgttgatg gggttcttcg acgtggtgtc ggggttcaaa acctacgagg tgcaggacat
     7201 cttcggcacg tacctgtttc tggcgggctt catcggagcg ttggccatct gcctgatagc
     7261 cgtgttcctg cagcgcgaca gcgtgttctt ctcgtaccgc gtctccatgc tgatgctgtg
     7321 cttgggatgc ttgtccacgc cgttcatggc cgacaacaac acgtattcga gcgcgcttat
     7381 cttcggcggc taccactgct tcgtgctggt gctgtgcgtg gtgtgcatcg acgtggcgtc
     7441 cagcttccgc gtgagcccgg cgcggacgat cggcctgggg ttcgtggcgc tgtacggcgg
     7501 cgagaccgca ggccagctgt tcgcccattc gctggaggcg accggggtca gcatgttcga
     7561 cctcacgctg gtcacgctgg tggccgtgtc gctgctgttc atcgcgcatt tgttcctgtt
     7621 cacggagacc gacctcatca aaatcggcat cggcgaggtc agcctgatgg gcacggtgcc
     7681 cgaagaggcc gccgacgacc cggccgaccc ggtggatccg tgcgcgctga tcgcggagcg
     7741 cttcgccctg tcgccgcgcg agaccgacgt gcttccgctg ctgctggagg gccgcaccat
     7801 ctcgcgcatc caggagacgc tgttcatctc ggcgggcacg gtgagcacgc atatccgcca
     7861 catctaccag aagacgggcg tggacaatcg ccaggagctc atcgacctgg cccacgagac
     7921 ggccgcgcag gacgaggcgg tctgaggcga tggagacggc gtatctgcac gagttcgcga
     7981 aggtggccga ttgcggcagc ttcacggccg ccgcgcgcga gctgcatctg acgcagtcga
     8041 cgctgagcaa gcacgtggcc ttgttggagc gcgaattcgg ggccgacctg ttcgtgcgcg
     8101 accgcagcgg cgtgagcctg accgaggcgg gcggcgtgct gtacgcccag gcgcggcaga
     8161 tggagaagct gctgcgcgcc accgagctgc tggtgcgcgc ggcgcgcgac gggcagggtt
     8221 cggcgggcgc gggggttgcg ggcgcgacgg cgggcgcagc ggcgggcggg ggtgccgtcg
     8281 acggctcgcc tgcggccgcg tgcgacacgg cgcttcgctg caagtgccgg ctcgcggccg
     8341 agcgctgcgg gctcgaccgg cgcgagctgg gcgcgctcat cctgtacgtg gaggagcgcg
     8401 gcttcgaggc gatccagggc gagctggcgc tgacgcgcga cgaggtggcc gacgtgctgg
     8461 gcagcgtgta ccgcaagctg ggcgtgggcg acaagcagga ggcgctcgat ttcatccatt
     8521 ccgtttcgga atgatggtag acgcgcccgc ggcggttggg catactgcgt agaagtgaaa
     8581 gcgctatgaa gctggggggc ttcgagcaaa gggcttcatc cgaagaatgg atggagaccg
     8641 aatctccaac ggtctgacga ccgcgcgaac cttcgctgac ttttcctgac cggtgatttt
     8701 gaaatgatga aaggactgat agatatgagc gatgttgatg tattcgtgat cgatgacgat
     8761 ttcgatcccc tggccgacct gagcctggac gacggcgagc gggacgatgc cgaggcggat
     8821 tacctgccgc ccatccccga tgccgacaag agcgtggtgc ctcccgtggt gccgttgtct
     8881 gcgaccgagc gcatcgagaa gctgctggcg ggcattcccg gccagcagtt ccgcctgctg
     8941 cacgccgtgg aattctgcac cgagccgaag acgatggacg aggccgtggc caatttggac
     9001 gcggcgtatc cgaacacgac gagcgtgtac ggctcggcgc aggtggtgca gctgctcgag
     9061 cgcgacggcg cgctggagcg catcgtggac gaggacgccg cccatgcggc cgccggcgac
     9121 gctgccgata cgcccgccga ggacgagggc ttcatctcgg tgacgcccgc tccgccgtgt
     9181 cactaccgtg ccacgcaagc gggcctcgat gccgtcgcgg cgcacgtcaa cgagggcctc
     9241 gtggcggagc ggatcagcga agacgagcgc tatctgccca tcttccagcg cgtgcttgag
     9301 atgtgcgcgc gcgaaggagg ctgccccacc aaggagctgg accaggcggt ggacggcgac
     9361 ccgctgtgcc aagagccgcg ccgcttctgc gggttcttcc gcggcaagct ggaagaaaca
     9421 ggcgccatcg aatggcgcga cgcctggacc attaccgatc tggggcgcag cgtgctcgcg
     9481 tccggcttgt tcgccgccgc ttccccgtcc gaacgctaga gaaggagcca cccatgtccc
     9541 acgatcagga aaccaccatc gccgacgtgc tgcgcggccg catggcaacc taccagttcc
     9601 tctcgcgcct gttccgcgtg gaggtggacc aggagctgta cgacacgctg atgtccatgc
     9661 gcttcccggc gaacaccggc aacgcgctgg tggacgaagg ctaccggatg atctgcggtt
     9721 atctgagcca agccgacggc acggtgctca ccgagctggc ggtggactac gtgcgcgcgt
     9781 tcatcgggtc gggcaacgac ggcttctcgg ccgcgtaccc gtacgagtcg gtgtacacga
     9841 gcccgaagcg cctcatgatg caggacgccc gcgacgaggt gctggtgctg taccgcgcct
     9901 tcggtctgga caagcaggaa agctggaagg agggcgagga ccatatcgcg ctggagctgg
     9961 agttcgagca gatcctatgc gagcgcgcta tcagggcgta cgaggccggc gacgaggacg
    10021 agtgcctgaa gctgctgctg tcgcagcgca acttcctgga ggaccacctg ctggcgtggt
    10081 accccatgat ggcggccgac ttgcagaagt tcccgcagac cgacttctac aagggcctgg
    10141 gcaagctgac cgacggcttc ctgcgcaacg accgcgaatt tttggacgcc gtgctgtctg
    10201 agaacgaagc ggattgccat ccagagcgga ggccgcaggc cgaagccgaa ggatcccgtg
    10261 cggcgtcggc cgaaaccgaa gtcgaggttg catgatggac gagcacggca tcgagcccaa
    10321 gcgcgatacg ggcccctgcg tgtcgcgccg cacgttcgcc ctgggagccg tgggagcgtg
    10381 cgccgtcatc gggctgggcg gcgtgaagta cctgcccagt gccacgttgc tgcgcccgcc
    10441 gggcgggcag gacgaggagc agctggcgcg cggctgcctg cattgcgaga agtgccgcga
    10501 ggtgtgcccg aagcatgcca tcgctcctgc tcatcttgag gacggcatct tgaacgcgcg
    10561 cacaccgcgc atggacttca aaagcggctg gtgcgatttc tgcgagaacg agccgggcgg
    10621 gccgaagtgc gtcgcggcgt gcccgacgca tgcgctttcg tgccccgacc cgtcgcaggc
    10681 gatcatcggc aaggcggaga tcaaccgcga ctggtgcctt gccgcgaagg gcatgggctg
    10741 ccacgagtgc gtcgacgtgt gcaactacga ggcgctcgag ctgggcgccg acaacgtgcc
    10801 ggtggtggac gtggatgcgt gcaacggctg cggcgcctgc gagctggcgt gcatctcgct
    10861 gtcggcaggt tccatcacgg ccggcgccac cgatcgcgcc atcgtcgtcc gtccgaccga
    10921 agaagtggag gcgtgatcag gatgaagaag ctcaaaacct atcggctgcg gttcctgatc
    10981 atgctggcgc tgctggccgt ggtggccgtg gggtacttca cggccggcgg catcggcaac
    11041 ttctgcggca tcggcttcga gtccatcacg ctgctgtgcc ccctaggcgc gctgctggcc
    11101 atgatcgccg agcgcacagc catcccgatg gccgtgatca gcgttgcggt cgtgctgctg
    11161 gtgtgtatcg tgctaggcaa ggtgttctgc gcctgggcgt gccccgtgca cttcctgtcg
    11221 cgaggaagga agaaggggga taagggggac aaggagggta aagggggcaa ggttgcttcc
    11281 ccttgctccg cgtgcgcttc cccttgcggc aaagccaagg gcatcaagat cgacagccgt
    11341 cacggcatcc tcgctgcggc gttgggctcc acgctcgtct tcggcttccc ggtgttctgc
    11401 ctcgtctgcc cgatcggcct cacgttcgcc acggtgctgc tggtcatgcg cctgttcgcg
    11461 ttcggcgacg ccacgtggac ggtcgtcgcc ttcccggcca tcatcgcgct ggagatcctg
    11521 ctgctgccga agtggtgcca gcgcttctgc ccgttgggcg cgttgctttc gctgttcagc
    11581 gggctgaacc ggacgttccg cccccaggtg gacgccgaga agtgcctgcg cgagggtcgc
    11641 ggcaaggcct gcaacctgtg cgagcaggca tgccccgagg gcatcaacct gcacgatgtc
    11701 gcggcgggcg agaccacgct caacgactgc agcaagtgcc gcgcgtgcgc cgacgtgtgc
    11761 cccgagcgcg ccatcacgtt cccgttgctg ccgaagaagc aggcgcaggc gacgccgctg
    11821 cccgtcgacg acgagatccc ggaaagggga taggccatgt ccctgatcaa cggcctgctg
    11881 gccctgttgg gcgagatcga gagcgatgcc atcgccgtgc atgaggaacg ctgcatctcg
    11941 gttcgcaacc gcaacgccga ctgcctgcgc tgcgtggagg catgcacgtc gggcgcgctg
    12001 gcgtaccgcg cgggcgagct gctcgtagag cccgagcgct gcatcggatg cggcacgtgc
    12061 gccacggcgt gccccacctg cgccatcgag ctgcgcaacc cgacggatgc cgagctgacg
    12121 gcgcagctca agcactccat cgtggcgacg aaaggccatc cggtcatcgt gtgcgagcag
    12181 gcgctcgagg cggccaacgt cgcggccgac gatgcgtcgg ccgcgtgcgc ggtgccgtgc
    12241 ctggggcgca tggacgagtc ggcgctcgtg gggttggcgg cgtaccgcgc cttcgacgcg
    12301 acgctggcct gcggttcgtg cgagacgtgc ccacatgctc cgggcggcgc gctcgtgcgc
    12361 gaggtggtgg agggcgcgcg caacctgctt gaggcgttcg gatcctccat gcccatcgac
    12421 ctggaggaat gcgtgcccga gcgcgtgttg gagcccggcg ggtcgcacgg ccaggcaggc
    12481 agcgacgggg tggggcggcg cgacttcttc cgctcggcca aggacgcatc gacccgcgca
    12541 gctggcgcgg cggtggccga ggagttgggg atggtcgagg ccgagcccgt tcctgcggcg
    12601 catcgcaaag tgggcgcaga tgggacgctg tcgcagttcg tccccacgcg ccgcgtgcgg
    12661 ctgtacaact acctgcagca cgtggggcaa ccgacggccg acgaggtgga gacgcgtgtc
    12721 atcggcgccg tgtccatcga tgcccagaag tgcagttcgt gccgcatgtg cgcggtgttc
    12781 tgtcccacgg gcgccatcaa gaagctcgac gagggcggcg tgttcggcat cgtgcaccgt
    12841 ccgagcgcct gcatgcagtg ccgcctgtgc gagcacatct gtcccgagca ggccatcgcg
    12901 gtgagcgggc gcgtgcccat caagcagttc atgggcaagg aagccgtgtg cttcgctatg
    12961 aagcgtccaa cctggacccc gaacaagccc tcatcgatgt acgataagct gcacgcgacg
    13021 ctcggcgaac acttggagat gtgcatgttc tagactagtc gactcacact ggctcacctt
    13081 cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat
    13141 ccggcttttt tattatttaa ttcctgttat aaaaaaagga tcaattttga actctctccc
    13201 aaagttgatc ccttaacgat ccctgcagga gaaggccatc ctgacggatg gcctttttga
    13261 agctttctag aatcgggggc ccccgggggg attctagaac aatggaagtc ctcgtctcgc
    13321 cctcgaatta gcccgcttaa tgagcgggct ttttttgaat taattctcgc gagctggcac
    13381 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt aagttagcgc
    13441 gaattgcaag ctggccgacg cgctgggcta cgtcttgctg gcgttcggga gcagaagagc
    13501 atacatctgg aagcaaagcc aggaaagcgg cctatggagc tgtgcggcag cgctcagtag
    13561 gcaatttttc aaaatattgt taagcctttt ctgagcatgg tatttttcat ggtattacca
    13621 attagcagga aaataagcca ttgaatataa aagataaaaa tgtcttgttt acaatagagt
    13681 ggggggggtc agcctgccgc cttgggccgg gtgatgtcgt acttgcccgc cgcgaactcg
    13741 gttaccgtcc agcccagcgc gaccagctcc ggcaacgcct cgcgcacccg ctggcggcgc
    13801 ttgcgcatgg tcgaaccact ggcctctgac ggccagacat agccgcacaa ggtatctatg
    13861 gaagccttgc cggttttgcc ggggtcgatc cagccacaca gccgctggtg cagcaggcgg
    13921 gcggtttcgc tgtccagcgc ccgcacctcg tccatgctga tgcgcacatg ctggccgcca
    13981 cccatgacgg cctgcgcgat caaggggttc agggccacgt acaggcgccc gtccgcctcg
    14041 tcgctggcgt actccgacag cagccgaaac ccctgccgct tgcggccatt ctgggcgatg
    14101 atggatacct tccaaaggcg ctcgatgcag tcctgtatgt gcttgagcgc cccaccacta
    14161 tcgacctctg ccccgatttc ctttgccagc gcccgatagc tacctttgac caccatggca
    14221 tcagcggtga cggcctccca cttgggttcc aggaacagcc ggagctgccg tccgccttcg
    14281 gtcttgggtt ccgggccaag cactaggcca ttaggcccag ccatggccac cagcccttgc
    14341 aggatgcgca gatcatcagc gcccagcggc tccgggccgc tgaactcgat ccgcttgccg
    14401 tcgccgtagt catacgtcac gtccagcttg ctgcgcttgc gctcgccccg cttgagggca
    14461 cggaacaggc cgggggccag acagtgcgcc gggtcgtgtc ggacgtggct gaggctgtgc
    14521 ttgttcttag gcttcaccac ggggcacccc cttgctcttg cgctgcctct ccagcacggc
    14581 gggcttgagc accccgccgt catgccgcct gaaccaccga tcagcgaacg gtgcgccata
    14641 gttggccttg ctcacaccga agcggacgaa gaaccggcgc tggtcgtcgt ccacacccca
    14701 ttcctcggcc tcggcgctgg tcatgctcga caggtaggac tgccagcgga tgttatcgac
    14761 cagtaccgag ctgccccggc tggcctgctg ctggtcgcct gcgcccatca tggccgcgcc
    14821 cttgctggca tggtgcagga acacgataga gcacccggta tcggcggcga tggcctccat
    14881 gcgaccgatg acctgggcca tggggccgct ggcgttttct tcctcgatgt ggaaccggcg
    14941 cagcgtgtcc agcaccatca ggcggcggcc ctcggcggcg cgcttgaggc cgtcgaacca
    15001 ctccggggcc atgatgttgg gcaggctgcc gatcagcggc tggatcagca ggccgtcagc
    15061 cacggcttgc cgttcctcgg cgctgaggtg cgccccaagg gcgtgcaggc ggtgatgaat
    15121 ggcggtgggc gggtcttcgg cgggcaggta gatcaccggg ccggtgggca gttcgcccac
    15181 ctccagcaga tccggcccgc ctgcaatctg tgcggccagt tgcagggcca gcatggattt
    15241 accggcacca ccgggcgaca ccagcgcccc gaccgtaccg gccaccatgt tgggcaaaac
    15301 gtagtccagc ggtggcggcg ctgctgcgaa cgcctccaga atattgatag gcttatgggt
    15361 agccattgat tgcctccttt gcaggcagtt ggtggttagg cgctggcggg gtcactaccc
    15421 ccgccctgcg ccgctctgag ttcttccagg cactcgcgca gcgcctcgta ttcgtcgtcg
    15481 gtcagccaga acttgcgctg acgcatccct ttggccttca tgcgctcggc atatcgcgct
    15541 tggcgtacag cgtcagggct ggccagcagg tcgccggtct gcttgtcctt ttggtctttc
    15601 atatcagtca ccgagaaact tgccggggcc gaaaggcttg tcttcgcgga acaaggacaa
    15661 ggtgcagccg tcaaggttaa ggctggccat atcagcgact gaaaagcggc cagcctcggc
    15721 cttgtttgac gtataaccaa agccaccggg caaccaatag cccttgtcac ttttgatcag
    15781 gtagaccgac cctgaagcgc ttttttcgta ttccataaaa cccccttctg tgcgtgagta
    15841 ctcatagtat aacaggcgtg agtaccaacg caagcactac atgctgaaat ctggcccgcc
    15901 cctgtccatg cctcgctggc ggggtgccgg tgcccgtgcc agctcggccc gcgcaagctg
    15961 gacgctgggc agacccatga ccttgctgac ggtgcgctcg atgtaatccg cttcgtggcc
    16021 gggcttgcgc tctgccagcg ctgggctggc ctcggccatg gccttgccga tttcctcggc
    16081 actgcggccc cggctggcca gcttctgcgc ggcgataaag tcgcacttgc tgaggtcatc
    16141 accgaagcgc ttgaccagcc cggccatctc gctgcggtac tcgtccagcg ccgtgcgccg
    16201 gtggcggcta agctgccgct cggacagttc gaggctggcc agcctgcggg ccttctcctg
    16261 ctgccgctgg gcctgctcga tctgctggcc agcctgctgc accagcgccg ggccagcggt
    16321 ggcggtcttg cccttggatt cacgcagcag cacccacggc tgataaccgg cgcgggtggt
    16381 gtgcttgtcc ttgcggttgg tgaagcccgc caagcggcca tagtggcggc tgtcggcgct
    16441 ggccgggtcg gcgtcgtact cgctggccag cgtccgggca atctgccccc gaagttcacc
    16501 gcctgcggcg tcggccacct tgacccatgc ctgatagttc ttcgggctgg tttccactac
    16561 cagggcaggc tcccggccct cggctttcat gtcatccagg tcaaactcgc tgaggtcgtc
    16621 caccagcacc agaccatgcc gctcctgctc ggcgggcctg atatacacgt cattgccctg
    16681 ggcattcatc cgcttgagcc atggcgtgtt ctggagcact tcggcggctg accattcccg
    16741 gttcatcatc tggccggtgg tggcgtccct gacgccgata tcgaagcgct cacagcccat
    16801 ggccttgagc tgtcggccta tggcctgcaa agtcctgtcg ttcttcatcg ggccaccaag
    16861 cgcagccaga tcgagccgtc ctcggttgtc agtggcgtca ggtcgagcaa gagcaacgat
    16921 gcgatcagca gcaccaccgt aggcatcatg gaagccagca tcacggttag ccatagcttc
    16981 cagtgccacc cccgcgacgc gctccgggcg ctctgcgcgg cgctgctcac ctcggcggct
    17041 acctcccgca actctttggc cagctccacc catgccgccc ctgtctggcg ctgggctttc
    17101 agccactccg ccgcctgcgc ctcgctggcc tgctgggtct ggctcatgac ctgccgggct
    17161 tcgtcggcca gtgtcgccat gctctgggcc agcggttcga tctgctccgc taactcgttg
    17221 atgcctctgg atttcttcac tctgtcgatt gcgttcatgg tctattgcct cccggtattc
    17281 ctgtaagtcg atgatctggg cgttggcggt gtcgatgttc agggccacgt ctgcccggtc
    17341 ggtgcggatg ccccggcctt ccatctccac cacgttcggc cccaggtgaa caccgggcag
    17401 gcgctcgatg ccctgcgcct caagtgttct gtggtcaatg cgggcgtcgt ggcctgcccg
    17461 ctctaatgcc cggttggcat ggtcggccca tgcctcgcgg gtctgctcaa gccatgcctt
    17521 gggcttgagc gcttcggtct tctgtgcccc gcccttctcc ggggtcttgc cgttgtaccg
    17581 cttgaaccac tgagcggcgg gccgctcgat gccgtcattg atccgctcgg agatcatcag
    17641 gtggcagtgc gggttctcgc cgccaccggc atggatggcc agcgtatacg gcaggcgctc
    17701 ggcaccggtc aggtgctggg cgaactcgga cgccagcgcc ttctgctggt cgagggtcag
    17761 ctcgaccggc agggcaaatt cgacctcctt gaacagccgc ccattggcgc gttcatacag
    17821 gtcggcagca tcccagtagt cggcgggccg ctcgacgaac tccggcatgt gcccggattc
    17881 ggcgtgcaag acttcatcca tgtcgcgggc atacttgcct tcgcgctgga tgaagtcggc
    17941 cttggccctg gccgattggc cgcccgacct gctgccggtt ttcgccgtaa ggtgataaat
    18001 cgccatgctg cctcgctgtt gcttttgctt ttcggctcca tgcaatggcc ctcggagagc
    18061 gcaccgcccg aagggtggcc gttaggccag tttctcgaag agaaaccggt aagtgcgccc
    18121 tcccctacaa agtagggtcg ggattgccgc cgctgtgcct ccatgatagc ctacgagaca
    18181 gcacattaac aatggggtgt caagatggtt aaggggagca acaaggcggc ggatcggctg
    18241 gccaagctcg aagaacaacg agcgcgaatc aatgccgaaa ttcagcgggt gcgggcaagg
    18301 gaacagcagc aagagcgcaa gaacgaaaca aggcgcaagg tgctggtggg ggccatgatt
    18361 ttggccaagg tgaacagcag cgagtggccg gaggatcggc tcatggcggc aatggatgcg
    18421 taccttgaac gcgaccacga ccgcgccttg ttcggtctgc cgccacgcca gaaggatgag
    18481 ccgggctgaa tgatcgaccg agacaggccc tgcggggctg cacacgcgcc cccacccttc
    18541 gggtaggggg aaaggccgct aaagcggcta aaagcgctcc agcgtatttc tgcggggttt
    18601 ggtgtggggt ttagcgggct ttgcccgcct ttccccctgc cgcgcagcgg tggggcggtg
    18661 tgtagcctag cgcagcgaat agaccagcta tccggcctct ggccgggcat attgggcaag
    18721 ggcagcagcg ccccacaagg gcgctgataa ccgcgcctag tggattattc ttagataatc
    18781 atggatggat ttttccaaca ccccgccagc ccccgcccct gctgggtttg caggtttggg
    18841 ggcgtgacag ttattgcagg ggttcgtgac agttattgca ggggggcgtg acagttattg
    18901 caggggttcg tgacagttag tacgggagtg acgggcactg gctggcaatg tctagcaacg
    18961 gcaggcattt cggctgaggg taaaagaact ttccgctaag cgatagactg tatgtaaaca
    19021 cagtattgca aggacgcgga acatgcctca tgtggcggcc ctcgcgagaa ttaattcaga
    19081 taaaaaaaat ccttagcttt cgctaaggat gatttctagc gatgaccctg ctgattggtt
    19141 cgctgaccgt agactggttg gcttggtttc atcagccatc cgcttgccct catctgttac
    19201 gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
    19261 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
    19321 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
    19381 atatcgtgcg acatcgcgcg cgcgcgcgcg gatgctagcg gcgcgccaaa aaaaagcccg
    19441 ctcatta
//
