LOCUS       sequence-494285-       14928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Dadh without accessory genes 
            (inactive) in Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14928)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 14928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80EN15-DadhA(cHis8)BC-DadRS-noAccs(ElA2)
FEATURES             Location/Qualifiers
     source          1..14928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(3005..3028)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      8552..8579
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      8711..8738
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(9170..10021)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(10008..10847)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(11358..12329)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            13568..13655
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      13997..14391
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(14554..14597)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            14693..14802
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttag
     1501 tacatcagga ccactgggta gcccagctgg tagatcagca cgcgaaacat cacggaggtg
     1561 gccagcgcgc cgatggcggc gacgaccatg agcgtcttgg aggcgcccac cttcttcttc
     1621 gccacgaagg cgcaggccgc agccacgatc acggagacga tggagcccca gaacatgggc
     1681 gctcccgcgc cggacaccat gacggtcatc aggctgtcga cgtgcgtggg ggccgtggtc
     1741 atcgatgtcg ggtcggcgta gtagccgaat tgcgcgatct tcgccatggc gcaggcgatg
     1801 acgaacacca cgtcggccac gatctgcacg agggccgcga tgaacgtcag ctggatgccc
     1861 gtcgcctcga cggcctcgtc cttcttcagg atggagatca gccagacggc cgcggcgccc
     1921 aggaggcacg cgttgcccag atagaacgcc acgagcgcca ggccccacgc gggacgggcc
     1981 ggcatgaggt aggagtgcgc ggtggcgacc atcatgagca cggcaaccgc gagggtcgcc
     2041 caggccagcg ccttgggaac gggcttgccg cctcgaagga ccacgaacca tgcgacgatg
     2101 acgatggcca gcgccacgca tccgatgagc tcctgggtga tgcccgacgt gatgtgcccg
     2161 aagccgttga agatgcgctc ccagtgctcg aggtgcagga aggctccgat gccgcctacc
     2221 accagcgaga cggcggacgt gatgagcgcg gtcatctgca ggttcctgcc ctggcccttg
     2281 agcgccagga tggacatgcc gcccagcgtg ccgcaggtca agcatacgaa cagcgtgaag
     2341 atgacgagag gccactgcgt ttccattact catcacctcc ttcccagtga tggtcgcgta
     2401 ggatgtagga gtacgacggc ttgttgccca cgtcgggcag cttgtgcacg tcggcgtcgg
     2461 tgaacggctc caggaactcc accatcttgc ggcgcttgcc cagcgggtcc tcggagccct
     2521 cgaacgactc gtagccggcc tcggtgtcgc cgatccagcg ggcgttgccg ccgcactggc
     2581 tgacgcactg gggcagctcg ccctgcgaca gcttctgctc gcacagcgtg cacttctcga
     2641 cgaccttctc ctcctcgttg aggtagcgga cgccgtaggg gcaggccatg acgcagaact
     2701 ggcagccgat gcacttctcc ttgtcgatct ggatggagcc gtcctcggcc acgtgcgacg
     2761 cctcggtggg gcacaccttc acgcactcgg ggttctcgca gtgctggcag ctgatgggca
     2821 ggaagtacat gtacacgtcg gggtactgcc ctgatccgcc cgggatgggg ttcggcccca
     2881 cgcgcaccac tttgttccag tagctgccga tgggcacgtt gttgaccgcc ttgcaggcga
     2941 ccgagcacgc caggcagccc acgcaccggt tgaggtcggt gatgatagcc ttcgtcatgg
     3001 ttcagtgatg gtgatggtga tggtgatgct cgagtgcggc ctccctccct tcgtaggtag
     3061 gcagccattc cttcagacgg gggtcgtcgg aggtatggat gatgggcgtg ccggtggagt
     3121 cgcacggcac ggggttgttg aacggcgagt tctcgggcgt ggccttgtag atcttcacct
     3181 ggtaggcgcg caggttggac gtgccggagt gcgggtcctg ggcgtagtgg tcgatcagct
     3241 ggttcacctg ggagaactgc cagccgtggc cggcgtcctt cacctcgggg taccaccacg
     3301 tgtgctcgag gttgatgacg tcttccttca cgccgtagta caggtcagcc acttcgcgga
     3361 tcttgcccca ctcggtttcg atccacaccc agtcgccctg ctcgatgccg tactcggcgg
     3421 ccgtcttcgg gttgatctcc acgcgcggca cgggccacag ctcgcggcac cagggaagct
     3481 ggcgatgctc ggagtggaag tacaccggga tgcgacggcc ggtggtggcg gtcagcgggt
     3541 actccttgat gcggtcgccg tccttcgggc cgtgaggcgg ctcggtgtag gtgggaagcc
     3601 tccactcggg atggtcggga tggtgcgatt ccatgacggt ggaccagatc tcctgcttca
     3661 tcgtcggggt gaaccagccc ggcttccagt cgccgatgcc cttgccggcc gtgtagtcga
     3721 ggcggcccca cacgcggtcg cgtgcgcgca tcgcgccggt ctggtagcgg cggtacgtgc
     3781 cccagttgcg cggctcgatg tccttcgcct gccaccagcc gtgctcctgg aaggccttga
     3841 cgtactcgtc ccacgtcttg tacttgggcg tgacctcggc catcggaacg ccgtggcgct
     3901 cgaacgtggg cttgccgttc tcgacgtgcc acgtggtgta ggtcagctcg tcgtcggtga
     3961 gcagcttgat ggagtcgtcc agctgccagt tgatgtcggg ccactcgttg cccggctcgt
     4021 cggtccactt ccagttcatg cggcgggcga ggtccatgac gatctcggga tcgtacttgg
     4081 cttccgcggg cggctgcacg cacttgaccg tggcgcccat ggcgccggcg gaaccttggc
     4141 tggcgcgcgg cgagttgagc tcgatccaat gggccaccgg caggatgatg tcggcggtgt
     4201 cgttctgcgg cgtgtgccac aggttgaggt ccacgtagaa gtcaagcgag cacagggcct
     4261 cccaggcgcg cgtggtgttc gtctggctca tgaagttgcc ggactcgttc cagagggcgc
     4321 gcaccgggta ggggtcgccc gtgatgacgg cgtcccacag cgaatggctg tcgcaccagt
     4381 actgccacca gcccagaagc gggaacttgt cgatgccgat ctgcttgcgg ttgacttcct
     4441 ccggcggggt ggtggcgccg ggggcccagg cgctgaagcc ctggaggtcg ccgtcgatgg
     4501 ggacgatggt cgggccgcgg ttgccgcccg gggtgtccat gttgccggtg atgcccacga
     4561 ggttgtcgaa ggcgcggcag ttctggatgg cgctgcagaa gtgctcgacc gccagcatgt
     4621 actggatgcc gccgttgccg tagccggtgg acgggtcgat gcgcgtgccg taggccgtgg
     4681 ccgccgcctc gatctcggag gcggggatgc cggtgatctc ggccgccacc tcgggcttgt
     4741 actcggcggc gcgggccttg tagtgctccc acaccggctt gaccttcacg gtcttgccgt
     4801 ccttcagcgt gatctcgaac tcgccttcca gcgcggggtc gatggcgggg tcgaacggca
     4861 tgggatcggg cagccagccc tgcgtctggc ccggcagcag gttgtcctgc gcggcctcgc
     4921 ggccttggcg cgccttctcg gagtcgtagt tctcgccctc ccagaagccg ccggtctcgt
     4981 cgatgacgcc ttcctggtcg gcgttgaacc aggtgaacgc gccgtactcg tgctcgacgc
     5041 cctcggcctt cagcttctcc cagttgttgt cgtacacgag gaacttatag gggctgccgc
     5101 cctccttgat gtcgctttcc ttgagcagcg cggttttcac gtcccagtag gaaccgtcgg
     5161 tgcgcacggt ggggaagccg gagggctcca tgtcctcgca cacgaggaac ggggcgttcg
     5221 tccacttctt cacgtacagc tcgtcgtaga gcttcttctc gatgatgacg ttcgtccagg
     5281 ccagggccag ggcgccgtcg gtgccggggc gcaggtgctg ccagtagtcg gcttccttgc
     5341 ccatgttggc catgcggggg tcgacgctga tgtgcacgtc ggcgcgcgat gccacgtcca
     5401 ccgtggtgcg gcaggagtcg tcgtagttgg acagctcgga cgcgccgccc cactgcacgt
     5461 acactttcgg gcgcgtgatg gtttccatcc acgacatggc gaactgcgac accatggtgg
     5521 tggcgaagtg gcgcgggccc ttgcagatct gccaggcctc aacgttgttc ggcgtttcca
     5581 gcatgttctt caggatggac tccgagtgca tgcaccagat gcgcgacgtg cccacctggc
     5641 aggcgatggc ctcgccgccg tgcttcgcct tgatgtccat gaagttgtcc acgatggact
     5701 gcatggcctc gtcccacgag atgcgctgcc agccgggctc ctcgcccttc gggttcgtgc
     5761 gcttcatggg atagtgcaga cggtcggggt ggtacgccgc ctggatggac gactgcgact
     5821 tcgtgcagca gttgcccatg gactggaacg ctccctcgtc gccctcgacg cgtatggccc
     5881 gcccgttctg gacgatgacc ttgacgccgc actccatctt gccgcagccg cggcagcacg
     5941 ttttcacggc cacggtgtcg ttgccggaca cgctgctgta cgtctcttcg gccagcgcgg
     6001 tgtgcgtcga agcgccgaac gccgcggctg ccgcaccggt gatggcggcg gtcttgacga
     6061 acgtgcgtcg tgacatggtc aggttaccca tactcctcct ccttctttct cctcgttctc
     6121 gtcttgctcg tcttcgttcg tgcgatcggg gaggggggct gccgggtgaa gcctcgggct
     6181 ccgaaccggt gcaaagtccc cgtgtcgctt gggccgacca tacggatgcg agggggtgca
     6241 gagcatcgtc caaaggttgg aaagcaggtt cgaccgcatc ataaattctt atgatcttgt
     6301 gatcatgaaa aattcgatag tgctttgaac tggggaaact ttaaccgtag gtcataaatt
     6361 attctgaaac tagaatagtt tcctcaaaga gggccgctca tcttttccgt ataatcgaac
     6421 ccgtcgacag aggggtcggc gcgagcgacg ttatgtacgg gctcacaggg ggtggggcat
     6481 ggaaaagctt cggagtgcat gggataccgc gttggggacg agcctttccc tcaagcgcat
     6541 cgcgggcgcg gccttgcaat ggctttggat gctcatgatg ttctacagca tcgtgccgta
     6601 cggcttctcg tcggacgtgc gcggctcgct gtacgtcagc ctgatcatat cgctggtggc
     6661 catggtggtg accatgctgt tcgtctccgt catcgtgcgg cgcgagcggg tgagcggcaa
     6721 ccgctccatc gtgctgggat cggccatcat gatgagcgcg ggcagcgtgc tcacgccgtt
     6781 ctccgatcct accacgcccg tcggcatgct ggtgctgggc ctgagcgcgg tcatgaccgg
     6841 cacgggctcg gccgtgctgt tcttgtgctg gatcgagctc gagtcggggc tgggaggccg
     6901 cttggcgctt gtggagcttg cctcgtcgct gtgcatcgcg ttcgtcgtgg ggttcttgct
     6961 gatcgtaggg ccggcgatac ccgtcatcgt gctcatcgtt gtcatgcccg tgctttcggc
     7021 ggcagctctc aggcgcagcg cgcccgatac gccgcagctg ccgcgcgagc ccgagcagcc
     7081 tttgtcgcgc catacggcga ccctgttcgc gaaggcgctt ctgggcgcgg tgctcatcgg
     7141 catgttgatg gggttcttcg acgtggtgtc ggggttcaaa acctacgagg tgcaggacat
     7201 cttcggcacg tacctgtttc tggcgggctt catcggagcg ttggccatct gcctgatagc
     7261 cgtgttcctg cagcgcgaca gcgtgttctt ctcgtaccgc gtctccatgc tgatgctgtg
     7321 cttgggatgc ttgtccacgc cgttcatggc cgacaacaac acgtattcga gcgcgcttat
     7381 cttcggcggc taccactgct tcgtgctggt gctgtgcgtg gtgtgcatcg acgtggcgtc
     7441 cagcttccgc gtgagcccgg cgcggacgat cggcctgggg ttcgtggcgc tgtacggcgg
     7501 cgagaccgca ggccagctgt tcgcccattc gctggaggcg accggggtca gcatgttcga
     7561 cctcacgctg gtcacgctgg tggccgtgtc gctgctgttc atcgcgcatt tgttcctgtt
     7621 cacggagacc gacctcatca aaatcggcat cggcgaggtc agcctgatgg gcacggtgcc
     7681 cgaagaggcc gccgacgacc cggccgaccc ggtggatccg tgcgcgctga tcgcggagcg
     7741 cttcgccctg tcgccgcgcg agaccgacgt gcttccgctg ctgctggagg gccgcaccat
     7801 ctcgcgcatc caggagacgc tgttcatctc ggcgggcacg gtgagcacgc atatccgcca
     7861 catctaccag aagacgggcg tggacaatcg ccaggagctc atcgacctgg cccacgagac
     7921 ggccgcgcag gacgaggcgg tctgaggcga tggagacggc gtatctgcac gagttcgcga
     7981 aggtggccga ttgcggcagc ttcacggccg ccgcgcgcga gctgcatctg acgcagtcga
     8041 cgctgagcaa gcacgtggcc ttgttggagc gcgaattcgg ggccgacctg ttcgtgcgcg
     8101 accgcagcgg cgtgagcctg accgaggcgg gcggcgtgct gtacgcccag gcgcggcaga
     8161 tggagaagct gctgcgcgcc accgagctgc tggtgcgcgc ggcgcgcgac gggcagggtt
     8221 cggcgggcgc gggggttgcg ggcgcgacgg cgggcgcagc ggcgggcggg ggtgccgtcg
     8281 acggctcgcc tgcggccgcg tgcgacacgg cgcttcgctg caagtgccgg ctcgcggccg
     8341 agcgctgcgg gctcgaccgg cgcgagctgg gcgcgctcat cctgtacgtg gaggagcgcg
     8401 gcttcgaggc gatccagggc gagctggcgc tgacgcgcga cgaggtggcc gacgtgctgg
     8461 gcagcgtgta ccgcaagctg ggcgtgggcg acaagcagga ggcgctcgat ttcatccatt
     8521 ccgtttcgga atgaactagt cgactcacac tggctcacct tcgggtgggc ctttctgcgt
     8581 ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt ttattattta
     8641 attcctgtta taaaaaaagg atcaattttg aactctctcc caaagttgat cccttaacga
     8701 tccctgcagg agaaggccat cctgacggat ggcctttttg aagctttcta gaatcggggg
     8761 cccccggggg gattctagaa caatggaagt cctcgtctcg ccctcgaatt agcccgctta
     8821 atgagcgggc tttttttgaa ttaattctcg cgagctggca cgacaggttt cccgactgga
     8881 aagcgggcag tgagcgcaac gcaattaatg taagttagcg cgaattgcaa gctggccgac
     8941 gcgctgggct acgtcttgct ggcgttcggg agcagaagag catacatctg gaagcaaagc
     9001 caggaaagcg gcctatggag ctgtgcggca gcgctcagta ggcaattttt caaaatattg
     9061 ttaagccttt tctgagcatg gtatttttca tggtattacc aattagcagg aaaataagcc
     9121 attgaatata aaagataaaa atgtcttgtt tacaatagag tggggggggt cagcctgccg
     9181 ccttgggccg ggtgatgtcg tacttgcccg ccgcgaactc ggttaccgtc cagcccagcg
     9241 cgaccagctc cggcaacgcc tcgcgcaccc gctggcggcg cttgcgcatg gtcgaaccac
     9301 tggcctctga cggccagaca tagccgcaca aggtatctat ggaagccttg ccggttttgc
     9361 cggggtcgat ccagccacac agccgctggt gcagcaggcg ggcggtttcg ctgtccagcg
     9421 cccgcacctc gtccatgctg atgcgcacat gctggccgcc acccatgacg gcctgcgcga
     9481 tcaaggggtt cagggccacg tacaggcgcc cgtccgcctc gtcgctggcg tactccgaca
     9541 gcagccgaaa cccctgccgc ttgcggccat tctgggcgat gatggatacc ttccaaaggc
     9601 gctcgatgca gtcctgtatg tgcttgagcg ccccaccact atcgacctct gccccgattt
     9661 cctttgccag cgcccgatag ctacctttga ccaccatggc atcagcggtg acggcctccc
     9721 acttgggttc caggaacagc cggagctgcc gtccgccttc ggtcttgggt tccgggccaa
     9781 gcactaggcc attaggccca gccatggcca ccagcccttg caggatgcgc agatcatcag
     9841 cgcccagcgg ctccgggccg ctgaactcga tccgcttgcc gtcgccgtag tcatacgtca
     9901 cgtccagctt gctgcgcttg cgctcgcccc gcttgagggc acggaacagg ccgggggcca
     9961 gacagtgcgc cgggtcgtgt cggacgtggc tgaggctgtg cttgttctta ggcttcacca
    10021 cggggcaccc ccttgctctt gcgctgcctc tccagcacgg cgggcttgag caccccgccg
    10081 tcatgccgcc tgaaccaccg atcagcgaac ggtgcgccat agttggcctt gctcacaccg
    10141 aagcggacga agaaccggcg ctggtcgtcg tccacacccc attcctcggc ctcggcgctg
    10201 gtcatgctcg acaggtagga ctgccagcgg atgttatcga ccagtaccga gctgccccgg
    10261 ctggcctgct gctggtcgcc tgcgcccatc atggccgcgc ccttgctggc atggtgcagg
    10321 aacacgatag agcacccggt atcggcggcg atggcctcca tgcgaccgat gacctgggcc
    10381 atggggccgc tggcgttttc ttcctcgatg tggaaccggc gcagcgtgtc cagcaccatc
    10441 aggcggcggc cctcggcggc gcgcttgagg ccgtcgaacc actccggggc catgatgttg
    10501 ggcaggctgc cgatcagcgg ctggatcagc aggccgtcag ccacggcttg ccgttcctcg
    10561 gcgctgaggt gcgccccaag ggcgtgcagg cggtgatgaa tggcggtggg cgggtcttcg
    10621 gcgggcaggt agatcaccgg gccggtgggc agttcgccca cctccagcag atccggcccg
    10681 cctgcaatct gtgcggccag ttgcagggcc agcatggatt taccggcacc accgggcgac
    10741 accagcgccc cgaccgtacc ggccaccatg ttgggcaaaa cgtagtccag cggtggcggc
    10801 gctgctgcga acgcctccag aatattgata ggcttatggg tagccattga ttgcctcctt
    10861 tgcaggcagt tggtggttag gcgctggcgg ggtcactacc cccgccctgc gccgctctga
    10921 gttcttccag gcactcgcgc agcgcctcgt attcgtcgtc ggtcagccag aacttgcgct
    10981 gacgcatccc tttggccttc atgcgctcgg catatcgcgc ttggcgtaca gcgtcagggc
    11041 tggccagcag gtcgccggtc tgcttgtcct tttggtcttt catatcagtc accgagaaac
    11101 ttgccggggc cgaaaggctt gtcttcgcgg aacaaggaca aggtgcagcc gtcaaggtta
    11161 aggctggcca tatcagcgac tgaaaagcgg ccagcctcgg ccttgtttga cgtataacca
    11221 aagccaccgg gcaaccaata gcccttgtca cttttgatca ggtagaccga ccctgaagcg
    11281 cttttttcgt attccataaa acccccttct gtgcgtgagt actcatagta taacaggcgt
    11341 gagtaccaac gcaagcacta catgctgaaa tctggcccgc ccctgtccat gcctcgctgg
    11401 cggggtgccg gtgcccgtgc cagctcggcc cgcgcaagct ggacgctggg cagacccatg
    11461 accttgctga cggtgcgctc gatgtaatcc gcttcgtggc cgggcttgcg ctctgccagc
    11521 gctgggctgg cctcggccat ggccttgccg atttcctcgg cactgcggcc ccggctggcc
    11581 agcttctgcg cggcgataaa gtcgcacttg ctgaggtcat caccgaagcg cttgaccagc
    11641 ccggccatct cgctgcggta ctcgtccagc gccgtgcgcc ggtggcggct aagctgccgc
    11701 tcggacagtt cgaggctggc cagcctgcgg gccttctcct gctgccgctg ggcctgctcg
    11761 atctgctggc cagcctgctg caccagcgcc gggccagcgg tggcggtctt gcccttggat
    11821 tcacgcagca gcacccacgg ctgataaccg gcgcgggtgg tgtgcttgtc cttgcggttg
    11881 gtgaagcccg ccaagcggcc atagtggcgg ctgtcggcgc tggccgggtc ggcgtcgtac
    11941 tcgctggcca gcgtccgggc aatctgcccc cgaagttcac cgcctgcggc gtcggccacc
    12001 ttgacccatg cctgatagtt cttcgggctg gtttccacta ccagggcagg ctcccggccc
    12061 tcggctttca tgtcatccag gtcaaactcg ctgaggtcgt ccaccagcac cagaccatgc
    12121 cgctcctgct cggcgggcct gatatacacg tcattgccct gggcattcat ccgcttgagc
    12181 catggcgtgt tctggagcac ttcggcggct gaccattccc ggttcatcat ctggccggtg
    12241 gtggcgtccc tgacgccgat atcgaagcgc tcacagccca tggccttgag ctgtcggcct
    12301 atggcctgca aagtcctgtc gttcttcatc gggccaccaa gcgcagccag atcgagccgt
    12361 cctcggttgt cagtggcgtc aggtcgagca agagcaacga tgcgatcagc agcaccaccg
    12421 taggcatcat ggaagccagc atcacggtta gccatagctt ccagtgccac ccccgcgacg
    12481 cgctccgggc gctctgcgcg gcgctgctca cctcggcggc tacctcccgc aactctttgg
    12541 ccagctccac ccatgccgcc cctgtctggc gctgggcttt cagccactcc gccgcctgcg
    12601 cctcgctggc ctgctgggtc tggctcatga cctgccgggc ttcgtcggcc agtgtcgcca
    12661 tgctctgggc cagcggttcg atctgctccg ctaactcgtt gatgcctctg gatttcttca
    12721 ctctgtcgat tgcgttcatg gtctattgcc tcccggtatt cctgtaagtc gatgatctgg
    12781 gcgttggcgg tgtcgatgtt cagggccacg tctgcccggt cggtgcggat gccccggcct
    12841 tccatctcca ccacgttcgg ccccaggtga acaccgggca ggcgctcgat gccctgcgcc
    12901 tcaagtgttc tgtggtcaat gcgggcgtcg tggcctgccc gctctaatgc ccggttggca
    12961 tggtcggccc atgcctcgcg ggtctgctca agccatgcct tgggcttgag cgcttcggtc
    13021 ttctgtgccc cgcccttctc cggggtcttg ccgttgtacc gcttgaacca ctgagcggcg
    13081 ggccgctcga tgccgtcatt gatccgctcg gagatcatca ggtggcagtg cgggttctcg
    13141 ccgccaccgg catggatggc cagcgtatac ggcaggcgct cggcaccggt caggtgctgg
    13201 gcgaactcgg acgccagcgc cttctgctgg tcgagggtca gctcgaccgg cagggcaaat
    13261 tcgacctcct tgaacagccg cccattggcg cgttcataca ggtcggcagc atcccagtag
    13321 tcggcgggcc gctcgacgaa ctccggcatg tgcccggatt cggcgtgcaa gacttcatcc
    13381 atgtcgcggg catacttgcc ttcgcgctgg atgaagtcgg ccttggccct ggccgattgg
    13441 ccgcccgacc tgctgccggt tttcgccgta aggtgataaa tcgccatgct gcctcgctgt
    13501 tgcttttgct tttcggctcc atgcaatggc cctcggagag cgcaccgccc gaagggtggc
    13561 cgttaggcca gtttctcgaa gagaaaccgg taagtgcgcc ctcccctaca aagtagggtc
    13621 gggattgccg ccgctgtgcc tccatgatag cctacgagac agcacattaa caatggggtg
    13681 tcaagatggt taaggggagc aacaaggcgg cggatcggct ggccaagctc gaagaacaac
    13741 gagcgcgaat caatgccgaa attcagcggg tgcgggcaag ggaacagcag caagagcgca
    13801 agaacgaaac aaggcgcaag gtgctggtgg gggccatgat tttggccaag gtgaacagca
    13861 gcgagtggcc ggaggatcgg ctcatggcgg caatggatgc gtaccttgaa cgcgaccacg
    13921 accgcgcctt gttcggtctg ccgccacgcc agaaggatga gccgggctga atgatcgacc
    13981 gagacaggcc ctgcggggct gcacacgcgc ccccaccctt cgggtagggg gaaaggccgc
    14041 taaagcggct aaaagcgctc cagcgtattt ctgcggggtt tggtgtgggg tttagcgggc
    14101 tttgcccgcc tttccccctg ccgcgcagcg gtggggcggt gtgtagccta gcgcagcgaa
    14161 tagaccagct atccggcctc tggccgggca tattgggcaa gggcagcagc gccccacaag
    14221 ggcgctgata accgcgccta gtggattatt cttagataat catggatgga tttttccaac
    14281 accccgccag cccccgcccc tgctgggttt gcaggtttgg gggcgtgaca gttattgcag
    14341 gggttcgtga cagttattgc aggggggcgt gacagttatt gcaggggttc gtgacagtta
    14401 gtacgggagt gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg
    14461 gtaaaagaac tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg
    14521 aacatgcctc atgtggcggc cctcgcgaga attaattcag ataaaaaaaa tccttagctt
    14581 tcgctaagga tgatttctag cgatgaccct gctgattggt tcgctgaccg tagactggtt
    14641 ggcttggttt catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag
    14701 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag
    14761 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata
    14821 caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc gacatcgcgc
    14881 gcgcgcgcgc ggatgctagc ggcgcgccaa aaaaaagccc gctcatta
//
