LOCUS       sequence-49430-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  TITLE     Gutkind Lab pCEFL plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCEFL
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(130..148)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(131..148)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(168..185)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    174..398
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      444..802
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(531..550)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     741..762
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggtgtcgtga ggaattggcg aagcttggta ccgagctcgg atccactagt aacggccgcc
       61 agtgtgctgg aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
      121 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
      181 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      241 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      301 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
      361 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
      421 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      481 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      541 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      601 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
      661 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
      721 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
      781 ctcggtctat tcttttgatt tataagggat ttt
//
