LOCUS       sequence-49431-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Acker MG, Kolitz SE, Mitchell SF, Nanda JS, Lorsch JR
  TITLE     Reconstitution of yeast translation initiation.
  JOURNAL   Methods Enzymol. 2007;430:111-45.
  PUBMED    17913637
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2007"; volume: "430"; pages: "111-45"
COMMENT     Sequence Label: pTYB2-eIF1
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgtcc attgagaatc tgaaatcatt
       61 tgatcctttc gccgacacag gagacgacga aaccgccact tcaaactata ttcatattcg
      121 tatccaacag agaaatggta gaaaaacttt aactacggtg caaggtgtcc cagaggaata
      181 tgatttaaag agaattctta aggtcctaaa gaaggacttt gcatgtaatg gtaacattgt
      241 caaggatcca gaaatggggg agattattca gttgcagggt gaccaaagag caaaggtttg
      301 cgaatttatg atctcccaac tgggattgca aaagaagaac attaaaattc atgggtttcc
      361 cgggtgcttt gccaagggta ccaatgtttt aatggcggat gggtctattg aatgtattga
      421 aaacattgag gttggtaata aggtcatggg taaagatggc agacctcgtg aggtaattaa
      481 attgcccaga ggaagagaaa ctatgtacag cgtcgtgcag aaaagtcagc acagagccca
      541 caaaagtgac tcaagtcgtg aagtgccaga attactcaag tttacgtgta atgcgaccca
      601 tgagttggtt gttagaacac ctcgtagtgt ccgccgtttg tctcgtacca ttaagggtgt
      661 cgaatatttt gaagttatta cttttgagat gggccaaaag aaagcccccg acggtagaat
      721 tgttgagctt gtcaaggaag tttcaaagag ctacccaata tctgaggggc ctgagagagc
      781 caacgaatta gtagaatcct atagaaaggc ttcaaataaa gcttattttg agtggactat
      841 tgaggccaga gatctttctc tgttgg
//
