LOCUS       sequence-494314-        9468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFR-mEGFP in mammalian cells regulated by a synthetic 
            microRNA-based dosage compensation circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9468)
  TITLE     miRNA circuit modules for precise control of gene expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-synmiR-L-mRuby-Pro-EGFR-mEGFP-4×19
FEATURES             Location/Qualifiers
     source          1..9468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    131..252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(168..187)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     222..241
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   278..418
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   278..407
                     /label=AAV2 ITR
     rep_origin      493..948
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(580..599)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     790..811
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(965..984)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1084..1106
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1144..1162)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1230..1334
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1335..1403,1404..2195)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSLIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2366..2954
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2855..2874
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   3016..3145
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     polyA_signal    3160..3208
                     /note="synthetic polyadenylation signal"
     ncRNA           complement(3412..3426)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(3588..4298)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     regulatory      4295..4304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4320..4531)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        4576..4879
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             join(5123..5194,5195..7057,7058..7126,7127..8752)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             join(8759..8761,8762..8764,8765..9468,1..10)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8804..8825)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9065..9084)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9412..9433
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtacaagtag agcttgaatt ataatgctta tctaaataga attataatgc ttatctaaat
       61 agaattataa tgcttatcta aatagaatta taatgcttat ctaaagggcc catcgatacc
      121 gagcgctagc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      181 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      241 caatgtatct tatcatgtct gcggaccgag cggccgcagg aacccctagt gatggagttg
      301 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
      361 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
      421 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
      481 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      541 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
      601 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      661 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
      721 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
      781 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
      841 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
      901 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
      961 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     1021 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     1081 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     1141 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     1201 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     1261 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1321 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1381 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1441 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1501 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1561 gcgcggtatt atcccttatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1621 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1681 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1741 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1801 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1861 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1921 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1981 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2041 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2101 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2161 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     2221 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2281 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2341 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2401 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2461 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2521 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2581 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2641 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2701 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2761 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2821 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2881 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2941 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3001 cttttgctca catgtcctgc aggcagctgc gcgctcgctc gctcactgag gccgcccggg
     3061 cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg
     3121 gccaactcca tcactagggg ttcctgcggc cgcacgcgtc acacaaaaaa ccaacacaca
     3181 gatctaatga aaataaagat cttttattat ttcttccaca agcctgcagg aaaaagaaag
     3241 aggatgaatg gtcaggaaaa gtgatttaat ttataccatt ttaattcagc tttgtaaaaa
     3301 tgtatcaaag agatagcaag gtattcagtt ttagtaaaca agataattgc tcgaattcta
     3361 gccccttgaa gtccgaggca gtaggcatag gaattataat gcttatctat acatctgtgg
     3421 cttcactata gataagcatt ataattcctg cgctcactgt caacagcaat ataccttctc
     3481 gagccttctg ttgggttaag aagtcgaagt aatcccagca agtgtttcca agatgtgcag
     3541 gcaacgattc tgtaaagtac tgaagcctca ttcaaactta cctgctactt atatagctcg
     3601 tccatgccac caccaaggtt gctgtatttg gcaactgcca cttctctttg cactacgtag
     3661 gtttcattgt cactctcctc gatcctttcc aggcggtgat caacggcatg gacaccgggc
     3721 atcttgatgt tcccgacggt cttttttgac ctgtaagttg tcacgaagtt gcagtgcaga
     3781 tggccaccac catcaacttt cagtgcgatg tcagtgtatc ctctcagacc accatctgct
     3841 ggatacatca tctctgtatt aggctcccaa cccttggtct tcttctgcat cacgggacca
     3901 ttggagggaa agtttacccc tctgaccttg acgttgtaga cgagctcgcc atcctcaagg
     3961 ctggtgtcct gcgtgacggt gacgactcca ccatcttcgt atctcgtaac tctttcccaa
     4021 gtaaaaccct caggaaagga ctgtttaaag aaatcaggga tgtcggccgg gtacttgata
     4081 aaggtacggc tgccatacat gaacgacgtg gcaagaatgt caaaggcaaa tggcaggggt
     4141 cctccctcga tgactttgat cctcatggtt tgcactccct cgtacggtct gccttctcct
     4201 tcacctgtgc atttgaattg gtggccgttg accgaacctt ccatgaccac cttcatacgc
     4261 atattttcct tgatcagctc ttcgccctta gacaccatgg tggcgtcgac tcacgacacc
     4321 tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata
     4381 tacggttctc ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag
     4441 tttaccccgc gccaccttct ctaggcaccg gttcaattgc cgacccctcc ccccaacttc
     4501 tcggggactg tgggcgatgt gcgctctgcc cactgacggg caccggagcc ctatgacttg
     4561 catagctgcg tatggcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4621 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4681 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     4741 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4801 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     4861 agtcatcgct attaccatga attgagggcg tcaccgctaa ggctccgccc cagcctgggc
     4921 tccacaacca atgaagggta atctcgacaa agagcaaggg gtggggcgcg ggcgcgcagg
     4981 tgcagcagca cacaggctgg tcgggagggc ggggcgcgac gtctgccgtg cggggtcccg
     5041 gcatcggttg cgcgcgcgct ccctcctctc ggagagaggg ctgtggtaaa acccgtccgg
     5101 aaaccactta agcttcgcca ccatgagacc tagcgggacg gcaggagctg ccctgttggc
     5161 cctattggcg gctctttgcc cagccagtcg ggctctggag gaaaagaaag tttgccaagg
     5221 cacgagtaac aagctcacgc agttgggcac ttttgaagat cattttctca gcctccagag
     5281 gatgttcaat aactgtgagg tggtccttgg gaatttggaa attacctatg tgcagaggaa
     5341 ttatgatctt tccttcttaa agaccatcca ggaggtggct ggttatgtcc tcattgccct
     5401 caacacagtg gagcgaattc ctttggaaaa cctgcagatc atcagaggaa atatgtacta
     5461 cgaaaattcc tatgccttag cagtcttatc taactatgat gcaaataaaa ccggactgaa
     5521 ggagctgccc atgagaaatt tacaggaaat cctgcatggc gccgtgcggt tcagcaacaa
     5581 ccctgccctg tgcaacgtgg agagcatcca gtggcgggac atagtcagca gtgactttct
     5641 cagcaacatg tcgatggact tccagaacca cctgggcagc tgccaaaagt gtgatccaag
     5701 ctgtcccaat gggagctgct ggggtgcagg agaggagaac tgccagaaac tgaccaaaat
     5761 catctgtgcc cagcagtgct ccgggcgctg ccgtggcaag tcccccagtg actgctgcca
     5821 caaccagtgt gctgcaggct gcacaggccc ccgggagagc gactgcctgg tctgccgcaa
     5881 attccgagac gaagccacgt gcaaggacac ctgcccccca ctcatgctct acaaccccac
     5941 cacgtaccag atggatgtga accccgaggg caaatacagc tttggtgcca cctgcgtgaa
     6001 gaagtgtccc cgtaattatg tggtgacaga tcacggctcg tgcgtccgag cctgtggggc
     6061 cgacagctat gagatggagg aagacggcgt ccgcaagtgt aagaagtgcg aagggccttg
     6121 ccgcaaagtg tgtaacggaa taggtattgg tgaatttaaa gactcactct ccataaatgc
     6181 tacgaatatt aaacacttca aaaactgcac ctccatcagt ggcgatctcc acatcctgcc
     6241 ggtggcattt aggggtgact ccttcacaca tactcctcct ctggatccac aggaactgga
     6301 tattctgaaa accgtaaagg aaatcacagg gtttttgctg attcaggctt ggcctgaaaa
     6361 caggacggac ctccatgcct ttgagaacct agaaatcata cgcggcagga ccaagcaaca
     6421 tggtcagttt tctcttgcag tcgtcagcct gaacataaca tccttgggat tacgctccct
     6481 caaggagata agtgatggag atgtgataat ttcaggaaac aaaaatttgt gctatgcaaa
     6541 tacaataaac tggaaaaaac tgtttgggac ctccggtcag aaaaccaaaa ttataagcaa
     6601 cagaggtgaa aacagctgca aggccacagg ccaggtctgc catgccttgt gctcccccga
     6661 gggctgctgg ggcccggagc ccagggactg cgtctcttgc cggaatgtca gccgaggcag
     6721 ggaatgcgtg gacaagtgca accttctgga gggtgagcca agggagtttg tggagaactc
     6781 tgagtgcata cagtgccacc cagagtgcct gcctcaggcc atgaacatca cctgcacagg
     6841 acggggacca gacaactgta tccagtgtgc ccactacatt gacggccccc actgcgtcaa
     6901 gacctgcccg gcaggagtca tgggagaaaa caacaccctg gtctggaagt acgcagacgc
     6961 cggccatgtg tgccacctgt gccatccaaa ctgcacctac ggatgcactg ggccaggtct
     7021 tgaaggctgt ccaacgaatg ggcctaagat cccgtccatc gccactggga tggtgggggc
     7081 cctcctcttg ctgctggtgg tggccctggg gatcggcctc ttcatgcgaa ggcgccacat
     7141 cgttcggaag cgcacgctgc ggaggctgct gcaggagagg gagcttgtgg agcctcttac
     7201 acccagtgga gaagctccca accaagctct cttgaggatc ttgaaggaaa ctgaattcaa
     7261 aaagatcaaa gtgctgggct ccggtgcgtt cggcacggtg tataagggac tctggatccc
     7321 agaaggtgag aaagttaaaa ttcccgtcgc tatcaaggaa ttaagagaag caacatctcc
     7381 gaaagccaac aaggaaatcc tcgatgaagc ctacgtgatg gccagcgtgg acaaccccca
     7441 cgtgtgccgc ctgctgggca tctgcctcac ctccaccgtg cagctcatca cgcagctcat
     7501 gcccttcggc tgcctcctgg actatgtccg ggaacacaaa gacaatattg gctcccagta
     7561 cctgctcaac tggtgtgtgc agatcgcaaa gggcatgaac tacttggagg accgtcgctt
     7621 ggtgcaccgc gacctggcag ccaggaacgt actggtgaaa acaccgcagc atgtcaagat
     7681 cacagatttt gggctggcca aactgctggg tgcggaagag aaagaatacc atgcagaagg
     7741 aggcaaagtg cctatcaagt ggatggcatt ggaatcaatt ttacacagaa tctataccca
     7801 ccagagtgat gtctggagct acggggtgac tgtttgggag ttgatgacct ttggatccaa
     7861 gccatatgac ggaatccctg ccagcgagat ctcctccatc ctggagaaag gagaacgcct
     7921 ccctcagcca cccatatgta ccatcgatgt ctacatgatc atggtcaagt gctggatgat
     7981 agacgcagat agtcgcccaa agttccgtga gttgatcatc gaattctcca aaatggcccg
     8041 agacccccag cgctaccttg tcattcaggg ggatgaaaga atgcatttgc caagtcctac
     8101 agactccaac ttctaccgtg ccctgatgga tgaagaagac atggacgacg tggtggatgc
     8161 cgacgagtac ctcatcccac agcagggctt cttcagcagc ccctccacgt cacggactcc
     8221 cctcctgagc tctctgagtg caaccagcaa caattccacc gtggcttgca ttgatagaaa
     8281 tgggctgcaa agctgtccca tcaaggaaga cagcttcttg cagcgataca gctcagaccc
     8341 cacaggcgcc ttgactgagg acagcataga cgacaccttc ctcccagtgc ctgaatacat
     8401 aaaccagtcc gttcccaaaa ggcccgctgg ctctgtgcag aatcctgtct atcacaatca
     8461 gcctctgaac cccgcgccca gcagagaccc acactaccag gacccccaca gcactgcagt
     8521 gggcaacccc gagtatctca acactgtcca gcccacctgt gtcaacagca cattcgacag
     8581 ccctgcccac tgggcccaga aaggcagcca ccaaattagc ctggacaacc ctgactacca
     8641 gcaggacttc tttcccaagg aagccaagcc aaatggcatc tttaagggct ccacagctga
     8701 aaatgcagaa tacctaaggg tcgcgccaca aagcagtgaa tttattggag caggatctat
     8761 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     8821 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     8881 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     8941 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     9001 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     9061 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     9121 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     9181 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     9241 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     9301 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     9361 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     9421 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagct
//
