LOCUS       sequence-49432-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  TITLE     Gutkind Lab pCEFL plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCEFL KZ HA
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             55..81
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     promoter        complement(154..172)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(155..172)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(192..209)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    198..422
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      468..848
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(555..574)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     765..786
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtgtcgtgag gattggcgaa gcttctcgag gccgccacca tgggatccac catgtacgac
       61 gttcctgatt acgctagcct cccgagatct cctgaattct gcagatatcc atcacactgg
      121 cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc
      181 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
      241 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
      301 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
      361 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
      421 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
      481 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      541 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      601 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      661 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
      721 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
      781 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
      841 gatttcggcc tattggttaa aaaatgagct gatt
//
