LOCUS       sequence-494322-        9455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFR-mEGFP in mammalian cells regulated by a synthetic 
            microRNA-based dosage compensation circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9455)
  TITLE     miRNA circuit modules for precise control of gene expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-synmiR-L-mRuby-Pro-EGFR-mEGFP-4×17
FEATURES             Location/Qualifiers
     source          1..9455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      775..1363
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1264..1283
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   1425..1554
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     polyA_signal    1569..1617
                     /note="synthetic polyadenylation signal"
     ncRNA           complement(1821..1835)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(1997..2707)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     regulatory      2704..2713
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2729..2940)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        2985..3288
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             join(3532..3603,3604..5466,5467..5535,5536..7161)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             join(7168..7170,7171..7173,7174..7887)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7213..7234)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7474..7493)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7821..7842
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7995..8116
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8032..8051)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8086..8105
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8142..8282
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   8142..8271
                     /label=AAV2 ITR
     rep_origin      8357..8812
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8444..8463)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8654..8675
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8829..8848)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8948..8970
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9008..9026)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9094..9198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9199..9267,9268..9455,1..604)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSLIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
       61 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      121 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
      181 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
      241 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
      301 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
      361 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
      421 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
      481 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
      541 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
      601 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
      661 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
      721 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      781 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      841 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      901 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
      961 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1021 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1081 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1141 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1201 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1261 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1321 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1381 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtcctgca ggcagctgcg
     1441 cgctcgctcg ctcactgagg ccgcccgggc gtcgggcgac ctttggtcgc ccggcctcag
     1501 tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt tcctgcggcc
     1561 gcacgcgtca cacaaaaaac caacacacag atctaatgaa aataaagatc ttttattatt
     1621 tcttccacaa gcctgcagga aaaagaaaga ggatgaatgg tcaggaaaag tgatttaatt
     1681 tataccattt taattcagct ttgtaaaaat gtatcaaaga gatagcaagg tattcagttt
     1741 tagtaaacaa gataattgct cgaattctag ccccttgaag tccgaggcag taggcatagg
     1801 aattataatg cttatctata catctgtggc ttcactatag ataagcatta taattcctgc
     1861 gctcactgtc aacagcaata taccttctcg agccttctgt tgggttaaga agtcgaagta
     1921 atcccagcaa gtgtttccaa gatgtgcagg caacgattct gtaaagtact gaagcctcat
     1981 tcaaacttac ctgctactta tatagctcgt ccatgccacc accaaggttg ctgtatttgg
     2041 caactgccac ttctctttgc actacgtagg tttcattgtc actctcctcg atcctttcca
     2101 ggcggtgatc aacggcatgg acaccgggca tcttgatgtt cccgacggtc ttttttgacc
     2161 tgtaagttgt cacgaagttg cagtgcagat ggccaccacc atcaactttc agtgcgatgt
     2221 cagtgtatcc tctcagacca ccatctgctg gatacatcat ctctgtatta ggctcccaac
     2281 ccttggtctt cttctgcatc acgggaccat tggagggaaa gtttacccct ctgaccttga
     2341 cgttgtagac gagctcgcca tcctcaaggc tggtgtcctg cgtgacggtg acgactccac
     2401 catcttcgta tctcgtaact ctttcccaag taaaaccctc aggaaaggac tgtttaaaga
     2461 aatcagggat gtcggccggg tacttgataa aggtacggct gccatacatg aacgacgtgg
     2521 caagaatgtc aaaggcaaat ggcaggggtc ctccctcgat gactttgatc ctcatggttt
     2581 gcactccctc gtacggtctg ccttctcctt cacctgtgca tttgaattgg tggccgttga
     2641 ccgaaccttc catgaccacc ttcatacgca tattttcctt gatcagctct tcgcccttag
     2701 acaccatggt ggcgtcgact cacgacacct gtgttctggc ggcaaacccg ttgcgaaaaa
     2761 gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc
     2821 ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg
     2881 ttcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc
     2941 actgacgggc accggagccc tatgacttgc atagctgcgt atggcgttac ataacttacg
     3001 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     3061 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     3121 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     3181 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     3241 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatgaa ttgagggcgt
     3301 caccgctaag gctccgcccc agcctgggct ccacaaccaa tgaagggtaa tctcgacaaa
     3361 gagcaagggg tggggcgcgg gcgcgcaggt gcagcagcac acaggctggt cgggagggcg
     3421 gggcgcgacg tctgccgtgc ggggtcccgg catcggttgc gcgcgcgctc cctcctctcg
     3481 gagagagggc tgtggtaaaa cccgtccgga aaccacttaa gcttcgccac catgagacct
     3541 agcgggacgg caggagctgc cctgttggcc ctattggcgg ctctttgccc agccagtcgg
     3601 gctctggagg aaaagaaagt ttgccaaggc acgagtaaca agctcacgca gttgggcact
     3661 tttgaagatc attttctcag cctccagagg atgttcaata actgtgaggt ggtccttggg
     3721 aatttggaaa ttacctatgt gcagaggaat tatgatcttt ccttcttaaa gaccatccag
     3781 gaggtggctg gttatgtcct cattgccctc aacacagtgg agcgaattcc tttggaaaac
     3841 ctgcagatca tcagaggaaa tatgtactac gaaaattcct atgccttagc agtcttatct
     3901 aactatgatg caaataaaac cggactgaag gagctgccca tgagaaattt acaggaaatc
     3961 ctgcatggcg ccgtgcggtt cagcaacaac cctgccctgt gcaacgtgga gagcatccag
     4021 tggcgggaca tagtcagcag tgactttctc agcaacatgt cgatggactt ccagaaccac
     4081 ctgggcagct gccaaaagtg tgatccaagc tgtcccaatg ggagctgctg gggtgcagga
     4141 gaggagaact gccagaaact gaccaaaatc atctgtgccc agcagtgctc cgggcgctgc
     4201 cgtggcaagt cccccagtga ctgctgccac aaccagtgtg ctgcaggctg cacaggcccc
     4261 cgggagagcg actgcctggt ctgccgcaaa ttccgagacg aagccacgtg caaggacacc
     4321 tgccccccac tcatgctcta caaccccacc acgtaccaga tggatgtgaa ccccgagggc
     4381 aaatacagct ttggtgccac ctgcgtgaag aagtgtcccc gtaattatgt ggtgacagat
     4441 cacggctcgt gcgtccgagc ctgtggggcc gacagctatg agatggagga agacggcgtc
     4501 cgcaagtgta agaagtgcga agggccttgc cgcaaagtgt gtaacggaat aggtattggt
     4561 gaatttaaag actcactctc cataaatgct acgaatatta aacacttcaa aaactgcacc
     4621 tccatcagtg gcgatctcca catcctgccg gtggcattta ggggtgactc cttcacacat
     4681 actcctcctc tggatccaca ggaactggat attctgaaaa ccgtaaagga aatcacaggg
     4741 tttttgctga ttcaggcttg gcctgaaaac aggacggacc tccatgcctt tgagaaccta
     4801 gaaatcatac gcggcaggac caagcaacat ggtcagtttt ctcttgcagt cgtcagcctg
     4861 aacataacat ccttgggatt acgctccctc aaggagataa gtgatggaga tgtgataatt
     4921 tcaggaaaca aaaatttgtg ctatgcaaat acaataaact ggaaaaaact gtttgggacc
     4981 tccggtcaga aaaccaaaat tataagcaac agaggtgaaa acagctgcaa ggccacaggc
     5041 caggtctgcc atgccttgtg ctcccccgag ggctgctggg gcccggagcc cagggactgc
     5101 gtctcttgcc ggaatgtcag ccgaggcagg gaatgcgtgg acaagtgcaa ccttctggag
     5161 ggtgagccaa gggagtttgt ggagaactct gagtgcatac agtgccaccc agagtgcctg
     5221 cctcaggcca tgaacatcac ctgcacagga cggggaccag acaactgtat ccagtgtgcc
     5281 cactacattg acggccccca ctgcgtcaag acctgcccgg caggagtcat gggagaaaac
     5341 aacaccctgg tctggaagta cgcagacgcc ggccatgtgt gccacctgtg ccatccaaac
     5401 tgcacctacg gatgcactgg gccaggtctt gaaggctgtc caacgaatgg gcctaagatc
     5461 ccgtccatcg ccactgggat ggtgggggcc ctcctcttgc tgctggtggt ggccctgggg
     5521 atcggcctct tcatgcgaag gcgccacatc gttcggaagc gcacgctgcg gaggctgctg
     5581 caggagaggg agcttgtgga gcctcttaca cccagtggag aagctcccaa ccaagctctc
     5641 ttgaggatct tgaaggaaac tgaattcaaa aagatcaaag tgctgggctc cggtgcgttc
     5701 ggcacggtgt ataagggact ctggatccca gaaggtgaga aagttaaaat tcccgtcgct
     5761 atcaaggaat taagagaagc aacatctccg aaagccaaca aggaaatcct cgatgaagcc
     5821 tacgtgatgg ccagcgtgga caacccccac gtgtgccgcc tgctgggcat ctgcctcacc
     5881 tccaccgtgc agctcatcac gcagctcatg cccttcggct gcctcctgga ctatgtccgg
     5941 gaacacaaag acaatattgg ctcccagtac ctgctcaact ggtgtgtgca gatcgcaaag
     6001 ggcatgaact acttggagga ccgtcgcttg gtgcaccgcg acctggcagc caggaacgta
     6061 ctggtgaaaa caccgcagca tgtcaagatc acagattttg ggctggccaa actgctgggt
     6121 gcggaagaga aagaatacca tgcagaagga ggcaaagtgc ctatcaagtg gatggcattg
     6181 gaatcaattt tacacagaat ctatacccac cagagtgatg tctggagcta cggggtgact
     6241 gtttgggagt tgatgacctt tggatccaag ccatatgacg gaatccctgc cagcgagatc
     6301 tcctccatcc tggagaaagg agaacgcctc cctcagccac ccatatgtac catcgatgtc
     6361 tacatgatca tggtcaagtg ctggatgata gacgcagata gtcgcccaaa gttccgtgag
     6421 ttgatcatcg aattctccaa aatggcccga gacccccagc gctaccttgt cattcagggg
     6481 gatgaaagaa tgcatttgcc aagtcctaca gactccaact tctaccgtgc cctgatggat
     6541 gaagaagaca tggacgacgt ggtggatgcc gacgagtacc tcatcccaca gcagggcttc
     6601 ttcagcagcc cctccacgtc acggactccc ctcctgagct ctctgagtgc aaccagcaac
     6661 aattccaccg tggcttgcat tgatagaaat gggctgcaaa gctgtcccat caaggaagac
     6721 agcttcttgc agcgatacag ctcagacccc acaggcgcct tgactgagga cagcatagac
     6781 gacaccttcc tcccagtgcc tgaatacata aaccagtccg ttcccaaaag gcccgctggc
     6841 tctgtgcaga atcctgtcta tcacaatcag cctctgaacc ccgcgcccag cagagaccca
     6901 cactaccagg acccccacag cactgcagtg ggcaaccccg agtatctcaa cactgtccag
     6961 cccacctgtg tcaacagcac attcgacagc cctgcccact gggcccagaa aggcagccac
     7021 caaattagcc tggacaaccc tgactaccag caggacttct ttcccaagga agccaagcca
     7081 aatggcatct ttaagggctc cacagctgaa aatgcagaat acctaagggt cgcgccacaa
     7141 agcagtgaat ttattggagc aggatctatg gtgagcaagg gcgaggagct gttcaccggg
     7201 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     7261 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     7321 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     7381 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     7441 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     7501 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     7561 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     7621 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     7681 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     7741 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
     7801 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     7861 ctcggcatgg acgagctgta caagtagagc tttactatta taatgcttat ctatactatt
     7921 ataatgctta tctatactat tataatgctt atctatacta ttataatgct tatctagggc
     7981 ccatcgatgc tagcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8041 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8101 tcatcaatgt atcttatcat gtctgcggac cgagcggccg caggaacccc tagtgatgga
     8161 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     8221 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
     8281 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     8341 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8401 acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc
     8461 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8521 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     8581 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8641 acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc
     8701 tattcttttg atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg
     8761 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     8821 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     8881 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     8941 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     9001 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     9061 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     9121 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     9181 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     9241 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     9301 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     9361 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     9421 tgtggcgcgg tattatccct tattgacgcc gggca
//
