LOCUS       sequence-49433-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Acker MG, Kolitz SE, Mitchell SF, Nanda JS, Lorsch JR
  TITLE     Reconstitution of yeast translation initiation.
  JOURNAL   Methods Enzymol. 2007;430:111-45.
  PUBMED    17913637
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2007"; volume: "430"; pages: "111-45"
COMMENT     Sequence Label: pTYB2-eIF1A
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttgtttaact ttaagaagga ganatacata tgggtaagaa aaacactaaa ggtggtaaaa
       61 aaggtagaag aggtaagaac gactctgacg gtccaaagcg tgaacttatt tataaggaag
      121 aaggccaaga atatgctcaa atcaccaaga tgttgggtaa tggaagagtc gaagccagtt
      181 gctttgatgg taataagaga atggcccata ttagaggtaa gttaagaaag aaagtctgga
      241 tgggacaagg tgatattatt cttgtttcct taagagattt ccaagatgac caatgtgatg
      301 ttgtccacaa atataattta gatgaagcca gaacactgaa aaaccaaggt gaacttcctg
      361 aaaacgccaa aattaatgaa acagacaact ttggtttcga atctgatgaa gacgttaact
      421 ttgaatttgg taacgctgat gaagatgatg aggaaggtga agatgaagaa cttgatattg
      481 atgacattcc cgggtgcttt gccaagggta ccaatgtttt aatggcggat gggtctattg
      541 aatgtattga aaacattgag gttggtaata aggtcatggg taaagatggc agacctcgtg
      601 aggtaattaa attgcccaga ggaagagaaa ctatgtacag cgtcgtgcag anngtcagca
      661 cagagcccac aaaagtgact caagtcgtga agtgccagaa ttactcaagt ttacgtgtaa
      721 tgcgacccat gagttggttg ttagaacacc tcgtagtgtc cgccgtttgt ctcgtaccat
      781 taagggtgtc gaatattttg aagttattac ttttgagatg ggccaaaaga aagcccccga
      841 cggtagaatt
//
