LOCUS       sequence-494343-        4538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3d for further cloning or expression in insect cells for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4538)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACEBac1-eIF3d-HA
FEATURES             Location/Qualifiers
     source          1..4538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1909..1935
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2095..2229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2132..2151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2186..2205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2350..2515
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2705..3293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3194..3213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  3428..3651
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3718..4251)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4440..4445,4446..4462,4463..4468))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatggc taagttcatg actccagtga tccaagacaa cccttctggt
      301 tggggtcctt gtgctgtgcc tgaacagttt agagacatgc cctaccaacc attctccaag
      361 ggtgataggc tcggtaaagt cgcagattgg actggagcca cctaccaaga caagagatac
      421 accaacaagt acagctccca gtttggagga ggaagccagt acgcttactt tcatgaggag
      481 gacgaatcct ctttccagct cgtcgatact gccaggacac agaagaccgc ttaccaacgc
      541 aacaggatga ggtttgccca gcgcaatctc aggagagaca aggacaggag gaacatgctg
      601 caattcaacc tccagatcct ccctaagagc gctaaacaga aagagagaga gaggatcagg
      661 ctgcagaaga agttccagaa acagtttgga gtgaggcaga aatgggatca gaagtcccag
      721 aagccaaggg attcctccgt tgaggtccgc agcgattggg aggttaagga agaaatggac
      781 ttccctcagc tgatgaagat gagatacctg gaagtgtccg aaccacaaga catcgagtgt
      841 tgtggtgctc tggagtacta cgataaggcc ttcgacagga ttactactcg ctccgagaag
      901 ccactgcgct ccatcaagag aatcttccac actgtgacaa caactgacga cccagtcatt
      961 cgcaagctgg ctaagactca aggaaacgtc ttcgctactg atgctatcct ggccactctg
     1021 atgagctgca ccaggtctgt ctattcctgg gacattgtgg tgcagagagt gggatctaaa
     1081 ctgttcttcg ataagaggga caattccgat ttcgatctcc tgaccgtctc cgagactgcc
     1141 aacgaaccac ctcaagatga aggcaacagc ttcaacagcc cacgcaatct ggctatggag
     1201 gctacttaca tcaaccacaa cttctcccaa cagtgtctcc gcatgggtaa ggagaggtac
     1261 aactttccaa atcccaatcc tttcgttgaa gacgatatgg acaagaacga gatcgcctcc
     1321 gttgcttatc gctatagacg ctggaagctg ggagacgaca ttgacctgat cgttaggtgc
     1381 gaacatgacg gagtgatgac aggtgcaaac ggtgaggtta gcttcatcaa catcaagaca
     1441 ctgaacgagt gggattctag gcactgcaat ggtgtggact ggagacagaa actcgatagc
     1501 cagagaggtg ctgttatcgc tacagagctg aagaacaact cctacaaact ggctcgctgg
     1561 acttgttgtg ctctgctcgc tggaagcgaa tacctgaagc tgggttatgt ttctcgctac
     1621 catgtgaagg atagcagcag acatgtgatc ctcggcaccc agcagttcaa gcctaacgag
     1681 ttcgccagcc agatcaacct cagcgtcgag aacgcttggg gtatcctgag atgcgttatc
     1741 gacatctgca tgaaactcga agaaggcaag tacctgatcc tgaaagatcc aaacaaacag
     1801 gtgattaggg tgtactctct cccagacggc accttctctt ccgatgagga cgaagaggag
     1861 gaagaagaag aggaagaaga ggaggaggaa gaggagacag gctcctccta cccttatgac
     1921 gtccctgatt acgcctaagg tacctctaga gcctgcagtc tcgacaagct tgtcgagaag
     1981 tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     2041 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     2101 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2161 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     2221 gtctggatct gatcactgct tgagcctaga agatccggct gctaacaaag cccgaaagga
     2281 agctgagttg gctgctgcca ccgctgagca ataactatca taacccctag ggtataccca
     2341 tctaattgga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc
     2401 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat
     2461 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacaaccgg
     2521 ttgacttggg tcaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2581 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2641 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2701 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2761 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2821 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2881 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2941 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3001 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3061 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3121 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3181 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3241 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3301 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3361 ctgcgttatc ccctgattga cttgggtcgc tcttcctgtg gatgcgcaga tgccctgcgt
     3421 aagcgggtgt gggcggacaa taaagtctta aactgaacaa aatagatcta aactatgaca
     3481 ataaagtctt aaactagaca gaatagttgt aaactgaaat cagtccagtt atgctgtgaa
     3541 aaagcatact ggacttttgt tatggctaaa gcaaactctt cattttctga agtgcaaatt
     3601 gcccgtcgta ttaaagaggg gcgtggccaa gggcatgtaa agactatatt cgcggcgttg
     3661 tgacaattta ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta
     3721 ggtggcggta cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt
     3781 atagagagcc actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc
     3841 aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg
     3901 gtgctcgccg gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt
     3961 gggcagaacg taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc
     4021 gatgaatgtc ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga
     4081 gtaggtggct acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt
     4141 gacttggtca gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt
     4201 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc
     4261 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca
     4321 tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac
     4381 cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca
     4441 gtttacgaac cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg
     4501 tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgcc
//
