LOCUS       sequence-494360-       10625 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector that expresses Flag-tagged SMARCAL1 with a 
            deletion of the RPA-binding domain located in the N-terminal region 
            (Delta-N).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10625)
  AUTHORS   Feng S, Liu K, Shang J, Hoeg L, Pastore G, Yang W, Roy S, 
            Sastre-Moreno G, Young JTF, Wu W, Xu D, Durocher D
  TITLE     Profound synthetic lethality between SMARCAL1 and FANCM.
  JOURNAL   Mol Cell. 2024 Dec 5;84(23):4522-4537.e7. doi: 
            10.1016/j.molcel.2024.10.016. Epub 2024 Nov 6.
  PUBMED    39510066
REFERENCE   2  (bases 1 to 10625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.10.016"; journalName: "Mol Cell"; date: 
            "2024-12-5- 5"; volume: "84"; issue: "23"; pages: "4522-4537.e7"
COMMENT     Sequence Label: pHIVT9-NAT-3xFlag-SMARCAL1-Delta-N
FEATURES             Location/Qualifiers
     source          1..10625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             119..299
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(361..949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(441..460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1120..1911,1912..1980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1743..1762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1981..2085)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2160..2179)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2351..2730
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2732..2930
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2885..2905
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2948..3128
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3175..3300
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3797..4030
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4215..4259
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4557..4674
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4737..5915
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4968..5906
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5863..5883
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5927..5992
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(8796..9370,9371..9373,9374..9382)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8963..8980)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9190..9209
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9962..10015
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
       61 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
      121 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      181 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
      241 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
      301 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      361 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      421 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      481 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      541 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      601 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      661 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      721 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      781 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      841 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      901 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      961 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1021 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1081 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1141 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1201 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1261 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1321 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1381 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1441 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1501 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1561 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1621 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1681 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1741 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1801 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1861 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1921 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1981 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2041 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2101 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     2161 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     2221 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     2281 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     2341 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2401 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2461 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2521 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2581 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2641 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2701 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2761 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2821 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2881 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
     2941 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3001 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3061 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3121 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     3181 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     3241 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     3301 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     3361 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3421 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3481 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3541 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3601 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
     3661 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     3721 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     3781 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     3841 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     3901 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     3961 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     4021 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     4081 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     4141 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     4201 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     4261 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     4321 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     4381 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     4441 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     4501 acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt
     4561 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     4621 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     4681 tattacaggg acagcagaga tccagtttgg ttagtaccgg gcccgctcta gcgtgaggct
     4741 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     4801 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     4861 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtacccc cgtgcagtag
     4921 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     4981 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     5041 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     5101 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
     5161 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     5221 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     5281 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     5341 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     5401 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     5461 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     5521 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     5581 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     5641 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     5701 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     5761 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     5821 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     5881 ttcaaagttt ttttcttcca tttcaggtgt cgtgagcggc cgcatggact acaaagacca
     5941 tgacggtgat tataaagatc atgacatcga ctacaaagac gatgacgaca aggctagcgg
     6001 tagcggtagc catcagagga ctagctcggg cacctccatt gctggcaacc cattccaggc
     6061 caagcaaggc ccatcccaaa atttcccaag ggagtcttgt aagccagtga gccatggtgt
     6121 cattttcaag caacagaatc tcagtagctc atctaatgct gaccaaagac ctcatgattc
     6181 ccacagtttt caggcaaagg gaatatggaa aaagccagaa gaaatgccca cagcctgccc
     6241 aggccacagt ccacgtagtc aaatggctct cactggaatc tctcctccct tggcacaaag
     6301 tcctccagag gtccctaaac aacagctctt gagttatgag ttaggtcaag gtcatgctca
     6361 ggcttcacct gagatcaggt tcacaccctt tgctaaccca actcataagc ctctggccaa
     6421 accaaagagt tcccaagaga caccagctca ttcctctgga cagcctccca gggatgctaa
     6481 gttagaggcc aagacagcaa aagcctcccc ttcggggcag aacatttctt acatccattc
     6541 tagctcagag agtgtaacgc ccaggacaga aggaagactc cagcagaagt cagggtcctc
     6601 agtccaaaaa ggagtgaact ctcagaaggg aaagtgcgta aggaacggcg atcgtttcca
     6661 ggtgttgatt gggtacaatg cagaactcat tgcagtgttt aagaccctgc ccagcaagaa
     6721 ttatgatcct gacaccaaga cgtggaactt cagcatgaat gactatagtg ccctgatgaa
     6781 agcagcccag agcctcccca cggtcaacct gcagcctctg gaatgggcct atggcagcag
     6841 cgagtcaccc tccaccagca gtgagggaca ggccggcctt ccatcagctc catccctttc
     6901 atttgtcaaa gggcgatgca tgctcatctc cagggcctac ttcgaggcag acatcagtta
     6961 ttcacaggac cttattgcgc tttttaaaca gatggattcc agaagatatg atgtcaagac
     7021 caggaagtgg agctttctct tggaagagca cagtaaacta attgcaaagg tgcgctgcct
     7081 cccacaagtt cagctggacc ctctgcccac gactctcacc ctggcgtttg cttctcagct
     7141 caagaagaca tctctcagtc tcacgccaga tgtcccagag gcagaccttt ctgaagtgga
     7201 ccccaagctc gtgtctaatc tgatgccctt tcagagagct ggagtcaatt ttgccatagc
     7261 caaaggaggc cgcctgctgc tcgctgacga catgggcctg gggaagacca tccaagccat
     7321 ctgcatcgca gccttttacc ggaaggagtg gccgctcctg gtggtggtgc catcctccgt
     7381 gcgcttcacc tgggagcagg ccttccttcg gtggctgcca tctctgagcc cagattgcat
     7441 caacgtcgtg gtgactggga aggaccgcct gacagctggc ctgatcaaca ttgtcagctt
     7501 tgaccttctt agcaagttgg aaaaacagct aaaaacccct tttaaagttg tcatcattga
     7561 tgaatctcac ttcctcaaaa acagtaggac tgcccgctgt cgagcagcta tgccggtcct
     7621 aaaggttgcc aagagggtga tcctgttgtc gggcacacca gccatgtccc ggcccgcaga
     7681 gctctacacg cagatcatcg cagtcaagcc aactttcttc ccccagtttc atgcctttgg
     7741 acttcgctac tgtgatgcca aacggatgcc ttgggggtgg gactactcag gttcctccaa
     7801 cctgggagag ctgaagctcc tgctggagga agcagtcatg ctgcggcgcc tcaagtccga
     7861 cgtcctttcc cagctgcctg ccaagcagcg caagatagtg gtgattgccc caggacggat
     7921 caatgccagg accagagctg ccctggatgc tgcagccaag gaaatgacca ccaaggacaa
     7981 aactaaacag cagcagaaag atgccctcat tctcttcttc aacagaacag ctgaagctaa
     8041 aatcccatct gtcattgaat atatcttgga cctactggaa agtggaagag agaagttttt
     8101 agtatttgca caccataagg tggtcctgga cgcaattacg caagagcttg agagaaagca
     8161 cgtgcagcac atccgcatcg atggctccac ctcatcagct gagcgggagg acctgtgcca
     8221 gcagttccaa ctgtcggaga ggcatgctgt ggccgtgctg tccatcaccg ctgccaatat
     8281 gggcctcacc ttctcctcgg ctgacctggt ggtgtttgct gagctgtttt ggaacccagg
     8341 ggtgctgatc caggctgagg accgcgtgca ccgcattgga cagaccagct ccgtgggcat
     8401 tcactacctc gtggcaaagg gcacagctga tgactacctt tggcccctga ttcaagagaa
     8461 gattaaagtt ctggcagaag ccgggctttc tgagaccaat ttttcagaaa tgacagaatc
     8521 cactgattac ctctacaagg acccaaagca gcagaagatc tacgacctat tccagaagtc
     8581 ctttgagaaa gaaggaagtg atatggagct cctggaagca gcagagtcct ttgacccagg
     8641 aagtgcttca ggaacatctg gaagtagttc ccagaacatg ggagacaccc tggatgaaag
     8701 ctcattgaca gccagtccac agaagaaaag gagatttgaa ttttttgata actgggacag
     8761 ctttacgtct cccctgtaac ccgggctagg atccgcccct ctccctcccc cccccctaac
     8821 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     8881 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     8941 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     9001 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     9061 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     9121 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     9181 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     9241 ccccattgta tgggatctga tctggggcct cggtacacat gctttacatg tgtttagtcg
     9301 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     9361 cgatgataat atggccacaa ccatgggcac caccctggac gacacggctt accgataccg
     9421 caccagtgtc cctggggacg ccgaggccat cgaggcactg gatgggtcct tcaccaccga
     9481 caccgtcttc cgcgtcaccg ccaccgggga cggcttcacc ctgcgggagg tgccggtgga
     9541 cccgcccctg accaaggtgt tccccgacga cgaatcggac gacgaatcgg acgacgggga
     9601 ggacggcgac ccggactccc ggacgttcgt cgcgtacggg gacgacggcg acctggcggg
     9661 cttcgtggtc gtctcgtact ccggctggaa ccgccggctg accgtcgagg acattgaggt
     9721 agcacccgag cacaggggac acggggtggg acgggcattg atgggactgg ctaccgagtt
     9781 cgccagagaa cggggagctg gtcatctttg gctcgaagtc accaacgtca acgcaccggc
     9841 gatccacgct taccggcgga tggggttcac cctctgcggc ctggacaccg ccctgtacga
     9901 cggcaccgcc tcggacggcg agcaggcgct ctacatgtcc atgccctgcc ccggcagtgg
     9961 cgagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaatgaa
    10021 gcgcttcctc ttcctcctac tcaccatcag cctcctggtt atggtacaga tacaaactgg
    10081 actctcagga caaaacgaca ccagccaaac cagcagcccc tcagcatcca gcaacataag
    10141 cggaggcatt ttccttttct tcgtggctaa tgccataatc cacctcttct gcttcagttg
    10201 aggcgcgcca aatttatcga taccgtcgac ttcgatcgag acctagaaaa acatggagca
    10261 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
    10321 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
    10381 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
    10441 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    10501 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
    10561 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
    10621 cgctt
//
