LOCUS       sequence-494368-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty lentiviral backbone for generation of ClonMapper Duo Green 
            (H2B-GFP) gRNA barcode library for use with polyA capture or gRNA 
            capture scRNA-seq technologies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  TITLE     Mapping cell-cell fusion at single-cell resolution
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CMDuoGreen_Crop-Seq-H2B-GFP-WPRE-TS-hU6-BsmbI
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     275..294
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     528..545
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    662..683
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(698..703,704..721,722..728)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    736..752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     741..763
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     760..776
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     760..776
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     795..815
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        797..815
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        843..1069
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1070..1250
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1297..1422
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1915..2148
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2333..2377
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2558..2675
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2814..3992
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3045..3983
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3940..3960
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3994..4371
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4384..4393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4390..4392,4393..4395,4396..5109)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4390..4392,4393..4395,4396..5106)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4435..4456)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4696..4715)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5043..5064
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5120..5708
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5173..5193)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5591..5602)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5901..6149
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5901..5921
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6072..6091
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8031..8106
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     LTR             8159..8339
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8417..8538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8454..8473)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8508..8527
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8578..8713
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8733..8752)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8734..8752)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8762..8779)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8762..8778)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8771..8793)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8920..9375
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9007..9026)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9217..9238
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9401..9505
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9506..9574,9575..10366)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9724..9743)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10537..10751,1..374)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
       61 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      121 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
      181 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      241 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      301 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
      361 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
      421 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
      481 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
      541 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      601 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      661 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      721 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      781 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
      841 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
      901 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      961 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     1021 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     1081 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1141 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1201 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     1261 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     1321 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1381 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1441 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1501 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1561 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1621 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1681 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1741 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1801 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1861 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1921 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1981 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     2041 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     2101 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     2161 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     2221 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2281 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2341 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2401 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2461 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2521 tcagaatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     2581 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     2641 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     2701 atccactttg gcgccggctc gagggggccc gggtgcaaag atggataaag ttttaaacag
     2761 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg
     2821 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     2881 tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     2941 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     3001 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     3061 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     3121 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     3181 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     3241 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     3301 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     3361 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     3421 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     3481 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     3541 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     3601 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     3661 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     3721 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     3781 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     3841 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     3901 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     3961 aaagtttttt tcttccattt caggtgtcgt gacatgccag agccagcgaa gtctgctccc
     4021 gccccgaaaa agggctccaa gaaggcggtg actaaggcgc agaagaaagg cggcaagaag
     4081 cgcaagcgca gccgcaagga gagctattcc atctatgtgt acaaggttct gaagcaggtc
     4141 caccctgaca ccggcatttc gtccaaggcc atgggcatca tgaattcgtt tgtgaacgac
     4201 attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa gcgctcgacc
     4261 atcacctcca gggagatcca gacggccgtg cgcctgctgc tgcctgggga gttggccaag
     4321 cacgccgtgt ccgagggtac taaggccatc accaagtaca ccagcgctaa ggatccaccg
     4381 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     4441 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     4501 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     4561 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     4621 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4681 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     4741 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4801 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     4861 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     4921 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4981 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     5041 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     5101 tacaagtaat taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     5161 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     5221 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     5281 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     5341 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     5401 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5461 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     5521 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     5581 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     5641 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     5701 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     5761 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatttcacc
     5821 attatcgttt cagacccacc tcccaacccc gaggggaccc aagcagtggt atcaacgcag
     5881 agtacatggg gggacccaga gagggcctat ttcccatgat tccttcatat ttgcatatac
     5941 gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag
     6001 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     6061 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
     6121 tatatatctt gtggaaagga cgaaacaccg gagacggttg taaatgagca cacaaaatac
     6181 acatgctaaa atattatatt ctatgacctt tataaaatca accaaaatct tctttttaat
     6241 aactttagta tcaataatta gaatttttat gttccttttt gcaaactttt aataaaaatg
     6301 agcaaaataa aaaaacgcta gttttagtaa ctcgcgttgt tttcttcacc tttaataata
     6361 gctactccac cacttgttcc taagcggtca gctcctgctt caatcatttt ttgagcatct
     6421 tcaaatgttc taactccacc agctgcttta actaaagcat tgtctttaac aactgacttc
     6481 attagtttaa catcttcaaa tgttgcacct gattttgaaa atcctgttga tgttttaaca
     6541 aattctaatc cagcttcaac agctatttca caagctttca tgatttcttc ttttgttaat
     6601 aaacaatttt ccataataca tttaacaaca tgtgatccag ctgctttttt tacagctttc
     6661 atgtcttcta aaactaattc ataatttttg tcttttaatg caccaatatt taataccata
     6721 tcaatttctg ttgcaccatc tttaattgct tcagaaactt cgaatgcttt tgtagctgtt
     6781 gtgcatgcac ctagaggaaa acctacaaca tttgttattc ctacatttgt gccttttaat
     6841 aattctttac aatagcttgt tcaatatgaa ttaacacaaa ctgttgcaaa atcaaattca
     6901 attgcttcat cacataattg tttaatttca gctttcgtag catcttgttt taataatgtg
     6961 tgatctatat atttgtttag tttcattttt tctcctatat attcattttt aattttaatt
     7021 ctttaataat ttcgtctact ttaactttag cgttttgaac agattcacca acacctataa
     7081 aataaatttt tagtttaggt tcagttccac ttgggcgaac agcaaatcat gacttatctt
     7141 ctaaataaaa ttttagtaag tcttgtcctg gcatattata cattccatcg atgtagtctt
     7201 caacattaac aactttaagt ccagcaattt gagttaaggg tgttgctctc aatgatttca
     7261 ttaatggttc aatttttaat ttcttttctt ctggtttaaa attcaagttt aaagtgaaag
     7321 tgtaatatgc acccatttct ttaaataaat cttctaaata gtctactaat gttttatttt
     7381 gttttttata aaatcaagca gcctctgcta ttaatataga agcttgtatt ccatctttat
     7441 ctctagctga gtcatcaatt acatatccat aactttcttc ataagcaaaa acaaaattta
     7501 atccgttatc ttcttcttta gcaatttctc tacccattca tttaaatcca gttaaagttt
     7561 ttacaatatt aactccatat ttttcatgag cgattctatc acccaaatca cttgttacaa
     7621 aacttgaata tagagccgga ttttttggaa tgctatttaa gcgttttaga tttgataatt
     7681 ttcaatcaat taaaattggt cctgtttgat ttccatctaa tcttacaaaa tgaccatcat
     7741 gttttattgc cattccaaat ctgtcagcat ctgggtcatt cataataata atatctgcat
     7801 catgtttaat accatattca agcggtattt ttcatgcagg atcaaattct ggatttggat
     7861 ttacaacatt tttaaatgtt tcatcttcaa atgcatgctc ttcaacctca ataacgttat
     7921 atcctgattc acgtaatatt tttggggtaa atttagttcc tgttccatta actgcgctaa
     7981 aaataatttt taaatctttt ttagcttctt gctctttttt gtacgtctct gttttagagc
     8041 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     8101 cggtgctttt ttaagcttgg cgtaactaga tcttgagaca ctgctttttg cttgtactgg
     8161 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8221 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8281 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8341 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     8401 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8461 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8521 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     8581 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8641 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8701 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     8761 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     8821 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     8881 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     8941 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     9001 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     9061 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     9121 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     9181 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     9241 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     9301 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     9361 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     9421 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     9481 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     9541 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     9601 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     9661 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     9721 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     9781 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     9841 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     9901 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     9961 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    10021 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    10081 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    10141 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    10201 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    10261 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    10321 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    10381 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    10441 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10501 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10561 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10621 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10681 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10741 atacctcgct c
//
