LOCUS       sequence-494408-       11814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid expresses the full length BRCA1 sequence (G1788V 
            mutation) with an N-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11814)
  AUTHORS   Gracia B, Zhang XH, Montes P, Pham TC, Huang M, Chen J, Karras GI
  TITLE     HSP90 buffers deleterious genetic variations in BRCA1.
  JOURNAL   Mol Cell. 2025 Nov 19:S1097-2765(25)00867-6. doi: 
            10.1016/j.molcel.2025.10.026.
  PUBMED    41265458
REFERENCE   2  (bases 1 to 11814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Nov 19:S1097-2765(25)00867-6. doi: 
            10.1016/j.molcel.2025.10.026."
COMMENT     Sequence Label: pKlab816-bGlobin_3xF-BRCA1(full-length)-G1788V
FEATURES             Location/Qualifiers
     source          1..11814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        347..550
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     501..521
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     595..614
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        595..613
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          650..1222
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1319..1328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1328..1393
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1412..1453
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    1451..1475
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(7099..7117)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7100..7117)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7137..7154)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7143..7367
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7413..7841
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7500..7519)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7710..7731
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7850..7870)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7855..8184
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8035..8170
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8097..8116
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8251..9045
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8305..8324)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8915..8934
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9219..9340
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9256..9275)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9310..9329
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9389..9405)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9389..9405)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9402..9424)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9413..9429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9437..9443,9444..9461,9462..9467))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9482..9503
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9620..9637)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9791..10379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9871..9890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10550..11341,11342..11410))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11173..11192
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11411..11515)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11590..11609)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(11781..11814,1..346)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
       61 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      121 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      181 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      241 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      301 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      361 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      421 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      481 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      541 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
      601 gactcactat agggagaccc aagctagagt cgatcctgag aacttcaggg tgagtttggg
      661 gacccttgat tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa
      721 agttttcagg gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg
      781 ataattttgt ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt
      841 ttcattttct gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt
      901 cacttttgtt tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa
      961 tcagggtata ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg
     1021 ataaggtaga atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag
     1081 aaacaactac atcctggtca tcatcctgcc tttctcttta tggttacaat gatatacact
     1141 gtttgagatg aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca
     1201 tgccttcttc tttttcctac agctcctggg caacgtgctg gtttgtgctg tcgaccccaa
     1261 gcttggccgc cagtgtgctg gaattctgca gatatccatc acactggcgg ccgctcgagc
     1321 caccatggac tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga
     1381 tgacgatgac aagggctcgg gctcgacctc gggtaagcct atccctaacc ctctcctcgg
     1441 tctcgattct acaagtttgt acaaaaaagt tggcgattta tctgctcttc gcgttgaaga
     1501 agtacaaaat gtcattaatg ctatgcagaa aatcttagag tgtcccatct gtctggagtt
     1561 gatcaaggaa cctgtctcca caaagtgtga ccacatattt tgcaaatttt gcatgctgaa
     1621 acttctcaac cagaagaaag ggccttcaca gtgtccttta tgtaagaatg atataaccaa
     1681 aaggagccta caagaaagta cgagatttag tcaacttgtt gaagagctat tgaaaatcat
     1741 ttgtgctttt cagcttgaca caggtttgga gtatgcaaac agctataatt ttgcaaaaaa
     1801 ggaaaataac tctcctgaac atctaaaaga tgaagtttct atcatccaaa gtatgggcta
     1861 cagaaaccgt gccaaaagac ttctacagag tgaacccgaa aatccttcct tgcaggaaac
     1921 cagtctcagt gtccaactct ctaaccttgg aactgtgaga actctgagga caaagcagcg
     1981 gatacaacct caaaagacgt ctgtctacat tgaattggga tctgattctt ctgaagatac
     2041 cgttaataag gcaacttatt gcagtgtggg agatcaagaa ttgttacaaa tcacccctca
     2101 aggaaccagg gatgaaatca gtttggattc tgcaaaaaag gctgcttgtg aattttctga
     2161 gacggatgta acaaatactg aacatcatca acccagtaat aatgatttga acaccactga
     2221 gaagcgtgca gctgagaggc atccagaaaa gtatcagggt agttctgttt caaacttgca
     2281 tgtggagcca tgtggcacaa atactcatgc cagctcatta cagcatgaga acagcagttt
     2341 attactcact aaagacagaa tgaatgtaga aaaggctgaa ttctgtaata aaagcaaaca
     2401 gcctggctta gcaaggagcc aacataacag atgggctgga agtaaggaaa catgtaatga
     2461 taggcggact cccagcacag aaaaaaaggt agatctgaat gctgatcccc tgtgtgagag
     2521 aaaagaatgg aataagcaga aactgccatg ctcagagaat cctagagata ctgaagatgt
     2581 tccttggata acactaaata gcagcattca gaaagttaat gagtggtttt ccagaagtga
     2641 tgaactgtta ggttctgatg actcacatga tggggagtct gaatcaaatg ccaaagtagc
     2701 tgatgtattg gacgttctaa atgaggtaga tgaatattct ggttcttcag agaaaataga
     2761 cttactggcc agtgatcctc atgaggcttt aatatgtaaa agtgaaagag ttcactccaa
     2821 atcagtagag agtaatattg aagacaaaat atttgggaaa acctatcgga agaaggcaag
     2881 cctccccaac ttaagccatg taactgaaaa tctaattata ggagcatttg ttactgagcc
     2941 acagataata caagagcgtc ccctcacaaa taaattaaag cgtaaaagga gacctacatc
     3001 aggccttcat cctgaggatt ttatcaagaa agcagatttg gcagttcaaa agactcctga
     3061 aatgataaat cagggaacta accaaacgga gcagaatggt caagtgatga atattactaa
     3121 tagtggtcat gagaataaaa caaaaggtga ttctattcag aatgagaaaa atcctaaccc
     3181 aatagaatca ctcgaaaaag aatctgcttt caaaacgaaa gctgaaccta taagcagcag
     3241 tataagcaat atggaactcg aattaaatat ccacaattca aaagcaccta aaaagaatag
     3301 gctgaggagg aagtcttcta ccaggcatat tcatgcgctt gaactagtag tcagtagaaa
     3361 tctaagccca cctaattgta ctgaattgca aattgatagt tgttctagca gtgaagagat
     3421 aaagaaaaaa aagtacaacc aaatgccagt caggcacagc agaaacctac aactcatgga
     3481 aggtaaagaa cctgcaactg gagccaagaa gagtaacaag ccaaatgaac agacaagtaa
     3541 aagacatgac agtgatactt tcccagagct gaagttaaca aatgcacctg gttcttttac
     3601 taagtgttca aataccagtg aacttaaaga atttgtcaat cctagccttc caagagaaga
     3661 aaaagaagag aaactagaaa cagttaaagt gtctaataat gctgaagacc ccaaagatct
     3721 catgttaagt ggagaaaggg ttttgcaaac tgaaagatct gtagagagta gcagtatttc
     3781 actggtacct ggtactgatt atggcactca ggaaagtatc tcgttactgg aagttagcac
     3841 tctagggaag gcaaaaacag aaccaaataa atgtgtgagt cagtgtgcag catttgaaaa
     3901 ccccaaggga ctaattcatg gttgttccaa agataataga aatgacacag aaggctttaa
     3961 gtatccattg ggacatgaag ttaaccacag tcgggaaaca agcatagaaa tggaagaaag
     4021 tgaacttgat gctcagtatt tgcagaatac attcaaggtt tcaaagcgcc agtcatttgc
     4081 tctgttttca aatccaggaa atgcagaaga ggaatgtgca acattctctg cccactctgg
     4141 gtccttaaag aaacaaagtc caaaagtcac ttttgaatgt gaacaaaagg aagaaaatca
     4201 aggaaagaat gagtctaata tcaagcctgt acagacagtt aatatcactg caggctttcc
     4261 tgtggttggt cagaaagata agccagttga taatgccaaa tgtagtatca aaggaggctc
     4321 taggttttgt ctatcatctc agttcagagg caacgaaact ggactcatta ctccaaataa
     4381 acatggactt ttacaaaacc catatcgtat accaccactt tttcccatca agtcatttgt
     4441 taaaactaaa tgtaagaaaa atctgctaga ggaaaacttt gaggaacatt caatgtcacc
     4501 tgaaagagaa atgggaaatg agaacattcc aagtacagtg agcacaatta gccgtaataa
     4561 cattagagaa aatgttttta aaggagccag ctcaagcaat attaatgaag taggttccag
     4621 tactaatgaa gtgggctcca gtattaatga aataggttcc agtgatgaaa acattcaagc
     4681 agaactaggt agaaacagag ggccaaaatt gaatgctatg cttagattag gggttttgca
     4741 acctgaggtc tataaacaaa gtcttcctgg aagtaattgt aagcatcctg aaataaaaaa
     4801 gcaagaatat gaagaagtag ttcagactgt taatacagat ttctctccat atctgatttc
     4861 agataactta gaacagccta tgggaagtag tcatgcatct caggtttgtt ctgagacacc
     4921 tgatgacctg ttagatgatg gtgaaataaa ggaagatact agttttgctg aaaatgacat
     4981 taaggaaagt tctgctgttt ttagcaaaag cgtccagaga ggagagctta gcaggagtcc
     5041 tagccctttc acccatacac atttggctca gggttaccga agaggggcca agaaattaga
     5101 gtcctcagaa gagaacttat ctagtgagga tgaagagctt ccctgcttcc aacacttgtt
     5161 atttggtaaa gtaaacaata taccttctca gtctactagg catagcaccg ttgctaccga
     5221 gtgtctgtct aagaacacag aggagaattt attatcattg aagaatagct taaatgactg
     5281 cagtaaccag gtaatattgg caaaggcatc tcaggaacat caccttagtg aggaaacaaa
     5341 atgttctgct agcttgtttt cttcacagtg cagtgaattg gaagacttga ctgcaaatac
     5401 aaacacccag gatcctttct tgattggttc ttccaaacaa atgaggcatc agtctgaaag
     5461 ccagggagtt ggtctgagtg acaaggaatt ggtttcagat gatgaagaaa gaggaacggg
     5521 cttggaagaa aataatcaag aagagcaaag catggattca aacttaggtg aagcagcatc
     5581 tgggtgtgag agtgaaacaa gcgtctctga agactgctca gggctatcct ctcagagtga
     5641 cattttaacc actcagcaga gggataccat gcaacataac ctgataaagc tccagcagga
     5701 aatggctgaa ctagaagctg tgttagaaca gcatgggagc cagccttcta acagctaccc
     5761 ttccatcata agtgactcct ctgcccttga ggacctgcga aatccagaac aaagcacatc
     5821 agaaaaagca gtattaactt cacagaaaag tagtgaatac cctataagcc agaatccaga
     5881 aggcctttct gctgacaagt ttgaggtgtc tgcagatagt tctaccagta aaaataaaga
     5941 accaggagtg gaaaggtcat ccccttctaa atgcccatca ttagatgata ggtggtacat
     6001 gcacagttgc tctgggagtc ttcagaatag aaactaccca tctcaagagg agctcattaa
     6061 ggttgttgat gtggaggagc aacagctgga agagtctggg ccacacgatt tgacggaaac
     6121 atcttacttg ccaaggcaag atctagaggg aaccccttac ctggaatctg gaatcagcct
     6181 cttctctgat gaccctgaat ctgatccttc tgaagacaga gccccagagt cagctcgtgt
     6241 tggcaacata ccatcttcaa cctctgcatt gaaagttccc caattgaaag ttgcagaatc
     6301 tgcccagggt ccagctgctg ctcatactac tgatactgct gggtataatg caatggaaga
     6361 aagtgtgagc agggagaagc cagaattgac agcttcaaca gaaagggtca acaaaagaat
     6421 gtccatggtg gtgtctggcc tgaccccaga agaatttatg ctcgtgtaca agtttgccag
     6481 aaaacaccac atcactttaa ctaatctaat tactgaagag actactcatg ttgttatgaa
     6541 aacagatgct gagtttgtgt gtgaacggac actgaaatat tttctaggaa ttgcgggagg
     6601 aaaatgggta gttagctatt tctgggtgac ccagtctatt aaagaaagaa aaatgctgaa
     6661 tgagcatgat tttgaagtca gaggagatgt ggtcaatgga agaaaccacc aaggtccaaa
     6721 gcgagcaaga gaatcccagg acagaaagat cttcaggggg ctagaaatct gttgctatgg
     6781 gcccttcacc aacatgccca cagatcaact ggaatggatg gtacagctgt gtgttgcttc
     6841 tgtggtgaag gagctttcat cattcaccct tggcacaggt gtccacccaa ttgtggttgt
     6901 gcagccagat gcctggacag aggacaatgg cttccatgca attgggcaga tgtgtgaggc
     6961 acctgtggtg acccgagagt gggtgttgga cagtgtagca ctctaccagt gccaggagct
     7021 ggacacctac ctgatacccc agatccccca cagccactac tgagatatcg gctcgagcat
     7081 gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc
     7141 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     7201 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     7261 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     7321 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     7381 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     7441 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     7501 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     7561 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     7621 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     7681 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     7741 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     7801 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     7861 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7921 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7981 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     8041 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8101 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     8161 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     8221 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     8281 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     8341 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     8401 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     8461 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     8521 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     8581 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     8641 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     8701 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     8761 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     8821 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     8881 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     8941 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     9001 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     9061 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     9121 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     9181 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     9241 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9301 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     9361 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     9421 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     9481 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     9541 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     9601 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     9661 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     9721 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     9781 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     9841 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     9901 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9961 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    10021 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    10081 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    10141 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    10201 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    10261 agccagttac cttcggaaaa agagtcggta gctcttgatc cggcaaacaa accaccgctg
    10321 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    10381 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    10441 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    10501 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    10561 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    10621 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    10681 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    10741 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    10801 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    10861 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    10921 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10981 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    11041 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    11101 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    11161 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    11221 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    11281 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    11341 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    11401 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    11461 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    11521 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
    11581 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
    11641 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
    11701 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
    11761 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatc
//
