LOCUS       sequence-494425-        9507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid expresses the BACH1 protein sequence for pull down 
            assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Gracia B, Zhang XH, Montes P, Pham TC, Huang M, Chen J, Karras GI
  TITLE     HSP90 buffers deleterious genetic variations in BRCA1.
  JOURNAL   Mol Cell. 2025 Nov 19:S1097-2765(25)00867-6. doi: 
            10.1016/j.molcel.2025.10.026.
  PUBMED    41265458
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Nov 19:S1097-2765(25)00867-6. doi: 
            10.1016/j.molcel.2025.10.026."
COMMENT     Sequence Label: pKlab1194-pcDNA-3.1-BACH1/BRIP1/FANCJ-3xFLAG-V5
FEATURES             Location/Qualifiers
     source          1..9507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1211..1230)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1402..1781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1782..1985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1936..1956
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2030..2049
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2030..2048
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2136..2160
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5911..5935)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5937..6002
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6021..6062
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6106..6123)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6112..6223
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6372..6800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6459..6478)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6669..6690
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6809..6829)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6814..7143
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6994..7129
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7056..7075
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7192..8217
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7192..8217
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8347..8468
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8384..8403)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8438..8457
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8517..8533)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8517..8533)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8530..8552)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8541..8557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8565..8571,8572..8589,8590..8595))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8610..8631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8748..8765)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8919..9507)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8999..9018)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     1381 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     1441 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1501 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1561 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1621 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     1681 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     1741 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     1801 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1861 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     1921 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1981 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
     2041 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
     2101 cactagtcca gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagttggca
     2161 ccatgtcttc aatgtggtct gaatatacaa ttggtggggt gaagatttac tttccttata
     2221 aagcttaccc gtcacagctt gctatgatga attctattct cagaggatta aacagcaagc
     2281 aacattgttt gttggagagt cccacaggaa gtggaaaaag cttagcctta ctttgttctg
     2341 ctttagcatg gcaacaatct cttagtggga aaccagcaga tgagggcgta agtgaaaaag
     2401 ctgaagtaca attgtcatgt tgttgtgcat gccattcaaa ggattttaca aacaatgaca
     2461 tgaaccaagg aacttcacgt catttcaact atccaagcac accaccttct gaaagaaatg
     2521 gcacttcatc aacttgtcaa gactcccctg aaaaaaccac tctggctgca aagttatctg
     2581 ctaagaaaca ggcatccata tacagagatg aaaatgatga ttttcaagta gagaagaaaa
     2641 gaattcgacc cttagaaact acacagcaga ttagaaaacg tcattgcttt ggaacagaag
     2701 tacacaattt ggatgcaaaa gttgattcag gaaagactgt aaaactcaac tctccactgg
     2761 aaaagataaa ctccttttcg ccacagaaac cccctggcca ctgttctagg tgctgttgtt
     2821 ctactaaaca aggaaacagt caagagtcat cgaataccat taagaaggat catacaggga
     2881 aatccaagat acccaaaata tattttggga cacgcacaca caagcagatt gctcagatta
     2941 ctagagagct ccggaggacg gcatattcag gggttccaat gactattctt tccagcaggg
     3001 atcatacttg tgtccatcct gaggtagtcg gtaacttcaa cagaaatgag aagtgcatgg
     3061 aattgctaga tgggaaaaac ggaaaatcct gctattttta tcatggagtt cataaaatta
     3121 gtgatcagca cacattacag actttccaag ggatgtgcaa agcctgggat atagaagaac
     3181 ttgtcagcct ggggaagaaa ctaaaggcct gtccatatta cacagcccga gaactaatac
     3241 aagatgctga catcatattt tgtccctaca actatcttct agatgcacaa ataagggaaa
     3301 gtatggattt aaatctgaaa gaacaggttg tcattttaga tgaagctcat aacatcgagg
     3361 actgtgctcg ggaatcagca agttacagtg taacagaagt tcagcttcgg tttgctcggg
     3421 atgaactaga tagtatggtc aacaataata taaggaagaa agatcatgaa cccctacgag
     3481 ctgtgtgctg tagcctcatt aattggttag aagcaaacgc tgaatatctt gtagaaagag
     3541 attatgaatc agcttgtaaa atatggagtg gaaatgaaat gctcttaact ttacacaaaa
     3601 tgggtatcac cactgctact tttcccattt tgcagggaca tttttctgct gttcttcaaa
     3661 aagaggaaaa aatctcacca atttatggta aagaggaggc aagagaagta cctgttatta
     3721 gtgcatcaac tcaaataatg cttaaaggac tttttatggt acttgactat ctttttaggc
     3781 aaaatagcag atttgcagat gattataaaa ttgcgattca acagacttac tcctggacaa
     3841 atcagattga tatttcagac aaaaatgggt tgttggttct accaaaaaat aagaaacgtt
     3901 cacgacagaa aactgcagtt catgtgctaa acttttggtg cttaaatcca gctgtggcct
     3961 tttcagatat taatggcaaa gttcagacca ttgttttgac atctggtaca ttatcaccaa
     4021 tgaaatcctt ttcgtcagaa cttggtgtta catttactat ccagctggag gctaatcata
     4081 tcattaaaaa ttcacaggtt tgggttggta ccattgggtc aggccccaag ggtcggaatc
     4141 tctgtgctac cttccagaat actgaaacat ttgagttcca agatgaagtg ggagcacttt
     4201 tgttatctgt gtgccagact gtgagccaag gaattttgtg tttcttgcca tcttacaagt
     4261 tattagaaaa attaaaagaa cgttggctct ctactggttt atggcataat ctggagttgg
     4321 tgaagacagt cattgtagaa ccacagggag gagaaaaaac aaattttgat gaattactgc
     4381 aggtgtacta tgacgcaatc aaatacaaag gagagaaaga tggagctctc ctggtagcag
     4441 tttgtcgtgg taaagtgagt gagggtctgg atttctcaga tgacaatgcc cgtgctgtca
     4501 taacaatagg aattcctttt ccaaatgtga aagatctaca ggttgaacta aaacgacaat
     4561 acaatgacca ccattcaaaa ttgagaggtc ttctacctgg ccgtcagtgg tatgaaattc
     4621 aagcatacag ggccttaaac caggcccttg gtagatgtat tagacacaga aatgattggg
     4681 gagctcttat tctagtggat gatcgcttta ggaataaccc aagtcgctat atatctggac
     4741 tttctaaatg ggtacggcag cagattcagc accattcaac ctttgaaagt gcactggagt
     4801 ccttggctga attttccaaa aagcatcaaa aagttcttaa tgtatccata aaggacagaa
     4861 ccaatataca ggacaatgag tctacacttg aagtgacctc tttaaagtac agtaccccac
     4921 cttatttact ggaagcagca agtcatctat caccagaaaa ttttgtggaa gatgaagcaa
     4981 agatatgtgt ccaggaacta cagtgtccta aaattattac caaaaattca cctctaccaa
     5041 gtagcattat ctccagaaag gagaaaaatg atccagtatt cctggaagaa gcagggaaag
     5101 cagaaaaaat tgtgatttcc agatccacaa gcccaacttt caacaaacaa acaaagagag
     5161 ttagctggtc aagctttaat tctttgggac agtattttac tggtaaaata ccgaaggcaa
     5221 cacctgagct cgggtcatca gagaatagtg cctctagtcc tccccgtttc aaaacagaga
     5281 agatggaaag taaaactgtt ttgcccttca ctgataaatg tgaatcctca aatctgacag
     5341 taaacacatc gtttggatca tgccctcaat cagaaaccat tatttcatca ttaaagattg
     5401 atgccaccct tactagaaaa aatcattctg aacatccgct ctgttctgaa gaagccctgg
     5461 atccagacat tgaattgtct ctagtaagtg aagaagataa acagtccact tcaaatagag
     5521 attttgaaac agaagcagaa gatgaatcta tctattttac acctgaactt tacgatcctg
     5581 aagatacaga tgaagaaaaa aatgacctag ctgaaactga tagaggaaat agattggcta
     5641 acaattcaga ttgcatttta gctaaagacc tttttgaaat tagaactata aaagaagtag
     5701 attcagccag agaagtgaaa gctgaggatt gcatagatac aaagttgaat ggaattctgc
     5761 atattgaaga aagtaaaatt gatgacattg atggtaatgt aaaaacaact tggataaatg
     5821 aactggaact gggaaaaact catgaaatag aaataaagaa ctttaaacca tctccttcca
     5881 aaaataaagg catgtttcct ggttttaagt tgccaacttt cttgtacaaa gtggttgatt
     5941 acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca
     6001 agggctcggg ctcgacctcg ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     6061 catgataact cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     6121 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6181 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     6241 gtcattctat tctggggggt ggggtggggc aggacagcaa ggggaagaca atagcaggca
     6301 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     6361 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6421 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6481 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6541 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6601 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6661 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6721 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6781 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     6841 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     6901 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6961 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7021 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7081 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     7141 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gatgaaaaag
     7201 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     7261 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     7321 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     7381 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     7441 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     7501 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     7561 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     7621 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     7681 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     7741 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     7801 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     7861 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     7921 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     7981 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     8041 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     8101 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     8161 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac
     8221 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8281 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8341 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8401 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8461 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     8521 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8581 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8641 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8701 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8761 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8821 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8881 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8941 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9001 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9061 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9121 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9181 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9241 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9301 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9361 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9421 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9481 attacgcgca gaaaaaaagg atctcaa
//
