LOCUS       sequence-494428-        9279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid expresses the hPALB2 protein seqence for pull down 
            assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Gracia B, Zhang XH, Montes P, Pham TC, Huang M, Chen J, Karras GI
  TITLE     HSP90 buffers deleterious genetic variations in BRCA1.
  JOURNAL   Mol Cell. 2025 Nov 19:S1097-2765(25)00867-6. doi: 
            10.1016/j.molcel.2025.10.026.
  PUBMED    41265458
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Nov 19:S1097-2765(25)00867-6. doi: 
            10.1016/j.molcel.2025.10.026."
COMMENT     Sequence Label: pKlab1197-pcDNA-3.1-3xFLAG-V5-hPALB2 (T333A, L...
FEATURES             Location/Qualifiers
     source          1..9279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1211..1230)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1402..1781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1782..1985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1936..1956
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1982..2006
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2075..2140
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2159..2200
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2198..2222
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5793..5817)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5868..5885)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5874..6098
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6144..6572
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6231..6250)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6441..6462
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6581..6601)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6586..6915
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6766..6901
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6828..6847
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6964..7989
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6964..7989
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8119..8240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8156..8175)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8210..8229
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8289..8305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8289..8305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8302..8324)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8313..8329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8337..8343,8344..8361,8362..8367))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8382..8403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8520..8537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8691..9279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8771..8790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     1381 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     1441 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1501 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1561 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1621 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     1681 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     1741 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     1801 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1861 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     1921 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1981 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
     2041 atagaagaca ccgactctag aggatccagc catggactac aaagaccatg acggtgatta
     2101 taaagatcat gacatcgact acaaggatga cgatgacaag ggctcgggct cgacctcggg
     2161 taagcctatc cctaaccctc tcctcggtct cgattctaca agtttgtaca aaaaagcagg
     2221 ctccaccatg gacgagcctc ccgggaagcc cctcagctgt gaggagaagg aaaagttaaa
     2281 ggagaaatta gcattcttga aaagggaata cagcaagaca ctagcccgcc ttcagcgtgc
     2341 ccaaagagct gaaaagatta agcattctat taagaaaaca gtagaagaac aagattgttt
     2401 gtctcagcag gatctctcac cgcagctaaa acactcagaa cctaaaaata aaatatgtgt
     2461 ttatgacaag ttacacatca aaacccatct tgatgaagaa actggagaaa agacatctat
     2521 cacacttgat gttgggcctg agtcctttaa ccctggagat ggcccaggag gattacctat
     2581 acaaagaaca gatgacaccc aagaacattt tccccacagg gtcagtgacc ctagtggtga
     2641 gcaaaagcag aagctgccaa gcagaagaaa gaagcagcag aagaggacat ttatttcaca
     2701 ggagagagac tgtgtctttg gcactgattc actcagattg tctgggaaaa gactaaagga
     2761 acaggaagaa atcagtagca aaaatcctgc tagatcacca gtaactgaaa taagaactca
     2821 ccttttaagt cttaaatctg aacttccaga ttctccagaa ccagttacag aaattaatga
     2881 agacagtgta ttaattccac caactgccca accagaaaaa ggtgttgata cattcctaag
     2941 aagacctaat ttcaccaggg cgactacagt tcctttacag actctatcag atagcggtag
     3001 tagtcagcac cttgaacaca ttcctcctaa aggtagcagt gaacttacta ctcacgacct
     3061 aaaaaacatt agatttactt cacctgtaag tttggaggca caaggcaaaa aaatgactgt
     3121 ctctacagat aacctccttg taaataaagc tataagtaaa agtggccaac tgcccacaag
     3181 ttctaattta gaggcaaata tttcatgttc tctaaatgaa ctcgcctaca ataacttacc
     3241 agcaaatgaa aaccaaaact taaaagaaca aaatcaaaca gagaaatctt taaaatctcc
     3301 cagtgacact cttgatggca ggaatgaaaa tcttcaggaa agtgagattc taagtcaacc
     3361 taagagtctt agcctggaag caacctctcc tctttctgca gaaaaacatt cttgcacagt
     3421 gcctgaaggc cttctgtttc ctgcagaata ttatgttaga acaacacgaa gcatgtccaa
     3481 ttgccagagg aaagtagccg tggaggctgt cattcagagt catttggatg tcaagaaaaa
     3541 agggtttaaa aataaaaata aggatgcaag taaaaattta aacctttcca atgaggaaac
     3601 tgaccaaagt gaaattagga tgtctggcac atgcacagga caaccaagtt caagaacctc
     3661 tcagaaactt ctctcattaa ctaaagtcag ctctcccgct gggcccactg aagataatga
     3721 cttgtctagg aaggcagttg cccaagcacc tggtagaaga tacacaggaa aaagaaaatc
     3781 agcctgcacc ccagcatcag atcattgtga accacttttg ccaacttcta gcctgtcgat
     3841 tgttaacagg tccaaggaag aagtcacctc acacaaatat cagcacgaaa aattatttat
     3901 tcaagtgaaa gggaagaaaa gtcgtcatca aaaagaggat tccctttctt ggagtaatag
     3961 tgcttattta tccttggatg atgatgcttt cacggctcca tttcataggg atggaatgct
     4021 gagtttaaag caactactgt cttttctcag tatcacagac tttcagttac ctgatgaaga
     4081 ctttggacct cttaagcttg aaaaagtgaa gtcctgctca gaaaaaccag tggagccctt
     4141 tgagtcaaaa atgtttggag agagacatct taaagaggga agctgtattt ttccagagga
     4201 actgagtcct aaacgcatgg atacagaaat ggaggactta gaagaggacc ttattgttct
     4261 accaggaaaa tcacatccca aaaggccaaa ctcgcaaagc cagcatacaa agacgggcct
     4321 ttcttcatcc atactacttt atactccttt aaatacggtt gcgcctgatg ataatgacag
     4381 gcctaccaca gacatgtgtt cacctgcttt ccccatctta ggtactactc cagcctttgg
     4441 ccctcaaggc tcctatgaaa aagcatctac agaagttgct ggacgaactt gctgcacacc
     4501 ccaacttgct catttgaaag actcagtctg tcttgccagt gatactaaac aattcgacag
     4561 ttcaggcagc ccagcaaaac cacataccac cctgcaagtg tcaggcaggc aaggacaacc
     4621 tacctgtgac tgtgactctg tcccgccagg aacacctcca cccattgagt cattcacttt
     4681 taaagaaaat cagctctgta gaaacacatg ccaggagctg cataaacatt ccgtcgaaca
     4741 gactgaaaca gcagagcttc ctgcttctga tagcataaac ccaggcaacc tacaattggt
     4801 ttcagagtta aagaatcctt caggttcctg ttccgtagat gtgagtgcca tgttttggga
     4861 aagagccggt tgtaaagagc catgtatcat aactgcttgc gaagatgtag tttctctttg
     4921 gaaagctctg gatgcttggc agtgggaaaa actttatacc tggcacttcg cagaggttcc
     4981 agtattacag atagttccag tgcctgatgt gtataatctc gtgtgtgtag ctttgggaaa
     5041 tttggaaatc agagagatca gggcattgtt ttgttcctct gatgatgaaa gtgaaaagca
     5101 agtactactg aagtctggaa atataaaagc tgtgcttggc ctgacaaaga ggaggctagt
     5161 tagtagcagt gggacccttt ctgatcaaca agtagaagtc atgacgtttg cagaagatgg
     5221 aggaggcaaa gaaaaccaat ttttgatgcc ccctgaggag actatactaa cttttgctga
     5281 ggtccaaggg atgcaagaag ctctgcttgg tactactatt atgaacaaca ttgttatttg
     5341 gaatttaaaa actggtcaac tcctgaaaaa gatgcacatt gatgattctt accaagcttc
     5401 agtctgtcac aaagcctatt ctgaaatggg gcttctcttt attgtcctga gtcatccctg
     5461 tgccaaagag agtgagtcgt tgcgaagccc tgtgtttcag ctcattgtga ttaaccctaa
     5521 gacgactctc agcgtgggtg tgatgctgta ctgtcttcct ccagggcagg ctggcaggtt
     5581 cctggaaggt gacgtgaaag atcactgtgc agcagcaatc ttgacttctg gaacaattgc
     5641 catttgggac ttacttctcg gtcagtgtac tgccctcctc ccacctgtct ctgaccaaca
     5701 ttggtctttt gtgaaatggt cgggtacaga ctctcatttg ctggctggac aaaaagatgg
     5761 aaatatattt gtataccgct attcaaagct tgacccagct ttcttgtaca aagtggttgc
     5821 tctatagtaa ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     5881 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5941 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     6001 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     6061 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     6121 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6181 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6241 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6301 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6361 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6421 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6481 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6541 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     6601 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6661 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6721 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     6781 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6841 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6901 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     6961 gtgatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     7021 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     7081 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     7141 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     7201 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     7261 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     7321 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     7381 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     7441 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     7501 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     7561 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     7621 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     7681 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     7741 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     7801 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     7861 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     7921 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     7981 aaggaatagc acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     8041 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     8101 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8161 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8221 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     8281 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8341 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8401 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8461 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8521 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8581 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8641 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8701 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8761 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8821 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8881 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8941 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9001 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9061 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaa
//
