LOCUS       sequence-494494-        9302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for human RECK, N-terminal 3xFLAG-BAP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9302)
  AUTHORS   Heiden R, Hannig L, Kuo CJ, Ergun S, Braunger BM, Vallon M
  TITLE     WNT7A/B assemble a GPR124-RECK-LRP5/6 co-receptor complex to 
            activate beta-catenin signaling in brain endothelial cells.
  JOURNAL   J Biol Chem. 2025 Sep 4:110682. doi: 10.1016/j.jbc.2025.110682.
  PUBMED    40914247
REFERENCE   2  (bases 1 to 9302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2025 Sep 4:110682. doi: 10.1016/j.jbc.2025.110682."
COMMENT     Sequence Label: p3xFLAG-CMV-9-BAP-RECK
FEATURES             Location/Qualifiers
     source          1..9302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             932..976
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             977..1042
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1055..1099
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     polyA_signal    4043..4665
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4242..4261)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4689..4709)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4707..5005
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5059..5853
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5113..5132)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5723..5742
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6505..6639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6542..6561)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6596..6615
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6644..6663)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6645..6663)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6677..6693)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6677..6693)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6690..6712)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6701..6717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6725..6731,6732..6749,6750..6755))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6770..6791
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6908..6925)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7079..7667)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7159..7178)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7838..8629,8630..8698))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8461..8480
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8699..8803)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8917..8936)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9144..9165
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaattcac catgtctgca cttctgatcc tagctcttgt
      961 tggagctgca gttgctgact acaaagacca tgacggtgat tataaagatc atgacatcga
     1021 ttacaaggat gacgatgaca agcttgcggc cgctggtctg aacgacatct tcgaggctca
     1081 gaaaatcgaa tggcacgagg cggccgctga ggtggcaggg ggcctggctc cgggcagtgc
     1141 gggtgcattg tgttgtaatc attcaaagga taaccaaatg tgccgtgatg tatgtgaaca
     1201 gattttctcc tcaaaaagtg aatcccgact aaaacatctg ttgcagcgag ccccagatta
     1261 ttgcccagag acaatggttg aaatttggaa ttgtatgaat tcatctttgc caggtgtgtt
     1321 taagaagtct gatggctggg ttggcttagg ctgctgtgaa ctggctattg ccttggagtg
     1381 tcgacaggca tgcaagcagg catcttcaaa gaatgatatt tccaaagttt gcagaaaaga
     1441 atatgagaat gctcttttca gttgcattag cagaaatgaa atgggctcgg tttgttgcag
     1501 ttatgcaggt catcacacaa actgccgaga atactgtcaa gccatttttc gaacagactc
     1561 ttctcctggt ccatctcaga taaaagcagt ggaaaattat tgcgcctcta ttagtccaca
     1621 attaatacat tgtgtgaaca attatactca atcttatcca atgaggaacc caacggatag
     1681 tttatattgc tgtgacagag ctgaagacca tgcttgccaa aatgcctgca agagaatcct
     1741 gatgtccaag aaaacggaaa tggagattgt tgatggtctc atcgagggtt gtaagaccca
     1801 gcccttgcct caagatcctc tttggcaatg ttttcttgaa agctcacaat ctgttcaccc
     1861 tggagtcact gtacaccctc ctccctctac aggcctcgat ggggctaaat tgcattgttg
     1921 ttctaaagca aacacttcaa catgtaggga actctgcact aaactttaca gcatgagctg
     1981 gggcaataca cagagttggc aagagtttga tcgcttttgt gaatataatc cagtggaagt
     2041 gtccatgttg acctgtttag cggatgtccg ggaaccttgc cagttgggct gtagaaacct
     2101 tacttactgt actaatttta acaacaggcc aacagaactt ttcaggagtt gtaatgcaca
     2161 gtcagatcaa ggagccatga atgacatgaa gttgtgggag aaaggaagca taaagatgcc
     2221 atttatcaat atacctgttc ttgatattaa aaagtgccag ccagagatgt ggaaagcaat
     2281 agcttgttca ctgcagatta aaccttgtca tagtaaatct cggggaagta ttatttgcaa
     2341 atcagattgt gtggagattc ttaaaaaatg tggagaccag aacaaattcc ctgaagacca
     2401 cacagctgaa agtatttgtg agcttctgtc acctacagat gatctgaaga attgtatacc
     2461 tttggataca tacctcaggc caagtacttt aggtaacatt gtagaagaag tgactcatcc
     2521 ctgtaaccca aatccttgcc ctgccaatga gctctgtgaa gtaaaccgaa aaggatgtcc
     2581 atctggagat ccctgtcttc catacttttg tgttcaaggt tgcaaactgg gagaagcttc
     2641 tgatttcatt gtccgtcaag ggacactaat ccaggtgcca tcatctgcag gggaagttgg
     2701 ttgttataaa atctgttcat gtggacaaag tggactctta gaaaactgta tggaaatgca
     2761 ctgtatagac ctccagaagt cttgtattgt tggaggaaaa agaaaaagtc atggaacatc
     2821 ctttagtatt gactgcaatg tctgttcttg ttttgctggc aatttggtgt gctctacccg
     2881 cctttgcctc agtgagcaca gttcagaaga tgaccgtcgt accttcacag gtctgccctg
     2941 taactgtgca gatcagtttg tccctgtatg tgggcagaat gggcgcactt accccagtgc
     3001 ctgcattgct cgctgtgtgg gcctccaaga ccatcagttt gagtttggat catgcatgtc
     3061 aaaggatcca tgtaatccta atccctgcca aaaaaaccaa aggtgcatac ccaaaccaca
     3121 ggtctgcctg acgacttttg ataaatttgg atgtagccag tatgagtgtg taccaagaca
     3181 gctcgcgtgt gaccaggtcc aagatcctgt ttgtgacaca gaccacatgg agcacaacaa
     3241 tctctgcact ttataccaaa gaggaaaaag cctctcttac aaaggtccct gccagccctt
     3301 ttgcagagca accgagcccg tatgtgggca caatggtgag acctacagca gtgtgtgtgc
     3361 tgcctactcg gatcgcgtgg cagtcgatta ctatggggac tgccaggccg tcggagtcct
     3421 ctcagagcac agctccgtcg ccgagtgtgc ttctgtcaag tgtccttcgc tcttggcagc
     3481 tggatgcaaa cccatcatcc caccgggtgc ttgttgccca ttatgtgctg ggatgttaag
     3541 agttttattt gacaaagaaa aactggatac tattgctaag gtaacaaata aaaagccaat
     3601 aacagttctg gaaatacttc agaaaatccg catgcacgtg tctgtcccac agtgtgatgt
     3661 gtttggatac ttcagcattg aatcagaaat tgtgatcctg atcattcccg tcgatcacta
     3721 tccaaaagct ctgcagattg aagcctgcaa taaagaagca gagaagattg agtcccttat
     3781 caactctgac agcccgactt tggcgtccca tgtccctctc tctgccctca tcatttccca
     3841 ggtacaggtc tccagcagtg tgccatcggc cggtgtcagg gccaggcctt cttgccactc
     3901 cctcctcctt cccctcagct tgggccttgc cttgcacttg ctctggacat ataactgact
     3961 gcccacggaa agtgcagaat gctcctccac ctcactctcc tgccagatct gatatcggta
     4021 ccagtcgact ctagaggatc ccgggtggca tccctgtgac ccctccccag tgcctctcct
     4081 ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat
     4141 cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag
     4201 caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg
     4261 gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct
     4321 cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt
     4381 tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta
     4441 atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac
     4501 tgctcccttc cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac
     4561 acagcatagg ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac
     4621 tgtcctctca tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc
     4681 ttggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4741 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4801 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4861 cctaactccg cccatcccgc ctccgctaac tccgcccagt tccgcccatt ctccgcccca
     4921 tggctgacta atttttttta tttatgcagg ccgaggccgc ctcggcctct gagctattcc
     4981 agaagtagtg aggaggcttt tttggaggaa ttgatcagct tgggatctga tcaagagaca
     5041 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     5101 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     5161 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     5221 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     5281 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     5341 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     5401 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     5461 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     5521 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5581 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     5641 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5701 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5761 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5821 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     5881 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     5941 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     6001 tcatgctgga gttcttcgcc caccccgggc tcgatcccct cgcgagttgg ttcagctgct
     6061 gcctgaggct ggacgacctc gcggagttct accggcagtg caaatccgtc ggcatccagg
     6121 aaaccagcag cggctatccg cgcatccatg cccccgaact gcaggagtgg ggaggcacga
     6181 tggccgcttt ggtcgacccg gacgggacgc tcctgcgcct gatacagaac gaattgcttg
     6241 caggcatctc atgagtgtgt cttcccgttt tccgcctgag gtcactgcgt ggatggagcg
     6301 ctggcgcctg ctgcgcgacg gcgagctgct caccacccac tcgccaagct ggaaccgtaa
     6361 aaaggccgcg ttgctggcgt ttttccatag gctccgccga tcataatcag ccataccaca
     6421 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     6481 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     6541 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6601 ttgtccaaac tcatcaatgt atcttatcat gtctggatca attccctata gtgagtcgta
     6661 ttaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6721 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6781 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6841 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6901 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6961 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7021 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7081 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7141 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7201 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7261 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7321 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7381 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7441 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7501 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7561 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7621 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7681 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7741 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7801 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7861 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7921 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7981 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8041 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8101 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8161 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8221 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8281 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8341 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8401 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     8461 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8521 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8581 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8641 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     8701 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8761 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8821 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     8881 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     8941 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     9001 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     9061 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     9121 ttaagagtcc actattaaag aacgtggact cttgttccaa actggaacaa cactcaaccc
     9181 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     9241 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     9301 tt
//
