LOCUS       sequence-49457-e         325 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Mitchell SF, Walker SE, Algire MA, Park EH, Hinnebusch AG, Lorsch JR
  TITLE     The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to 
            stimulate canonical translation initiation and to block an 
            alternative pathway.
  JOURNAL   Mol Cell. 2010 Sep 24;39(6):950-62.
  PUBMED    20864040
REFERENCE   2  (bases 1 to 325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2010-09-24"; volume: "39(6)"; pages: "950-62"
COMMENT     Sequence Label: pET15b-eIF4E-S200C
FEATURES             Location/Qualifiers
     source          1..325
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    70..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      121 taagaaggag atataccatg tccgttgaag aagttagcaa gaagtttgaa gaaaacgttt
      181 cagtcgatga taccacagct actccaaaga ctgttttaag tgacagtgct cacttcgatg
      241 tcaagcaccc attgaacacc aaatgacttt atggtacaca aagccagccg tcgataaatc
      301 tgagtcgtgg tctgatctat tacgt
//
