LOCUS       sequence-49458-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Mitchell SF, Walker SE, Algire MA, Park EH, Hinnebusch AG, Lorsch JR
  TITLE     The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to 
            stimulate canonical translation initiation and to block an 
            alternative pathway.
  JOURNAL   Mol Cell. 2010 Sep 24;39(6):950-62.
  PUBMED    20864040
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2010-09-24"; volume: "39(6)"; pages: "950-62"
COMMENT     Sequence Label: pET21c-eIF4A
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    65..89
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             104..126
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaattaata cgactcacta
       61 taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga
      121 aggagatata catatgtctg aaggtattac tgatattgaa gaatcccaaa ttcaaaccaa
      181 ctatgacaag gttgtctaca agttcgatga tatggaattg gacgaaaact tgttaagagg
      241 tgttttcggt tacggtttcg aagaaccatc tgccattcaa caacgtgcca tcatgcctat
      301 tattgaaggt cacgatgtct tggctcaagc tcaatctggt actggtaaga ccggtacctt
      361 ttccattgct gctttgcaaa gaattgacac ctctgtcaag gctcctcaag ctttgatgtt
      421 ggctccaact agagaattgg ctttgcaaat ccaaaaggtt gtcatggctt tggctttcca
      481 catggacatc aaggtccacg cttgtatcgg tggtacttcc tttgttgaag acgctgaagg
      541 tttgagagat gctcaaatcg tcgttggtac tccaggtcgt gtttttgaca acatccaaag
      601 acgtagattc agaactgaca agatcaagat gttcatctta gatgaagctg atgaaatgtt
      661 gtcttctggt ttcaaggaac aaatctacca aattttcacc ttacttccac caaccactca
      721 agttgttcta ttgtccgcca ccatgccaaa tgacgtcttg gaagttacca ccaaatttat
      781 gagaaaccca gttagaattt tggttaagaa ggatgaattg
//
