LOCUS       sequence-494580-        4238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Young JA, Sermwittayawong D, Kim HJ, Nandu S, An N, 
            Erdjument-Bromage H, Tempst P, Coscoy L, Winoto A
  TITLE     Fas-associated death domain (FADD) and the E3 ubiquitin-protein 
            ligase TRIM21 interact to negatively regulate virus-induced 
            interferon production.
  JOURNAL   J Biol Chem. 2011 Feb 25;286(8):6521-31. Epub 2010 Dec 23.
  PUBMED    21183682
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-02-25"; volume: "286(8)"; pages: "6521-31. Epub 
            2010 Dec 23"
COMMENT     Sequence Label: pCI-His-hUbi
FEATURES             Location/Qualifiers
     source          1..4238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          91..223
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     206..225
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        268..286
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             295..312
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             313..537
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             313..537
                     /codon_start=1
                     /gene="Homo sapiens UBC"
                     /product="human ubiquitin C"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     polyA_signal    586..707
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(597..616)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     651..670
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      888..1343
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(975..994)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1185..1206
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1410..1429)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1529..1551
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1589..1607)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1675..1779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1780..1848,1849..2640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1998..2017)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2811..3399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3300..3319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3610..3989
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3990..4193
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4144..4164
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4190..4214
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tatcacagtt aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc
       61 tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa
      121 ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca
      181 cctattggtc ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc
      241 aattacagct cttaaggcta gagtacttaa tacgactcac tataggctag catgcaccac
      301 catcaccatc atcagatctt cgtgaagacc ctgactggta agaccatcac tctcgaagtg
      361 gagccgagtg acaccattga gaatgtcaag gcaaagatcc aagacaagga aggcatccct
      421 cctgaccagc agaggttgat ctttgctggg aaacagctgg aagatggacg caccctgtct
      481 gactacaaca tccagaaaga gtccaccctg cacctggtac tccgtctcag aggtgggtga
      541 ggtacctcta gagtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg
      601 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      661 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
      721 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa
      781 acctctacaa atgtggtaaa atcgataagg atccgggctg gcgtaatagc gaagaggccc
      841 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag
      901 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      961 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     1021 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     1081 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1141 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1201 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1261 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     1321 caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
     1381 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1441 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     1501 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     1561 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     1621 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1681 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1741 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     1801 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1861 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1921 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1981 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2041 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2101 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2161 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2221 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2281 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     2341 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2401 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2461 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     2521 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2581 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2641 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2701 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2761 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2821 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2881 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2941 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3001 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3061 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3121 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3181 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3241 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3301 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3361 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3421 ttacggttcc tggccttttg ctggcctttt gctcacatgg ctcgacagat cttcaatatt
     3481 ggccattagc catattattc attggttata tagcataaat caatattggc tattggccat
     3541 tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac
     3601 cgccatgttg gcattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     3661 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     3721 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     3781 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     3841 cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat
     3901 ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca
     3961 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc
     4021 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     4081 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt
     4141 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     4201 tgaaccgtca gatcactaga agctttattg cggtagtt
//
