LOCUS       sequence-494594-        9741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3k, eIF3l, eIF3e and eIF3e subunits for further cloning or 
            expression in insect cells for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9741)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 9741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBIG1A-eIF3klef
FEATURES             Location/Qualifiers
     source          1..9741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(110..115,116..132,133..138))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(209..242)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(288..307)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    332..380
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        475..566
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    1433..1567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1723..1814
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    3719..3853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4009..4100
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    5648..5782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5938..6029
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    7313..7447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  7761..7926
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      8116..8704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8605..8624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  8839..9062
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(9129..9662)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
       61 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
      121 cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
      181 ggcaaccttg ggcagcagcg aagtcgccat aacttcgtat agcatacatt atacgaagtt
      241 atctgggttt aaacgctcta tggtctaaag tttttatcga ggctaactga aacacggaag
      301 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
      361 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcaa
      421 gtgaacgctc tatggtctaa agatttaaat cgacctactc cggaatatta atagatcatg
      481 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
      541 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
      601 gcggatccct cgagtcaaca tggctatgtt tgagcaaatg agagccaatg ttggaaagct
      661 gctgaagggc attgacaggt acaatcccga gaatctggcc actctcgaac gctatgtgga
      721 aacacaagcc aaggagaatg cttacgacct ggaggctaat ctggctgtgc tgaaactcta
      781 ccaattcaac cctgcattct ttcagacaac cgtgactgct cagatcctcc tgaaagccct
      841 gacaaacctg cctcacaccg acttcactct ctgcaagtgc atgatcgacc aagcccacca
      901 agaagaaagg ccaatcagac aaatcctcta cctgggtgat ctcctcgaaa catgccattt
      961 ccaggccttc tggcaagccc tggacgagaa catggatctc ctcgaaggta tcactggctt
     1021 cgaagatagc gtgcgcaagt tcatctgcca tgtggtggga atcacctacc agcacatcga
     1081 ccgctggctg ctggctgaaa tgctgggaga tctgagcgat tcccagctca aggtgtggat
     1141 gtccaagtac ggatggtccg ctgatgagag cggtcagatc ttcatctgta gccaagaaga
     1201 gtctatcaaa ccaaagaaca tcgtcgagaa gattgatttc gatagcgtgt cttccatcat
     1261 ggcttccagc cagtaaggta cctctagagc ctgcagtctc gacaagcttg tcgagaagta
     1321 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     1381 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     1441 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     1501 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1561 ctggatctga tcactgcttg agcctagaag atccggctgc taacaaagcc cgaaaggaag
     1621 ctgagttggc tgctgccacc gctgagcaat aactatcata accatttaaa taaactggat
     1681 actattgcac gtttaaatcg acctactccg gaatattaat agatcatgga gataattaaa
     1741 atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa
     1801 aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc ggatccctcg
     1861 agtcaacatg tcctatcctg ccgatgacta tgaatctgag gctgcttatg atccatacgc
     1921 ctacccttct gattacgaca tgcacacagg cgaccctaaa caagatctgg catacgagag
     1981 acaatacgaa cagcaaacct accaagtgat cccagaggtg atcaagaact tcattcaata
     2041 cttccataag accgtgtccg acctcatcga ccagaaggtc tatgagctgc aggcaagcag
     2101 agtgtcttcc gacgtcattg accagaaggt gtatgagatc caggacatct atgagaacag
     2161 ctggaccaaa ctgactgaaa gattcttcaa gaacactccc tggcctgagg ctgaagctat
     2221 cgctccacag gtgggaaatg atgccgtgtt cctgattctg tacaaggaac tgtactacag
     2281 acatatctac gctaaggttt ctggaggacc ctccctggaa caaagatttg agagctacta
     2341 caactactgc aatctgttca actacattct gaatgccgat ggacctgcac ctctggaact
     2401 gcccaaccag tggctctggg acatcattga tgagttcatc tatcagttcc agtccttctc
     2461 tcagtacaga tgtaagaccg ctaagaaatc cgaagaggag attgatttcc tgagaagcaa
     2521 tcccaagatc tggaatgttc actctgtgct caacgtgctg cactccctgg tggacaaatc
     2581 caacatcaac aggcagctgg aagtgtacac aagcggtggt gatcctgaga gcgttgctgg
     2641 tgagtacggt agacatagcc tctacaagat gctgggctac ttctccctgg tgggtctgct
     2701 caggctccac tctctgctcg gagactacta ccaggctatc aaagtgctgg agaacatcga
     2761 actcaacaag aagtctatgt atagcagagt ccctgagtgc caggtcacta cttactacta
     2821 cgtgggcttc gcctacctca tgatgagacg ctaccaggat gccatcaggg tcttcgctaa
     2881 catcctcctg tacatccaga ggaccaaatc catgtttcaa aggaccactt acaagtatga
     2941 aatgatcaac aagcagaacg aacagatgca cgcactcctg gcaattgccc tgactatgta
     3001 tcccatgaga attgatgaat ccatccacct ccaactgaga gagaagtacg gagataagat
     3061 gctcaggatg cagaagggtg atcctcaggt ttacgaagaa ctgttcagct attcttgccc
     3121 aaagtttctc tctccagtcg tccctaacta tgacaacgtg cacccaaact atcacaaaga
     3181 gcctttcctg caacagctga aagtgttctc tgacgaggtc cagcagcagg ctcaactgtc
     3241 tacaatccgc agcttcctga aactctatac aactatgcct gtggctaagc tggcaggatt
     3301 cctggatctg actgaacagg agtttcgcat tcagctgctc gtgttcaagc acaagatgaa
     3361 gaacctggtg tggacttccg gaatcagcgc tctggacggt gagtttcagt ctgcaagcga
     3421 ggttgacttc tacatcgaca aggacatgat ccacatcgct gatacaaagg tggccaggag
     3481 gtacggagac ttcttcatca ggcagattca caagtttgaa gagctgaacc gcacactcaa
     3541 gaagatggga caaagaccat aaggtacctc tagagcctgc agtctcgaca agcttgtcga
     3601 gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     3661 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     3721 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3781 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3841 tcatgtctgg atctgatcac tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa
     3901 aggaagctga gttggctgct gccaccgctg agcaataact atcataacca tttaaataaa
     3961 ctggatacta ttgcacgttt aaatcgacct actccggaat attaatagat catggagata
     4021 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
     4081 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggcgcggat
     4141 ccctcgagtc aacatggctg agtacgatct gacaaccagg attgctcatt tcctggatag
     4201 gcacctggtg tttcctctcc tggagttcct ctccgtgaag gagatctaca atgagaagga
     4261 actcctccag ggtaaactgg atctgctgag cgacaccaac atggtcgatt tcgcaatgga
     4321 tgtgtacaag aacctgtaca gcgacgacat tcctcacgct ctcagggaga agaggactac
     4381 agttgttgct cagctgaagc aactgcaagc cgagactgag cccatcgtga agatgtttga
     4441 agaccctgaa acaaccaggc agatgcagtc tactagagac ggacgcatgc tgtttgacta
     4501 tctggcagac aaacacggct tcaggcagga gtacctcgac accctgtaca ggtacgctaa
     4561 gttccagtac gagtgtggca actactctgg agctgcagaa tacctgtact tcttcagagt
     4621 gctggtgcca gccacagaca ggaacgctct gtcttctctg tggggtaagc tggcttccga
     4681 aatcctgatg cagaactggg atgctgcaat ggaggacctc accagactca aagaaaccat
     4741 tgacaacaat agcgtgtctt ctccactgca gtccctccag caaagaacct ggctcatcca
     4801 ttggtccctg ttcgtgttct tcaaccatcc caagggaagg gacaacatca ttgatctgtt
     4861 tctgtaccaa cctcaatacc tgaacgccat ccagactatg tgcccacaca tcctgagata
     4921 cctgacaacc gctgtgatta ccaacaagga cgtgaggaag agaagacagg tcctcaagga
     4981 cctggtcaag gtgattcagc aggagtccta tacatacaag gacccaatca ctgagtttgt
     5041 ggaatgcctg tatgtgaact tcgacttcga cggtgctcag aagaagctga gagagtgtga
     5101 gtctgttctc gtgaacgatt tcttcctggt tgcctgcctg gaggacttca tcgagaatgc
     5161 caggctcttc atcttcgaaa ccttctgcag gattcatcag tgcattagca tcaacatgct
     5221 ggctgataag ctcaacatga cacctgaaga ggctgagaga tggatcgtga atctgattcg
     5281 caacgctagg ctcgacgcta agatcgactc taagctcggt cacgtggtga tgggcaacaa
     5341 tgccgtgtct ccataccaac aggttatcga gaagaccaag tccctgagct ttcgctctca
     5401 gatgctcgcc atgaacatcg agaagaagct gaaccagaac tccagatccg aagcacctaa
     5461 ctgggctaca caagatagcg gtttctatta aggtacctct agagcctgca gtctcgacaa
     5521 gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag aggttttact
     5581 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     5641 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5701 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5761 tgtatcttat catgtctgga tctgatcact gcttgagcct agaagatccg gctgctaaca
     5821 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta tcataaccat
     5881 ttaaataaac tggatactat tgcacgttta aatcgaccta ctccggaata ttaatagatc
     5941 atggagataa ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta
     6001 acagttttgt aataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg
     6061 ggcgcggatc cctcgagtca acatggcaac accagccgtt cctgtgtctg ctccaccagc
     6121 tactccaact cccgttcctg cagcagctcc agccagcgtt ccagctccta ctcctgctcc
     6181 tgctgcagct cccgtccctg ctgctgcacc tgcaagctct tccgaccctg ctgctgctgc
     6241 tgctgcaact gctgcaccag gtcagacacc agcttccgct caggctcctg cacagacacc
     6301 agctccagca ctgcctggac cagctctgcc aggacctttc ccaggaggaa gggtggttcg
     6361 cctgcatcct gttatcctgg cttccattgt cgatagctac gagagaagaa acgaaggtgc
     6421 tgctagggtg attggtactc tcctcggtac agttgacaag cattctgtcg aggtgactaa
     6481 ctgtttctct gtgccacaca atgaaagcga agacgaggtt gccgtggaca tggagttcgc
     6541 aaagaacatg tacgaactgc ataagaaagt tagccctaat gaactgatcc tcggttggta
     6601 cgccactgga cacgacatca ctgagcactc cgtgctcatt catgaatact actccagaga
     6661 ggctccaaat cctatccacc tgacagtgga cacctccctg cagaacggtc gcatgagcat
     6721 caaggcctac gtgtctacac tgatgggagt tccaggcagg acaatgggtg tgatgttcac
     6781 tccactgaca gtgaagtacg cttactatga caccgaaagg attggcgtgg acctgatcat
     6841 gaagacttgc ttcagcccaa acagagtcat cggactctcc tctgatctgc agcaagttgg
     6901 tggagcctct gccagaatcc aggacgcact ctctaccgtc ctgcagtatg ccgaggatgt
     6961 gctgtccggt aaggtgtccg cagataacac tgtgggacgc ttcctcatgt ccctggttaa
     7021 ccaagtccca aagatcgttc cagacgactt cgaaaccatg ctgaactcca acatcaacga
     7081 cctgctcatg gttacttacc tcgccaatct cactcaatcc cagatcgcac tgaacgagaa
     7141 gctggtcaat ctctaaggta cctctagagc ctgcagtctc gacaagcttg tcgagaagta
     7201 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     7261 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     7321 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7381 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7441 ctggatctga tcactgcttg agcctagaag atccggctgc taacaaagcc cgaaaggaag
     7501 ctgagttggc tgctgccacc gctgagcaat aactatcata accatttaaa taaatctata
     7561 tctcaatcgg ggttagtcga taccccaccg agaccccatt gggaccaata cgcccgcgtt
     7621 tcttcctttt ccccacccca acccccaagt tcgggtgaag gcccagggct cgcagccaac
     7681 gtcggggcgg caagccctgc catagccact acgggttaat taaaaactat atctcaatcg
     7741 gggtttaaac aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat
     7801 ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt
     7861 ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg
     7921 cccacaaccg gttgacttgg gtcaactgtc agaccaagtt tactcatata tactttagat
     7981 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8041 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8101 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8161 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8221 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8281 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8341 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8401 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8461 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8521 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8581 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8641 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8701 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8761 catgttcttt cctgcgttat cccctgattg acttgggtcg ctcttcctgt ggatgcgcag
     8821 atgccctgcg taagcgggtg tgggcggaca ataaagtctt aaactgaaca aaatagatct
     8881 aaactatgac aataaagtct taaactagac agaatagttg taaactgaaa tcagtccagt
     8941 tatgctgtga aaaagcatac tggacttttg ttatggctaa agcaaactct tcattttctg
     9001 aagtgcaaat tgcccgtcgt attaaagagg ggcgtggcca agggcatgta aagactatat
     9061 tcgcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat ctcggcttga
     9121 acgaattgtt aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg
     9181 cccaactttg tatagagagc cactgcggga tcgtcaccgt aatctgcttg cacgtagatc
     9241 acataagcac caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg
     9301 ccctgcctcc ggtgctcgcc ggagactgcg agatcataga tatagatctc actacgcggc
     9361 tgctcaaact tgggcagaac gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag
     9421 gcagcaagcg cgatgaatgt cttactacgg agcaagttcc cgaggtaatc ggagtccggc
     9481 tgatgttggg agtaggtggc tacgtctccg aactcacgac cgaaaagatc aagagcagcc
     9541 cgcatggatt tgacttggtc agggccgagc ctacatgtgc gaatgatgcc catacttgag
     9601 ccacctaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctgcgtaac
     9661 atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga
     9721 tgcccgaggc atagactgta c
//
