LOCUS       sequence-494622-       10015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Arl13B (resistance to sgRNA), tagged with GFP, with Y86C 
            mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Mahajan D, Chia HM, Lu L
  TITLE     Knocking out ARL13B completely abolishes primary ciliogenesis in 
            cell lines.
  JOURNAL   Sci Rep. 2025 Jul 24;15(1):26980. doi: 10.1038/s41598-025-11124-5.
  PUBMED    40707593
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2025-07-24"; volume: "15(1):26980. doi"; pages: " 
            10.1038/s41598-025-11124-5"
COMMENT     Sequence Label: ARL13B-(Y86C)-GFP
FEATURES             Location/Qualifiers
     source          1..10015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2200..2503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2504..2707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2658..2678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2704..2728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(4116..4118,4119..4121,4122..4832)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4116..4118,4119..4121,4122..4832)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4161..4182)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4422..4441)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4769..4790
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4849..5348
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4884..4907)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5281..5300
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5369..5968
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5369..5388)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5865..5885
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5982..6570
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6035..6055)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6453..6464)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6777..7410
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7539..7555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7539..7555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7552..7574)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7563..7579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7587..7593,7594..7611,7612..7617))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7632..7653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7770..7787)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7941..8529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8021..8040)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8700..9491,9492..9560))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9323..9342
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9561..9665)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9713..9847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9750..9769)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9804..9823
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10015,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     2221 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2281 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2341 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2401 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2461 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2521 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2581 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2641 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2701 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2761 ccatagaaga caccgactct actagaggat cgctagcgct accggactca gatctcgagg
     2821 ccaccatgtt cagtctcatg gcaagctgtt gtggatggtt taaacgctgg cgggagcctg
     2881 ttagaaaggt gactcttttg atggtgggac ttgataatgc tggtaaaacc gcaacagcaa
     2941 agggaatcca aggagaatac cctgaggacg tggcgccaac cgtcggattt tcaaaaatta
     3001 accttagaca gggaaagttt gaagtcacca tctttgactt gggaggtgga ataagaattc
     3061 ggggaatctg gaagaattac tgtgctgaat cctatggggt aatatttgtt gtggattcca
     3121 gtgatgaaga gagaatggaa gagacaaaag aggctatgtc agaaatgcta agacatcctc
     3181 gtatcagtgg gaaacccata ttggtgttgg caaataaaca agataaagaa ggagcttcag
     3241 gagaagctga tgtcattgaa tgtctatctc tggaaaaatt ggtcaatgag cacaagtgcc
     3301 tgtgtcagat agaaccatgc tccgccattt ccggctatgg aaagaaaatt gacgagtcca
     3361 ttaaaaaagg cctttattgg ctgctacatg ttattgcaag agactttgat gccttaaatg
     3421 aacgcatcca aaaagagaca acagagcagc gtgctcttga ggaacaagag aaacaagaaa
     3481 gagctgaacg agtgcgaaaa ttacgagaag aaagaaaaca aaatgaacag gagcaggctg
     3541 aactcgatgg aaccagtggt ctggctgagt tggacccaga accaacgaat cctttccagc
     3601 caatagcatc tgtaatcatt gagaacgaag gaaaacttga aagagagaaa aaaaaccaaa
     3661 aaatggagaa agacagtgat ggctgccacc tgaaacataa aatggagcat gagcaaatag
     3721 agacacaagg ccaggttaat cacaatggcc aaaaaaataa tgaatttgga ctagtagaaa
     3781 attataagga ggcattaaca cagcagttaa agaatgaaga tgagacagac cggccatcat
     3841 tggaatcagc taatggtaaa aagaaaacta agaaactaag aatgaaaagg aaccaccggg
     3901 tagaaccact taatatagat gactgtgctc ctgagagtcc aacgccaccc ccaccccctc
     3961 ctcctgttgg ctggggaacc cctaaagtca ctagacttcc aaaacttgag cctcttggtg
     4021 aaacacatca taatgatttc tataggaagc cgctgcctcc cctggctgtg ccacagcgac
     4081 ctaacagtga tgctcatgat gtgatctcag gatccatggt gagcaagggc gaggagctgt
     4141 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4201 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     4261 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     4321 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4381 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4441 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4501 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     4561 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     4621 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     4681 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     4741 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     4801 ggatcactct cggcatggac gagctgtaca agtctagata attctaccgg gtaggggagg
     4861 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     4921 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     4981 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     5041 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     5101 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     5161 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     5221 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     5281 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     5341 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     5401 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     5461 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     5521 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     5581 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     5641 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     5701 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     5761 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     5821 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     5881 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     5941 gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca
     6001 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6061 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6121 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6181 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6241 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6301 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6361 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     6421 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6481 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     6541 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga
     6601 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     6661 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     6721 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga
     6781 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     6841 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     6901 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     6961 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     7021 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     7081 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     7141 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     7201 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     7261 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7321 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7381 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7441 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt
     7501 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7561 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7621 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7681 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7741 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7801 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7861 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7921 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7981 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8041 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8101 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8161 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8221 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8281 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8341 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8401 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8461 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8521 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8581 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8641 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8701 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8761 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8821 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8881 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8941 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9001 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9061 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9121 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9181 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9241 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9301 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9361 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9421 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9481 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9541 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9601 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9661 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt
     9721 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9781 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9841 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     9901 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     9961 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagtt
//
