LOCUS       sequence-49464-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  TITLE     Targeting Vector to Generate an LCA allele for Foxd3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Foxd3 LCA TV
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(118..135)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    124..348
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    414..447
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
       61 atcgccttct tgacgagttc ttctgagggg atcaattctc tagagctcgc tgatcagcct
      121 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
      181 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
      241 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
      301 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
      361 aaagaaccag ctggggctcg actagagctt gcggaaccct tgaattcctg cagataactt
      421 cgtataaagt atcctatacg aagttatgga tccaggtacc ttcgaatgat gagctagtgg
      481 ccgggccgga cggcggcctc tggcagcagc ctgctcttcc aggcggtacg ccctctctga
      541 tcctggtcca tctgtcctgg tcctggtcct ggtccctcta cccaatcctg gactctgcta
      601 cccgcccccg actccccaac actgaccaac agcggcctcc atcccctcac tcacctaagc
      661 tggcggagca aactcaaccc gcgtccgctg ggaataactt tccgtacagg taaaactgat
      721 aaccgaattt ccaaaaatgc accccgacgg cgcctg
//
