LOCUS       sequence-494652-        9369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector that can express destabilized uTEV3. Expressed off
            of CMV promoter with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Chacko AN, Dhanabalan KM, Wan J, Chien R, Anderson NT, Xu B, Pham K,
            Tiwari R, Mukherjee A
  TITLE     A programmable genetic platform for engineering noninvasive 
            biosensors
  JOURNAL   bioRxiv 2025.09.07.674790
REFERENCE   2  (bases 1 to 9369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.07.674790"
COMMENT     Sequence Label: pJW53(uTEV3-DHFR2.0-IRES-mCherry)
FEATURES             Location/Qualifiers
     source          1..9369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      518..1106
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1007..1026
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1260..1277
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1394..1415
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1430..1435,1436..1453,1454..1460)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1468..1484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1473..1495
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1492..1508
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1492..1508
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1527..1547
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1529..1547
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1575..1801
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1802..1982
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2029..2154
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2647..2880
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3065..3109
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3376..3493
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3515..3818
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3819..4022
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3973..3993
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    4045..4125
                     /label=MCS
                     /note="multiple cloning site"
     CDS             4155..4178
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4179..4886
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGTFWKHWIQTKDGQCGNPLVSTRDGFI
                     VGIHSASNFTNTNNYFASVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
     CDS             4881..5363
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MNISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTRVKSVDEAIAACGDVPEIMVIGGGRVYEQF
                     LPKAQKLYLSHIDAEVDGDTHFPDYEPDDWESVFSEFHDADALNSHSYCFEILERR"
     misc_feature    join(5364..5938,5939..5941,5942..5950)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5531..5548)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5758..5777
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5951..6661
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6096..6114)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6364..6383
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(6686..6710)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6741..7329
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6794..6814)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7212..7223)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7462..7695
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7767..7888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7804..7823)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7858..7877
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7928..8063
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8083..8102)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8084..8102)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8112..8129)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8112..8128)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8121..8143)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8270..8725
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8357..8376)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8567..8588
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8751..8855
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8856..8924,8925..9369,1..347)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9074..9093)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caaacgatga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
       61 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
      121 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
      181 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
      241 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
      301 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
      361 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
      421 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      481 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      541 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      601 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      661 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      721 catacctcgc tctgcaaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      781 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      841 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      901 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      961 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1021 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1081 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1141 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1201 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaact accgagcgca
     1261 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     1321 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     1381 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     1441 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     1501 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaatctgg
     1561 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     1621 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     1681 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     1741 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     1801 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     1861 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     1921 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     1981 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     2041 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     2101 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     2161 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     2221 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     2281 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     2341 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     2401 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     2461 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     2521 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     2581 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     2641 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     2701 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     2761 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     2821 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     2881 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     2941 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     3001 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     3061 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     3121 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     3181 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     3241 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     3301 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     3361 acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     3421 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     3481 aattcaaaat tttatcgatc acgagactag cctccgttac ataacttacg gtaaatggcc
     3541 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     3601 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     3661 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     3721 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     3781 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     3841 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     3901 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     3961 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     4021 ctggtttagt gaaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct
     4081 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgagc tcggatccag
     4141 tacccttcac catggactac aaggacgacg acgacaaggg agaaagcttg tttaaggggc
     4201 cgcgtgatta caacccgata tcgagcacca tttgtcattt gacgaatgaa tctgatgggc
     4261 acacaacatc gttgtatggt attggatttg gtcccttcat cattacaaac aagcacttgt
     4321 ttagaagaaa taatggaaca ctgttggtcc aatcactaca tggtgtattc aaggtcaaga
     4381 acaccacgac tttgcaacaa cacctcattg atgggaggga catgataatt attcgcatgc
     4441 ctaaggattt cccaccattt cctcaaaagc tgaaatttag agagccacaa agggaagagc
     4501 gcatatgtct tgtgacaacc aacttccaaa ctaagagcat gtctagcatg gtgtcagaca
     4561 ctagttgcac attcccttca tctgatggca cattctggaa gcattggatt caaaccaagg
     4621 atgggcagtg tggcaatcca ttagtatcaa ctagagatgg gttcattgtt ggtatacact
     4681 cagcatcgaa tttcaccaac acaaacaatt atttcgcaag cgtgccgaaa aacttcatgg
     4741 aattgttgac aaatcaggag gcgcagcagt gggttagtgg ttggcgatta aatgctgact
     4801 cagtattgtg ggggggccat aaagttttca tggtgaaacc tgaagagcct tttcagccag
     4861 ttaaggaagc gactcaactc atgaatatca gtctgattgc ggcgttagcg gtagatcgcg
     4921 ttatcggcat ggaaaacgcg atgccgtgga acctgcctgc cgatctcgcc tggtttaaac
     4981 gcaacacctt aaataaaccc gtgattatgg gccgccatac ctgggaatca atcggtcgtc
     5041 cgttgccagg acgcaaaaat attatcctca gcagtcaacc gggcacggac gatcgcgtaa
     5101 cgagagtgaa gtcggtggat gaagccatcg cggcgtgtgg tgacgtacca gaaatcatgg
     5161 ttattggcgg cggtcgcgtt tatgaacagt tcttgccaaa agcgcaaaaa ctgtatctga
     5221 gccatatcga cgcagaagtg gatggcgaca cccatttccc ggattacgag ccggatgact
     5281 gggaatcggt attcagcgaa ttccacgatg ctgatgcgct taactctcac agctattgct
     5341 ttgagattct ggagcggcga tgacccctct ccctcccccc cccctaacgt tactggccga
     5401 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     5461 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     5521 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     5581 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     5641 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     5701 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     5761 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     5821 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     5881 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat
     5941 ggccacaacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     6001 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     6061 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     6121 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     6181 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     6241 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     6301 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     6361 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     6421 cgaggacggc gccctgaagg gcgagatcag gcagaggctg aagctgaagg acggcggcca
     6481 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     6541 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     6601 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     6661 gagcggccgc actcgagata tctagaccca gctttcttgt acaaagtggt tgatatccag
     6721 cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6781 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6841 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6901 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6961 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7021 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7081 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     7141 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7201 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7261 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7321 cctccccgcc tggaattctg cagatatccg gactagtgat ctaattctac cggggatccg
     7381 tcgagggccc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7441 ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc
     7501 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7561 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7621 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7681 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7741 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7801 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7861 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7921 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7981 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8041 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     8101 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     8161 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     8221 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     8281 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8341 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8401 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8461 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8521 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8581 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8641 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     8701 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8761 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8821 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8881 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8941 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9001 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9061 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9121 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9181 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9241 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9301 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9361 aagccatac
//
