LOCUS       sequence-49478-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Mitchell SF, Walker SE, Algire MA, Park EH, Hinnebusch AG, Lorsch JR
  TITLE     The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to 
            stimulate canonical translation initiation and to block an 
            alternative pathway.
  JOURNAL   Mol Cell. 2010 Sep 24;39(6):950-62.
  PUBMED    20864040
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2010-09-24"; volume: "39(6)"; pages: "950-62"
COMMENT     Sequence Label: pET-DUET-GST-eIF4G1-His6, eIF4E
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..52)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    749..773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(773..792)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(774..792)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagtagacga gtccatgtgc tggcgttcaa atttcgcagc agcggtttct ttaccagact
       61 cgagttacaa ggtgattgat ggttgagggt gtctaccatt ggcactggaa tgtggaaaga
      121 attccaaatg cccgtcatcg gttaatttta aaacttgctt gaatttacca ccaattctca
      181 atagtggttc tttgtcttca gatttagtcc ataaggcaaa cttgttacca ccttttctaa
      241 tgcttaaaac gacaccgtta atttgggagt cgtcttcatc aattgtttca ccaataactg
      301 ctagtaaagt tcttagccat aattcatcaa tatcagcacc ttttcctcta agttggaaag
      361 accatttacc acctttagca ttggcttcat cttcccattc aggtctaacg tcattacgga
      421 agacgtggta atctgatttc aatggtagtt cgtgtggctc aggaatattt tgaatgatag
      481 cccaaaattc ttcaacagtt tggaatgaag tgacgggacg taatagatca gaccacgact
      541 cagatttatc gacggctggc tttgtgtacc ataaagtcca tttggtgttc aatgggtgct
      601 tgacatcgaa gtgagcactg tcacttaaaa cagtctttgg agtagctgtg gtatcatcga
      661 ctgaaacgtt ttcttcaaac ttcttgctaa cttcttcaac ggacatatgt atatctcctt
      721 cttatactta actaatatac taagatgggg aattgttatc cgctcacaat tcccctatag
      781 tgagtcgtat taatttcgat tatgcggccg tgtacaatac gattactttc
//
