LOCUS       sequence-49479-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Fang S, Ferrone M, Yang C, Jensen JP, Tiwari S, Weissman AM
  TITLE     The tumor autocrine motility factor receptor, gp78, is a ubiquitin 
            protein ligase implicated in degradation from the endoplasmic 
            reticulum.
  JOURNAL   Proc Natl Acad Sci U S A. 2001 Dec 4. 98(25):14422-7.
  PUBMED    11724934
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2001 Dec 4. 98(25):14422-7."
COMMENT     Sequence Label: pEGFP-N1 gp78fl
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    151..272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(188..207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     242..261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(279..734)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(416..437)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     628..647
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat
       61 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
      121 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
      181 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
      241 tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt
      301 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
      361 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
      421 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
      481 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
      541 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
      601 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
      661 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
      721 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      781 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      841 tcaataatat gaaga
//
