LOCUS       sequence-494794-       14604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to express the GUS gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14604)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 14604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVT-GUS
FEATURES             Location/Qualifiers
     source          1..14604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    938..1111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1147..2955)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="RPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAV
                     PGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVME
                     HQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFF
                     NYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVV
                     ATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLI
                     NHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWAD
                     EHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKN
                     HPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLF
                     DVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTD
                     MWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPK
                     SAAFLLQKRWTGMNFGEKPQQGGKQE"
     CDS             complement(join(1153..2970,3161..3175))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          2971..3160
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(3244..3920)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(3287..3307)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(7690..7707)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7690..7706)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7699..7721)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7909..7933
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9234..9863
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10292..11365
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11431..11625
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11861..11883)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11969..12109
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12024..12043
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12124..12141)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12295..12883)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12375..12394)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12970..13764)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    14189..14213
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    14481..14502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14517..14522,14523..14540,14541..14547)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14555..14571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14560..14582
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14579..14595
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14579..14595
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtgat ctggatttta gtatggattt
      961 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     1021 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     1081 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
     1141 cggcatctac tcttgtttgc ctccctgctg cggtttttca ccgaagttca tgccagtcca
     1201 gcgtttttgc agcagaaaag ccgccgactt cggtttgcgg tcgcgagtga agatcccttt
     1261 cttgttaccg ccaacgcgca atatgccttg cgaggtcgca aaatcggcga aattccatac
     1321 ctgttcaccg acgacggcgc tgacgcgatc aaagacgcgg tgatacatat ccagccatgc
     1381 acactgatac tcttcactcc acatgtcggt gtacattgag tgcagcccgg ctaacgtatc
     1441 cacgccgtat tcggtgatga taatcggctg atgcagtttc tcctgccagg ccagaagttc
     1501 tttttccagt accttctctg ccgtttccaa atcgccgctt tggacatacc atccgtaata
     1561 acggttcagg cacagcacat caaagagatc gctgatggta tcggtgtgag cgtcgcagaa
     1621 cattacattg acgcaggtga tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag
     1681 tggcgcgaaa tattcccgtg caccttgcgg acgggtatcc ggttcgttgg caatactcca
     1741 catcaccacg cttgggtggt ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg
     1801 cgcttgctga gtttccccgt tgactgcctc ttcgctgtac agttctttcg gcttgttgcc
     1861 cgcttcgaaa ccaatgccta aagagaggtt aaagccgaca gcagcagttt catcaatcac
     1921 cacgatgcca tgttcatctg cccagtcgag catctcttca gcgtaagggt aatgcgaggt
     1981 acggtaggag ttggccccaa tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt
     2041 atcgaatcct ttgccacgca agtccgcatc ttcatgacga ccaaagccag taaagtagaa
     2101 cggtttgtgg ttaatcagga actgttcgcc cttcactgcc actgaccgga tgccgacgcg
     2161 aagcgggtag atatcacact ctgtctggct tttggctgtg acgcacagtt catagagata
     2221 accttcaccc ggttgccaga ggtgcggatt caccacttgc aaagtcccgc tagtgccttg
     2281 tccagttgca accacctgtt gatccgcatc acgcagttca acgctgacat caccattggc
     2341 caccacctgc cagtcaacag acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt
     2401 gatatcgtcc acccaggtgt tcggcgtggt gtagagcatt acgctgcgat ggattccggc
     2461 atagttaaag aaatcatgga agtaagactg ctttttcttg ccgttttcgt cggtaatcac
     2521 cattcccggc gggatagtct gccagttcag ttcgttgttc acacaaacgg tgatacgtac
     2581 acttttcccg gcaataacat acggcgtgac atcggcttca aatggcgtat agccgccctg
     2641 atgctccatc acttcctgat tattgaccca cactttgccg taatgagtga ccgcatcgaa
     2701 acgcagcacg atacgctggc ctgcccaacc tttcggtata aagacttcgc gctgatacca
     2761 gacgttgccc gcataattac gaatatctgc atcggcgaac tgatcgttaa aactgcctgg
     2821 cacagcaatt gcccggcttt cttgtaacgc gctttcccac caacgctgat caattccaca
     2881 gttttcgcga tccagactga atgcccacag gccgtcgagt tttttgattt cacgggttgg
     2941 ggtttctaca ggacggacga gtcgtcggtt ctgtaactat catcatcatc atagacacac
     3001 gaaataaagt aatcagatta tcagttaaag ctatgtaata tttacaccat aaccaatcaa
     3061 ttaaaaaata gatcagttta aagaaagatc aaagctcaaa aaaataaaaa gagaaaaggg
     3121 tcctaaccaa gaaaatgaag gagaaaaact agaaatttac cctcagatct accatggatc
     3181 tcattgcccc ccggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg
     3241 atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga
     3301 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
     3361 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
     3421 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
     3481 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
     3541 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
     3601 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
     3661 caccatgttc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     3721 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
     3781 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
     3841 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
     3901 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
     3961 tggagtagac gagagtgtcg tgctccacca tgttggcaag ctgctctagc tcacgttgac
     4021 ggaaactgca gaacgatctc agttaggtca tgggaaagct gatattcatc cctctgagac
     4081 tctatataat taaatgcgtt tgggggatta gctaattgag aatccatata gagaaatatg
     4141 gccgcgcagc gacgccggtt gaagaatatg aacaaggaat aagatgaaca aggaagaaga
     4201 tgaacaagca ataatttaat taattattgt taataagcat aaattatgaa aataattttg
     4261 atggtaatga aagtattata aataatattt aggagaaaat attgttaagg aaatatctgg
     4321 ttatgtttat ttgttaaggt atatgggttt ttatagaaac ccaggagcag ctatggtaag
     4381 aaacccagga ctcaaaaaat gcctctcaac ttctctcata tgttgcggag tctggagtcc
     4441 catttatacc atagaaagca ttgggggtat agcatggtaa tttcacatcg gactccatac
     4501 cgcggccatc tatataatat tactgatggc cgcgcgaagt cccggtatgc cttttcagcc
     4561 gttggatctg gagccgtacc tttttcgcgt tcccattttg cccctggagt cccgagctat
     4621 ttacgacccc ctgccaccga gtccccttcg tggagcgctc ttcgtccaac acgtgtccca
     4681 acttttgcgc atttttgact acctgtcgcg cacttgaatt aaagttgccc ttttattttc
     4741 ttccaattat tttgcggctg agtagtctag tggatccccc ggagctccac ttgggatagg
     4801 ttaagaaaat atgtctggct tgtaaacgaa aagaatcggg gtttctaggc attttttaaa
     4861 tagtgaccca ggactcaaaa actagctctc aacttctctc atatgttgtg gagtctggag
     4921 tcctttatat atgtttacgc tatagaaggc atttggggac tctcccgggc ccaccatagg
     4981 actccatacc gcggccatct atataatatt actgatggcc gcgcggattt tggagccccc
     5041 ttttgtcgtt agcgcgacct ttaattcaaa ttaaaggttg cttttttcta tcgtccgatc
     5101 attttgcgcc tgactagcct agatattagc aacaacttcg cggctaagtt gttgaccgtc
     5161 aagtataaat taatgggctt atggcctaat gggtatagag caaaatggca tgcctgcaga
     5221 ctagtgcttt actctgatcc gcagatattt catcaggaaa gagctttgca agttccttca
     5281 ttgtagcatc aataatttct gagtcactac atgcaatcca ttcttctgct ggggcaaaaa
     5341 ctaactccaa catggattgg tttggattgt aatattcctt acatgttaca gacatgtcag
     5401 cataaacact taaaagcggg cttctgctaa agagtagatg atcataggtg ttcttcaatt
     5461 tcctatcaaa ccagatgtga acgttgataa ctggaactcc aactaatttc tctaatttct
     5521 tgaagtatga aatctctctc cagtcttcag gcactagtga attcgctagc gttaactggc
     5581 cataatcctg tgtatgcaac gttgaaaatt cggatctatt tttacgattc gatcatgaat
     5641 taataaaatt taaattttat tgaatgattt tctagtacat aactgacata cgacttatca
     5701 gttgcgaaac gaacagctct aattacattg ttaagtgaaa ttacgcctaa ttcgtctaag
     5761 tacaacataa ccaactgttt aaacctaatt aaataagtcg acccagaagc tgtcagagat
     5821 gtcgtccaga cttggaagtt caggtaagct ttgtggagat gcaacgctct ccgtaagttg
     5881 tggttgaatc ggatctggac gtggtatata cgcgtcctgg tgtatagcag atcctctact
     5941 ccgtatatct tgaaatatag gggatttatt atctcccaga tatacacgcc attctctgcc
     6001 tgacgtgcag tgatgatttc ccctgtgcgt gaatccatga ccggtgcagc ctatgtggaa
     6061 gtatatggtg cacccacagt ttagatcaat gcgtcgtcgt ctaatcgccc tcctcttggc
     6121 ttgcctgtgt gccttcttga tagagggggg cgtcgagagt gatgaagata acattcttga
     6181 gagtccaatg tttgagcgac gcattttccg ctttatccag gaaatcttta tagctggatc
     6241 cctcgcctgg attgcaaagc acgattgaag ggatccctcc tttaatttga actggtttcc
     6301 cgtacttgca atttgactgc cagtcttttt gggccccaag aagctctttc caatgcttta
     6361 gctttaggta ttgcggtgct acgtcatcaa tgacgttata ggcaacatta ttcgagtaca
     6421 ctcgattatt gaagtccagg tgtccactga ggtaattatg tgggcctaaa gcacgagccc
     6481 acatcgtctt gcccgttcta gaatcacctt ccactatgag actaactggt cttacaggcc
     6541 gcgcagcggg acccattcca aaataatcat ccgcccattc ttgcatctgt tcaggaacgt
     6601 tagtgaaaga ggaaagtgga aatggaggga cccattgttc aggcgccttt ttgaaaaggc
     6661 gttccaagtt cgctgtaatg ttatggtaac tcacgatgaa cgcccttgga tctccggccc
     6721 ggataattgc aagagcttcc gccaaactag gtgcattgat ggcgttataa tagacgtcgt
     6781 ctttattctt ctttgtaccc ccaggcactt tgaactgtcc ggattcacaa caatcgccgt
     6841 ctttggtgat gtaattcttg acggcgttgg cgtctttggc tgcttgtatg tttgggtgaa
     6901 aattggtcga ccttctgggg tgagtaaggt cgaaaaatct cttattcttg atgttggact
     6961 ttccagagag ttggatgaga cagtgaaggt gtgggaatcc gtccgagtgc tcctctctgg
     7021 caactcgaat atatgttggt ttgacgactg accatgataa ggtttgaagc atttgaagag
     7081 cttcattttt tgggatatcg cacttgggat aggttaagaa aatatgtctg gcttgtaaac
     7141 gaaaagaatc ggggtttcta ggcatttttt aaatagtgac ccaggactca aaaactagct
     7201 ctcaacttct ctcatatgtt gtggagtctg gagtccttta tatatgttta cgctatagaa
     7261 ggcatttggg gactctcccg ggcccaccat aggactccat accgcggcca tctatataat
     7321 attactgatg gccgcgcgga ttttggagcc cccttttgtc gttagcgcga cctttaattc
     7381 aaattaaagg ttgctttttt ctatcgtcca atcattttgc gcctgactag cctagatatt
     7441 agcaacaact tcgcgactaa gttgttgacc gtcaagtata aattaatggg cttatggccc
     7501 aatgggtata gagcaaaatg cccaagcgtg atgccccgtg gcgcttatta gcgggtacgt
     7561 ctaaggtaag tcgaaatgcc aactactctc ccttgagagt attgggccct aaagttaata
     7621 agggacctcg agggggggcc cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
     7681 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     7741 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     7801 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag
     7861 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
     7921 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     7981 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     8041 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     8101 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     8161 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     8221 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     8281 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     8341 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     8401 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     8461 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     8521 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     8581 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     8641 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     8701 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     8761 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     8821 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     8881 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     8941 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     9001 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     9061 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     9121 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     9181 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     9241 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     9301 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     9361 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     9421 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     9481 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     9541 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     9601 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     9661 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     9721 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     9781 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     9841 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     9901 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     9961 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
    10021 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
    10081 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
    10141 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
    10201 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg
    10261 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
    10321 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
    10381 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
    10441 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
    10501 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
    10561 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
    10621 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
    10681 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
    10741 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
    10801 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
    10861 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
    10921 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
    10981 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
    11041 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
    11101 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
    11161 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
    11221 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
    11281 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
    11341 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
    11401 aggggaaaaa ggtcgaaaag gtgtctttcc tgtggatagc acgtacattg ggaacccaaa
    11461 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
    11521 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
    11581 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
    11641 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
    11701 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
    11761 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
    11821 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    11881 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    11941 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
    12001 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    12061 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
    12121 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    12181 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    12241 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    12301 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    12361 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    12421 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    12481 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    12541 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    12601 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    12661 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    12721 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    12781 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    12841 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    12901 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca
    12961 ttctaggtac taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg
    13021 cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct
    13081 gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag
    13141 atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc
    13201 gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg
    13261 cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt
    13321 attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc
    13381 cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc
    13441 agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag
    13501 cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc
    13561 ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg
    13621 gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat ataccttagc
    13681 aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc
    13741 cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag
    13801 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
    13861 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
    13921 gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt
    13981 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
    14041 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
    14101 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
    14161 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
    14221 cgcttagaca acttaataac acattgcgga cgtttttaat gtactgaatt aacgccgaat
    14281 taattcgggg atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
    14341 gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa accgcctctc
    14401 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    14461 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    14521 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    14581 ggaaacagct atgacatgat tacg
//
