LOCUS       sequence-494795-       16532 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to express the Ruby gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16532)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 16532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVT-Ruby
FEATURES             Location/Qualifiers
     source          1..16532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    938..1111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(2656..2712)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3547..3603)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        complement(5172..5848)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(5215..5235)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(9618..9635)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9618..9634)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9627..9649)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    9837..9861
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11162..11791
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12220..13293
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13359..13553
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13789..13811)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13897..14037
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13952..13971
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14052..14069)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14223..14811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14303..14322)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14898..15692)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    16117..16141
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    16409..16430
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16445..16450,16451..16468,16469..16475)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16483..16499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16488..16510
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16507..16523
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16507..16523
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtgat ctggatttta gtatggattt
      961 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     1021 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     1081 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
     1141 cggcatctac tctcattgca aagaaggctc aagtttagct tgcataacag catcaagaaa
     1201 atcatcaata gccttaactg aagaaccctt aagttctttc tcttcgttaa cagcatcctt
     1261 caatttcttt cccatctcaa cagccctgtt cttcatctcc catcctttag aaccctcatt
     1321 tctttccaaa atcatctcaa cgatcctctt aactttttcc tttgaaatct ctccttccaa
     1381 acctctagca agctcaacag caacacccat ctcatcttcc aagtacttaa gattgtaagc
     1441 ttgttcagca gccaaaggcc atccaagcat tggaacaccc tccctcaatg attcaagaat
     1501 agagttccat ccgcaatgag taaggaatcc accgatagac tcatggttca agatttcaag
     1561 ttgaggaccc aacttataaa caagttttcc ttgtttctta accttcatcc tctcttcaaa
     1621 accctcagga agccattctg gcctcatctc ttcgttaata tcaaatccga atggagccct
     1681 gataacccac aagaaaggct tctctgaaga ttccaaacca gcagcaagct ccatcatttg
     1741 tgttggtgag atagtgtttt gtgatccgaa agagatgtaa agaacagaat caggctcttt
     1801 caatgaaagc cattgaacaa attcagcacc agtagagtta ttatctgaag atgaatgttg
     1861 aacaggtgaa gcaatcaatg gtccaatacc ccagattgga agctttgtgt agtttctcaa
     1921 gatagagaaa ccaagagttt cgatctcttc aactgaattg caaagccatc caaatgactt
     1981 catagattgc ctcaattgag gttggaagta ttttgaccaa tcatcagaac catcagcata
     2041 tctcaaaaac ctatgaagtt gagatctcct gaatttatgg ttctctggaa atccaggaag
     2101 tggaaattct tgatcatctg agtagttttg atgaggcaaa tcattccaaa ttgaaacgta
     2161 agcagaagta ccataagctc caccagtggt aaaaacaact ccggtagaac caacatcctt
     2221 agcaacctga tcaacccatc caaggaaaac atcatggata acacaaagtg gaggtctagc
     2281 tggtgagaaa tgtctagaga tgtaatccct caaatgagga tcaagtgaga tggtagaatg
     2341 gaaaagtgaa acaaccaatg gaagggtcaa cttatcagtg ttctcaattc ctggaggcaa
     2401 accatggttt gtagaattaa aaggaagttc aacgattcta ataccagatg aagagtatga
     2461 atgatgatgc aaaagatgcc tcaagaatcc agcattaaga ggtgtagtca aaagagtgat
     2521 aataacatga gatctcttat aaaggaacat agcaagttcc aagaatggcc tcaaatgacc
     2581 ttgagccata aaaggaatca taaggatatg ttgtgtctct ccttcaccgt tagtattcat
     2641 cttaatagcg gtcataggac ctgggttctc ctccacatct ccggcctgct taagcaatga
     2701 gaagttggta gctcctgaac cagcagatgt gaacttgtat gatccatggc acaaggtacc
     2761 atgatcccat gaagaatgaa taagctcagc tttccacttt tctccagcag cacccaaaac
     2821 aacatgaagt gggtaaaaat gctcagggaa tggatgagca agcttccagt taggagcctt
     2881 agattcatag atgttaacct cttcgaatct accattgatc aaagctttcc taagccatga
     2941 atcaaaagca gcagcccatg gagcaactcc atcgaaataa tgaggtgtct catcaagtgg
     3001 atgagtagct gatccagaac cgataatcaa aacaccatcg ttcttcaatg gagcaagagc
     3061 cctaccaaga ttgtaatgat atgttccatc caaatgaggt tgaacagaaa gttgacaaac
     3121 aggaatatca gcttctgggt acataagcat caaaggaacc caagctccat gatccaaacc
     3181 tctcttttga tcggtctcag ctgtttcaaa acctgatttc ttcaagatct cttcaacctt
     3241 cctagcaagc tctggttctc caggagctgg gtacttgaat tgatacatag cagcagggta
     3301 atcatcgaaa tcatggatag tatcgttgat atgaacagca ttaacagttg gcttaacggt
     3361 ctcccaatga ccagagataa taagaatagc cttaggtttc tttgagaaga ttttctctct
     3421 ccaagtttca aagaaaggcc tcaatggatg tgtatcttca acagtaagga tagggtttcc
     3481 atgggtgata aagaaagact ccttgatcat ttgatcgtta gcatcttcac cattcatcat
     3541 cttcataggt cctgggttct cttcaacatc tccagcttgt ttcaaaagag aaaaatttgt
     3601 agctcctgaa ccgtatctag ggattgggat aagcttcaat ggcttagtct tttgaagagc
     3661 aataccgaac ttctcatcca tatccaaatc tttaggagag atatctcctt caagcttcca
     3721 gttaaagaat tgcaaaagag tagcaagcat caaggtaagc atcctaatag ccaaattcat
     3781 accagggcag attctccttc cagcaccaaa aggcaaaagt ccgaaatctc tacccttaac
     3841 atcaatctca catccgataa acctttctgg tgagaaaata tcagcgtttt gccaagcatt
     3901 aggatctcta ccaatagccc acaaattaac aaggatttga gcatcttttg gaacaatgta
     3961 tccataaagt tcaacatcag tatcagcctt tctaggcaaa agaaaaacag ttggaggatg
     4021 caacctaagg gtctccttga taatagcttg caaatatgga aggttgataa tatctgattc
     4081 ttggatttgc ttatcctttc caagaacttg cttgatctct tcttgagctt tctccatcat
     4141 ttcaggattc ctaatcaact cggtcataac ccattcaaat gttgaagagg ttgtatcagt
     4201 accagcatcg aagatatcaa ccaaaagatg gttaatctct cccattgtca attcgttttg
     4261 cttgaaaagt tgcaaaagaa catccaaaac atcatcagtg gttgtagtgg ttgtagttga
     4321 agaatctgga gcaagtcttt cacagataat accttggaaa acagcgatca acttatcgaa
     4381 agagcaagca agtctccttc taattcctga tggatcaaca caaccaagga taggaaagta
     4441 atcagcatag tttggctttc cgatatcctc cataatattc cagatcaact ccttgaactc
     4501 ttgtgaggta tgagatttat gatgagcaag ttcaacagaa aagaacaatt ttgacaaaag
     4561 gttaagagag gttgtgaaag cagcctttcc aatatcaaca gcttgacctt tttgagcgca
     4621 ctcttgaacg tattcataaa gttgttgaac cttagcatgt ctaaaggttt gacaagcatc
     4681 caacctttga ggagacaaaa gatgaacagc tgtgatcttt ctgaaatttc tccacttagg
     4741 tgaaactggc aaccaagaca ttgtaagctt atgatgatct ccagcggtaa cagagttagg
     4801 gattgttctg tttgacaatg gatgatcttt cttaaggaac atctctttag caacatcagc
     4861 tgaagaaaca acgatagtgg taactgaacc caatctaaga gaaatcaatg gtccatggat
     4921 cttagcaagg ttagcgaatg acctatgagg tttctttcca acttccaaga tgttaccgat
     4981 aataggaagt ggcttaggtc ctggaggcaa aagctttgta gtttgttgag aaaacaaaag
     5041 cttgataaaa tggaatgaga tgaaccagat agccaaaatc atagcaagag tagcatgatc
     5101 catggatctc attgcccccc ggatctgcga aagctcgaga gagatagatt tgtagagaga
     5161 gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg
     5221 tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa
     5281 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
     5341 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta
     5401 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
     5461 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
     5521 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
     5581 tcgtgctcca ccatgttcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
     5641 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
     5701 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
     5761 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
     5821 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
     5881 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct gctctagctc
     5941 acgttgacgg aaactgcaga acgatctcag ttaggtcatg ggaaagctga tattcatccc
     6001 tctgagactc tatataatta aatgcgtttg ggggattagc taattgagaa tccatataga
     6061 gaaatatggc cgcgcagcga cgccggttga agaatatgaa caaggaataa gatgaacaag
     6121 gaagaagatg aacaagcaat aatttaatta attattgtta ataagcataa attatgaaaa
     6181 taattttgat ggtaatgaaa gtattataaa taatatttag gagaaaatat tgttaaggaa
     6241 atatctggtt atgtttattt gttaaggtat atgggttttt atagaaaccc aggagcagct
     6301 atggtaagaa acccaggact caaaaaatgc ctctcaactt ctctcatatg ttgcggagtc
     6361 tggagtccca tttataccat agaaagcatt gggggtatag catggtaatt tcacatcgga
     6421 ctccataccg cggccatcta tataatatta ctgatggccg cgcgaagtcc cggtatgcct
     6481 tttcagccgt tggatctgga gccgtacctt tttcgcgttc ccattttgcc cctggagtcc
     6541 cgagctattt acgaccccct gccaccgagt ccccttcgtg gagcgctctt cgtccaacac
     6601 gtgtcccaac ttttgcgcat ttttgactac ctgtcgcgca cttgaattaa agttgccctt
     6661 ttattttctt ccaattattt tgcggctgag tagtctagtg gatcccccgg agctccactt
     6721 gggataggtt aagaaaatat gtctggcttg taaacgaaaa gaatcggggt ttctaggcat
     6781 tttttaaata gtgacccagg actcaaaaac tagctctcaa cttctctcat atgttgtgga
     6841 gtctggagtc ctttatatat gtttacgcta tagaaggcat ttggggactc tcccgggccc
     6901 accataggac tccataccgc ggccatctat ataatattac tgatggccgc gcggattttg
     6961 gagccccctt ttgtcgttag cgcgaccttt aattcaaatt aaaggttgct tttttctatc
     7021 gtccgatcat tttgcgcctg actagcctag atattagcaa caacttcgcg gctaagttgt
     7081 tgaccgtcaa gtataaatta atgggcttat ggcctaatgg gtatagagca aaatggcatg
     7141 cctgcagact agtgctttac tctgatccgc agatatttca tcaggaaaga gctttgcaag
     7201 ttccttcatt gtagcatcaa taatttctga gtcactacat gcaatccatt cttctgctgg
     7261 ggcaaaaact aactccaaca tggattggtt tggattgtaa tattccttac atgttacaga
     7321 catgtcagca taaacactta aaagcgggct tctgctaaag agtagatgat cataggtgtt
     7381 cttcaatttc ctatcaaacc agatgtgaac gttgataact ggaactccaa ctaatttctc
     7441 taatttcttg aagtatgaaa tctctctcca gtcttcaggc actagtgaat tcgctagcgt
     7501 taactggcca taatcctgtg tatgcaacgt tgaaaattcg gatctatttt tacgattcga
     7561 tcatgaatta ataaaattta aattttattg aatgattttc tagtacataa ctgacatacg
     7621 acttatcagt tgcgaaacga acagctctaa ttacattgtt aagtgaaatt acgcctaatt
     7681 cgtctaagta caacataacc aactgtttaa acctaattaa ataagtcgac ccagaagctg
     7741 tcagagatgt cgtccagact tggaagttca ggtaagcttt gtggagatgc aacgctctcc
     7801 gtaagttgtg gttgaatcgg atctggacgt ggtatatacg cgtcctggtg tatagcagat
     7861 cctctactcc gtatatcttg aaatataggg gatttattat ctcccagata tacacgccat
     7921 tctctgcctg acgtgcagtg atgatttccc ctgtgcgtga atccatgacc ggtgcagcct
     7981 atgtggaagt atatggtgca cccacagttt agatcaatgc gtcgtcgtct aatcgccctc
     8041 ctcttggctt gcctgtgtgc cttcttgata gaggggggcg tcgagagtga tgaagataac
     8101 attcttgaga gtccaatgtt tgagcgacgc attttccgct ttatccagga aatctttata
     8161 gctggatccc tcgcctggat tgcaaagcac gattgaaggg atccctcctt taatttgaac
     8221 tggtttcccg tacttgcaat ttgactgcca gtctttttgg gccccaagaa gctctttcca
     8281 atgctttagc tttaggtatt gcggtgctac gtcatcaatg acgttatagg caacattatt
     8341 cgagtacact cgattattga agtccaggtg tccactgagg taattatgtg ggcctaaagc
     8401 acgagcccac atcgtcttgc ccgttctaga atcaccttcc actatgagac taactggtct
     8461 tacaggccgc gcagcgggac ccattccaaa ataatcatcc gcccattctt gcatctgttc
     8521 aggaacgtta gtgaaagagg aaagtggaaa tggagggacc cattgttcag gcgccttttt
     8581 gaaaaggcgt tccaagttcg ctgtaatgtt atggtaactc acgatgaacg cccttggatc
     8641 tccggcccgg ataattgcaa gagcttccgc caaactaggt gcattgatgg cgttataata
     8701 gacgtcgtct ttattcttct ttgtaccccc aggcactttg aactgtccgg attcacaaca
     8761 atcgccgtct ttggtgatgt aattcttgac ggcgttggcg tctttggctg cttgtatgtt
     8821 tgggtgaaaa ttggtcgacc ttctggggtg agtaaggtcg aaaaatctct tattcttgat
     8881 gttggacttt ccagagagtt ggatgagaca gtgaaggtgt gggaatccgt ccgagtgctc
     8941 ctctctggca actcgaatat atgttggttt gacgactgac catgataagg tttgaagcat
     9001 ttgaagagct tcattttttg ggatatcgca cttgggatag gttaagaaaa tatgtctggc
     9061 ttgtaaacga aaagaatcgg ggtttctagg cattttttaa atagtgaccc aggactcaaa
     9121 aactagctct caacttctct catatgttgt ggagtctgga gtcctttata tatgtttacg
     9181 ctatagaagg catttgggga ctctcccggg cccaccatag gactccatac cgcggccatc
     9241 tatataatat tactgatggc cgcgcggatt ttggagcccc cttttgtcgt tagcgcgacc
     9301 tttaattcaa attaaaggtt gcttttttct atcgtccaat cattttgcgc ctgactagcc
     9361 tagatattag caacaacttc gcgactaagt tgttgaccgt caagtataaa ttaatgggct
     9421 tatggcccaa tgggtataga gcaaaatgcc caagcgtgat gccccgtggc gcttattagc
     9481 gggtacgtct aaggtaagtc gaaatgccaa ctactctccc ttgagagtat tgggccctaa
     9541 agttaataag ggacctcgag ggggggcccg gtacccgggg atcctctaga gtcgacctgc
     9601 aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     9661 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     9721 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgctagagc
     9781 agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
     9841 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     9901 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
     9961 tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc
    10021 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga
    10081 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag
    10141 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc
    10201 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa
    10261 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac
    10321 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc
    10381 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
    10441 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
    10501 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
    10561 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
    10621 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
    10681 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
    10741 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
    10801 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
    10861 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
    10921 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
    10981 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
    11041 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
    11101 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
    11161 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
    11221 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
    11281 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
    11341 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
    11401 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
    11461 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
    11521 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
    11581 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
    11641 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
    11701 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
    11761 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
    11821 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
    11881 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
    11941 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
    12001 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
    12061 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
    12121 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg cctgccggcc
    12181 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc
    12241 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg
    12301 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
    12361 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt
    12421 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
    12481 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
    12541 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
    12601 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
    12661 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
    12721 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
    12781 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
    12841 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
    12901 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
    12961 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
    13021 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
    13081 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
    13141 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
    13201 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
    13261 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
    13321 gccctagcag gggaaaaagg tcgaaaaggt gtctttcctg tggatagcac gtacattggg
    13381 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
    13441 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
    13501 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
    13561 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
    13621 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
    13681 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
    13741 ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    13801 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    13861 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
    13921 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
    13981 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
    14041 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    14101 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    14161 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    14221 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    14281 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    14341 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    14401 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    14461 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    14521 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    14581 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    14641 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    14701 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    14761 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    14821 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    14881 gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc
    14941 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
    15001 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
    15061 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
    15121 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
    15181 agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc
    15241 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
    15301 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
    15361 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
    15421 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
    15481 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
    15541 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
    15601 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt
    15661 ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct tttctacagt
    15721 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg
    15781 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt
    15841 ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct
    15901 gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag
    15961 cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac
    16021 ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt
    16081 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa
    16141 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa
    16201 cgccgaatta attcggggat agccctttgg tcttctgaga ctgtatcttt gatattcttg
    16261 gagtagacga gagtgtcgtg ctccaccatg ttggcaagct gctctagcca atacgcaaac
    16321 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    16381 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    16441 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    16501 ttcacacagg aaacagctat gacatgatta cg
//
