LOCUS       sequence-49480-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Mitchell SF, Walker SE, Algire MA, Park EH, Hinnebusch AG, Lorsch JR
  TITLE     The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to 
            stimulate canonical translation initiation and to block an 
            alternative pathway.
  JOURNAL   Mol Cell. 2010 Sep 24;39(6):950-62.
  PUBMED    20864040
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2010-09-24"; volume: "39(6)"; pages: "950-62"
COMMENT     Sequence Label: pTYB2-HCR1
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 agaataattt tgtttaactt taagaaggag atatacatat gtcttgggac gacgaagcta
       61 tcaatggttc tatgggtaat gatgacgccg ttttgatgga ctcatgggat gctgaaattg
      121 gtgatgacga acctgtcatg caatcatggg atgcagaaga ggaagaaaag aagcctgccc
      181 caaagccaaa aaaagaacaa ccaaaaaagg ttaaaaaagg gaaagaatcc tccgctgaca
      241 gagccctatt ggatattgac acattggatg aaaagacacg taaagaatta atcaaaaagg
      301 ctgaaatgga gtctgactta aacaatgctg cagatttgtt tgcgggttta ggtgttgccg
      361 aagaacatcc aagggctcgt gctttacaaa aggaacaaga ggaacaagct ctaaaaagac
      421 ccgcctttac caaggacaca cctatcgaaa cacatccttt gtttaacgcc gaaacaaaga
      481 gagagtacca ggatttaaga aaggctctta ctgctgctat tacaccaatg aacaaaaaat
      541 ctccgctgaa ctactcctca tcgttagcga ttgatttgat tagagatgta gcaaaaccaa
      601 tgtccatcga atctataaga caaacagttg ctactttgaa cgttttgatc aaagataagg
      661 aaagagagga gaggcaagct cgtctggccc gtgttagagg tggtactgct actgggggtg
      721 ctggtaagaa gaaagtcaaa ggtaagacca atttgggtgg cgcatttaag aaagatcaag
      781 atttcgacct agatggcccc gacgattttg aattcggtga tgacgacttt
//
