LOCUS       sequence-49484-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Joyce PI, Satija R, Chen M, Kuwabara PE
  TITLE     The atypical calpains: evolutionary analyses and roles in 
            Caenorhabditis elegans cellular degeneration.
  JOURNAL   PLoS Genet. 2012 Mar;8(3):e1002602. Epub 2012 Mar 29.
  PUBMED    22479198
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2012-03"; volume: "8(3)"; pages: "e1002602. Epub 
            2012 Mar 29"
COMMENT     Sequence Label: pPJ-1
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             249..269
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             291..311
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             333..353
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             375..395
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(538..556)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa
       61 atcactcaca acgatggata cgctaacaac ttggaaatga aataagcttg catgcctgca
      121 ggtcgactct agaggatccc cgggattggc caaaggaccc aaaggtatgt ttcgaatgat
      181 actaacataa catagaacat tttcaggagg acccttggag ggtaccgagc tcagaaaaaa
      241 tgactgctcc aaagaagaag cgtaaggtac cggcagaaaa aatgactgct ccaaagaaga
      301 agcgtaaggt accggcagaa aaaatgactg ctccaaagaa gaagcgtaag gtaccggcag
      361 aaaaaatgac tgctccaaag aagaagcgta aggtaccggt agaaaaaatg gcctcctccg
      421 aggacgtcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc
      481 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      541 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      601 tccagtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
      661 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      721 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
      781 gcggcacc
//
