LOCUS       sequence-494845-       10029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression construct for N-terminal His-GFP-tagged RAD54L2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Zhang H, Xiong Y, Sun Y, Park JM, Su D, Feng X, Keast S, Tang M, 
            Huang M, Wang C, Srivastava M, Yang C, Zhu D, Chen Z, Li S, Yin L, 
            Pommier Y, Chen J
  TITLE     RAD54L2-mediated DNA damage avoidance pathway specifically preserves
            genome integrity in response to topoisomerase 2 poisons.
  JOURNAL   Sci Adv. 2023 Dec 8;9(49):eadi6681. doi: 10.1126/sciadv.adi6681. 
            Epub 2023 Dec 6.
  PUBMED    38055811
REFERENCE   2  (bases 1 to 10029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-12-8"; volume: "9(49):eadi6681. doi"; pages: " 
            10.1126/sciadv.adi6681. Epub 2023 Dec 6"
COMMENT     Sequence Label: pFastBac1-His-GFP-RAD54L2
FEATURES             Location/Qualifiers
     source          1..10029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             191..208
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(218..220,221..931)
                     /codon_start=1
                     /product="enhanced GFP (EGFP) derivative that exhibits 
                     robust folding and enhanced photostability (Zhong et al., 
                     2019)"
                     /label=SiriusGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(260..281)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     868..889
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             941..961
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    965..989
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5397..5421)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5429..5470
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    5616..5750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5653..5672)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5707..5726
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5779..5944
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      6128..6582
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6215..6234)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6424..6445
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6608..6712
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6713..6781,6782..7573)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6931..6950)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7744..8332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8233..8252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8486..8503
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  8637..8861
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(8928..9461)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(9650..9655,9656..9672,9673..9678))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atcccggtcc gaagcgcgcg gaattcaaag gcctacgtcg acgagctcac
      181 tagtcgcatg caccatcacc atcaccatgg ttcttctgtg agcaagggcg aggagctgtt
      241 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      301 cgtgcgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga agttcatctg
      361 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      421 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      481 gcccgaaggc tacgtccagg agcgcaccat ctccttcaag gacgacggca cctacaagac
      541 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      601 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact tcaacagcca
      661 caacgtctat atcacggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg
      721 ccacaacgtc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      781 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      841 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      901 gatcactctc ggcatggacg agctgtacaa ggggatcgag gaaaacctgt acttccaatc
      961 ctcaacaagt ttgtacaaaa aagcaggctt caccatgtca gacgaatctg cctcagggag
     1021 cgatccagac ctggacccgg acgtggagct ggaggatgcg gaagaggagg aggaggagga
     1081 ggaggtggca gtggaggagt gtgacaggga tgatgaagaa gacctgctgg atgacccatc
     1141 cctggaaggc atgtgtggca ctgagcatgc ccagttggga gaagatgggc agcagccgcc
     1201 gcggtgcact tcaactacct catctcagtc tgagccttca gagcagctta ggcgccacca
     1261 aggcaagaac ctagcctccg aggaccccaa aaagaagaga gctcagaagc cctcccacat
     1321 gagaagaaac atacgaaagc tactccggga ggatcaattg gagcctgtta ccaaagcagc
     1381 acagcaagaa gagttggaaa gaaggaagcg cctggagcag cagaggaaag attatgcagc
     1441 ccctattcct actgttccgc tggagttcct ccctgaggaa attgctttaa gggcaagtga
     1501 cggtccccaa ctgcctcctc gggtcttggc ccaggaagtc atttgtttgg acagtagcag
     1561 tggcagtgag gatgaaaaaa gcagtcgaga tgaggtgatt gaactgagct ctggagagga
     1621 ggacactctg cacattgtgg acagcagtga atctgtcagt gaagatgatg aggaagaaga
     1681 gaagggtggc acccatgtca atgatgtctt aaaccagcgt gacgcccttg ggcgggtcct
     1741 tgtcaaccta aaccaccctc cagaggagga aaatgtcttc cttgccccac agttggcacg
     1801 ggctgtgaaa cctcatcaga ttggcgggat ccggttcctt tacgataacc tagtggagtc
     1861 tctggagagg tttaagacca gcagtggctt tggctgtatt ctggcccaca gcatgggtct
     1921 ggggaaaact ttgcaagtga tctctttcat cgacgtcctc ttccgccaca cgccagccaa
     1981 aacagtcctt gccattgtgc cggttaatac tcttcagaat tggctggcag agttcaacat
     2041 gtggcttcca cctcctgaag ccctcccggc tgacaacaag cctgaagaag tccagcctcg
     2101 gttctttaaa gttcacatct tgaatgatga gcacaagacg atggcatctc gtgctaaagt
     2161 gatggctgat tgggtgtcag agggtggcgt gctgctgatg gggtacgaga tgtacagact
     2221 cctcactctg aagaaatcat ttgccacagg tagaccgaag aaaaccaaga agcgttctca
     2281 cccagtcatc attgatctag atgaggaaga tcggcagcag gagtttcgga gagagtttga
     2341 gaaggcttta tgccgccctg gccctgatgt agtaatctgt gatgagggac accgcatcaa
     2401 aaactgccag gccagcacct cacaggctct gaagaatatc cgctctcgcc gccgggtggt
     2461 gctgactggg taccctctgc aaaacaacct cattgagtac tggtgcatgg tggactttgt
     2521 gcgcccagac ttccttggca cccggcagga gttcagcaac atgtttgaac gccctatcct
     2581 gaatgggcaa tgtattgaca gcacacctca ggacgtccgc ctcatgcggt accggagcca
     2641 tgtcctgcac agtcttctgg agggctttgt gcagaggaga ggccacactg tgctgaagat
     2701 tcatctccct gccaaggaag aaaatgtgat ccttgtgcgg ctctccaaga tccagcgaga
     2761 tttgtataca cagttcatgg atcgcttccg ggactgtggt agcagcggtt ggctggggct
     2821 gaaccccctt aaggcattct gtgtgtgttg caagatctgg aatcaccctg atgtgctgta
     2881 tgaagccctt cagaaggaga gcttggccaa tgagcaggac ctagacgtgg aagaacttgg
     2941 ctctgcaggg accagtgccc gctgtccacc acagggaaca aaaggcaagg gagaggatag
     3001 caccttggct tcctcgatgg gagaggcaac caatagcaag ttcctacagg gcgttggctt
     3061 caaccctttc caggagcgag gcaacaacat tgtcacatat gaatgggcca aggaccttct
     3121 gactaattac cagactggag tcctagaaaa ctctcccaag atggtactgc ttttccacct
     3181 gattgaggaa agtgtgaagc ttggggacaa gatccttgtg tttagccaga gtctttccac
     3241 cttggctctc atcgaggaat tccttggaaa acgagaagta ccctgtccac ctggtaccga
     3301 ggggcaagga gcacagaagt gggttcgaaa catcagctac ttccggctag atggtagcac
     3361 ccctgccttt gagagggagc ggcttattaa tcagttcaat gatcccagca acctcaccac
     3421 ctggctgttc cttctctcta caagggccgg atgcttgggt gtgaatctga ttggtgccaa
     3481 ccgagtggtg gtgtttgatg cttcctggaa cccttgccat gatgcccagg cagtatgtcg
     3541 ggtataccgt tatggccaga aaaagccctg ttacatctat cgccttgtgg ctgattacac
     3601 tctagaaaag aagatctatg accgtcagat ttccaagcag ggcatgtcag atcgggtggt
     3661 ggatgatcta aatccaatgc tgaacttcac acggaaagag gtggaaaacc tactgcactt
     3721 tgttgagaag gagccagctc cccaagtttc cttgaacgta aaggggatca aggagtcagt
     3781 cctgcaactg gcctgtctga agtaccctca cctcatcacc aaggagcctt tcgagcatga
     3841 gtcattgctc ttgaaccgaa aggatcacaa gctaaccaag gctgagaaaa aagcagcaaa
     3901 gaaaagctat gaggaagaca aacgcacatc agtcccctat acccgcccat cgtatgcgca
     3961 gtattaccct gccagcgatc agagcctgac cagcatcccc gccttcagcc agagaaactg
     4021 gcagccaact ttgaagggtg atgaaaagcc tgtggccagt gttcgtcctg tgcagtccac
     4081 ccccatcccc atgatgcccc ggcatgtccc attgggagga agtgtaagct ctgcctccag
     4141 cacaaatcca tccatgaact ttcccatcaa ctacttgcag cgtgcaggag tccttgtgca
     4201 gaaggtggtc accacgacag atattgttat tcctggactc aacagctcca cagatgtaca
     4261 ggcaagaatt aatgctggtg agagcatcca catcatccgt gggacaaaag ggacgtacat
     4321 ccgtaccagt gatggacgga tctttgctgt ccgggcaact ggcaaaccaa aggttcctga
     4381 agatggtcgg atggctgcct caggttccca gggaccttct tgcgagtcca caagcaacgg
     4441 cagacacagt gcctcatcac ccaaagcccc cgaccctgag gggctggcca ggcccgtctc
     4501 tcctgacagc ccagagatca tcagtgagct tcagcagtat gcagatgtgg ctgctgcccg
     4561 ggaatcccgt cagagctccc caagcaccaa tgccgccctg cctggccccc cggcccaact
     4621 tatggacagc agtgctgttc ccgggacagc tctcggaact gagcctcgac tagggggtca
     4681 ttgcctcaat agttccctct tggtgactgg ccagccctgt ggtgacaggc acccagtgct
     4741 ggacttaagg ggccacaagc gaaagttggc cacaccacct gctgcccagg agtcatcccg
     4801 ccggcggtcc aggaagggtc atctgccagc ccccgtgcag ccgtatgaac acgggtatcc
     4861 agtctctggc gggtttgcca tgccacccgt ctccttaaac cataacctca ccaccccctt
     4921 cacctcccag gctggggaga actccctgtt tatgggcagt accccctcct actaccagct
     4981 gtccaatttg ctggcagatg cccgcctggt gtttccagtg actactgacc ctctggtgcc
     5041 agcaggcccc gtcagttcct cttccacggc tacctcagtc actgccagca acccctcctt
     5101 catgctcaac ccttctgtgc cagggatact acccagctat tcactcccat tctcacagcc
     5161 actcctgtcc gagccgagga tgtttgcgcc ttttccttcc cctgtcttgc ccagcaacct
     5221 ttcgcggggc atgtctatct atccaggcta catgtcccca catgcaggct acccagctgg
     5281 tggcctccta cggtcccagg tgcctccatt tgactctcat gaggttgccg aggttgggtt
     5341 cagctccaat gatgatgagg ataaagacga tgatgtgata gaggtcactg ggaaacaccc
     5401 agctttcttg tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct
     5461 cgattctacg tagggcatgc ggtaccaagc ttgtcgagaa gtactagagg atcataatca
     5521 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     5581 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     5641 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5701 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc
     5761 ttgagcctag gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt
     5821 ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc
     5881 ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc
     5941 cacagcgggg catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt
     6001 gacaaaccgt catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc
     6061 tcttcgttat taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc
     6121 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6181 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6241 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6301 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6361 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttctta
     6421 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6481 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6541 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     6601 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6661 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6721 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6781 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6841 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6901 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6961 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7021 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7081 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7141 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7201 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     7261 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7321 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7381 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7441 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7501 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7561 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7621 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7681 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7741 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7801 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7861 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7921 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7981 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8041 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8101 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8161 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8221 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8281 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8341 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8401 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8461 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     8521 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag accagccgcg
     8581 taacctggca aaatcggtta cggttgagta ataaatggat gccctgcgta agcgggtgtg
     8641 ggcggacaat aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta
     8701 aactagacag aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg
     8761 gacttttgtt atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat
     8821 taaagagggg cgtggccaag ggcatggtaa agactatatt cgcggcgttg tgacaattta
     8881 ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta
     8941 cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc
     9001 actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg
     9061 gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg
     9121 gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt gggcagaacg
     9181 taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc
     9241 ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct
     9301 acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca
     9361 gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg
     9421 cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tccataacat
     9481 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
     9541 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     9601 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
     9661 cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     9721 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     9781 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     9841 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     9901 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     9961 tcgtttgttc gcccaggact ctagctatag ttctagtggt tggctacgta tactccggaa
    10021 tattaatag
//
