LOCUS       sequence-494857-       13048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct for C-terminal S 
            protein-Flag-Streptavidin binding peptide (SFB)-tagged RAD54L2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13048)
  AUTHORS   Zhang H, Xiong Y, Sun Y, Park JM, Su D, Feng X, Keast S, Tang M, 
            Huang M, Wang C, Srivastava M, Yang C, Zhu D, Chen Z, Li S, Yin L, 
            Pommier Y, Chen J
  TITLE     RAD54L2-mediated DNA damage avoidance pathway specifically preserves
            genome integrity in response to topoisomerase 2 poisons.
  JOURNAL   Sci Adv. 2023 Dec 8;9(49):eadi6681. doi: 10.1126/sciadv.adi6681. 
            Epub 2023 Dec 6.
  PUBMED    38055811
REFERENCE   2  (bases 1 to 13048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-12-8"; volume: "9(49):eadi6681. doi"; pages: " 
            10.1126/sciadv.adi6681. Epub 2023 Dec 6"
COMMENT     Sequence Label: pLEX307-RAD54L2-FL-SFB
FEATURES             Location/Qualifiers
     source          1..13048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5726..5750)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5788..5832
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             5866..5889
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5893..5916
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5926..6039
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     misc_feature    6081..6669
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6134..6154)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6552..6563)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6741..6974
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7052..7173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7089..7108)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7143..7162
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7213..7348
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7368..7387)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7369..7387)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7397..7414)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7397..7413)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7406..7428)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7555..8010
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7642..7661)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7852..7873
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8036..8140
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8141..8209,8210..9001)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8359..8378)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9172..9760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9661..9680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9914..9931
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10048..10069
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10084..10089,10090..10107,10108..10114)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10122..10138
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10127..10149
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10146..10162
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10146..10162
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10181..10201
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10183..10201
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10229..10455
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10456..10636
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10683..10808
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11301..11534
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11719..11763
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11994..12204
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        12016..12212
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12063..12198
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             12244..12846
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(12244..12263)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12740..12760
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12889..13006
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggcttc
     1321 accatgtcag acgaatctgc ctcagggagc gatccagacc tggacccgga cgtggagctg
     1381 gaggatgcgg aagaggagga ggaggaggag gaggtggcag tggaggagtg tgacagggat
     1441 gatgaagaag acctgctgga tgacccatcc ctggaaggca tgtgtggcac tgagcatgcc
     1501 cagttgggag aagatgggca gcagccgccg cggtgcactt caactacctc atctcagtct
     1561 gagccttcag agcagcttag gcgccaccaa ggcaagaacc tagcctccga ggaccccaaa
     1621 aagaagagag ctcagaagcc ctcccacatg agaagaaaca tacgaaagct actccgggag
     1681 gatcaattgg agcctgttac caaagcagca cagcaagaag agttggaaag aaggaagcgc
     1741 ctggagcagc agaggaaaga ttatgcagcc cctattccta ctgttccgct ggagttcctc
     1801 cctgaggaaa ttgctttaag ggcaagtgac ggtccccaac tgcctcctcg ggtcttggcc
     1861 caggaagtca tttgtttgga cagtagcagt ggcagtgagg atgaaaaaag cagtcgagat
     1921 gaggtgattg aactgagctc tggagaggag gacactctgc acattgtgga cagcagtgaa
     1981 tctgtcagtg aagatgatga ggaagaagag aagggtggca cccatgtcaa tgatgtctta
     2041 aaccagcgtg acgcccttgg gcgggtcctt gtcaacctaa accaccctcc agaggaggaa
     2101 aatgtcttcc ttgccccaca gttggcacgg gctgtgaaac ctcatcagat tggcgggatc
     2161 cggttccttt acgataacct agtggagtct ctggagaggt ttaagaccag cagtggcttt
     2221 ggctgtattc tggcccacag catgggtctg gggaaaactt tgcaagtgat ctctttcatc
     2281 gacgtcctct tccgccacac gccagccaaa acagtccttg ccattgtgcc ggttaatact
     2341 cttcagaatt ggctggcaga gttcaacatg tggcttccac ctcctgaagc cctcccggct
     2401 gacaacaagc ctgaagaagt ccagcctcgg ttctttaaag ttcacatctt gaatgatgag
     2461 cacaagacga tggcatctcg tgctaaagtg atggctgatt gggtgtcaga gggtggcgtg
     2521 ctgctgatgg ggtacgagat gtacagactc ctcactctga agaaatcatt tgccacaggt
     2581 agaccgaaga aaaccaagaa gcgttctcac ccagtcatca ttgatctaga tgaggaagat
     2641 cggcagcagg agtttcggag agagtttgag aaggctttat gccgccctgg ccctgatgta
     2701 gtaatctgtg atgagggaca ccgcatcaaa aactgccagg ccagcacctc acaggctctg
     2761 aagaatatcc gctctcgccg ccgggtggtg ctgactgggt accctctgca aaacaacctc
     2821 attgagtact ggtgcatggt ggactttgtg cgcccagact tccttggcac ccggcaggag
     2881 ttcagcaaca tgtttgaacg ccctatcctg aatgggcaat gtattgacag cacacctcag
     2941 gacgtccgcc tcatgcggta ccggagccat gtcctgcaca gtcttctgga gggctttgtg
     3001 cagaggagag gccacactgt gctgaagatt catctccctg ccaaggaaga aaatgtgatc
     3061 cttgtgcggc tctccaagat ccagcgagat ttgtatacac agttcatgga tcgcttccgg
     3121 gactgtggta gcagcggttg gctggggctg aaccccctta aggcattctg tgtgtgttgc
     3181 aagatctgga atcaccctga tgtgctgtat gaagcccttc agaaggagag cttggccaat
     3241 gagcaggacc tagacgtgga agaacttggc tctgcaggga ccagtgcccg ctgtccacca
     3301 cagggaacaa aaggcaaggg agaggatagc accttggctt cctcgatggg agaggcaacc
     3361 aatagcaagt tcctacaggg cgttggcttc aaccctttcc aggagcgagg caacaacatt
     3421 gtcacatatg aatgggccaa ggaccttctg actaattacc agactggagt cctagaaaac
     3481 tctcccaaga tggtactgct tttccacctg attgaggaaa gtgtgaagct tggggacaag
     3541 atccttgtgt ttagccagag tctttccacc ttggctctca tcgaggaatt ccttggaaaa
     3601 cgagaagtac cctgtccacc tggtaccgag gggcaaggag cacagaagtg ggttcgaaac
     3661 atcagctact tccggctaga tggtagcacc cctgcctttg agagggagcg gcttattaat
     3721 cagttcaatg atcccagcaa cctcaccacc tggctgttcc ttctctctac aagggccgga
     3781 tgcttgggtg tgaatctgat tggtgccaac cgagtggtgg tgtttgatgc ttcctggaac
     3841 ccttgccatg atgcccaggc agtatgtcgg gtataccgtt atggccagaa aaagccctgt
     3901 tacatctatc gccttgtggc tgattacact ctagaaaaga agatctatga ccgtcagatt
     3961 tccaagcagg gcatgtcaga tcgggtggtg gatgatctaa atccaatgct gaacttcaca
     4021 cggaaagagg tggaaaacct actgcacttt gttgagaagg agccagctcc ccaagtttcc
     4081 ttgaacgtaa aggggatcaa ggagtcagtc ctgcaactgg cctgtctgaa gtaccctcac
     4141 ctcatcacca aggagccttt cgagcatgag tcattgctct tgaaccgaaa ggatcacaag
     4201 ctaaccaagg ctgagaaaaa agcagcaaag aaaagctatg aggaagacaa acgcacatca
     4261 gtcccctata cccgcccatc gtatgcgcag tattaccctg ccagcgatca gagcctgacc
     4321 agcatccccg ccttcagcca gagaaactgg cagccaactt tgaagggtga tgaaaagcct
     4381 gtggccagtg ttcgtcctgt gcagtccacc cccatcccca tgatgccccg gcatgtccca
     4441 ttgggaggaa gtgtaagctc tgcctccagc acaaatccat ccatgaactt tcccatcaac
     4501 tacttgcagc gtgcaggagt ccttgtgcag aaggtggtca ccacgacaga tattgttatt
     4561 cctggactca acagctccac agatgtacag gcaagaatta atgctggtga gagcatccac
     4621 atcatccgtg ggacaaaagg gacgtacatc cgtaccagtg atggacggat ctttgctgtc
     4681 cgggcaactg gcaaaccaaa ggttcctgaa gatggtcgga tggctgcctc aggttcccag
     4741 ggaccttctt gcgagtccac aagcaacggc agacacagtg cctcatcacc caaagccccc
     4801 gaccctgagg ggctggccag gcccgtctct cctgacagcc cagagatcat cagtgagctt
     4861 cagcagtatg cagatgtggc tgctgcccgg gaatcccgtc agagctcccc aagcaccaat
     4921 gccgccctgc ctggcccccc ggcccaactt atggacagca gtgctgttcc cgggacagct
     4981 ctcggaactg agcctcgact agggggtcat tgcctcaata gttccctctt ggtgactggc
     5041 cagccctgtg gtgacaggca cccagtgctg gacttaaggg gccacaagcg aaagttggcc
     5101 acaccacctg ctgcccagga gtcatcccgc cggcggtcca ggaagggtca tctgccagcc
     5161 cccgtgcagc cgtatgaaca cgggtatcca gtctctggcg ggtttgccat gccacccgtc
     5221 tccttaaacc ataacctcac cacccccttc acctcccagg ctggggagaa ctccctgttt
     5281 atgggcagta ccccctccta ctaccagctg tccaatttgc tggcagatgc ccgcctggtg
     5341 tttccagtga ctactgaccc tctggtgcca gcaggccccg tcagttcctc ttccacggct
     5401 acctcagtca ctgccagcaa cccctccttc atgctcaacc cttctgtgcc agggatacta
     5461 cccagctatt cactcccatt ctcacagcca ctcctgtccg agccgaggat gtttgcgcct
     5521 tttccttccc ctgtcttgcc cagcaacctt tcgcggggca tgtctatcta tccaggctac
     5581 atgtccccac atgcaggcta cccagctggt ggcctcctac ggtcccaggt gcctccattt
     5641 gactctcatg aggttgccga ggttgggttc agctccaatg atgatgagga taaagacgat
     5701 gatgtgatag aggtcactgg gaaacaccca gctttcttgt acaaagtggt tgatatggat
     5761 agtgctggta gtgctggtag tgctggtaaa gaaaccgctg ctgctaaatt cgaacgccag
     5821 cacatggaca gcggagccgg cgcaggagcc ggcgctgacg cgcctgacta caaagacgat
     5881 gacgacaagg gagattacaa ggatgacgat gacaaaggcg cgtcgatgga cgagaagacc
     5941 accggctggc ggggcggcca cgtggtggag ggcctggccg gcgagctgga gcagctgcgg
     6001 gccaggctgg agcaccaccc tcagggccag cgggagccct aaagtaccgg ttaagtcgac
     6061 aatcaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6121 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6181 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6241 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6301 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6361 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6421 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     6481 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6541 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6601 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6661 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6721 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     6781 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6841 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6901 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6961 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     7021 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7081 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7141 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7201 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7261 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7321 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     7381 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     7441 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     7501 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     7561 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7621 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7681 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7741 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7801 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     7861 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7921 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7981 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     8041 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     8101 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8161 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8221 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8281 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8341 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8401 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8461 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8521 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8581 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8641 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8701 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8761 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8821 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8881 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8941 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9001 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9061 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9121 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9181 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9241 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9301 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9361 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9421 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9481 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     9541 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     9601 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9661 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9721 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9781 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9841 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9901 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     9961 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    10021 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    10081 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    10141 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
    10201 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
    10261 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
    10321 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
    10381 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
    10441 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
    10501 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
    10561 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
    10621 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
    10681 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
    10741 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
    10801 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
    10861 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
    10921 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
    10981 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
    11041 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
    11101 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
    11161 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
    11221 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
    11281 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
    11341 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
    11401 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
    11461 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
    11521 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
    11581 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
    11641 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    11701 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    11761 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    11821 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    11881 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccatg
    11941 cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
    12001 gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc ctaactccgc
    12061 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    12121 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    12181 gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc accggagctt
    12241 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
    12301 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
    12361 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
    12421 gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg gaccacgccg
    12481 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
    12541 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
    12601 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
    12661 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
    12721 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
    12781 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
    12841 agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
    12901 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
    12961 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
    13021 ggacagcaga gatccacttt ggcgccgg
//
