LOCUS       sequence-494860-       12799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct for C-terminal S 
            protein-Flag-Streptavidin binding peptide (SFB)-tagged RAD54L2 
            D1-86.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12799)
  AUTHORS   Zhang H, Xiong Y, Sun Y, Park JM, Su D, Feng X, Keast S, Tang M, 
            Huang M, Wang C, Srivastava M, Yang C, Zhu D, Chen Z, Li S, Yin L, 
            Pommier Y, Chen J
  TITLE     RAD54L2-mediated DNA damage avoidance pathway specifically preserves
            genome integrity in response to topoisomerase 2 poisons.
  JOURNAL   Sci Adv. 2023 Dec 8;9(49):eadi6681. doi: 10.1126/sciadv.adi6681. 
            Epub 2023 Dec 6.
  PUBMED    38055811
REFERENCE   2  (bases 1 to 12799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-12-8"; volume: "9(49):eadi6681. doi"; pages: " 
            10.1126/sciadv.adi6681. Epub 2023 Dec 6"
COMMENT     Sequence Label: pLEX307-RAD54L2-D1-86-SFB
FEATURES             Location/Qualifiers
     source          1..12799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5477..5501)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5539..5583
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             5617..5640
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5644..5667
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5677..5790
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     misc_feature    5832..6420
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5885..5905)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6303..6314)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6492..6725
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6803..6924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6840..6859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6894..6913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6964..7099
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7119..7138)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7120..7138)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7148..7165)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7148..7164)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7157..7179)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7306..7761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7393..7412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7603..7624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7787..7891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7892..7960,7961..8752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8110..8129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8923..9511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9412..9431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9665..9682
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9799..9820
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9835..9840,9841..9858,9859..9865)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9873..9889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9878..9900
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9897..9913
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9897..9913
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9932..9952
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9934..9952
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9980..10206
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10207..10387
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10434..10559
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11052..11285
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11470..11514
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11745..11955
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11767..11963
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11814..11949
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11995..12597
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11995..12014)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12491..12511
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12640..12757
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggcttc
     1321 accatggcca tgcgccacca aggcaagaac ctagcctccg aggaccccaa aaagaagaga
     1381 gctcagaagc cctcccacat gagaagaaac atacgaaagc tactccggga ggatcaattg
     1441 gagcctgtta ccaaagcagc acagcaagaa gagttggaaa gaaggaagcg cctggagcag
     1501 cagaggaaag attatgcagc ccctattcct actgttccgc tggagttcct ccctgaggaa
     1561 attgctttaa gggcaagtga cggtccccaa ctgcctcctc gggtcttggc ccaggaagtc
     1621 atttgtttgg acagtagcag tggcagtgag gatgaaaaaa gcagtcgaga tgaggtgatt
     1681 gaactgagct ctggagagga ggacactctg cacattgtgg acagcagtga atctgtcagt
     1741 gaagatgatg aggaagaaga gaagggtggc acccatgtca atgatgtctt aaaccagcgt
     1801 gacgcccttg ggcgggtcct tgtcaaccta aaccaccctc cagaggagga aaatgtcttc
     1861 cttgccccac agttggcacg ggctgtgaaa cctcatcaga ttggcgggat ccggttcctt
     1921 tacgataacc tagtggagtc tctggagagg tttaagacca gcagtggctt tggctgtatt
     1981 ctggcccaca gcatgggtct ggggaaaact ttgcaagtga tctctttcat cgacgtcctc
     2041 ttccgccaca cgccagccaa aacagtcctt gccattgtgc cggttaatac tcttcagaat
     2101 tggctggcag agttcaacat gtggcttcca cctcctgaag ccctcccggc tgacaacaag
     2161 cctgaagaag tccagcctcg gttctttaaa gttcacatct tgaatgatga gcacaagacg
     2221 atggcatctc gtgctaaagt gatggctgat tgggtgtcag agggtggcgt gctgctgatg
     2281 gggtacgaga tgtacagact cctcactctg aagaaatcat ttgccacagg tagaccgaag
     2341 aaaaccaaga agcgttctca cccagtcatc attgatctag atgaggaaga tcggcagcag
     2401 gagtttcgga gagagtttga gaaggcttta tgccgccctg gccctgatgt agtaatctgt
     2461 gatgagggac accgcatcaa aaactgccag gccagcacct cacaggctct gaagaatatc
     2521 cgctctcgcc gccgggtggt gctgactggg taccctctgc aaaacaacct cattgagtac
     2581 tggtgcatgg tggactttgt gcgcccagac ttccttggca cccggcagga gttcagcaac
     2641 atgtttgaac gccctatcct gaatgggcaa tgtattgaca gcacacctca ggacgtccgc
     2701 ctcatgcggt accggagcca tgtcctgcac agtcttctgg agggctttgt gcagaggaga
     2761 ggccacactg tgctgaagat tcatctccct gccaaggaag aaaatgtgat ccttgtgcgg
     2821 ctctccaaga tccagcgaga tttgtataca cagttcatgg atcgcttccg ggactgtggt
     2881 agcagcggtt ggctggggct gaaccccctt aaggcattct gtgtgtgttg caagatctgg
     2941 aatcaccctg atgtgctgta tgaagccctt cagaaggaga gcttggccaa tgagcaggac
     3001 ctagacgtgg aagaacttgg ctctgcaggg accagtgccc gctgtccacc acagggaaca
     3061 aaaggcaagg gagaggatag caccttggct tcctcgatgg gagaggcaac caatagcaag
     3121 ttcctacagg gcgttggctt caaccctttc caggagcgag gcaacaacat tgtcacatat
     3181 gaatgggcca aggaccttct gactaattac cagactggag tcctagaaaa ctctcccaag
     3241 atggtactgc ttttccacct gattgaggaa agtgtgaagc ttggggacaa gatccttgtg
     3301 tttagccaga gtctttccac cttggctctc atcgaggaat tccttggaaa acgagaagta
     3361 ccctgtccac ctggtaccga ggggcaagga gcacagaagt gggttcgaaa catcagctac
     3421 ttccggctag atggtagcac ccctgccttt gagagggagc ggcttattaa tcagttcaat
     3481 gatcccagca acctcaccac ctggctgttc cttctctcta caagggccgg atgcttgggt
     3541 gtgaatctga ttggtgccaa ccgagtggtg gtgtttgatg cttcctggaa cccttgccat
     3601 gatgcccagg cagtatgtcg ggtataccgt tatggccaga aaaagccctg ttacatctat
     3661 cgccttgtgg ctgattacac tctagaaaag aagatctatg accgtcagat ttccaagcag
     3721 ggcatgtcag atcgggtggt ggatgatcta aatccaatgc tgaacttcac acggaaagag
     3781 gtggaaaacc tactgcactt tgttgagaag gagccagctc cccaagtttc cttgaacgta
     3841 aaggggatca aggagtcagt cctgcaactg gcctgtctga agtaccctca cctcatcacc
     3901 aaggagcctt tcgagcatga gtcattgctc ttgaaccgaa aggatcacaa gctaaccaag
     3961 gctgagaaaa aagcagcaaa gaaaagctat gaggaagaca aacgcacatc agtcccctat
     4021 acccgcccat cgtatgcgca gtattaccct gccagcgatc agagcctgac cagcatcccc
     4081 gccttcagcc agagaaactg gcagccaact ttgaagggtg atgaaaagcc tgtggccagt
     4141 gttcgtcctg tgcagtccac ccccatcccc atgatgcccc ggcatgtccc attgggagga
     4201 agtgtaagct ctgcctccag cacaaatcca tccatgaact ttcccatcaa ctacttgcag
     4261 cgtgcaggag tccttgtgca gaaggtggtc accacgacag atattgttat tcctggactc
     4321 aacagctcca cagatgtaca ggcaagaatt aatgctggtg agagcatcca catcatccgt
     4381 gggacaaaag ggacgtacat ccgtaccagt gatggacgga tctttgctgt ccgggcaact
     4441 ggcaaaccaa aggttcctga agatggtcgg atggctgcct caggttccca gggaccttct
     4501 tgcgagtcca caagcaacgg cagacacagt gcctcatcac ccaaagcccc cgaccctgag
     4561 gggctggcca ggcccgtctc tcctgacagc ccagagatca tcagtgagct tcagcagtat
     4621 gcagatgtgg ctgctgcccg ggaatcccgt cagagctccc caagcaccaa tgccgccctg
     4681 cctggccccc cggcccaact tatggacagc agtgctgttc ccgggacagc tctcggaact
     4741 gagcctcgac tagggggtca ttgcctcaat agttccctct tggtgactgg ccagccctgt
     4801 ggtgacaggc acccagtgct ggacttaagg ggccacaagc gaaagttggc cacaccacct
     4861 gctgcccagg agtcatcccg ccggcggtcc aggaagggtc atctgccagc ccccgtgcag
     4921 ccgtatgaac acgggtatcc agtctctggc gggtttgcca tgccacccgt ctccttaaac
     4981 cataacctca ccaccccctt cacctcccag gctggggaga actccctgtt tatgggcagt
     5041 accccctcct actaccagct gtccaatttg ctggcagatg cccgcctggt gtttccagtg
     5101 actactgacc ctctggtgcc agcaggcccc gtcagttcct cttccacggc tacctcagtc
     5161 actgccagca acccctcctt catgctcaac ccttctgtgc cagggatact acccagctat
     5221 tcactcccat tctcacagcc actcctgtcc gagccgagga tgtttgcgcc ttttccttcc
     5281 cctgtcttgc ccagcaacct ttcgcggggc atgtctatct atccaggcta catgtcccca
     5341 catgcaggct acccagctgg tggcctccta cggtcccagg tgcctccatt tgactctcat
     5401 gaggttgccg aggttgggtt cagctccaat gatgatgagg ataaagacga tgatgtgata
     5461 gaggtcactg ggaaacaccc agcttttttg tacaaagtgg ttgatatgga tagtgctggt
     5521 agtgctggta gtgctggtaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac
     5581 agcggagccg gcgcaggagc cggcgctgac gcgcctgact acaaagacga tgacgacaag
     5641 ggagattaca aggatgacga tgacaaaggc gcgtcgatgg acgagaagac caccggctgg
     5701 cggggcggcc acgtggtgga gggcctggcc ggcgagctgg agcagctgcg ggccaggctg
     5761 gagcaccacc ctcagggcca gcgggagccc taaagtaccg gttaagtcga caatcaacgc
     5821 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5881 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5941 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6001 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6061 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6121 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6181 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6241 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6301 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6361 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6421 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6481 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     6541 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6601 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6661 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6721 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     6781 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     6841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6901 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     6961 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7021 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7081 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     7141 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     7201 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     7261 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     7321 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7381 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7441 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7501 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7561 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7621 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7681 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7741 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7801 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7861 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7921 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7981 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8041 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8101 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8161 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8221 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8281 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8341 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8401 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8461 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8521 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8581 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8641 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8701 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8761 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8821 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8881 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8941 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9001 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9061 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9121 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9181 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9241 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9301 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9361 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9421 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9481 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9541 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9601 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9661 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9721 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     9781 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     9841 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     9901 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     9961 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
    10021 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
    10081 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
    10141 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
    10201 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    10261 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    10321 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    10381 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
    10441 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
    10501 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
    10561 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
    10621 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
    10681 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
    10741 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
    10801 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
    10861 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
    10921 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
    10981 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
    11041 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
    11101 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
    11161 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
    11221 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
    11281 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
    11341 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
    11401 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
    11461 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
    11521 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
    11581 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
    11641 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat gcattgcatc
    11701 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
    11761 aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
    11821 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
    11881 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    11941 ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct taccatgacc
    12001 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
    12061 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
    12121 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
    12181 aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc
    12241 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
    12301 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
    12361 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
    12421 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
    12481 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
    12541 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc cagttaacga
    12601 attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
    12661 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
    12721 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
    12781 agatccactt tggcgccgg
//
