LOCUS       sequence-494863-       12433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct for C-terminal S 
            protein-Flag-Streptavidin binding peptide (SFB)-tagged RAD54L2 
            D87-29.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Zhang H, Xiong Y, Sun Y, Park JM, Su D, Feng X, Keast S, Tang M, 
            Huang M, Wang C, Srivastava M, Yang C, Zhu D, Chen Z, Li S, Yin L, 
            Pommier Y, Chen J
  TITLE     RAD54L2-mediated DNA damage avoidance pathway specifically preserves
            genome integrity in response to topoisomerase 2 poisons.
  JOURNAL   Sci Adv. 2023 Dec 8;9(49):eadi6681. doi: 10.1126/sciadv.adi6681. 
            Epub 2023 Dec 6.
  PUBMED    38055811
REFERENCE   2  (bases 1 to 12433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-12-8"; volume: "9(49):eadi6681. doi"; pages: " 
            10.1126/sciadv.adi6681. Epub 2023 Dec 6"
COMMENT     Sequence Label: pLEX307-RAD54L2-D87-291-SFB
FEATURES             Location/Qualifiers
     source          1..12433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5111..5135)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5173..5217
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             5251..5274
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5278..5301
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5311..5424
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     misc_feature    5466..6054
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5519..5539)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5937..5948)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6126..6359
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6437..6558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6474..6493)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6528..6547
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6598..6733
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6753..6772)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6754..6772)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6782..6799)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6782..6798)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6791..6813)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6940..7395
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7027..7046)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7237..7258
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7421..7525
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7526..7594,7595..8386)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7744..7763)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8557..9145
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9046..9065
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9299..9316
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9433..9454
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9469..9474,9475..9492,9493..9499)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9507..9523
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9512..9534
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9531..9547
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9531..9547
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9566..9586
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9568..9586
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9614..9840
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9841..10021
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10068..10193
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10686..10919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11104..11148
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11379..11589
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11401..11597
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11448..11583
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11629..12231
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11629..11648)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12125..12145
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12274..12391
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggcttc
     1321 accatgtcag acgaatctgc ctcagggagc gatccagacc tggacccgga cgtggagctg
     1381 gaggatgcgg aagaggagga ggaggaggag gaggtggcag tggaggagtg tgacagggat
     1441 gatgaagaag acctgctgga tgacccatcc ctggaaggca tgtgtggcac tgagcatgcc
     1501 cagttgggag aagatgggca gcagccgccg cggtgcactt caactacctc atctcagtct
     1561 gagccttcag agcagcttag gaggtttaag accagcagtg gctttggctg tattctggcc
     1621 cacagcatgg gtctggggaa aactttgcaa gtgatctctt tcatcgacgt cctcttccgc
     1681 cacacgccag ccaaaacagt ccttgccatt gtgccggtta atactcttca gaattggctg
     1741 gcagagttca acatgtggct tccacctcct gaagccctcc cggctgacaa caagcctgaa
     1801 gaagtccagc ctcggttctt taaagttcac atcttgaatg atgagcacaa gacgatggca
     1861 tctcgtgcta aagtgatggc tgattgggtg tcagagggtg gcgtgctgct gatggggtac
     1921 gagatgtaca gactcctcac tctgaagaaa tcatttgcca caggtagacc gaagaaaacc
     1981 aagaagcgtt ctcacccagt catcattgat ctagatgagg aagatcggca gcaggagttt
     2041 cggagagagt ttgagaaggc tttatgccgc cctggccctg atgtagtaat ctgtgatgag
     2101 ggacaccgca tcaaaaactg ccaggccagc acctcacagg ctctgaagaa tatccgctct
     2161 cgccgccggg tggtgctgac tgggtaccct ctgcaaaaca acctcattga gtactggtgc
     2221 atggtggact ttgtgcgccc agacttcctt ggcacccggc aggagttcag caacatgttt
     2281 gaacgcccta tcctgaatgg gcaatgtatt gacagcacac ctcaggacgt ccgcctcatg
     2341 cggtaccgga gccatgtcct gcacagtctt ctggagggct ttgtgcagag gagaggccac
     2401 actgtgctga agattcatct ccctgccaag gaagaaaatg tgatccttgt gcggctctcc
     2461 aagatccagc gagatttgta tacacagttc atggatcgct tccgggactg tggtagcagc
     2521 ggttggctgg ggctgaaccc ccttaaggca ttctgtgtgt gttgcaagat ctggaatcac
     2581 cctgatgtgc tgtatgaagc ccttcagaag gagagcttgg ccaatgagca ggacctagac
     2641 gtggaagaac ttggctctgc agggaccagt gcccgctgtc caccacaggg aacaaaaggc
     2701 aagggagagg atagcacctt ggcttcctcg atgggagagg caaccaatag caagttccta
     2761 cagggcgttg gcttcaaccc tttccaggag cgaggcaaca acattgtcac atatgaatgg
     2821 gccaaggacc ttctgactaa ttaccagact ggagtcctag aaaactctcc caagatggta
     2881 ctgcttttcc acctgattga ggaaagtgtg aagcttgggg acaagatcct tgtgtttagc
     2941 cagagtcttt ccaccttggc tctcatcgag gaattccttg gaaaacgaga agtaccctgt
     3001 ccacctggta ccgaggggca aggagcacag aagtgggttc gaaacatcag ctacttccgg
     3061 ctagatggta gcacccctgc ctttgagagg gagcggctta ttaatcagtt caatgatccc
     3121 agcaacctca ccacctggct gttccttctc tctacaaggg ccggatgctt gggtgtgaat
     3181 ctgattggtg ccaaccgagt ggtggtgttt gatgcttcct ggaacccttg ccatgatgcc
     3241 caggcagtat gtcgggtata ccgttatggc cagaaaaagc cctgttacat ctatcgcctt
     3301 gtggctgatt acactctaga aaagaagatc tatgaccgtc agatttccaa gcagggcatg
     3361 tcagatcggg tggtggatga tctaaatcca atgctgaact tcacacggaa agaggtggaa
     3421 aacctactgc actttgttga gaaggagcca gctccccaag tttccttgaa cgtaaagggg
     3481 atcaaggagt cagtcctgca actggcctgt ctgaagtacc ctcacctcat caccaaggag
     3541 cctttcgagc atgagtcatt gctcttgaac cgaaaggatc acaagctaac caaggctgag
     3601 aaaaaagcag caaagaaaag ctatgaggaa gacaaacgca catcagtccc ctatacccgc
     3661 ccatcgtatg cgcagtatta ccctgccagc gatcagagcc tgaccagcat ccccgccttc
     3721 agccagagaa actggcagcc aactttgaag ggtgatgaaa agcctgtggc cagtgttcgt
     3781 cctgtgcagt ccacccccat ccccatgatg ccccggcatg tcccattggg aggaagtgta
     3841 agctctgcct ccagcacaaa tccatccatg aactttccca tcaactactt gcagcgtgca
     3901 ggagtccttg tgcagaaggt ggtcaccacg acagatattg ttattcctgg actcaacagc
     3961 tccacagatg tacaggcaag aattaatgct ggtgagagca tccacatcat ccgtgggaca
     4021 aaagggacgt acatccgtac cagtgatgga cggatctttg ctgtccgggc aactggcaaa
     4081 ccaaaggttc ctgaagatgg tcggatggct gcctcaggtt cccagggacc ttcttgcgag
     4141 tccacaagca acggcagaca cagtgcctca tcacccaaag cccccgaccc tgaggggctg
     4201 gccaggcccg tctctcctga cagcccagag atcatcagtg agcttcagca gtatgcagat
     4261 gtggctgctg cccgggaatc ccgtcagagc tccccaagca ccaatgccgc cctgcctggc
     4321 cccccggccc aacttatgga cagcagtgct gttcccggga cagctctcgg aactgagcct
     4381 cgactagggg gtcattgcct caatagttcc ctcttggtga ctggccagcc ctgtggtgac
     4441 aggcacccag tgctggactt aaggggccac aagcgaaagt tggccacacc acctgctgcc
     4501 caggagtcat cccgccggcg gtccaggaag ggtcatctgc cagcccccgt gcagccgtat
     4561 gaacacgggt atccagtctc tggcgggttt gccatgccac ccgtctcctt aaaccataac
     4621 ctcaccaccc ccttcacctc ccaggctggg gagaactccc tgtttatggg cagtaccccc
     4681 tcctactacc agctgtccaa tttgctggca gatgcccgcc tggtgtttcc agtgactact
     4741 gaccctctgg tgccagcagg ccccgtcagt tcctcttcca cggctacctc agtcactgcc
     4801 agcaacccct ccttcatgct caacccttct gtgccaggga tactacccag ctattcactc
     4861 ccattctcac agccactcct gtccgagccg aggatgtttg cgccttttcc ttcccctgtc
     4921 ttgcccagca acctttcgcg gggcatgtct atctatccag gctacatgtc cccacatgca
     4981 ggctacccag ctggtggcct cctacggtcc caggtgcctc catttgactc tcatgaggtt
     5041 gccgaggttg ggttcagctc caatgatgat gaggataaag acgatgatgt gatagaggtc
     5101 actgggaaac acccagcttt cttgtacaaa gtggttgata tggatagtgc tggtagtgct
     5161 ggtagtgctg gtaaagaaac cgctgctgct aaattcgaac gccagcacat ggacagcgga
     5221 gccggcgcag gagccggcgc tgacgcgcct gactacaaag acgatgacga caagggagat
     5281 tacaaggatg acgatgacaa aggcgcgtcg atggacgaga agaccaccgg ctggcggggc
     5341 ggccacgtgg tggagggcct ggccggcgag ctggagcagc tgcgggccag gctggagcac
     5401 caccctcagg gccagcggga gccctaaagt accggttaag tcgacaatca acgcgttaag
     5461 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5521 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5581 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5641 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5701 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5761 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5821 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5881 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5941 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6001 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     6061 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6121 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     6181 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6241 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6301 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6361 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     6421 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6481 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6541 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     6601 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6661 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     6721 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     6781 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     6841 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6901 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     6961 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     7021 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7081 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     7141 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     7201 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     7261 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     7321 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     7381 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7441 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7501 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7561 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7621 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7681 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7741 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     7801 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7861 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7921 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7981 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     8041 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     8101 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     8161 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8221 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8281 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8341 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     8401 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     8461 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8521 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8581 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8641 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8701 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8761 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8821 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8881 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8941 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     9001 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     9061 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     9121 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     9181 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9241 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     9301 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     9361 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     9421 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     9481 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     9541 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     9601 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     9661 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     9721 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     9781 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     9841 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     9901 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     9961 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
    10021 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
    10081 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
    10141 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
    10201 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
    10261 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
    10321 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
    10381 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
    10441 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
    10501 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
    10561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    10621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    10681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    10741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    10801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    10861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    10921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    10981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    11041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    11101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    11161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    11221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    11281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt
    11341 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    11401 tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    11461 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    11521 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    11581 gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat gaccgagtac
    11641 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
    11701 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
    11761 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
    11821 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
    11881 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
    11941 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
    12001 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
    12061 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
    12121 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
    12181 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta acgaattcta
    12241 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
    12301 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
    12361 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
    12421 actttggcgc cgg
//
