LOCUS       sequence-494873-        8318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of OsTIR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Hubbe P, Sharma C, Pajonk O, Peters N, Guschtschin-Schmidt N, 
            Friemel N, Schuck S
  TITLE     An optimised system for rapid auxin-inducible protein degradation in
            budding yeast
  JOURNAL   bioRxiv 2025.09.24.678194
REFERENCE   2  (bases 1 to 8318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.24.678194"
COMMENT     Sequence Label: pRS303K-Pgpd-OsTIR1(F74G)
FEATURES             Location/Qualifiers
     source          1..8318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..47)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     147..166
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        313..500
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     gene            complement(771..2127)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(771..968)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(974..1783)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1697..1716
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1784..2127)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        2212..2855
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     2749..2772
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             2873..4600
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein, 
                     mutated for use in the AID2 system of inducible degradation
                     (Yesbolatova et al., 2020)"
                     /label=OsTIR1(F74G)
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHGADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     terminator      4607..4853
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4623..4641)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(5378..5833)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5515..5536)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5727..5746
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(5810..6097)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSSKLTLTKGNKSWYRAPPSNSPYSESYYNSLAVVLQRRD
                     WENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     5959..5981
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5973..5990
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5974..5990
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5997..6016
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5997..6015
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6054..6072)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6093..6109)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6093..6109)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6106..6128)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6117..6133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6141..6147,6148..6165,6166..6171))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6186..6207
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6324..6341)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6495..7083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6575..6594)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7254..8045,8046..8114))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7877..7896
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8115..8219)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8287..8305
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
       61 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      121 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
      181 taaattcccg ttttaagagc ttggtgagcg ctaggagtca ctgccaggta tcgtttgaac
      241 acggcattag tcagggaagt cataacacag tcctttcccg caattttctt tttctattac
      301 tcttggcctc ctctagtaca ctctatattt ttttatgcct cggtaatgat tttcattttt
      361 ttttttccac ctagcggatg actctttttt tttcttagcg attggcatta tcacataatg
      421 aattatacat tatataaagt aatgtgattt cttcgaagaa tatactaaaa aatgagcagg
      481 caagataaac gaaggcaaag atgacagagc agaaagccct agtaaagcgt attacaaatg
      541 aaaccaagat tcagattgcg atctctttaa agggtggtcc cctagcgata gagcactcga
      601 tcttcccaga aaaagaggca gaagcagtag caaaacaggc cacacaatcg caagtgatta
      661 acgtccacac aggtataggg tttctggacc atatgataca tgctctggat catcgatgaa
      721 ttcgagctcg ttttcgacac tggatggcgg cgttagtatc gaatcgacag cagtatagcg
      781 accagcattc acatacgatt gacgcatgat attactttct gcgcacttaa cttcgcatct
      841 gggcagatga tgtcgaggcg aaaaaaaata taaatcacgc taacatttga ttaaaataga
      901 acaactacaa tataaaaaaa ctatacaaat gacaagttct tgaaaacaag aatcttttta
      961 ttgtcagtac tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1021 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1081 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1141 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1201 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     1261 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1321 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1381 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1441 ctgaatcagg atattcttct aatacctgga atgctgtttt gccggggatc gcagtggtga
     1501 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1561 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1621 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1681 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1741 aatttaatcg cggcctcgaa acgtgagtct tttccttacc catggttgtt tatgttcgga
     1801 tgtgatgtga gaactgtatc ctagcaagat tttaaaagga agtatatgaa agaagaacct
     1861 cagtggcaaa tcctaacctt ttatatttct ctacaggggc gcggcgtggg gacaattcaa
     1921 cgcgtctgtg aggggagcgt ttccctgctc gcaggtctgc agcgaggagc cgtaattttt
     1981 gcttcgcgcc gtgcggccat caaaatgtat ggatgcaaat gattatacat ggggatgtat
     2041 gggctaaatg tacgggcgac agtcacatca tgcccctgag ctgcgcacgt caagactgtc
     2101 aaggagggta ttctgggcct ccatgtcgct ggccgggtga cccggcgggg acgaggcaag
     2161 ctaaacagat ctggcgcgcc ttaattaacc cggggatccg agctcagttt atcattatca
     2221 atactcgcca tttcaaagaa tacgtaaata attaatagta gtgattttcc taactttatt
     2281 tagtcaaaaa attagccttt taattctgct gtaacccgta catgcccaaa atagggggcg
     2341 ggttacacag aatatataac atcgtaggtg tctgggtgaa cagtttattc ctggcatcca
     2401 ctaaatataa tggagcccgc tttttaagct ggcatccaga aaaaaaaaga atcccagcac
     2461 caaaatattg ttttcttcac caaccatcag ttcataggtc cattctctta gcgcaactac
     2521 agagaacagg ggcacaaaca ggcaaaaaac gggcacaacc tcaatggagt gatgcaacct
     2581 gcctggagta aatgatgaca caaggcaatt gacccacgca tgtatctatc tcattttctt
     2641 acaccttcta ttaccttctg ctctctctga tttggaaaaa gctgaaaaaa aaggttgaaa
     2701 ccagttccct gaaattattc ccctacttga ctaataagta tataaagacg gtaggtattg
     2761 attgtaattc tgtaaatcta tttcttaaac ttcttaaatt ctacttttat agttagtctt
     2821 ttttttagtt ttaaaacacc agaacttagt ttcgacggat tctagaacta gtatgactta
     2881 ttttcctgaa gaagttgtag aacacatatt ttccttttta cctgctcaaa gagatagaaa
     2941 caccgtctcc ttggtctgta aggtttggta cgaaatcgaa agattgtcta gaagaggtgt
     3001 ttttgttggt aattgttacg cagttagagc tggtagagtt gctgcaagat tcccaaacgt
     3061 tagagcattg actgttaaag gtaaacctca tggtgctgat ttcaatttgg ttccacctga
     3121 ttggggtggt tatgctggtc catggattga agctgcagct agaggttgtc acggtttgga
     3181 agaattgaga atgaagagaa tggttgtttc tgatgaatca ttggaattgt tagcaagatc
     3241 ttttcctaga ttcagagctt tggttttgat ctcatgtgaa ggtttttcca ccgatggttt
     3301 agcagctgtt gcttcacatt gtaagttgtt gagagaattg gatttgcaag aaaacgaagt
     3361 tgaagataga ggtccaagat ggttatcttg ttttcctgat tcctgtacaa gtttggtttc
     3421 tttgaacttc gcatgtatca agggtgaagt taacgctggt agtttggaaa gattagtttc
     3481 tagatcacca aatttgagat ctttgagatt gaacagatcc gttagtgttg ataccttggc
     3541 aaagatcttg ttgagaactc ctaatttgga agatttgggt actggtaact tgacagatga
     3601 tttccaaaca gaatcatact tcaagttgac ctccgctttg gaaaagtgta agatgttgag
     3661 atctttgtca ggtttttggg atgcaagtcc agtttgtttg tcttttatat atcctttgtg
     3721 tgctcaattg actggtttga atttgtctta cgcaccaact ttggatgcta gtgatttgac
     3781 aaagatgatc tctagatgtg ttaagttgca aagattgtgg gttttggatt gtatctcaga
     3841 taaaggtttg caagttgttg cttcttcttg taaggatttg caagaattga gagtttttcc
     3901 aagtgatttc tatgttgcag gttactctgc tgttacagaa gaaggtttag ttgcagtttc
     3961 attgggttgt cctaagttga actccttgtt gtacttctgt catcaaatga ccaacgcagc
     4021 tttggttact gttgctaaaa attgtccaaa cttcactaga ttcagattgt gtatcttgga
     4081 accaggtaaa cctgatgttg ttacatcaca acctttagat gaaggttttg gtgcaatcgt
     4141 tagagagtgt aagggtttgc aaagattgtc catcagtggt ttgttgacag ataaggtttt
     4201 tatgtacatc ggtaaatacg ctaagcaatt ggaaatgttg tcaatcgcat tcgctggtga
     4261 ctccgataag ggtatgatgc atgttatgaa cggttgtaag aacttaagaa agttggaaat
     4321 cagagattct ccatttggtg acgcagcttt gttgggtaat ttcgcaagat atgaaacaat
     4381 gagatctttg tggatgtcca gttgtaacgt taccttgaaa ggttgtcaag ttttagcttc
     4441 aaagatgcca atgttgaacg ttgaagttat taacgaaaga gatggttcca atgaaatgga
     4501 agaaaaccat ggtgacttgc ctaaagttga aaagttatac gtctacagaa caaccgcagg
     4561 tgccagagat gatgctccta acttcgtcaa gattttatga ctcgagtcat gtaattagtt
     4621 atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt
     4681 agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg
     4741 ttatttatat ttcaaatttt tctttttttc tgtacagacg cgtgtacgca tgtaacatta
     4801 tactgaaaac cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgcggccggt
     4861 cgacctgcag cgtacgcagt tgtcgaactt ggtttgcaaa gggagaaagt aggagatctc
     4921 tcttgcgaga tgatcccgca ttttcttgaa agctttgcag aggctagcag aattaccctc
     4981 cacgttgatt gtctgcgagg caagaatgat catcaccgta gtgagagtgc gttcaaggct
     5041 cttgcggttg ccataagaga agccacctcg cccaatggta ccaacgatgt tccctccacc
     5101 aaaggtgttc ttatgtagtg acaccgatta tttaaagctg cagcatacga tatatataca
     5161 tgtgtatata tgtataccta tgaatgtcag taagtatgta tacgaacagt atgatactga
     5221 agatgacaag gtaatgcatc attctatacg tgtcattctg aacgaggcgc gctttccttt
     5281 tttctttttg ctttttcttt ttttttctct tgaactcgac ggatctatgc ggtgtgaaat
     5341 accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg
     5401 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     5461 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     5521 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     5581 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     5641 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     5701 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     5761 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     5821 ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     5881 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     5941 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
     6001 acgactcact atagggcgaa ttggaggggg gggcccggta ccagcttttg ttccctttag
     6061 tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6121 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6181 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6241 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6301 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6361 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6421 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6481 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6541 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6601 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6661 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6721 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6781 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6841 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6901 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6961 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7021 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7081 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7141 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7201 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7261 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7321 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7381 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7441 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7501 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7561 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7621 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7681 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7741 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7801 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7861 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7921 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7981 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8041 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8101 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8161 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8221 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     8281 tataaaaata ggcgtatcac gaggcccttt cgtctcgc
//
