LOCUS       sequence-494874-       11816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct for N-terminal S 
            protein-Flag-Streptavidin binding peptide (SFB)-tagged SLX4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Zhang H, Chen Z, Ye Y, Ye Z, Cao D, Xiong Y, Srivastava M, Feng X, 
            Tang M, Wang C, Tainer JA, Chen J
  TITLE     SLX4IP acts with SLX4 and XPF-ERCC1 to promote interstrand crosslink
            repair.
  JOURNAL   Nucleic Acids Res. 2019 Nov 4;47(19):10181-10201. doi: 
            10.1093/nar/gkz769.
  PUBMED    31495888
REFERENCE   2  (bases 1 to 11816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-11-4- 4"; volume: "47"; issue: 
            "19"; pages: "10181-10201"
COMMENT     Sequence Label: SFB-SLX4
FEATURES             Location/Qualifiers
     source          1..11816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        22..325
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        326..529
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     480..500
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             589..633
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             667..690
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             694..717
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             727..840
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    913..937
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6446..6470)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6653..6670)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(6732..6752)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6737..7066
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6917..7052
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7076..7675
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7076..7095)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7572..7592
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7896..8365
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8495..8512)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8666..9254)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8746..8765)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9414..10205,10206..10274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10037..10056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10275..10379)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             10410..10879
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    10944..11143
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             11344..11760
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     11690..11712
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     11728..11744
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
       61 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      121 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      181 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      241 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      301 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      361 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      421 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      481 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
      541 ccgtcagatc cgctagcgct accggactca gatctcgagg ccaccatgaa agaaaccgct
      601 gctgctaaat tcgaacgcca gcacatggac agcggagccg gcgcaggagc cggcgctgac
      661 gcgcctgact acaaagacga tgacgacaag ggagattaca aggatgacga tgacaaaggc
      721 gcgtcgatgg acgagaagac caccggctgg cggggcggcc acgtggtgga gggcctggcc
      781 ggcgagctgg agcagctgcg ggccaggctg gagcaccacc ctcagggcca gcgggagccc
      841 tccggcggct gcaagctggg cggcgcgcct gaattctgca gtcgacggta ccgcgggccc
      901 atcgctcgag agacaagttt gtacaaaaaa gcaggcttca tgaaactgag tgtgaatgag
      961 gctcagctag gcttctactt gggttcactt tctcatctgt ctgcctgtcc tgggattgac
     1021 cctcgctcct ctgaagacca gcctgaaagc cttaaaactg gtcagatgat ggatgagtct
     1081 gatgaggact ttaaagaact ctgcgctagc tttttccaaa gggtgaaaaa acatggaatc
     1141 aaggaagtgt caggagaaag gaagacacaa aaggctgcct caaacggcac tcagataaga
     1201 agcaaattga aaaggaccaa acaaactgct accaagacca aaacccttca aggccctgca
     1261 gagaagaaac ctccgtctgg cagccaggcc cctaggacta aaaagcaaag ggtaaccaaa
     1321 tggcaagcaa gtgaaccggc ccactctgtg aatggggagg ggggtgtgct tgcctctgct
     1381 ccagatccac ctgtgctccg ggaaacagca caaaacaccc agacgggtaa ccagcaagaa
     1441 ccatcgccaa acctttccag agagaaaacc agagagaatg tgcccaacag cgactcccag
     1501 cctcctcctt cctgtttgac aacagcagtg ccaagtccct ccaaaccccg cacagcacaa
     1561 ttggtcctac agcgaatgca gcagttcaag agagcagacc ccgagcgttt gagacacgct
     1621 tcagaagagt gctccctcga ggctgcgcgg gaagaaaatg tcccaaagga tcctcaagag
     1681 gagatgatgg cggggaatgt gtatgggctt gggccccctg ccccagagag cgacgctgcg
     1741 gtggccttga ccctgcagca ggagtttgca cgggtaggag catcggcaca tgatgatagc
     1801 ctggaggaaa agggtttgtt cttctgccag atttgtcaaa agaacctctc agccatgaac
     1861 gtgacccgaa gggaacagca tgtgaacagg tgcttggatg aagctgaaaa gacactaaga
     1921 ccttctgtgc ctcagatccc tgagtgcccg atttgtggga aaccgtttct taccttaaag
     1981 agcagaacca gtcacttgaa gcagtgtgct gtgaagatgg aggttggccc ccagctcctg
     2041 cttcaggctg tgcggctgca gacagcacag cctgagggta gcagcagccc acccatgttc
     2101 agcttcagtg atcacagtag aggtctgaaa cggagaggac ccaccagcaa gaaggagcca
     2161 cggaagaggc ggaaggtgga cgaggcaccg tccgaggacc tgctggtggc catggctctg
     2221 tcccggtcgg agatggagcc gggtgcggct gtaccagcgc tcaggctgga aagtgccttt
     2281 tctgagagga taagaccaga agcagagaat aaaagtcgca agaagaaacc cccggtatcc
     2341 cccccattgt tgttagtcca ggactctgaa accacaggcc gacagataga ggaccgtgtg
     2401 gccctgctcc tctctgagga agtggaattg tctagcacgc caccacttcc tgccagcagg
     2461 attttaaagg aagggtggga aagagcgggc cagtgtcctc ctccacctga acgcaagcag
     2521 agctttctgt gggagggcag cgcactgact ggggcctggg ccatggagga cttctacacg
     2581 gccaggctgg tccctcctct cgtgccccag cggcctgccc agggccttat gcaggagccc
     2641 gtgccgcctc tggtgccacc tgagcactca gagctgagcg agcgaaggtc acccgctctc
     2701 cacggcaccc ccactgcagg ctgtggctcc aggggcccgt cgccttcggc cagccagagg
     2761 gagcaccagg ccctgcagga cctcgtggac ctggcgaggg agggactgag cgccagcccg
     2821 tggcccggca gtgggggcct ggctggctcg gaagggactg cagggttgga cgtggtgccc
     2881 ggcggccttc ctctgactgg gtttgtggtg ccatcgcagg acaagcaccc ggacaggggc
     2941 ggccgcacct tgctctccct cgggctgctg gttgctgact ttggcgccat ggtcaataac
     3001 ccacacctga gtgatgtcca gtttcagacg gacagcgggg aggtgcttta cgcccacaag
     3061 ttcgtgcttt atgcccgatg cccgctcctc atccagtatg tgaacaatga aggcttctcc
     3121 gctgtagagg acggggttct gacccagcgt gtcctgctgg gtgacgtgag caccgaggcc
     3181 gcccgcacgt tcctgcacta tctctacact gcggacactg gccttcctcc tggccttagc
     3241 tctgagctga gctccctggc ccacaggttt ggcgtgagtg agctcgttca cctgtgcgaa
     3301 caggtgccta ttgccactga ctcagagggc aaaccatggg aggagaagga agcagagaat
     3361 tgcgaaagca gggccgagaa tttccaggaa ctcttgaggt caatgtgggc agatgaagag
     3421 gaggaagcgg agactttgtt gaaatccaag gaccacgaag aagatcaaga aaacgtgaat
     3481 gaagcagaaa tggaagaaat ttatgaattt gcagctactc agcgaaagct tctccaggaa
     3541 gaaagggcag cgggtgccgg cgaggacgct gactggctgg agggtggcag tccggtttct
     3601 gggcaactcc tagcaggtgt ccaggtgcag aaacagtggg acaaggtgga ggagatggag
     3661 ccgttggagc caggaagaga tgaggccgcc accacctggg agaagatggg acagtgcgct
     3721 ctcccgccac cccagggcca gcactcaggg gcacggggag cagaggcccc tgagcaggag
     3781 gcgccagagg aggcgcttgg ccattccagc tgctccagcc cttccaggga ctgccaggca
     3841 gagagaaaag aaggctctct tccgcactca gatgatgccg gggattacga acagctcttc
     3901 tcatcaactc agggagagat ctcagagccg tcccaaataa caagtgagcc cgaggaacaa
     3961 agtggcgctg tcagggaaag ggggctggag gtttctcatc gcctggctcc ctggcaggca
     4021 tctccaccgc acccgtgccg cttcctattg gggcctcccc agggcgggag tccccgcggg
     4081 tctcatcaca caagtgggtc gtccctgtca acaccccggt cccgtggcgg aacttcccag
     4141 gtgggctccc caaccttgct gtctccagct gtgccatcaa agcagaaaag ggacaggagc
     4201 atcctcacgc tgtctaaaga gccagggcac cagaaaggca aagagcgtcg gtccgtgctg
     4261 gagtgcagaa ataagggggt cctgatgttc ccagaaaaat ctccgtctat tgacctaacc
     4321 cagtcaaatc ctgaccattc gagctccaga tctcagaaat cttcatccaa actgaacgaa
     4381 gaagatgagg tcatcctctt actggactcg gatgaggagc tggagctaga acaaaccaaa
     4441 atgaagtcca tttctagtga tcctctggaa gaaaagaaag ctctagaaat tagccctagg
     4501 tcctgtgagc tgttttccat cattgatgtt gatgcagatc aggaaccttc ccagagccca
     4561 ccaagaagcg aagctgtgct gcagcaggag gatgaggggg cgctgccgga gaatcggggc
     4621 tctttgggca ggagaggggc tccctggctg ttctgtgacc gtgagagcag ccccagcgag
     4681 gccagcacca cagacacctc gtggctggtg cccgccaccc cgctggccag cagaagccgt
     4741 gactgttctt cccagaccca aatcagcagc ctcaggagcg ggctggccgt gcaggcggtg
     4801 actcagcaca cgcccagggc ctcagtagga aacagggaag ggaacgaagt cgcacagaag
     4861 ttttctgtca tcaggcccca gacaccaccg ccccagacac cgtcctcatg cctcactccc
     4921 gtctctccag gaacttctga cggcagaagg caaggccaca gaagcccttc ccgtccccac
     4981 cccgggggcc acccgcactc ctctccgctg gctccacatc ccatctcagg ggaccgcgcc
     5041 cacttcagca ggcggttcct gaaacactcg ccgcctgggc caagcttcct gaaccagacc
     5101 ccagcgggtg aagtggtgga agtcggagac agtgacgatg agcaggaggt ggcctcccat
     5161 caggccaaca gaagcccccc actggacagt gaccccccaa ttccaattga cgactgctgc
     5221 tggcacatgg agcccctctc gccaattccc attgaccact ggaacctgga gcggaccggc
     5281 cccctgagca ccagcagccc cagccgcagg atgaacgagg ccgccgacag ccgtgactgt
     5341 cgctccccgg gactcctgga caccaccccc atccgaggaa gctgcactac ccagaggaaa
     5401 ttgcaagaga agtcctcggg cgcgggctcc ctggggaaca gcaggccgag ctttctgaat
     5461 tcggctctgt gggacgtttg ggacggggaa gagcagaggc ctccagagac ccctcctccg
     5521 gcccagatgc caagcgctgg tggagctcag aagcccgaag ggttagagac acccaaaggt
     5581 gctaatcgga agaagaactt gccccccaaa gtgcccataa cgccgatgcc acagtattcc
     5641 attatggaga cgccggtgct gaagaaggaa ctggataggt ttggagtccg ccctctgcct
     5701 aaacgccaga tggttctgaa gctgaaggag atattccagt acactcacca gaccctggac
     5761 tcagactccg aggacgagag ccagtcctca cagccgctgt tgcaggcgcc tcactgccag
     5821 accctcgcct cccagaccta caagccttca agggcagggg tccatgccca gcaggaggcc
     5881 accacaggac ctggggccca taggcccaag ggacctgcta agaccaaggg cccccgacat
     5941 caaaggaagc atcatgaaag catcacaccc ccaagcaggt cgcccaccaa ggaggcacct
     6001 ccaggcctca atgatgacgc ccagatccca gcctctcaag aatccgtggc cacctctgtg
     6061 gatggcagtg acagctcctt gagctcacag agttcttcct cctgtgagtt tggagcggca
     6121 tttgagtctg caggtgaaga ggagggcgag ggggaggtca gtgcctcgca ggcagccgtg
     6181 caggcggcgg acacagacga ggcgctgagg tgctacatcc gctccaagcc ggccctgtac
     6241 cagaaggtgc tgctgtacca gccctttgag ctgcgggagc tgcaggcaga gctgaggcag
     6301 aacggcctcc gtgtgtcctc gcgcaggctg ttggacttcc tggacaccca ctgtatcacc
     6361 ttcaccactg ccgccacccg cagggagaag ctccagggca ggaggcggca gcctcggggc
     6421 aagaagaagg tggagcggaa ctgacaccca gctttcttgt acaaagtggc tcgagcaggg
     6481 atccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     6541 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     6601 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     6661 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcgt aggaattcgc cagcacagtg
     6721 gtcgaccctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     6781 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6841 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6901 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6961 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7021 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttaccatgac
     7081 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     7141 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     7201 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     7261 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     7321 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     7381 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     7441 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     7501 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     7561 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     7621 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     7681 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgataaaata
     7741 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     7801 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga
     7861 gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc
     7921 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg
     7981 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     8041 tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
     8101 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
     8161 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
     8221 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct
     8281 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
     8341 actacccgtc agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt
     8401 tcttaagtat ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga
     8461 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8521 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8581 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8641 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8701 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8761 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8821 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8881 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8941 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9001 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9061 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9121 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9181 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9241 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9301 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9361 ttttgcggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9421 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9481 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9541 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9601 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9661 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9721 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9781 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9841 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9901 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9961 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10021 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10081 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10141 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10201 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10261 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10321 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10381 acatttcccc gaaaagtgcc acctgcagcc tgaatatggg ccaaacagga tatctgtggt
    10441 aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa
    10501 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
    10561 gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
    10621 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
    10681 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc
    10741 cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
    10801 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
    10861 cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga
    10921 ccaccgaccc accaccggga ggcaagctgg ccagcaactt atctgtgtct gtccgattgt
    10981 ctagtgtcta tgactgattt tatgcgcctg cgtcggtact agttagctaa ctagctctgt
    11041 atctggcgga cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga
    11101 cgtcccaggg actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg
    11161 aatccgaccc cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg
    11221 cctccgtctg aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag
    11281 cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
    11341 tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag
    11401 cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
    11461 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
    11521 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
    11581 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
    11641 gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct
    11701 cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc
    11761 ggccggatcc cagtgtggtg gtactaatag taatcaatta cggggtcatt agttca
//
