LOCUS       sequence-494907-        8408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of OsTIR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Hubbe P, Sharma C, Pajonk O, Peters N, Guschtschin-Schmidt N, 
            Friemel N, Schuck S
  TITLE     An optimised system for rapid auxin-inducible protein degradation in
            budding yeast
  JOURNAL   bioRxiv 2025.09.24.678194
REFERENCE   2  (bases 1 to 8408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.24.678194"
COMMENT     Sequence Label: pRS303K-Padh-OsTIR1(F74G)
FEATURES             Location/Qualifiers
     source          1..8408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..47)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     147..166
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        313..500
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     gene            complement(771..2127)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(771..968)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(974..1783)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1697..1716
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1784..2127)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        2206..2910
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             2962..4689
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein, 
                     mutated for use in the AID2 system of inducible degradation
                     (Yesbolatova et al., 2020)"
                     /label=OsTIR1(F74G)
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHGADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     terminator      4696..4943
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4712..4730)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(5468..5923)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5605..5626)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5817..5836
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(5900..6187)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSSKLTLTKGNKSWYRAPPSNSPYSESYYNSLAVVLQRRD
                     WENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     6049..6071
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6063..6080
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6064..6080
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6087..6106
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6087..6105
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6144..6162)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6183..6199)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6183..6199)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6196..6218)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6207..6223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6231..6237,6238..6255,6256..6261))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6276..6297
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6414..6431)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6585..7173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6665..6684)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7344..8135,8136..8204))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7967..7986
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8205..8309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8377..8395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
       61 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      121 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
      181 taaattcccg ttttaagagc ttggtgagcg ctaggagtca ctgccaggta tcgtttgaac
      241 acggcattag tcagggaagt cataacacag tcctttcccg caattttctt tttctattac
      301 tcttggcctc ctctagtaca ctctatattt ttttatgcct cggtaatgat tttcattttt
      361 ttttttccac ctagcggatg actctttttt tttcttagcg attggcatta tcacataatg
      421 aattatacat tatataaagt aatgtgattt cttcgaagaa tatactaaaa aatgagcagg
      481 caagataaac gaaggcaaag atgacagagc agaaagccct agtaaagcgt attacaaatg
      541 aaaccaagat tcagattgcg atctctttaa agggtggtcc cctagcgata gagcactcga
      601 tcttcccaga aaaagaggca gaagcagtag cagaacaggc cacacaatcg caagtgatta
      661 acgtccacac aggtataggg tttctggacc atatgataca tgctctggat catcgatgaa
      721 ttcgagctcg ttttcgacac tggatggcgg cgttagtatc gaatcgacag cagtatagcg
      781 accagcattc acatacgatt gacgcatgat attactttct gcgcacttaa cttcgcatct
      841 gggcagatga tgtcgaggcg aaaaaaaata taaatcacgc taacatttga ttaaaataga
      901 acaactacaa tataaaaaaa ctatacaaat gacaagttct tgaaaacaag aatcttttta
      961 ttgtcagtac tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1021 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1081 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1141 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1201 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     1261 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1321 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1381 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1441 ctgaatcagg atattcttct aatacctgga atgctgtttt gccggggatc gcagtggtga
     1501 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1561 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1621 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1681 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1741 aatttaatcg cggcctcgaa acgtgagtct tttccttacc catggttgtt tatgttcgga
     1801 tgtgatgtga gaactgtatc ctagcaagat tttaaaagga agtatatgaa agaagaacct
     1861 cagtggcaaa tcctaacctt ttatatttct ctacaggggc gcggcgtggg gacaattcaa
     1921 cgcgtctgtg aggggagcgt ttccctgctc gcaggtctgc agcgaggagc cgtaattttt
     1981 gcttcgcgcc gtgcggccat caaaatgtat ggatgcaaat gattatacat ggggatgtat
     2041 gggctaaatg tacgggcgac agtcacatca tgcccctgag ctgcgcacgt caagactgtc
     2101 aaggagggta ttctgggcct ccatgtcgct ggccgggtga cccggcgggg acgaggcaag
     2161 ctaaacagat ctggcgcgcc ttaattaacc cggggatccg agctcatcct tttgttgttt
     2221 ccgggtgtac aatatggact tcctcttttc tggcaaccaa acccatacat cgggattcct
     2281 ataatacctt cgttggtctc cctaacatgt aggtggcgga ggggagatat acaatagaac
     2341 agataccaga caagacataa tgggctaaac aagactacac caattacact gcctcattga
     2401 tggtggtaca taacgaacta atactgtagc cctagacttg atagccacca tcatatcgaa
     2461 gtttcactac cctttttcca tttgccatct attgaagtaa taataggcgc atgcaacttc
     2521 ttttcttttt ttttcttttc tctctccccc gttgttgtct caccatatcc gcaatgacaa
     2581 aaaaatgatg gaagacacta aaggaaaaaa ttaacgacaa agacagcacc aacagatgtc
     2641 gttgttccag agctgatgag gggtatctcg aagcacacga aactttttcc ttccttcatt
     2701 cacgcacact actctctaat gagcaacggt atacggcctt ccttccagtt acttgaattt
     2761 gaaataaaaa aaagtttgct gtcttgctat caagtataaa tagacctgca attattaatc
     2821 ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt gtttcttttt ctgcacaata
     2881 tttcaagcta taccaagcat acaatcaact ccaagctggc cgctctagaa gaacttagtt
     2941 tcgacggatt ctagaactag tatgacttat tttcctgaag aagttgtaga acacatattt
     3001 tcctttttac ctgctcaaag agatagaaac accgtctcct tggtctgtaa ggtttggtac
     3061 gaaatcgaaa gattgtctag aagaggtgtt tttgttggta attgttacgc agttagagct
     3121 ggtagagttg ctgcaagatt cccaaacgtt agagcattga ctgttaaagg taaacctcat
     3181 ggtgctgatt tcaatttggt tccacctgat tggggtggtt atgctggtcc atggattgaa
     3241 gctgcagcta gaggttgtca cggtttggaa gaattgagaa tgaagagaat ggttgtttct
     3301 gatgaatcat tggaattgtt agcaagatct tttcctagat tcagagcttt ggttttgatc
     3361 tcatgtgaag gtttttccac cgatggttta gcagctgttg cttcacattg taagttgttg
     3421 agagaattgg atttgcaaga aaacgaagtt gaagatagag gtccaagatg gttatcttgt
     3481 tttcctgatt cctgtacaag tttggtttct ttgaacttcg catgtatcaa gggtgaagtt
     3541 aacgctggta gtttggaaag attagtttct agatcaccaa atttgagatc tttgagattg
     3601 aacagatccg ttagtgttga taccttggca aagatcttgt tgagaactcc taatttggaa
     3661 gatttgggta ctggtaactt gacagatgat ttccaaacag aatcatactt caagttgacc
     3721 tccgctttgg aaaagtgtaa gatgttgaga tctttgtcag gtttttggga tgcaagtcca
     3781 gtttgtttgt cttttatata tcctttgtgt gctcaattga ctggtttgaa tttgtcttac
     3841 gcaccaactt tggatgctag tgatttgaca aagatgatct ctagatgtgt taagttgcaa
     3901 agattgtggg ttttggattg tatctcagat aaaggtttgc aagttgttgc ttcttcttgt
     3961 aaggatttgc aagaattgag agtttttcca agtgatttct atgttgcagg ttactctgct
     4021 gttacagaag aaggtttagt tgcagtttca ttgggttgtc ctaagttgaa ctccttgttg
     4081 tacttctgtc atcaaatgac caacgcagct ttggttactg ttgctaaaaa ttgtccaaac
     4141 ttcactagat tcagattgtg tatcttggaa ccaggtaaac ctgatgttgt tacatcacaa
     4201 cctttagatg aaggttttgg tgcaatcgtt agagagtgta agggtttgca aagattgtcc
     4261 atcagtggtt tgttgacaga taaggttttt atgtacatcg gtaaatacgc taagcaattg
     4321 gaaatgttgt caatcgcatt cgctggtgac tccgataagg gtatgatgca tgttatgaac
     4381 ggttgtaaga acttaagaaa gttggaaatc agagattctc catttggtga cgcagctttg
     4441 ttgggtaatt tcgcaagata tgaaacaatg agatctttgt ggatgtccag ttgtaacgtt
     4501 accttgaaag gttgtcaagt tttagcttca aagatgccaa tgttgaacgt tgaagttatt
     4561 aacgaaagag atggttccaa tgaaatggaa gaaaaccatg gtgacttgcc taaagttgaa
     4621 aagttatacg tctacagaac aaccgcaggt gccagagatg atgctcctaa cttcgtcaag
     4681 attttatgac tcgagtcatg taattagtta tgtcacgctt acattcacgc cctcccccca
     4741 catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt
     4801 tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc
     4861 tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg
     4921 gacgctcgaa ggctttaatt tgcggccggt cgacctgcag cgtacgcagt tgtcgaactt
     4981 ggtttgcaaa gggagaaagt aggagatctc tcttgcgaga tgatcccgca ttttcttgaa
     5041 agctttgcag aggctagcag aattaccctc cacgttgatt gtctgcgagg caagaatgat
     5101 catcaccgta gtgagagtgc gttcaaggct cttgcggttg ccataagaga agccacctcg
     5161 cccaatggta ccaacgatgt tccctccacc aaaggtgttc ttatgtagtg acaccgatta
     5221 tttaaagctg cagcatacga tatatataca tgtgtatata tgtataccta tgaatgtcag
     5281 taagtatgta tacgaacagt atgatactga agatgacaag gtaatgcatc attctatacg
     5341 tgtcattctg aacgaggcgc gctttccttt tttctttttg ctttttcttt ttttttctct
     5401 tgaactcgac ggatctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     5461 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     5521 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     5581 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     5641 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     5701 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     5761 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     5821 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     5881 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc
     5941 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     6001 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     6061 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttggaggggg
     6121 gggcccggta ccagcttttg ttccctttag tgagggttaa tttcgagctt ggcgtaatca
     6181 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6241 gccggaagca taaagtgtaa agcctggggt gcccaatgag tgagctaact cacattaatt
     6301 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6361 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6421 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6481 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6541 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6601 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6661 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6721 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6781 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6841 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6901 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6961 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7021 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7081 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7141 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7201 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7261 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7321 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7381 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7441 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7501 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7561 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7621 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7681 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7741 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7801 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7861 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7921 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7981 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8041 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8101 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8161 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8221 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8281 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     8341 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     8401 cgtctcgc
//
