LOCUS       sequence-494908-        8384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of OsTIR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Hubbe P, Sharma C, Pajonk O, Peters N, Guschtschin-Schmidt N, 
            Friemel N, Schuck S
  TITLE     An optimised system for rapid auxin-inducible protein degradation in
            budding yeast
  JOURNAL   bioRxiv 2025.09.24.678194
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.24.678194"
COMMENT     Sequence Label: pRS303K-Prnr1-OsTIR1(F74G)
FEATURES             Location/Qualifiers
     source          1..8384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..47)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     147..166
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        313..500
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     gene            complement(771..2127)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(771..968)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(974..1783)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1697..1716
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1784..2127)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             2938..4665
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein, 
                     mutated for use in the AID2 system of inducible degradation
                     (Yesbolatova et al., 2020)"
                     /label=OsTIR1(F74G)
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHGADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     terminator      4672..4919
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4688..4706)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(5444..5899)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5581..5602)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5793..5812
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(5876..6163)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSSKLTLTKGNKSWYRAPPSNSPYSESYYNSLAVVLQRRD
                     WENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     6025..6047
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6039..6056
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6040..6056
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6063..6082
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6063..6081
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6120..6138)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6159..6175)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6159..6175)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6172..6194)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6183..6199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6207..6213,6214..6231,6232..6237))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6252..6273
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6390..6407)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6561..7149)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6641..6660)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7320..8111,8112..8180))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7943..7962
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8181..8285)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8353..8371
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
       61 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      121 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
      181 taaattcccg ttttaagagc ttggtgagcg ctaggagtca ctgccaggta tcgtttgaac
      241 acggcattag tcagggaagt cataacacag tcctttcccg caattttctt tttctattac
      301 tcttggcctc ctctagtaca ctctatattt tttatgcctc ggtaatgatt ttcatttttt
      361 ttttttccac ctagcggatg actctttttt tttcttagcg attggcatta tcacataatg
      421 aattatacat tatataaagt aatgtgattt cttcgaagaa tatactaaaa aatgagcagg
      481 caagataaac gaaggcaaag atgacagagc agaaagccct agtaaagcgt attacaaatg
      541 aaaccaagat tcagattgcg atctctttaa agggtggtcc cctagcgata gagcactcga
      601 tcttcccaga aaaagaggca gaggcagtag cagaacaggc cacacaatcg caagtgatta
      661 acgtccacac aggtataggg tttctggacc atatgataca tgctctggat catcgatgaa
      721 ttcgagctcg ttttcgacac tggatggcgg cgttagtatc gaatcgacag cagtatagcg
      781 accagcattc acatacgatt gacgcatgat attactttct gcgcacttaa cttcgcatct
      841 gggcagatga tgtcgaggcg aaaaaaaata taaatcacgc taacatttga ttaaaataga
      901 acaactacaa tataaaaaaa ctatacaaat gacaagttct tgaaaacaag aatcttttta
      961 ttgtcagtac tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1021 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1081 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1141 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1201 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     1261 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1321 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1381 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1441 ctgaatcagg atattcttct aatacctgga atgctgtttt gccggggatc gcagtggtga
     1501 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1561 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1621 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1681 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1741 aatttaatcg cggcctcgaa acgtgagtct tttccttacc catggttgtt tatgttcgga
     1801 tgtgatgtga gaactgtatc ctagcaagat tttaaaagga agtatatgaa agaagaacct
     1861 cagtggcaaa tcctaacctt ttatatttct ctacaggggc gcggcgtggg gacaattcaa
     1921 cgcgtctgtg aggggagcgt ttccctgctc gcaggtctgc agcgaggagc cgtaattttt
     1981 gcttcgcgcc gtgcggccat caaaatgtat ggatgcaaat gattatacat ggggatgtat
     2041 gggctaaatg tacgggcgac agtcacatca tgcccctgag ctgcgcacgt caagactgtc
     2101 aaggagggta ttctgggcct ccatgtcgct ggccgggtga cccggcgggg acgaggcaag
     2161 ctaaacagat ctggcgcgcc ttaattaacc cggggatccg agctcggata tcgtaaacaa
     2221 aggcgttacc atagaaatgt actgattggc agaattactc ttcaggagaa tctttcatac
     2281 aaaggtattc cattggggaa aatctcgtta ccaagtcaat gctgaacttt ctatggcctt
     2341 tgtttactat cgttaattat tttacgacca cttctgggta gaaatatttc gtagccctgg
     2401 aacgagcttg tttacgcgtt ttatcccatt atatggcacc caaatcaaat ttaaaaagaa
     2461 aaaacgcgta aacagtgtcg ggtaagttca tcctctgtta ctttaattgc ttcttttttt
     2521 gaaattctaa gtaaacgcgt cattttgatc ctcaggacac agaaatcctt gcagaatctt
     2581 attgggtgtt gaatagagga cgcgtaaaaa cgatatggaa atttttttca tatagtgtag
     2641 aaagaatagg ttggcgtagg tagtttcgtg tttgatagaa acctccaaca aagtctgcaa
     2701 ctcacgtttt agaataacaa gtttagagtt tatcttgttg cctttgttaa gtcagtacca
     2761 ttgaataaaa attatataaa ggagctaata tttcattgtt ggaaaattac tctaccataa
     2821 ttgaagcata tctcatcctt ttcatccttt tcaacgcaag agagacacca acgaacaaca
     2881 ctttatttgt tgatatatta acatcagaac ttagtttcga cggattctag aactagtatg
     2941 acttattttc ctgaagaagt tgtagaacac atattttcct ttttacctgc tcaaagagat
     3001 agaaacaccg tctccttggt ctgtaaggtt tggtacgaaa tcgaaagatt gtctagaaga
     3061 ggtgtttttg ttggtaattg ttacgcagtt agagctggta gagttgctgc aagattccca
     3121 aacgttagag cattgactgt taaaggtaaa cctcatggtg ctgatttcaa tttggttcca
     3181 cctgattggg gtggttatgc tggtccatgg attgaagctg cagctagagg ttgtcacggt
     3241 ttggaagaat tgagaatgaa gagaatggtt gtttctgatg aatcattgga attgttagca
     3301 agatcttttc ctagattcag agctttggtt ttgatctcat gtgaaggttt ttccaccgat
     3361 ggtttagcag ctgttgcttc acattgtaag ttgttgagag aattggattt gcaagaaaac
     3421 gaagttgaag atagaggtcc aagatggtta tcttgttttc ctgattcctg tacaagtttg
     3481 gtttctttga acttcgcatg tatcaagggt gaagttaacg ctggtagttt ggaaagatta
     3541 gtttctagat caccgaattt gagatctttg agattgaaca gatccgttag tgttgatacc
     3601 ttggcaaaga tcttgttgag aactcctaat ttggaagatt tgggtactgg taacttgaca
     3661 gatgatttcc aaacagaatc atacttcaag ttgacctccg ctttggaaaa gtgtaagatg
     3721 ttgagatctt tgtcaggttt ttgggatgca agtccagttt gtttgtcttt tatatatcct
     3781 ttgtgtgctc aattgactgg tttgaatttg tcttacgcac caactttgga tgctagtgat
     3841 ttgacaaaga tgatctctag atgtgttaag ttgcaaagat tgtgggtttt ggattgtatc
     3901 tcagataaag gtttgcaagt tgttgcttct tcttgtaagg atttgcaaga attgagagtt
     3961 tttccaagtg atttctatgt tgcaggttac tctgctgtta cagaagaagg tttagttgca
     4021 gtttcattgg gttgtcctaa gttgaactcc ttgttgtact tctgtcatca aatgaccaac
     4081 gcagctttgg ttactgttgc taaaaattgt ccaaacttca ctagattcag attgtgtatc
     4141 ttggaaccag gtaaacctga tgttgttaca tcacaacctt tagatgaagg ttttggtgca
     4201 atcgttagag agtgtaaggg tttgcaaaga ttgtccatca gtggtttgtt gacagataag
     4261 gtttttatgt acatcggtaa atacgctaag caattggaaa tgttgtcaat cgcattcgct
     4321 ggtgactccg ataagggtat gatgcatgtt atgaacggtt gtaagaactt aagaaagttg
     4381 gaaatcaggg attctccatt tggtgacgca gctttgttgg gtaatttcgc aagatatgaa
     4441 acaatgagat ctttgtggat gtccagttgt aacgttacct tgaaaggttg tcaagtttta
     4501 gcttcaaaga tgccaatgtt gaacgttgaa gttattaacg aaagagatgg ttccaatgaa
     4561 atggaagaaa accatggtga cttgcctaaa gttgaaaagt tatacgtcta cagaacaacc
     4621 gcaggtgcca gagatgatgc tcctaacttc gtcaagattt tatgactcga gtcatgtaat
     4681 tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag
     4741 gagttagaca acctggagtc taggtcccta tttatttttt tatagttatg ttagtattaa
     4801 gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta
     4861 acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg
     4921 gccggtcgac ctgcagcgta cgcagttgtc gaacttggtt tgcaaaggga gaaagtagga
     4981 gatctctctt gcgagatgat cccgcatttt cttgaaagct ttgcagaggc tagcagaatt
     5041 accctccacg ttgattgtct gcgaggcaag aatgatcatc accgtagtga gagtgcgttc
     5101 aaggctcttg cggttgccat aagagaagcc acctcgccca atggtaccaa cgatgttccc
     5161 tccaccaaag gtgttcttat gtagtgacac cgattattta aagctgcagc atacgatata
     5221 tatacatgtg tatatatgta tacctatgaa tgtcagtaag tatgtatacg aacagtatga
     5281 tactgaagat gacaaggtaa tgcatcattc tatacgtgtc attctgaacg aggcgcgctt
     5341 tccttttttc tttttgcttt ttcttttttt ttctcttgaa ctcgacggat ctatgcggtg
     5401 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat
     5461 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     5521 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     5581 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     5641 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     5701 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     5761 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     5821 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     5881 gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     5941 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     6001 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     6061 tgtaatacga ctcactatag ggcgaattgg aggggggggc ccggtaccag cttttgttcc
     6121 ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6181 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6241 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6301 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6361 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6421 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6481 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6541 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6601 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6661 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6721 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gcatctcagt
     6781 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6841 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6901 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6961 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7021 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaagcaa
     7081 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7141 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7201 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7261 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7321 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7381 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7441 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7501 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7561 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7621 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7681 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7741 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7801 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7861 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7921 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7981 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8041 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8101 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8161 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8221 tattgtctca tgagcggata catatttgaa tgtatttaga aaaatagaca aataggggtt
     8281 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     8341 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgc
//
