LOCUS       sequence-49499-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  TITLE     Kuwabara unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPK719
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..62
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     46..62
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(249..260)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gactcactat agggcgaatt ggagctccac cgcggtggcg gccgctctag aactagtgga
       61 tcccccgggc tgcaggaatt cgataagatc tatgcttgcg ctttgagcca atttatccaa
      121 gtccttgtaa aaagtattcg aaaattgtta acggataaaa tgtttattat aatatcaaaa
      181 acaatttgtc agttgaccac tttttgatat atttgacaga aaccgggatg aattggctca
      241 aagttagggc gcccttctat tacagggttt ctgataacaa accggttatt aactcccaac
      301 aagggatgat ttcaattcat catgctcaaa ttgacccaaa ttaagttaca tgacaaattc
      361 atcgcccttt tcaactcttc ttggctcatc atctgttatt ctgttcctat tctgtctgca
      421 ccctataccc ctttgcatac tctctcgtca ttcctttctt ggatagtgtc ttcttctccc
      481 cagctctgct acttctatga cttgcgcccg ctgcttttcc gctcgttcct ctcctctctg
      541 acgtcttctt ctgcttcttt tctccttcct ttttcccttc tcaatcttcc tctttttccc
      601 atttctgtca atcattcgaa gaagaagaag aagaagaccc tcattcattt catttttttt
      661 tctgtcggtg tgtgctgctc ggttaagagt gagctctcta ttccacgtct tcttcttttt
      721 cttcttcgat tcgaatcaat cactccacca aaacgcattc gtttttggga ttcaccccgc
      781 ggttcgcaac aggtttcttt ttcaaattat tagcgttata aatagaaaaa tg
//
