LOCUS       sequence-49500-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Tan MJ, White EA, Sowa ME, Harper JW, Aster JC, Howley PM
  TITLE     Cutaneous beta-human papillomavirus E6 proteins bind Mastermind-like
            coactivators and repress Notch signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Apr 30.
  PUBMED    22547818
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Apr 30."
COMMENT     Sequence Label: p6875 NTap-FLAG-HA-BPV1 E6
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..54
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             92..115
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             128..154
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    170..194
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(606..630)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
       61 tctaggcgcc ggaattagat ctgccaccat ggactacaag gatgacgatg acaagctcga
      121 tggaggatac ccatacgatg ttccagatta cgctgctcga ggttatcaaa caagtttgta
      181 caaaaaagtt ggcgacctga aaccttttgc aagaaccaat ccattctcag ggttggattg
      241 tctgtggtgc agagagcctc ttacagaagt tgatgctttt aggtgcatgg tcaaagactt
      301 tcatgttgta attcgggaag gctgtagata tggtgcatgt accatttgtc ttgaaaactg
      361 tttagctact gaaagaagac tttggcaagg tgttccagta acaggtgagg aagctgaatt
      421 attgcatggc aaaacacttg ataggctttg cataagatgc tgctactgtg ggggcaaact
      481 aacaaaaaat gaaaaacatc ggcatgtgct ttttaatgag cctttctgca aaaccagagc
      541 taacataatt agaggacgct gctacgactg ctgcagacat ggttcaaggt ccaaataccc
      601 atagtaccca actttcttgt acaaagtggt tcgataacga attccgcccc ccccccctaa
      661 cgttactggc cgaagccgct tggaataagg ccggngtgcg tttgtctata tgttaatttc
      721 cacccatatt gccgtctttt tggcaatgtg aagggccccg gaaaacctgg gccctgtctt
      781 cttgacgag
//
