LOCUS       sequence-495012-       18800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of mouse ANK3 480 isoform with microexon E35a with N 
            terminal flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18800)
  TITLE     Yang lab plasmids 2025
REFERENCE   2  (bases 1 to 18800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAG480-GFP
FEATURES             Location/Qualifiers
     source          1..18800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6595..6621
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HLHHHHHHH"
     CDS             6601..6627
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHYH"
     CDS             6604..6630
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHYHH"
     RBS             6655..6663
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      7838..7847
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7844..7846,7847..7849,7850..8563)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7844..7846,7847..7849,7850..8560)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7889..7910)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8150..8169)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8497..8518
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8686..8807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8723..8742)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8777..8796
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8814..9269)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8951..8972)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9163..9182
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9296..9400
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9402..9759
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9402..9593
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9425..9445)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9610..9745
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9794..10588
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9794..10588
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9848..9867)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10458..10477
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10776..10795)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10820..10867
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11196..11784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11685..11704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        11959..12262
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12264..12540
                     /label=chicken beta-actin promoter
     intron          12541..13557
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     13481..13503
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     13565..13584
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 aattaacgga tgcaaagcca ctgccacatt acaggacaaa atttctacac ccacaaatgc
       61 tgtgagctcg gtggtgagcg cggcccctga cacggtggag aaagcgctct ccaccacaac
      121 agccatgccc ttttccccac tcaggtctta cgtttccgct gcagcgccct ctgcttttca
      181 gtcccttaga gctccctctg ccagtgcact ctacacctcc ctcgggcctt cagtagctgt
      241 tactacctca tccgtaactt catcgataat cacagtgcca gtatactcgg tagtcaatgt
      301 tttgccagaa ccagcattga agaaacttcc agactctaac tcgctcacaa aatcggcagc
      361 ggctttgctg tcgcccatta aaacattgac tacggagaca cgtcctcagc cccatttcaa
      421 tcgaacgtca tctcccgtca agtcctctct gttcctggca tcctcggccc ttaagccgtc
      481 agtaccatct tctttatctt ccagtcagga gatcttaaaa gatgtggccg agatgaagga
      541 ggacctcatg agaatgaccg ccatactgca gacggatgtg cctgaggaga agccattcca
      601 gaccgacctc ccccgagaag ggaggataga cgacgaagag cctttcaaaa tcgttgagaa
      661 agtgaaggaa gacttagtca aagtcagtga gatactcaaa aaggacgtgt gtgtcgagag
      721 caaagggcca cccaagtccc caaagagtga caaaggacac tctccagaag atgactggac
      781 ggaatttagt tctgaggaaa tacgagaagc caggcaggct gctgccagtc acgccccatc
      841 cctgcccgag agagtacatg gaaaggccaa cctcaccaga gtcatagact acctgaccaa
      901 tgacatcggg agcagttcac tgaccaactt aaagtacaag tttgaagagg ctaagaagga
      961 aggagaggag agacagaaga gaattttaaa gccagccatg gccttgcagg agcataaact
     1021 caaaatgccg ccagcctcca tgagaccttc cacctccgag aaggaactgt gcaagatggc
     1081 ggactccttt tttggaacag atgccatcct agagtctccc gatgactttt ctcaacatga
     1141 ccaagacaaa agtcccttgt ccgacagtgg ctttgaaacc cgaagtgaga aaacaccttc
     1201 agccccccaa agtgccgaga gcaccggccc taagccacta tttcatgaag tccccatccc
     1261 ccctgtcatc acagagacga gaactgaagt agtccacgtg atcaggagct acgagccctc
     1321 tactggggag atcccccagt cccagccaga ggaccccgtg tctcccaaac ctccccctac
     1381 atttatggag ttggaaccaa agcccaccgc ccttagcatt aaggaaaaag tgaaagcatt
     1441 ccaaatgaaa gccagtagcg aggaagagga ccacagtcgg gttttaagca aaggcatgcg
     1501 tgtcaaagaa gagactcaca tcaccacaac cacgaggatg gtgtatcact ccccgcctgg
     1561 cagtgagtgt gcctccgaga ggattgagga gaccatgtca gtccatgaca ttatgaaggc
     1621 ttttcagtcc gggcgagacc cttccaaaga gctggcgggg ctgtttgaac acaagtcggc
     1681 catgtctccg gatgttgcca agtctgctgc tgagacctcg gcccagcacg cagagaagga
     1741 caaccaaatg aaacccaaac tggagcgtat tatagaggtc cacatcgaaa aaggtaacca
     1801 agccgaaccc actgaagtca ttattagaga gaccaaaaag catccagaga aggaagtgtc
     1861 tgtgtatcag aaagacttat tatcccgggg aaatattaac ttaaaagatt ttctgccaga
     1921 gaagcacgat gctttccctt gccccgagga gcagggccag caagaagagg aggagtttgc
     1981 cgcagaagag tccttgcctt cttatctgga gtcttccaga gtaaacactc cagggtccca
     2041 agaagaagat agccgcccta gttccgcaca gcttctgtcc gatgactctt ataaaaccct
     2101 gaagcttctg agtcagcact ccgtagagta ccatgatgat gagctgtcag acctaagagg
     2161 ggagtcttac aggtttgccg agaaaatgct tctctcagaa aagctagatg tttctcactc
     2221 tgataccgag gaatcggtga cagaccatgc aggaccccct agctcagagt tacaggggtc
     2281 tgataagcga tccagagaaa aagtagccac tgccccccca aaagagatcc tctccaaaat
     2341 ctataaagat gtgtctgaaa atggtatagg gagagtgtct aaagatgagc attttgagaa
     2401 actgacagtg ttgcactacg cgggcaatgt cagtggtccc aaacatacca tgtggatgca
     2461 tttgtccgag gacagactag acagaggtag agagaagctg atgtatgaag atagggtgga
     2521 caggaccgtg aaggaggcag aagagaagtt gaccgaagtg tctcaatttt ttcgtgacaa
     2581 aaccgagaag ctcaacgacg aactgcagtc cccggagaaa aagccacgcc ccaaaaacgg
     2641 caaggaatac tcttctcaga gttccaccag cagtagcccc gagaaagtgt taacagaact
     2701 gttggcatcc aacgatgaat gggttaaggc caggcagcgc ggtccagatg gacaaggtgt
     2761 cccgcaggct gaggacagga aggcacccag tgggtccagc agccccgaga agagggtccc
     2821 ttcccaacag attgaggatg gccagcctcc agaggaagcc aaaagaactg tcattgctca
     2881 gagcagggga caagaaggac cccagtctgg atttcagctc aaacaatcta aactcagttc
     2941 cattagatta aaatttgaac aaggcgcacg ggccaaaagt aaggatcatg aggaaaaaaa
     3001 tctggacggg ccatcccgaa tccctgtaaa gaaaactcaa gaaaacaagc tgcccacgca
     3061 cccaggtttt gctagagaaa aacagcagaa ggctgtagat cccccagagg aaaaaatgtc
     3121 ggtgcaaaat gatgttctga ttttcagacc tgagcatggc aaaagcagtg agatggttgt
     3181 gagcgaatct ggctccagtg atggcaaaag ccacagaact gagatgttaa gtaaggcaac
     3241 gcctgacttc tttcctgagc aacaggttga agactcagcc tgtcccataa cctcagatct
     3301 agagaccaag ggaccatggg accgaaaggt ctttagaaca tgggaaagtt ctggggccaa
     3361 taacaataag gctcagaaag aacaactctc tcacgtgctc gttcacgaca tcagagagag
     3421 tcacgttggt caccctgaca gtagcgagaa cggtgaccca aagaatgatt ttatgtatgt
     3481 gactgagaga gaacacaaaa tgttaacgaa cggctctctc tcagaaatta aagaaatgag
     3541 tgtaaagtct ccctccaaga aagtgttata cagggaatac atggttaaag aaggggacct
     3601 tcccagcagc actctcgacc cccccaggag aagcgagagc tcggcagcat ctcacactcc
     3661 catcagggtc acagatgaga ggaggatgct gtcatcaaac attcctgatg ggatttgtga
     3721 gcagacaaca tttccaaaac aagacctgtc ccaaagagtg tcccggccaa gtgtgagcaa
     3781 aggggcagtt gagagccagc actttaactc catagacgat gagaaagtta cctattccga
     3841 gatgagcaaa gtttccaaac gtcagagtta cgtaggccta tgcccagctc tcgatgaaac
     3901 cgagacctct cccaccaaat ctcccgattc cttagagttt agcccaggga aggactctcc
     3961 ctctagcgat gtgttcgacc acggttcaat ggatgggtta gagaaggctg aaggaggtaa
     4021 agagatcaaa accttacctg tttatgtcag cttcgtgcaa gtggggaaac agtatgaaaa
     4081 ggaactccaa caaggaggtg tgaaaaaaat catcagtcag gagtgtaaga cagtgcagga
     4141 agccaggggg actttctata ccgctagaca gcagaagcag cctccttctc cccagggaag
     4201 tccagaagac gatactctag agcaagtgtc ctttctagac agctctggga agagcccttt
     4261 gaccccagaa actcccagtt ccgaggaagt gagttatgag ttcacgtcta agacgccaga
     4321 ctcactcata gcttttatac caggacagcc cagcccaatt cctgaggttt ctgaggagga
     4381 ggaggaggag gaagagccca aagcagcccc actaaggcag gtgcccgtgg agaaggaaac
     4441 agatcgtgat gtgagcaaag actctgtcca aagacccaaa tgcaaccgag tggcctatat
     4501 tgagtttcct cctcctcccc ccctggatgc cgaccagatg gagtcagata agaaacacga
     4561 gtatctcccc gacagggagg ttgacatgat ggaagtcagc cttcaggatg agtctgacaa
     4621 gtaccagttg gccgagccag tcatccgagt gcagccccca tcaccagtcc ctcctggtgc
     4681 cgatgccagt gagtccagcg atgacgagtc catgtatcaa ccggccccag ttaaaaaata
     4741 caccttcaag ctgaagggag tggacgaagg gcagaaagac acagcaaaat ccaaaacggc
     4801 caccacaaag gcttccaacc agaaagaggt ggagagtaac gggaaagaga gtgagtctgg
     4861 ccttgactca cctcagaatg aaaccgctca gaacggaaac aacgaccagt ctgtcaccga
     4921 gtgttccatc gccaccacgg ccgagttttc tcacgacacg gatgccacag agatagactc
     4981 tctggatggc tatgacctgc aggacgagga tgacggccta acagagagcg actctaaact
     5041 cccgagtcaa actatagaca ccaagaagga tgtctggacg gagggcattc tgaagccggc
     5101 cgaccgctct ttcagtcaga gcaaactgga agttattgaa gaggagggga aagtgggact
     5161 ggacgaagag aaaccattac cttctaaaag ctcgtcatct gaccgaaccc cagagaaggc
     5221 tgatcccaag tcgggggccc agtttttcac cctggaaggc agacacccgg acagatcagt
     5281 gttccctgat acttacttca gttacaaagt tgatgaagaa tttgccactc cctttaaaac
     5341 agtggcgacc aaaggtctag actttgaccc ttggcccaat aaccgagggg acaatgaagt
     5401 ttttgatggt aaatcccggg aagatgacac taagccattt ggtctggcgg tggacgaccg
     5461 ctctccggca accacccctg acacaacgcc agccagaaca ccaactgacg aaagtacccc
     5521 aactagtgag cctaacccct tcccatttca tgaaggaaaa atgtttgaga tgacccgcag
     5581 tggtgcaatt gacatgagca agagagattt tgtggaagag aggctccaat ttttccagat
     5641 tggtgagcat actcctgaag ggaagtcagg ggcccagggg gaagggggta cggtcactga
     5701 taccccacag ccagagtcag gggacacctc ggtagacacc aatctagaga gagatgtaga
     5761 ggcacctaca gtcgacccca accccagcat cccgaccaat ggagagtgtc aggaaggcac
     5821 agcctgcaat ggctgcctgg agaagtcggc agctgccact aacacctcaa aagttgaccc
     5881 caagtcacgc acacctataa aaatgggaat ttctgcatct accatgacca tgaagaaaga
     5941 aggctctgga gacacaacag ataagacaga ggctgtggtg accagttatc agggattaga
     6001 aaatgaaatc gtgaaagaga tctcaagcgc actcaatagc caggtgggca ttaggcccca
     6061 agagaaacac gattttcaaa aagataactt taataacaac aataatctgg atgcttccgc
     6121 tatgcagaca gataacacca caagtcacat agttctgaca ggacgcgctg catccacttg
     6181 taccacagag aaagctaatc cagtgaaagg ctcaggaaag agtccaggga ctcaaggacg
     6241 ctccagcaga gattctcaga aggaaccaat aggggttagg cggaaatcca agcttcccgt
     6301 aaaggccacc gcaccaaaag atgtcttccc gccaaaccat aagccagaca gtaaagcagg
     6361 taaactgagg caggttggtc aatatgagaa aaacaaagcc cttcctactt cttcatgtat
     6421 agattccaag tctcgaattc cagtcaaaaa cacacacagg gagaacctag tttcagtcag
     6481 aaaagcatgc gccacacaaa agcgagggca gccagagaaa ggcaaggcca aacagcctcc
     6541 atccaagttg ccagtaaagg taagatccac ctgtgtcacc gtcaccacca ccaacacctc
     6601 caccaccacc accaccacca ctaccaccac cgttaaagtt acagaaagtc agcgtaagga
     6661 ggtatgtaaa catcccattg aatattttaa gggaattagt ggcgagaccc taaaacttgt
     6721 ggaccgcctc acggaagaag acagaaagat gcaatcggag ttgtctgatg gggaggaaag
     6781 tacctcaagg agcacatcgc tgtccgagac ttcccggggc ggccagcctt cagttaccac
     6841 gaagtctgct agaaataaga gaacagaggc accgccctta aaatcaaaga gaggaaagag
     6901 ctgcagtagg aggactgttc cacagagtcc ttgtgagcgg acggatatca ggatggcaat
     6961 agtagccgat cacctgggac ttagttggac agagctggca agggaactga atttttcagt
     7021 ggatgaaatc aaccaaatac gagtggaaaa tcccaattct ttaatttctc agagcttcat
     7081 gttattaaaa aagtgggtga cgagagacgg gaagaatgcc acaactgatg ccttaacttc
     7141 ggtcttaaca aaaattaacc gaatagacat agtaactctg ctggaaggac caatatttga
     7201 ttatgggaat atttcaggca ccagaagctt tgcagatgaa aacaatgttt tccatgaccc
     7261 agttgatggt tggcagaacg agacgccaag tggaagccta gagtccccgg cgcaagcgcg
     7321 aagaataacc ggtgggttac tggaccgtct ggacgacagc tctgaccagg ttcgggatcc
     7381 cattacctca tacctcacag gagaagctgg gaagttcgaa gcaaacggaa accacgcaga
     7441 agtcattcca gaagcaaagg ccaaggccta ctttcctgaa tcccaaaacg atatagggaa
     7501 acagagcatc aaggagaact tgaaaccaaa aacacacgga tgtggtcgcg ctgaggaacc
     7561 agtgtcgccc ctcacagcct accagaaatc tctagaagaa accagcaagc ttgtcataga
     7621 agacgcacct aaaccctgtg tgcctgtcgg catgaaaaag atgaccagga ctccggctga
     7681 cggcaaagcc aggctcaacc tccaggaaga ggaggggtcc gccaggtcag agcctaagca
     7741 gggagaaggc tataaggtga aaacaaagaa ggaaatccgg aatgtggaga agaaagccca
     7801 ccagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga
     7861 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     7921 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     7981 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     8041 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     8101 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     8161 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     8221 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     8281 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     8341 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     8401 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     8461 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     8521 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag
     8581 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     8641 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     8701 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8761 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt
     8821 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     8881 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     8941 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     9001 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     9061 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     9121 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     9181 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     9241 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     9301 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     9361 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
     9421 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     9481 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     9541 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     9601 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9661 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9721 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     9781 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     9841 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     9901 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     9961 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
    10021 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
    10081 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
    10141 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
    10201 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
    10261 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
    10321 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
    10381 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
    10441 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
    10501 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
    10561 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
    10621 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
    10681 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    10741 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
    10801 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
    10861 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
    10921 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
    10981 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
    11041 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    11101 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    11161 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    11221 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    11281 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    11341 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    11401 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    11461 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    11521 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    11581 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    11641 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    11701 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    11761 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    11821 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    11881 cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag
    11941 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
    12001 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
    12061 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
    12121 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
    12181 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
    12241 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
    12301 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
    12361 gatgggggcg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
    12421 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
    12481 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
    12541 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
    12601 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
    12661 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
    12721 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
    12781 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
    12841 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
    12901 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
    12961 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
    13021 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
    13081 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
    13141 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
    13201 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
    13261 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
    13321 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
    13381 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
    13441 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
    13501 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
    13561 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt catggctcat
    13621 gccgcctccc agttaaagaa aaacagggat ttagaaatca atgcggaaga agagaccgag
    13681 aaaaaaaaga agcaccgtaa acggtcccgg gatcgcaaga aaaagtcgga tgccaatgca
    13741 agttacttga gagcagctcg ggcggggcac ctggaaaagg cccttgacta catcaaaaat
    13801 ggagtggacg tcaacatctg taaccagaat gggttgaatg cgctccatct tgcttccaaa
    13861 gaaggccatg tggaagtggt ctccgagctg ctgcagagag aagccaacgt cgatgcagcc
    13921 acaaagaaag gaaacacagc cttacacatc gcatccttgg ctggacaagc ggaagtggtc
    13981 aaggtcttgg ttacgaacgg agcgaacgtc aacgcacagt ctcagaatgg cttcacgccg
    14041 ctgtatatgg cggcccagga gaaccacctg gaagtcgtca ggtttcttct ggacaatggt
    14101 gccagccaaa gcctggctac agaggatggc ttcacgccat tggccgtggc tcttcaacaa
    14161 ggtcacgacc aagtcgtatc cctcctgctc gagaacgaca cgaagggaaa agtgcgcctc
    14221 ccagccctcc acatcgcggc ccggaaagat gacaccaaag cggcagctct gctcctacag
    14281 aacgacacca atgcagacat agagtcaaag atggtggtga atagagcaac tgagagtggc
    14341 ttcacctcac tccacatagc tgcccactac gggaatatca atgtggccac gttgctgtta
    14401 aaccgagcgg ctgccgtgga cttcaccgca cggaatgaca tcactccttt gcatgttgcc
    14461 tcgaaacggg gaaacgcaaa tatggtaaag ctgttgctcg accggggtgc gaagatcgac
    14521 gccaagacca gggacggtct gaccccgctg cactgcgggg cgagaagtgg ccacgagcag
    14581 gtggtagaga tgttgctgga cagagccgcc cccatccttt cgaaaaccaa gaatggactg
    14641 tccccactgc acatggccac acaaggagac catttaaact gcgtccaact cctcctccag
    14701 cacaacgtgc ccgtggatga cgtcaccaac gactacctga ccgccctcca cgtggcggcc
    14761 cactgcggcc attacaaagt tgccaaggtt cttttggata agaaagctaa ccccaatgcc
    14821 aaagccctga atggcttcac ccctctccat atcgcctgca aaaagaaccg aatccgagta
    14881 atggaactcc ttttgaagca cggtgcatct attcaagccg taaccgagtc gggccttacc
    14941 ccaatccatg tcgctgcctt catgggacat gtaaatatcg tgtcacagct aatgcatcat
    15001 ggagcctccc caaacaccac caacgtgaga ggagaaacag cattgcatat ggcggctcgg
    15061 tcggggcaag cagaagtggt gcggtatttg gtacaagatg gagctcaggt agaagcaaaa
    15121 gctaaggatg accagacccc actccacatt tcagcccgac tcgggaaggc tgacatagtg
    15181 caacaactgt tacagcaagg agcgtccccg aatgcagcaa caacttccgg gtacaccccc
    15241 cttcaccttt cggccagaga ggggcatgag gatgtagctg ccttccttct ggaccatgga
    15301 gcatctttat ccataacaac aaagaaggga ttcacgcctc tgcatgtggc tgcaaagtat
    15361 gggaagcttg aagtggcaag tctcctgctg cagaagagtg cgtctcctga tgccgctggg
    15421 aagagcgggc taactccact gcatgtagca gcacattacg ataatcagaa agtggccctt
    15481 ctgcttttgg accagggagc ctcaccccac gcagctgcaa agaacggtta tacgccactg
    15541 cacatcgccg ccaagaagaa ccagatggac atagccacgt ccctgctgga gtacggtgct
    15601 gatgcaaacg ccgttacccg gcaagggatt gcgtccgtcc accttgcagc gcaggaaggg
    15661 cacgtggaca tggtgtcgct gctcctcagt agaaatgcga acgtcaactt gagcaataag
    15721 agcggcctca ccccactcca cctggcggct caagaagacc gagtgaacgt ggctgaggtc
    15781 cttgtcaacc aaggggccca cgtggatgca cagacaaaga tgggctacac gccgctccat
    15841 gtgggctgtc actatggaaa catcaaaata gtcaattttc tgctgcagca ttctgcgaaa
    15901 gttaatgcca agacaaagaa tggatacacg ccactgcacc aggcagccca gcagggccac
    15961 acacacatca tcaacgtcct gctccagaac aacgcttccc ccaatgaact cactgtgaat
    16021 gggaacacag ccctggccat cgcccgacgc cttggctaca tctcggtggt tgacacccta
    16081 aaggtcgtga cagaggaaat tatgaccacc actaccatca cggagaagca caaaatgaat
    16141 gtcccagaaa caatgaatga agtcctcgac atgtcagacg atgaagttgg taaagccagc
    16201 gcccccgaaa agctcagtga tggggaatat atctcagatg gtgaagaagg tgaagatgcc
    16261 atcacagggg acactgacaa gtatctcggg ccacaggacc tcaaggagct gggcgatgac
    16321 tccctgcctg ccgagggcta tgtgggcttc agtctcggag cccgttctgc cagccttcgc
    16381 tccttcagtt cggataggtc ctacaccctg aacagaagct cctatgcgcg ggacagcatg
    16441 atgatcgagg agcttctggt gccatccaaa gagcagcacc ttccattcac gagggagttt
    16501 gactccgact ccctcagaca ctacagttgg gcagcagaca cactggacaa tgtgaacctg
    16561 gtctccagcc ctgtgcattc tgggtttctg gttagcttta tggtggacgc gagagggggc
    16621 tccatgcgag gaagccgcca ccacgggatg cggatcatca tccctccgcg caagtgtacg
    16681 gcccccaccc gcatcacttg ccgcctggta aagagacata aactggccaa cccacccccc
    16741 atggtggaag gagagggatt agccagtagg ctggtagaaa tgggtcctgc gggggcacaa
    16801 tttttaggcc cggtcatagt ggaaatccct cattttgggt ccatgagagg aaaggagagg
    16861 gaactcattg tcctccggag cgagaacgga gagacctgga aggaacacca gtttgacagc
    16921 aaaaacgaag acctctcgga gctcctcaat ggcatggacg aagaacttga cagcccagaa
    16981 gagttgggca caaagcgcat ctgcagaatc atcacgaagg atttccccca gtatttcgct
    17041 gtggtttccc ggattaagca ggaaagcaac cagattggtc ccgaaggtgg gattctgagc
    17101 agcaccacgg tccccctcgt ccaggcgtct ttcccagagg gtgccttaac caaaaggatc
    17161 cgtgtgggtc tgcaggctca gcccgtacca gaggagactg tgaaaaagat ccttggaaac
    17221 aaagcaacgt ttagcccaat tgtcacggtg gagccgagga ggaggaaatt ccataagccg
    17281 atcaccatga ccatcccggt gcccccaccc tcgggagaag gcgtgtccaa tgggtacaag
    17341 ggggacacca cgcccagcct gcgactcctc tgcagcatta caggagggac ctcgccggct
    17401 cagtgggaag acatcacagg cacaaccccg ctgaccttca taaaggactg tgtgtctttc
    17461 acaaccaatg tttcagccag gttctggctg gcggactgcc atcaggtgtt agaaaccgta
    17521 gggctagcct cgcagctgta cagagagttg atatgtgtcc cctacatggc caagtttgtt
    17581 gtttttgcca aaacaaacga cccagtggaa tcctccctaa ggtgcttctg tatgacagac
    17641 gacagagtgg acaaaaccct ggagcagcag gagaatttcg aggaggttgc cagaagcaaa
    17701 gacatcgagg ttctggaagg aaagcccatc tatgttgatt gctatggaaa cctggctcct
    17761 ctgaccaaag gagggcagca gcttgttttt aacttctatt ctttcaaaga aaacagactg
    17821 ccattttcca tcaaggttag agacaccagc caagagccct gcggccgtct gtctttcctg
    17881 aaggagccga aaacaacaaa aggattaccc caaacggctg tttgcaactt aaatattact
    17941 ctaccggcgc ataaaaaggc tgagaaggca gacagacgcc agagcttcac atccctagcc
    18001 ttacgtaagc gctacagcta cttgactgag cccagcatga aaacagttga acggagttca
    18061 ggaacagcaa gatcccttcc cactacttat tcacacaagc cattcttttc tacaagacca
    18121 taccagtcct ggactacgac tccactcaca gtgcccgggc cggccaaatc aggctcctta
    18181 tccagctccc cctctaacac gccgtcagcg tctccgttaa aatccatatg gtctgtttca
    18241 acgccttctc cgatcaaatc cacgctaggc gcctcaacta cgtcatcagt caaatccatt
    18301 agtgatgtcg cctctccaat aaggtctttc cggacaattt cttcgccaat aagaacggtg
    18361 gcatcgccgt ctccatacaa tacccaagtt gcctctggta cccttgggag ggttcccacc
    18421 atcacagagg ccacacctat aaaggggctg gctcccaact caactttgtc ctctcgaact
    18481 tctcctgtga cgaccgcagg gtctctgttg gagaggtcgt ccatcaccat gacaccccct
    18541 gcctccccca aagccaacat caccatgtat tcctccagct tgccatttaa gtccattatc
    18601 acgtcagcgg caccgctgat ctcctcccct ttaaagtcag tggtgtctcc gaccaagtct
    18661 gcagctgatg tcatctcaac agctaaagct gcgatggcat cgactctctc ctccccctta
    18721 aagcagatgt ctggacatgc agaagtagcg ctagtcaatg ggtctgtttc tcctctgaag
    18781 tatccttcct cttccgcttt
//
