LOCUS       sequence-495016-       18826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of mouse ANK3 480 isoform without microexon E35a with
            N terminal flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18826)
  TITLE     Yang lab plasmids 2025
REFERENCE   2  (bases 1 to 18826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAG480_E35a-GFP
FEATURES             Location/Qualifiers
     source          1..18826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3436..3462
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HLHHHHHHH"
     CDS             3442..3468
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHYH"
     CDS             3445..3471
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHYHH"
     RBS             3496..3504
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      4679..4688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4685..4687,4688..4690,4691..5404)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4685..4687,4688..4690,4691..5401)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4730..4751)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4991..5010)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5338..5359
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5527..5648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5564..5583)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5618..5637
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5655..6110)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5792..5813)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6004..6023
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6137..6241
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6243..6600
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6243..6434
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6266..6286)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6451..6586
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6635..7429
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6635..7429
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6689..6708)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7299..7318
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7617..7636)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7661..7708
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8037..8625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8526..8545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8800..9103
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     10321..10343
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10405..10424
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 caaaagcagt gagatggttg tgagcgaatc tggctccagt gatggcaaaa gccacagaac
       61 tgagatgtta agtaaggcaa cgcctgactt ctttcctgag caacaggttg aagactcagc
      121 ctgtcccata acctcagatc tagagaccaa gggaccatgg gaccgaaagg tctttagaac
      181 atgggaaagt tctggggcca ataacaataa ggctcagaaa gaacaactct ctcacgtgct
      241 cgttcacgac atcagagaga gtcacgttgg tcaccctgac agtagcgaga acggtgaccc
      301 aaagaatgat tttatgtatg tgactgagag agaacacaaa atgttaacga acggctctct
      361 ctcagaaatt aaagaaatga gtgtaaagtc tccctccaag aaagtgttat acagggaata
      421 catggttaaa gaaggggacc ttcccagcag cactctcgac ccccccagga gaagcgagag
      481 ctcggcagca tctcacactc ccatcagggt cacagatgag aggaggatgc tgtcatcaaa
      541 cattcctgat gggatttgtg agcagacaac atttccaaaa caagacctgt cccaaagagt
      601 gtcccggcca agtgtgagca aaggggcagt tgagagccag cactttaact ccatagacga
      661 tgagaaagtt acctattccg agatgagcaa agtttccaaa cgtcagagtt acgtaggcct
      721 atgcccagct ctcgatgaaa ccgagacctc tcccaccaaa tctcccgatt ccttagagtt
      781 tagcccaggg aaggactctc cctctagcga tgtgttcgac cacggttcaa tggatgggtt
      841 agagaaggct gaaggaggta aagagatcaa aaccttacct gtttatgtca gcttcgtgca
      901 agtggggaaa cagtatgaaa aggaactcca acaaggaggt gtgaaaaaaa tcatcagtca
      961 ggagtgtaag acagtgcagg aagccagggg gactttctat accgctagac agcagaagca
     1021 gcctccttct ccccagggaa gtccagaaga cgatactcta gagcaagtgt cctttctaga
     1081 cagctctggg aagagccctt tgaccccaga aactcccagt tccgaggaag tgagttatga
     1141 gttcacgtct aagacgccag actcactcat agcttttata ccaggacagc ccagcccaat
     1201 tcctgaggtt tctgaggagg aggaggagga ggaagagccc aaagcagccc cactaaggca
     1261 ggtgcccgtg gagaaggaaa cagatcgtga tgtgagcaaa gactctgtcc aaagacccaa
     1321 atgcaaccga gtggcctata ttgagtttcc tcctcctccc cccctggatg ccgaccagat
     1381 ggagtcagat aagaaacacg agtatctccc cgacagggag gttgacatga tggaagtcag
     1441 ccttcaggat gagtctgaca agtaccagtt ggccgagcca gtcatccgag tgcagccccc
     1501 atcaccagtc cctcctggtg ccgatgccag tgagtccagc gatgacgagt ccatgtatca
     1561 accggcccca gttaaaaaat acaccttcaa gctgaaggga gtggacgaag ggcagaaaga
     1621 cacagcaaaa tccaaaacgg ccaccacaaa ggcttccaac cagaaagagg tggagagtaa
     1681 cgggaaagag agtgagtctg gccttgactc acctcagaat gaaaccgctc agaacggaaa
     1741 caacgaccag tctgtcaccg agtgttccat cgccaccacg gccgagtttt ctcacgacac
     1801 ggatgccaca gagatagact ctctggatgg ctatgacctg caggacgagg atgacggcct
     1861 aacagagagc gactctaaac tcccgagtca aactatagac accaagaagg atgtctggac
     1921 ggagggcatt ctgaagccgg ccgaccgctc tttcagtcag agcaaactgg aagttattga
     1981 agaggagggg aaagtgggac tggacgaaga gaaaccatta ccttctaaaa gctcgtcatc
     2041 tgaccgaacc ccagagaagg ctgatcccaa gtcgggggcc cagtttttca ccctggaagg
     2101 cagacacccg gacagatcag tgttccctga tacttacttc agttacaaag ttgatgaaga
     2161 atttgccact ccctttaaaa cagtggcgac caaaggtcta gactttgacc cttggcccaa
     2221 taaccgaggg gacaatgaag tttttgatgg taaatcccgg gaagatgaca ctaagccatt
     2281 tggtctggcg gtggacgacc gctctccggc aaccacccct gacacaacgc cagccagaac
     2341 accaactgac gaaagtaccc caactagtga gcctaacccc ttcccatttc atgaaggaaa
     2401 aatgtttgag atgacccgca gtggtgcaat tgacatgagc aagagagatt ttgtggaaga
     2461 gaggctccaa tttttccaga ttggtgagca tactcctgaa gggaagtcag gggcccaggg
     2521 ggaagggggt acggtcactg ataccccaca gccagagtca ggggacacct cggtagacac
     2581 caatctagag agagatgtag aggcacctac agtcgacccc aaccccagca tcccgaccaa
     2641 tggagagtgt caggaaggca cagcctgcaa tggctgcctg gagaagtcgg cagctgccac
     2701 taacacctca aaagttgacc ccaagtcacg cacacctata aaaatgggaa tttctgcatc
     2761 taccatgacc atgaagaaag aaggctctgg agacacaaca gataagacag aggctgtggt
     2821 gaccagttat cagggattag aaaatgaaat cgtgaaagag atctcaagcg cactcaatag
     2881 ccaggtgggc attaggcccc aagagaaaca cgattttcaa aaagataact ttaataacaa
     2941 caataatctg gatgcttccg ctatgcagac agataacacc acaagtcaca tagttctgac
     3001 aggacgcgct gcatccactt gtaccacaga gaaagctaat ccagtgaaag gctcaggaaa
     3061 gagtccaggg actcaaggac gctccagcag agattctcag aaggaaccaa taggggttag
     3121 gcggaaatcc aagcttcccg taaaggccac cgcaccaaaa gatgtcttcc cgccaaacca
     3181 taagccagac agtaaagcag gtaaactgag gcaggttggt caatatgaga aaaacaaagc
     3241 ccttcctact tcttcatgta tagattccaa gtctcgaatt ccagtcaaaa acacacacag
     3301 ggagaaccta gtttcagtca gaaaagcatg cgccacacaa aagcgagggc agccagagaa
     3361 aggcaaggcc aaacagcctc catccaagtt gccagtaaag gtaagatcca cctgtgtcac
     3421 cgtcaccacc accaacacct ccaccaccac caccaccacc actaccacca ccgttaaagt
     3481 tacagaaagt cagcgtaagg aggtatgtaa acatcccatt gaatatttta agggaattag
     3541 tggcgagacc ctaaaacttg tggaccgcct cacggaagaa gacagaaaga tgcaatcgga
     3601 gttgtctgat ggggaggaaa gtacctcaag gagcacatcg ctgtccgaga cttcccgggg
     3661 cggccagcct tcagttacca cgaagtctgc tagaaataag agaacagagg caccgccctt
     3721 aaaatcaaag agaggaaaga gctgcagtag gaggactgtt ccacagagtc cttgtgagcg
     3781 gacggatatc aggatggcaa tagtagccga tcacctggga cttagttgga cagagctggc
     3841 aagggaactg aatttttcag tggatgaaat caaccaaata cgagtggaaa atcccaattc
     3901 tttaatttct cagagcttca tgttattaaa aaagtgggtg acgagagacg ggaagaatgc
     3961 cacaactgat gccttaactt cggtcttaac aaaaattaac cgaatagaca tagtaactct
     4021 gctggaagga ccaatatttg attatgggaa tatttcaggc accagaagct ttgcagatga
     4081 aaacaatgtt ttccatgacc cagttgatgg ttggcagaac gagacgccaa gtggaagcct
     4141 agagtccccg gcgcaagcgc gaagaataac cggtgggtta ctggaccgtc tggacgacag
     4201 ctctgaccag gttcgggatc ccattacctc atacctcaca ggagaagctg ggaagttcga
     4261 agcaaacgga aaccacgcag aagtcattcc agaagcaaag gccaaggcct actttcctga
     4321 atcccaaaac gatataggga aacagagcat caaggagaac ttgaaaccaa aaacacacgg
     4381 atgtggtcgc gctgaggaac cagtgtcgcc cctcacagcc taccagaaat ctctagaaga
     4441 aaccagcaag cttgtcatag aagacgcacc taaaccctgt gtgcctgtcg gcatgaaaaa
     4501 gatgaccagg actccggctg acggcaaagc caggctcaac ctccaggaag aggaggggtc
     4561 cgccaggtca gagcctaagc agggagaagg ctataaggtg aaaacaaaga aggaaatccg
     4621 gaatgtggag aagaaagccc accagtcgac ggtaccgcgg gcccgggatc caccggtcgc
     4681 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     4741 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     4801 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     4861 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     4921 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     4981 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     5041 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     5101 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     5161 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     5221 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     5281 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     5341 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     5401 gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
     5461 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     5521 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5581 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5641 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     5701 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     5761 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     5821 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     5881 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     5941 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     6001 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     6061 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     6121 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6181 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6241 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6301 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     6361 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6421 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6481 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6541 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     6601 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     6661 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     6721 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     6781 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     6841 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     6901 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     6961 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     7021 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     7081 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     7141 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     7201 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     7261 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     7321 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     7381 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     7441 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     7501 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     7561 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     7621 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     7681 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     7741 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     7801 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     7861 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     7921 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7981 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8041 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8101 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8161 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8221 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8281 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8341 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8401 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8461 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8521 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8581 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8641 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8701 tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat
     8761 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     8821 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     8881 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     8941 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     9001 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     9061 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
     9121 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
     9181 tttttaatta ttttgtgcag cgatgggggc gggggggggg gggggggcgg gccagggggg
     9241 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggcgaaaac
     9301 agggggggcg gccccgagag tgccctttta gggggggggg gggggggggg gggggcggaa
     9361 aaaaaagagg cgcgggggcg gggggggggg cgggcggccc gcgcccccct cgccgctccg
     9421 ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
     9481 ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt
     9541 cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga
     9601 gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc
     9661 tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc
     9721 gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca
     9781 aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg
     9841 ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg
     9901 cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg
     9961 tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg
    10021 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
    10081 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
    10141 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
    10201 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
    10261 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
    10321 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
    10381 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
    10441 gcaaagaatt catggctcat gccgcctccc agttaaagaa aaacagggat ttagaaatca
    10501 atgcggaaga agagaccgag aaaaaaaaga agcaccgtaa acggtcccgg gatcgcaaga
    10561 aaaagtcgga tgccaatgca agttacttga gagcagctcg ggcggggcac ctggaaaagg
    10621 cccttgacta catcaaaaat ggagtggacg tcaacatctg taaccagaat gggttgaatg
    10681 cgctccatct tgcttccaaa gaaggccatg tggaagtggt ctccgagctg ctgcagagag
    10741 aagccaacgt cgatgcagcc acaaagaaag gaaacacagc cttacacatc gcatccttgg
    10801 ctggacaagc ggaagtggtc aaggtcttgg ttacgaacgg agcgaacgtc aacgcacagt
    10861 ctcagaatgg cttcacgccg ctgtatatgg cggcccagga gaaccacctg gaagtcgtca
    10921 ggtttcttct ggacaatggt gccagccaaa gcctggctac agaggatggc ttcacgccat
    10981 tggccgtggc tcttcaacaa ggtcacgacc aagtcgtatc cctcctgctc gagaacgaca
    11041 cgaagggaaa agtgcgcctc ccagccctcc acatcgcggc ccggaaagat gacaccaaag
    11101 cggcagctct gctcctacag aacgacacca atgcagacat agagtcaaag atggtggtga
    11161 atagagcaac tgagagtggc ttcacctcac tccacatagc tgcccactac gggaatatca
    11221 atgtggccac gttgctgtta aaccgagcgg ctgccgtgga cttcaccgca cggaatgaca
    11281 tcactccttt gcatgttgcc tcgaaacggg gaaacgcaaa tatggtaaag ctgttgctcg
    11341 accggggtgc gaagatcgac gccaagacca gggacggtct gaccccgctg cactgcgggg
    11401 cgagaagtgg ccacgagcag gtggtagaga tgttgctgga cagagccgcc cccatccttt
    11461 cgaaaaccaa gaatggactg tccccactgc acatggccac acaaggagac catttaaact
    11521 gcgtccaact cctcctccag cacaacgtgc ccgtggatga cgtcaccaac gactacctga
    11581 ccgccctcca cgtggcggcc cactgcggcc attacaaagt tgccaaggtt cttttggata
    11641 agaaagctaa ccccaatgcc aaagccctga atggcttcac ccctctccat atcgcctgca
    11701 aaaagaaccg aatccgagta atggaactcc ttttgaagca cggtgcatct attcaagccg
    11761 taaccgagtc gggccttacc ccaatccatg tcgctgcctt catgggacat gtaaatatcg
    11821 tgtcacagct aatgcatcat ggagcctccc caaacaccac caacgtgaga ggagaaacag
    11881 cattgcatat ggcggctcgg tcggggcaag cagaagtggt gcggtatttg gtacaagatg
    11941 gagctcaggt agaagcaaaa gctaaggatg accagacccc actccacatt tcagcccgac
    12001 tcgggaaggc tgacatagtg caacaactgt tacagcaagg agcgtccccg aatgcagcaa
    12061 caacttccgg gtacaccccc cttcaccttt cggccagaga ggggcatgag gatgtagctg
    12121 ccttccttct ggaccatgga gcatctttat ccataacaac aaagaaggga ttcacgcctc
    12181 tgcatgtggc tgcaaagtat gggaagcttg aagtggcaag tctcctgctg cagaagagtg
    12241 cgtctcctga tgccgctggg aagagcgggc taactccact gcatgtagca gcacattacg
    12301 ataatcagaa agtggccctt ctgcttttgg accagggagc ctcaccccac gcagctgcaa
    12361 agaacggtta tacgccactg cacatcgccg ccaagaagaa ccagatggac atagccacgt
    12421 ccctgctgga gtacggtgct gatgcaaacg ccgttacccg gcaagggatt gcgtccgtcc
    12481 accttgcagc gcaggaaggg cacgtggaca tggtgtcgct gctcctcagt agaaatgcga
    12541 acgtcaactt gagcaataag agcggcctca ccccactcca cctggcggct caagaagacc
    12601 gagtgaacgt ggctgaggtc cttgtcaacc aaggggccca cgtggatgca cagacaaaga
    12661 tgggctacac gccgctccat gtgggctgtc actatggaaa catcaaaata gtcaattttc
    12721 tgctgcagca ttctgcgaaa gttaatgcca agacaaagaa tggatacacg ccactgcacc
    12781 aggcagccca gcagggccac acacacatca tcaacgtcct gctccagaac aacgcttccc
    12841 ccaatgaact cactgtgaat gggaacacag ccctggccat cgcccgacgc cttggctaca
    12901 tctcggtggt tgacacccta aaggtcgtga cagaggaaat tatgaccacc actaccatca
    12961 cggagaagca caaaatgaat gtcccagaaa caatgaatga agtcctcgac atgtcagacg
    13021 atgaagttgg taaagccagc gcccccgaaa agctcagtga tggggaatat atctcagatg
    13081 gtgaagaagg tgaagatgcc atcacagggg acactgacaa gtatctcggg ccacaggacc
    13141 tcaaggagct gggcgatgac tccctgcctg ccgagggcta tgtgggcttc agtctcggag
    13201 cccgttctgc cagccttcgc tccttcagtt cggataggtc ctacaccctg aacagaagct
    13261 cctatgcgcg ggacagcatg atgatcgagg agcttctggt gccatccaaa gagcagcacc
    13321 ttccattcac gagggagttt gactccgact ccctcagaca ctacagttgg gcagcagaca
    13381 cactggacaa tgtgaacctg gtctccagcc ctgtgcattc tgggtttctg gttagcttta
    13441 tggtggacgc gagagggggc tccatgcgag gaagccgcca ccacgggatg cggatcatca
    13501 tccctccgcg caagtgtacg gcccccaccc gcatcacttg ccgcctggta aagagacata
    13561 aactggccaa cccacccccc atggtggaag gagagggatt agccagtagg ctggtagaaa
    13621 tgggtcctgc gggggcacaa tttttaggcc cggtcatagt ggaaatccct cattttgggt
    13681 ccatgagagg aaaggagagg gaactcattg tcctccggag cgagaacgga gagacctgga
    13741 aggaacacca gtttgacagc aaaaacgaag acctctcgga gctcctcaat ggcatggacg
    13801 aagaacttga cagcccagaa gagttgggca caaagcgcat ctgcagaatc atcacgaagg
    13861 atttccccca gtatttcgct gtggtttccc ggattaagca ggaaagcaac cagattggtc
    13921 ccgaaggtgg gattctgagc agcaccacgg tccccctcgt ccaggcgtct ttcccagagg
    13981 gtgccttaac caaaaggatc cgtgtgggtc tgcaggctca gcccgtacca gaggagactg
    14041 tgaaaaagat ccttggaaac aaagcaacgt ttagcccaat tgtcacggtg gagccgagga
    14101 ggaggaaatt ccataagccg atcaccatga ccatcccggt gcccccaccc tcgggagaag
    14161 gcgtgtccaa tgggtacaag ggggacacca cgcccagcct gcgactcctc tgcagcatta
    14221 caggagggac ctcgccggct cagtgggaag acatcacagg cacaaccccg ctgaccttca
    14281 taaaggactg tgtgtctttc acaaccaatg tttcagccag gttctggctg gcggactgcc
    14341 atcaggtgtt agaaaccgta gggctagcct cgcagctgta cagagagttg atatgtgtcc
    14401 cctacatggc caagtttgtt gtttttgcca aaacaaacga cccagtggaa tcctccctaa
    14461 ggtgcttctg tatgacagac gacagagtgg acaaaaccct ggagcagcag gagaatttcg
    14521 aggaggttgc cagaagcaaa gacatcgagg ttctggaagg aaagcccatc tatgttgatt
    14581 gctatggaaa cctggctcct ctgaccaaag gagggcagca gcttgttttt aacttctatt
    14641 ctttcaaaga aaacagactg ccattttcca tcaaggttag agacaccagc caagagccct
    14701 gcggccgtct gtctttcctg aaggagccga aaacaacaaa aggattaccc caaacggctg
    14761 tttgcaactt aaatattact ctaccggcgc ataaaaagga gacagagtca gatcaagatg
    14821 atgaggctga gaaggcagac agacgccaga gcttcacatc cctagcctta cgtaagcgct
    14881 acagctactt gactgagccc agcatgaaaa cagttgaacg gagttcagga acagcaagat
    14941 cccttcccac tacttattca cacaagccat tcttttctac aagaccatac cagtcctgga
    15001 ctacgactcc actcacagtg cccgggccgg ccaaatcagg ctccttatcc agctccccct
    15061 ctaacacgcc gtcagcgtct ccgttaaaat ccatatggtc tgtttcaacg ccttctccga
    15121 tcaaatccac gctaggcgcc tcaactacgt catcagtcaa atccattagt gatgtcgcct
    15181 ctccaataag gtctttccgg acaatttctt cgccaataag aacggtggca tcgccgtctc
    15241 catacaatac ccaagttgcc tctggtaccc ttgggagggt tcccaccatc acagaggcca
    15301 cacctataaa ggggctggct cccaactcaa ctttgtcctc tcgaacttct cctgtgacga
    15361 ccgcagggtc tctgttggag aggtcgtcca tcaccatgac accccctgcc tcccccaaag
    15421 ccaacatcac catgtattcc tccagcttgc catttaagtc cattatcacg tcagcggcac
    15481 cgctgatctc ctccccttta aagtcagtgg tgtctccgac caagtctgca gctgatgtca
    15541 tctcaacagc taaagctgcg atggcatcga ctctctcctc ccccttaaag cagatgtctg
    15601 gacatgcaga agtagcgcta gtcaatgggt ctgtttctcc tctgaagtat ccttcctctt
    15661 ccgctttaat taacggatgc aaagccactg ccacattaca ggacaaaatt tctacaccca
    15721 caaatgctgt gagctcggtg gtgagcgcgg cccctgacac ggtggagaaa gcgctctcca
    15781 ccacaacagc catgcccttt tccccactca ggtcttacgt ttccgctgca gcgccctctg
    15841 cttttcagtc ccttagagct ccctctgcca gtgcactcta cacctccctc gggccttcag
    15901 tagctgttac tacctcatcc gtaacttcat cgataatcac agtgccagta tactcggtag
    15961 tcaatgtttt gccagaacca gcattgaaga aacttccaga ctctaactcg ctcacaaaat
    16021 cggcagcggc tttgctgtcg cccattaaaa cattgactac ggagacacgt cctcagcccc
    16081 atttcaatcg aacgtcatct cccgtcaagt cctctctgtt cctggcatcc tcggccctta
    16141 agccgtcagt accatcttct ttatcttcca gtcaggagat cttaaaagat gtggccgaga
    16201 tgaaggagga cctcatgaga atgaccgcca tactgcagac ggatgtgcct gaggagaagc
    16261 cattccagac cgacctcccc cgagaaggga ggatagacga cgaagagcct ttcaaaatcg
    16321 ttgagaaagt gaaggaagac ttagtcaaag tcagtgagat actcaaaaag gacgtgtgtg
    16381 tcgagagcaa agggccaccc aagtccccaa agagtgacaa aggacactct ccagaagatg
    16441 actggacgga atttagttct gaggaaatac gagaagccag gcaggctgct gccagtcacg
    16501 ccccatccct gcccgagaga gtacatggaa aggccaacct caccagagtc atagactacc
    16561 tgaccaatga catcgggagc agttcactga ccaacttaaa gtacaagttt gaagaggcta
    16621 agaaggaagg agaggagaga cagaagagaa ttttaaagcc agccatggcc ttgcaggagc
    16681 ataaactcaa aatgccgcca gcctccatga gaccttccac ctccgagaag gaactgtgca
    16741 agatggcgga ctcctttttt ggaacagatg ccatcctaga gtctcccgat gacttttctc
    16801 aacatgacca agacaaaagt cccttgtccg acagtggctt tgaaacccga agtgagaaaa
    16861 caccttcagc cccccaaagt gccgagagca ccggccctaa gccactattt catgaagtcc
    16921 ccatcccccc tgtcatcaca gagacgagaa ctgaagtagt ccacgtgatc aggagctacg
    16981 agccctctac tggggagatc ccccagtccc agccagagga ccccgtgtct cccaaacctc
    17041 cccctacatt tatggagttg gaaccaaagc ccaccgccct tagcattaag gaaaaagtga
    17101 aagcattcca aatgaaagcc agtagcgagg aagaggacca cagtcgggtt ttaagcaaag
    17161 gcatgcgtgt caaagaagag actcacatca ccacaaccac gaggatggtg tatcactccc
    17221 cgcctggcag tgagtgtgcc tccgagagga ttgaggagac catgtcagtc catgacatta
    17281 tgaaggcttt tcagtccggg cgagaccctt ccaaagagct ggcggggctg tttgaacaca
    17341 agtcggccat gtctccggat gttgccaagt ctgctgctga gacctcggcc cagcacgcag
    17401 agaaggacaa ccaaatgaaa cccaaactgg agcgtattat agaggtccac atcgaaaaag
    17461 gtaaccaagc cgaacccact gaagtcatta ttagagagac caaaaagcat ccagagaagg
    17521 aagtgtctgt gtatcagaaa gacttattat cccggggaaa tattaactta aaagattttc
    17581 tgccagagaa gcacgatgct ttcccttgcc ccgaggagca gggccagcaa gaagaggagg
    17641 agtttgccgc agaagagtcc ttgccttctt atctggagtc ttccagagta aacactccag
    17701 ggtcccaaga agaagatagc cgccctagtt ccgcacagct tctgtccgat gactcttata
    17761 aaaccctgaa gcttctgagt cagcactccg tagagtacca tgatgatgag ctgtcagacc
    17821 taagagggga gtcttacagg tttgccgaga aaatgcttct ctcagaaaag ctagatgttt
    17881 ctcactctga taccgaggaa tcggtgacag accatgcagg accccctagc tcagagttac
    17941 aggggtctga taagcgatcc agagaaaaag tagccactgc ccccccaaaa gagatcctct
    18001 ccaaaatcta taaagatgtg tctgaaaatg gtatagggag agtgtctaaa gatgagcatt
    18061 ttgagaaact gacagtgttg cactacgcgg gcaatgtcag tggtcccaaa cataccatgt
    18121 ggatgcattt gtccgaggac agactagaca gaggtagaga gaagctgatg tatgaagata
    18181 gggtggacag gaccgtgaag gaggcagaag agaagttgac cgaagtgtct caattttttc
    18241 gtgacaaaac cgagaagctc aacgacgaac tgcagtcccc ggagaaaaag ccacgcccca
    18301 aaaacggcaa ggaatactct tctcagagtt ccaccagcag tagccccgag aaagtgttaa
    18361 cagaactgtt ggcatccaac gatgaatggg ttaaggccag gcagcgcggt ccagatggac
    18421 aaggtgtccc gcaggctgag gacaggaagg cacccagtgg gtccagcagc cccgagaaga
    18481 gggtcccttc ccaacagatt gaggatggcc agcctccaga ggaagccaaa agaactgtca
    18541 ttgctcagag caggggacaa gaaggacccc agtctggatt tcagctcaaa caatctaaac
    18601 tcagttccat tagattaaaa tttgaacaag gcgcacgggc caaaagtaag gatcatgagg
    18661 aaaaaaatct ggacgggcca tcccgaatcc ctgtaaagaa aactcaagaa aacaagctgc
    18721 ccacgcaccc aggttttgct agagaaaaac agcagaaggc tgtagatccc ccagaggaaa
    18781 aaatgtcggt gcaaaatgat gttctgattt tcagacctga gcatgg
//
