LOCUS       sequence-495018-       10626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AID 2.0's higher degradation efficiency came with target-specific 
            basal degradation and slower recovery rates. To address these 
            limitations, we employed base-editing-mediated mutagenesis followed 
            by s.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Xing D, Bai T, Neyisci O, Paylakhi SZ, Duval AJ, Tekin Y, Adli M
  TITLE     Systematic comparison and base-editing-mediated directed protein 
            evolution and functional screening yield superior auxin-inducible 
            degron technology.
  JOURNAL   Nat Commun. 2025 Jul 18;16(1):6631. doi: 10.1038/s41467-025-61848-1.
  PUBMED    40681502
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-18"; volume: "16(1):6631. doi"; pages: " 
            10.1038/s41467-025-61848-1"
COMMENT     Sequence Label: AAVS1_CAG_OsTIR1_F74G_S210A
FEATURES             Location/Qualifiers
     source          1..10626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    129..150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(165..170,171..188,189..195)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    203..219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     208..230
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     227..243
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     227..243
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        264..282
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    317..1120
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     enhancer        1175..1478
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1480..1756
                     /label=chicken beta-actin promoter
     intron          1757..2773
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2697..2719
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2781..2800
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4610..4666
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4688..5287
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4688..4707)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5184..5204
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5314..5902
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5367..5387)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5785..5796)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5938..6059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5975..5994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6029..6048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6086..6119
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6150..6986
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7020..7039)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7021..7039)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7046..7063)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7046..7062)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7055..7077)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7200..7502
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7381..7400
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             7851..8645
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7851..8645
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7905..7924)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8515..8534
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(8895..9686,9687..9755))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9518..9537
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9879..10467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10368..10387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(10621..10626,1..12)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
       61 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      121 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      181 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
      241 gaccatgatt acgccaagct cagaattaac cctcactaaa gggactagtc ctgcaggttt
      301 aaacgaattc gccctttgct ttctctgacc agcattctct cccctgggcc tgtgccgctt
      361 tctgtctgca gcttgtggcc tgggtcacct ctacggctgg cccagatcct tccctgccgc
      421 ctccttcagg ttccgtcttc ctccactccc tcttcccctt gctctctgct gtgttgctgc
      481 ccaaggatgc tctttccgga gcacttcctt ctcggcgctg caccacgtga tgtcctctga
      541 gcggatcctc cccgtgtctg ggtcctctcc gggcatctct cctccctcac ccaaccccat
      601 gccgtcttca ctcgctgggt tcccttttcc ttctccttct ggggcctgtg ccatctctcg
      661 tttcttagga tggccttctc cgacggatgt ctcccttgcg tcccgcctcc ccttcttgta
      721 ggcctgcatc atcaccgttt ttctggacaa ccccaaagta ccccgtctcc ctggctttag
      781 ccacctctcc atcctcttgc tttctttgcc tggacacccc gttctcctgt ggattcgggt
      841 cacctctcac tcctttcatt tgggcagctc ccctaccccc cttacctctc tagtctgtgc
      901 tagctcttcc agccccctgt catggcatct tccaggggtc cgagagctca gctagtcttc
      961 ttcctccaac ccgggcccct atgtccactt caggacagca tgtttgctgc ctccagggat
     1021 cctgtgtccc cgagctggga ccaccttata ttcccagggc cggttaatgt ggctctggtt
     1081 ctgggtactt ttatctgtcc cctccacccc acagtggggc aagctagtaa tcaattacgg
     1141 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     1201 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     1261 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     1321 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     1381 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     1441 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
     1501 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     1561 aattattttg tgcagcgatg ggggcggggg gggggggggg cgcgcgccag gcggggcggg
     1621 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
     1681 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     1741 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
     1801 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     1861 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
     1921 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
     1981 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
     2041 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     2101 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     2161 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     2221 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     2281 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     2341 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     2401 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     2461 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     2521 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     2581 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     2641 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     2701 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
     2761 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     2821 agaattcgag ctcggtacct cgcgaatgca tctagatagc gctgccacca tgacatactt
     2881 tcctgaagag gtcgtcgaac acatttttag cttcctgcct gcacagagag atagaaacac
     2941 agtgagcctg gtctgcaaag tgtggtacga gatcgaacgc ctgagccgga gaggagtgtt
     3001 cgtcggcaac tgctatgctg tgagagcagg cagggtcgcc gctaggtttc caaatgtgcg
     3061 cgcactgacc gtcaagggga aaccccacgg cgccgacttt aacctggtgc cccctgattg
     3121 gggaggatac gccggccctt ggatcgaggc agccgctcgc ggctgtcatg gactggagga
     3181 actgcgcatg aagcgaatgg tggtctctga cgaaagtctg gagctgctgg ctcggagctt
     3241 ccctaggttt cgcgcactgg tgctgatttc ttgcgaaggc ttcagcaccg atggactggc
     3301 agccgtggcc tcccactgta agctgctgcg ggagctggac ctccaggaga atgaagtgga
     3361 ggatagaggc cccagatggc tgtcttgctt cccagactca tgtaccagcc tggtgtccct
     3421 gaactttgcc tgcatcaaag gcgaagtgaa tgctgggtcc ctggagcggc tggtctcaag
     3481 aagccccaac ctgagggctc tgcggctgaa ccggagcgtg agcgtggaca ctctggctaa
     3541 gattctgctg agaaccccta acctggagga tctgggaacc ggcaatctga cagacgattt
     3601 ccagacagaa tcctacttta aactgacttc tgccctggag aagtgtaaaa tgctgaggag
     3661 tctgtcagga ttctgggatg cttcacccgt gtgcctgagc tttatctacc ctctgtgtgc
     3721 acagctgaca ggcctgaacc tgagctatgc accaaccctg gacgccagtg atctgacaaa
     3781 gatgatctca cgctgcgtga aactccagcg actgtgggtg ctggactgta tttccgataa
     3841 ggggctccag gtggtcgcca gctcctgcaa ggacctccag gagctgagag tgttcccatc
     3901 tgatttttac gtggccggat atagtgctgt cactgaggaa ggcctggtgg cagtctcact
     3961 gggatgccca aagctgaaca gcctgctgta tttctgtcat cagatgacta atgctgcact
     4021 ggtgaccgtc gccaagaact gccctaattt cacccgattt cggctgtgta ttctggaacc
     4081 aggcaaaccc gacgtggtca catcccagcc actggatgaa gggtttggag ctatcgtgag
     4141 agagtgcaag ggactccaga ggctgagcat ttccggcctg ctgacagaca aagtgttcat
     4201 gtacatcggc aagtatgcta agcagctgga gatgctgagc attgcatttg ccggagactc
     4261 cgataagggc atgatgcacg tgatgaacgg gtgtaagaat ctgcgaaaac tggaaatccg
     4321 ggacagccct ttcggggatg ccgctctgct gggaaacttt gccagatacg agacaatgag
     4381 gagcctgtgg atgtctagtt gcaatgtgac tctgaagggc tgtcaggtcc tggctagtaa
     4441 aatgcctatg ctgaacgtgg aagtcattaa tgagcgggac gggtctaacg aaatggagga
     4501 aaatcatggc gacctgccaa aggtggagaa actgtatgtg tatcggacca ccgcaggggc
     4561 aagagatgat gctcccaact ttgtgaagat tctgagatca ggaagcggag ctactaactt
     4621 cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctagat ctagacgtac
     4681 ggccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     4741 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     4801 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     4861 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac
     4921 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     4981 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5041 caaggagccc gcgtggttcc tggccaccgt cggagtctcg cccgaccacc agggcaaggg
     5101 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     5161 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     5221 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     5281 tgcctgaacg aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     5341 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5401 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5461 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5521 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5581 ctcctttccg gaactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5641 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5701 tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc
     5761 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     5821 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     5881 tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgcaattgt tgttgttaac
     5941 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6001 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     6061 gcgatcatat tcaataaccc ttaatataac ttcgtataat gtatgctata cgaagttatt
     6121 aggtccctcg aagaggttca ctaagcttta ctagggacag gattggtgac agaaaagccc
     6181 catccttagg cctcctcctt cctagtctcc tgatattggg tctaaccccc acctcctgtt
     6241 aggcagattc cttatctggt gacacacccc catttcctgg agccatctct ctccttgcca
     6301 gaacctctaa ggtttgctta cgatggagcc agagaggatc ctgggaggga gagcttggca
     6361 gggggtggga gggaaggggg ggatgcgtga cctgcccggt tctcagtggc caccctgcgc
     6421 taccctctcc cagaacctga gctgctctga cgcggctgtc tggtgcgttt cactgatcct
     6481 ggtgctgcag cttccttaca cttcccaaga ggagaagcag tttggaaaaa caaaatcaga
     6541 ataagttggt cctgagttct aactttggct cttcaccttt ctagtcccca atttatattg
     6601 ttcctccgtg cgtcagtttt acctgtgaga taaggccagt agccagcccc gtcctggcag
     6661 ggctgtggtg aggagggggg tgtccgtgtg gaaaactccc tttgtgagaa tggtgcgtcc
     6721 taggtgttca ccaggtcgtg gccgcctcta ctccctttct ctttctccat ccttctttcc
     6781 ttaaagagtc cccagtgcta tctgggacat attcctccgc ccagagcagg gtcccgcttc
     6841 cctaaggccc tgctctgggc ttctgggttt gagtccttgg caagcccagg agaggcgctc
     6901 aggcttccct gtcccccttc ctcgtccacc atctcatgcc cctggctctc ctgccccttc
     6961 cctacagggg ttcctggctc tgctctaagg gcgaattcgc ggccgctaaa ttcaattcgc
     7021 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     7081 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7141 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagccta tacgtacggc
     7201 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     7261 gtgatattat tgacacgccg gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     7321 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     7381 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     7441 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     7501 aaatgtcagg catgagatta tcaaaaagga tcttcaccta gatccttttc acgtagaaag
     7561 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa
     7621 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
     7681 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
     7741 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
     7801 ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     7861 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     7921 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     7981 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     8041 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     8101 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     8161 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     8221 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     8281 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     8341 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     8401 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     8461 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     8521 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     8581 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     8641 tctgaattat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     8701 gtatttcaca ccgcatcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8761 ttatttttct aaatacattc aaatatgtat ccgctcatga gattatcaaa aaggatcttc
     8821 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8881 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8941 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9001 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9061 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9121 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9181 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9241 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9301 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9361 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9421 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9481 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9541 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9601 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9661 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9721 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9781 agcatttatc agggttattg tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9841 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9901 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9961 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    10021 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    10081 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10141 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10201 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    10261 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    10321 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    10381 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10441 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    10501 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    10561 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    10621 agcgag
//
