LOCUS       sequence-495089-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knockdown of endogenous SF3B1 with shRNA targeting 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Baker M, Engal E, Sharma A, Petasny M, Jaffe-Herman S, Geminder O, 
            Su M-H, Bentata M, Kay G, Salton M
  TITLE     SF3B1K700E rewires splicing of cell-cycle regulators
  JOURNAL   RNA (2025, in press)
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA (2025, 
            in press)"
COMMENT     Sequence Label: Tet-pLKO-puro-shSF3B1
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    37..154
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        203..713
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     632..650
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          722..1294
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1302..1321
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1349..1367
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1378..1998
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1451..1470)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    2033..2606
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2200..2217)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2427..2446
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2626..3225
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2626..2645)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3122..3142
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3353..3586
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3658..3779
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3695..3714)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3749..3768
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3819..3954
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(3975..3993)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4003..4020)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4003..4019)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4012..4034)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4161..4616
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4248..4267)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4458..4479
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4642..4746
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4747..4815,4816..5607)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4965..4984)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5778..6366
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6267..6286
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6520..6537
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6654..6675
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6690..6695,6696..6713,6714..6720)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6728..6744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6733..6755
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6752..6768
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6752..6768
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6787..6807
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6789..6807
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6835..7061
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7062..7242
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7289..7414
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7907..8140
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8325..8369
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8658..8752
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     8669..8688
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    8706..8724
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8734..8752
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
       61 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
      121 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
      181 tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc agccctgggt
      241 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
      301 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
      361 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
      421 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
      481 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
      541 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
      601 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
      661 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc
      721 tgtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata
      781 tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca
      841 tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac cattgtctcc
      901 tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg
      961 aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct aatcactttt
     1021 ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga gaacaattgt
     1081 tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc gtggaaatat
     1141 tcttattggt agaaacaact acatcctggt catcatcctg cctttctctt tatggttaca
     1201 atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg gcccctctgc
     1261 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     1321 gctgtctcat cattttggca aagaattgta atacgactca ctatagggcg aattgatatg
     1381 tctagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga
     1441 atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg
     1501 tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat
     1561 aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat
     1621 aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta
     1681 ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc
     1741 caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact
     1801 ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct
     1861 actactgata gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt
     1921 gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt
     1981 aaatgtgaaa gtgggtccgc gtacagcggc tcccgggagt tctagggatc tgcccctctc
     2041 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     2101 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     2161 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     2221 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     2281 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     2341 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     2401 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     2461 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     2521 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     2581 ttttcctttg aaaaacacga tgataaggat ccaccggagc ttaccatgac cgagtacaag
     2641 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     2701 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     2761 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     2821 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     2881 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     2941 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     3001 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     3061 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     3121 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     3181 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
     3241 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggtaccttt aagaccaatg
     3301 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     3361 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     3421 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     3481 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     3541 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     3601 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     3661 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3721 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3781 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3841 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3901 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     3961 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     4021 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     4081 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4141 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4201 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4261 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4321 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4381 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4441 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4501 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4561 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     4621 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4681 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4741 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4801 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4861 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4921 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4981 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5041 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5101 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5161 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5221 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5281 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5341 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5401 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5461 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5521 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5581 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5641 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5701 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5761 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5821 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5881 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5941 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6001 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6061 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6121 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6181 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6241 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6301 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6361 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6421 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6481 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6541 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6601 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     6661 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     6721 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     6781 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     6841 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     6901 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     6961 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     7021 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     7081 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7141 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7201 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     7261 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     7321 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     7381 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     7441 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     7501 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     7561 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     7621 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     7681 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     7741 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     7801 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     7861 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     7921 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     7981 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     8041 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     8101 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     8161 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     8221 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     8281 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     8341 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     8401 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     8461 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     8521 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     8581 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact
     8641 agcctcgagc ggccgcaata tttgcatgtc gctatgtgtt ctgggaaatc accataaacg
     8701 tgaaatccct atcagtgata gagacttata agttccctat cagtgataga gacaccggtg
     8761 ctttgatttg gtgatgtaac tcgagttaca tcaccaaatc aaagcatttt tgaatt
//
