LOCUS       sequence-49514-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Henderson MJ, Russell AJ, Hird S, Munoz M, Clancy JL, Lehrbach GM, 
            Calanni ST, Jans DA, Sutherland RL, Watts CK
  TITLE     EDD, the human hyperplastic discs protein, has a role in 
            progesterone receptor coactivation and potential involvement in DNA 
            damage response.
  JOURNAL   J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13.
  PUBMED    12011095
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-07-19"; volume: "277(29)"; pages: "26468-78. 
            Epub 2002 May 13"
COMMENT     Sequence Label: pCMV-Tag2B EDD
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        43..61
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttagtgaacc gtcagatccg ctagcgatta cgccaagctc gaaattaacc ctcactaaag
       61 ggaacaaaag ctggagctcc accgcggtgg cggccgccac catggattac aaggatgacg
      121 acgataagag cccgggcgga tcccccgggc tgcaggaatt cgatcgagtg gaggacgaga
      181 aggaaagcac catgacgtcc atccatttcg tggttcaccc gctgccgggc accgaggacc
      241 agctcaatga caggttacga gaagtttctg agaagctgaa caaatataat ttaaacagcc
      301 accccccttt gaatgtattg gaacaggcta ctattaaaca gtgtgtggtg ggaccaaatc
      361 atgctgcctt tcttcttgag gatggtagag tttgcaggat tggtttttca gtacagccag
      421 acagattgga attgggtaaa cctgataata atgatgggtc aaagttgaac agcaactcgg
      481 gggcagggag gacgtcaagg cctggtagga caagcgactc tccatggttt ctctcaggtt
      541 ctgagactct aggcaggctg gcaggcaaca ccttaggaag ccgctggagt tctggagtgg
      601 gtggaagtgg tggaggatcc tctggtaggt catcagctgg agctcgagat tcccgccggc
      661 agactcgagt tattcggaca ggacgggatc gagggtctgg gcttttgggc agtcagcccc
      721 agccagttat tccagcatct gtcattccag aggagctgat ttcacaggcc caagttgttt
      781 tacaaggcaa atccagaagt gtcattattc gagaacttca gagaacaaat cttgatgtga
      841 accttgctgt aaataattta cttagccggg atgatgaaga tggagatgat ggggatgata
      901 cagccagcga atcttatttg cctggagagg atcttatgtc tctccttgat gcc
//
