LOCUS       sequence-49520-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Henderson MJ, Russell AJ, Hird S, Munoz M, Clancy JL, Lehrbach GM, 
            Calanni ST, Jans DA, Sutherland RL, Watts CK
  TITLE     EDD, the human hyperplastic discs protein, has a role in 
            progesterone receptor coactivation and potential involvement in DNA 
            damage response.
  JOURNAL   J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13.
  PUBMED    12011095
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-07-19"; volume: "277(29)"; pages: "26468-78. 
            Epub 2002 May 13"
COMMENT     Sequence Label: pEGFP-C1 EDD
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..47
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    191..312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(228..247)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     282..301
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(319..774)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(456..477)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     668..687
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccattaagac caagaatttt ggttttgtgc atcaccatca ccatcactag ccgcgggccc
       61 gggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt
      121 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
      181 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      241 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      301 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
      361 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
      421 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
      481 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
      541 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
      601 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
      661 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
      721 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
      781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      901 a
//
