LOCUS       sequence-495223-        9234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre dependent flag-tagged Rpl22l1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Tanimura A, Du Y, Kondapalli J, Wokosin DL, Surmeier DJ
  TITLE     Cholinergic Interneurons Amplify Thalamostriatal Excitation of 
            Striatal Indirect Pathway Neurons in Parkinson's Disease Models.
  JOURNAL   Neuron. 2019 Feb 6;101(3):444-458.e6. doi: 
            10.1016/j.neuron.2018.12.004. Epub 2019 Jan 15.
  PUBMED    30658860
REFERENCE   2  (bases 1 to 9234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2018.12.004"; journalName: "Neuron"; date: 
            "2019-02-6- 6"; volume: "101"; issue: "3"; pages: "444-458.e6"
COMMENT     Sequence Label: pFB-EF1a-DIO-Rpl22l1-myc-Flag-2A-tdTomato-WPRE...
FEATURES             Location/Qualifiers
     source          1..9234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     357..376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  761..985
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1052..1585)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1755..1760,1761..1777,1778..1783))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   2123..2263
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2134..2263
                     /label=AAV2 ITR
     promoter        2364..3542
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2595..3533
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3490..3510
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(3573..3606)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3657..3690)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3699..5129)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             complement(5130..5183)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5199..5222)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(5241..5270)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      5648..5657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    5664..5697
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    5748..5781
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5806..6394
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5859..5879)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6277..6288)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6426..6902
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6625..6644)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   6942..7082
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6942..7071
                     /label=AAV2 ITR
     mobile_element  7137..7302
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      7486..7940
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7573..7592)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7782..7803
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7966..8070
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8071..8139,8140..8931)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8289..8308)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9102..9234,1..456)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
       61 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
      121 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      181 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      241 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      301 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      361 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      421 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      481 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      541 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      601 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
      661 cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg
      721 gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc
      781 ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt
      841 tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct
      901 aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc
      961 caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg
     1021 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa
     1081 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac
     1141 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga
     1201 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
     1261 agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc
     1321 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
     1381 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac
     1441 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
     1501 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
     1561 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     1621 tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca
     1681 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     1741 gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca
     1801 tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc
     1861 tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg
     1921 ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt caggagatcg
     1981 gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca
     2041 tcctcggttt tctggaaggc gagcatcgtt tgttcgccca ggactctagc tatagttcta
     2101 gtggttggct acagcttgca tgcctgcagg cagctgcgcg ctcgctcgct cactgaggcc
     2161 gcccgggcaa agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
     2221 gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg cacgcgtaag
     2281 ctttgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct
     2341 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
     2401 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     2461 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     2521 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     2581 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     2641 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
     2701 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
     2761 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
     2821 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
     2881 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
     2941 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
     3001 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
     3061 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
     3121 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
     3181 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     3241 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     3301 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     3361 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     3421 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     3481 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     3541 gaggtaccgg atcctctaga gtcgactccg gaataacttc gtataggata ctttatacga
     3601 agttatgcag aatggtagct ggattgtagc tgctattagc aatatgaaac ctcttaataa
     3661 cttcgtatag catacattat acgaagttat ggcgcgcctt acttgtacag ctcgtccatg
     3721 ccgtacagga acaggtggtg gcggccctcg gagcgctcgt actgttccac gatggtgtag
     3781 tcctcgttgt gggaggtgat gtccagcttg gtgtccacgt agtagtagcc gggcagttgc
     3841 acgggcttct tggccatgta gatggtcttg aactccacca ggtagcggcc gccgtccttc
     3901 agcttcaggg cctggtggat ctcgcccttc agcacgccgt cgcgggggta caggcgctcg
     3961 gtggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcggg ggggaagttg
     4021 gtgccgcgca tcttcacctt gtagatcagc gtgccgtcct gcagggagga gtcctgggtc
     4081 acggtcacca gaccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg
     4141 aaggacagct tcttgtaatc ggggatgtcg gcggggtgct tcacgtacgc cttggagccg
     4201 tacatgaact ggggggacag gatgtcccag gcgaagggca gggggccgcc cttggtcacc
     4261 ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg
     4321 aactcgtggc cgttcatgga gccctccatg cgcaccttga agcgcatgaa ctctttgatg
     4381 acggccatgt tgttgtcctc ggaggaggcg gtgccggagc tgccgctgcc ggtgctgccg
     4441 gtgccatgcc ccaggaacag gtggtggcgg ccctcggagc gctcgtactg ttccacgatg
     4501 gtgtagtcct cgttgtggga ggtgatgtcc agcttggtgt ccacgtagta gtagccgggc
     4561 agttgcacgg gcttcttggc catgtagatg gtcttgaact ccaccaggta gcggccgccg
     4621 tccttcagct tcagggcctg gtggatctcg cccttcagca cgccgtcgcg ggggtacagg
     4681 cgctcggtgg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggggggg
     4741 aagttggtgc cgcgcatctt caccttgtag atcagcgtgc cgtcctgcag ggaggagtcc
     4801 tgggtcacgg tcaccagacc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
     4861 tcggggaagg acagcttctt gtaatcgggg atgtcggcgg ggtgcttcac gtacgccttg
     4921 gagccgtaca tgaactgggg ggacaggatg tcccaggcga agggcagggg gccgcccttg
     4981 gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg
     5041 atctcgaact cgtggccgtt catggagccc tccatgcgca ccttgaagcg catgaactct
     5101 ttgatgacct cctcgccctt gctcaccatt gggccaggat tctcctcgac gtcaccgcat
     5161 gttagcagac ttcctctgcc ctctccactg ccagaaacct tatcgtcgtc atccttgtaa
     5221 tccaggatat catttgctgc cagatcctct tctgagatga gtttctgctc gagcggccgc
     5281 gtacgcgtgt cctcagactc tgaaccatct tcatcttgac taatctggaa ataacgaagt
     5341 tcgtaagtct ccttgtcaga tgcgaccaca cgtagccaat cacggagatt attcttctta
     5401 aggtatttct tggtaagata tttcaaatac cttttggaga actgtttctc agaaacaact
     5461 gtgattttat tcttcaggcg ttcaatgtga acaacattgc caagatttcc agtttttcca
     5521 ttgactttaa ccttctcccg tagaaactgt tcaaaatttc cagagtcaaa aattccatct
     5581 tctactggat gagtaaggtc caaatgaaac ctccaggtgg acttcttagg cttcttgtct
     5641 ttcggcgcca tggtggcgct agcataactt cgtataaagt atcctatacg aagttatttg
     5701 ccttaaccca gaaattatca ctgttattct ttagaatggt gcaaagaata acttcgtata
     5761 atgtatgcta tacgaagtta tgaattcgat atcaagctta tcgataatca acctctggat
     5821 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5881 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5941 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6001 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6061 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6121 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6181 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc
     6241 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6301 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6361 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgagcgct gctcgagaga
     6421 tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca
     6481 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
     6541 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga
     6601 agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat
     6661 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
     6721 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
     6781 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
     6841 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
     6901 ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga
     6961 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     7021 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
     7081 ggtctgagac aataaccctg ataaatgctt caataatgta agctctagga gatccgaacc
     7141 agataagtga aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg
     7201 acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc
     7261 atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca tttttcttcc
     7321 tgttatgttt ttaatcaaac atcctgccaa ctccatgtga caaaccgtca tcttcggcta
     7381 ctttttctct gtcacagaat gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa
     7441 ttgactgaat atcaacgctt atttgcagcc tgaatggcga atgggacgcg ccctgtagcg
     7501 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7561 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7621 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7681 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7741 cggtttttcg ccctttgacg ttggagtcca cgttcttaat agtggactct tgttccaaac
     7801 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     7861 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     7921 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7981 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8041 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8101 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8161 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     8221 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     8281 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     8341 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     8401 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8461 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8521 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8581 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     8641 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8701 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     8761 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     8821 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     8881 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     8941 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9001 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9061 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9121 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9181 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcag
//
